Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Fanconi anemia protein FANCI functions in ribosome biogenesis

doi: 10.1073/pnas.1811557116

Figure Lengend Snippet: FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an Agilent 2100 Bioanalyzer. Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.

Article Snippet: To measure the ratio of mature 28S to 18S rRNAs, total RNA that was prepared as described above was run on an Agilent Technologies 2100 Bioanalyzer at the Yale Center for Genome Analysis.

Techniques: Transfection, Nucleic Acid Electrophoresis, Northern Blot, Labeling, One-tailed Test, MANN-WHITNEY

Journal: Biotechnology Research International

Article Title: Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification

doi: 10.4061/2011/838232

Figure Lengend Snippet: Electropherogram and “gel-like” image of positive control on the Agilent Bioanalyzer 2100. The x -axis on the electropherogram represents amplicon size (bp), whist the y -axis represents the measurement response of fluorescence units (FUs). Amplicons of interest in order of size (bp) are lower marker (15), Zein (80), Lectin (90), CaMV (108) and combined CaMV 35s promoter/NOS terminator (131) amplicons, and the upper marker (1500).

Article Snippet: Agilent Bioanalyzer 2100 Agilent Technologies state the Bioanalyzer 2100's advantages to be ease-of-use, speed of analysis, low sample volumes, and high reproducibility [ ].

Techniques: Positive Control, Amplification, Fluorescence, Marker

Journal: PLoS ONE

Article Title: Interferon-?4 (IFNL4) Transcript Expression in Human Liver Tissue Samples

doi: 10.1371/journal.pone.0084026

Figure Lengend Snippet: Electropherograms of IFNL4 amplicons. After completion of PCR amplification, 1 µl of samples was separated and analyzed in the Agilent 2100 bioanalyzer using the DNA 1000 LabChip kit. Electropherograms and gels are shown for two representative samples each of ss469415590 TT homozygotes without any amplification of IFNL4 -specific transcripts ( IFNL4 nd) (#1, #2) or of ΔG homozygotes (#3, #4) and of TT homozygotes (#5, #6) with detectable amounts of hepatic IFNL4 -specific mRNA ( IFNL4 d). Analysis confirmed the lack of products in samples #1 and #2, and the presence of products of the expected size (accuracy ±5 bp, as given by the supplier) both in ΔG and TT homozygotes.

Article Snippet: The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany).

Techniques: Polymerase Chain Reaction, Amplification