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  • 99
    Thermo Fisher nupage transfer buffer
    ApoD and LCAT proteins, but not ApoA1 protein, were present in keratocytes. In a Western blot assay, lysates from keratocytes, dermal fibroblasts and HepG2 cells were solubilized and loaded onto 4 to 12% <t>NuPAGE</t> <t>Bis-Tris</t> gradient gels. After electrophoresis, resolved proteins were transferred onto a nitrocellulose membrane and incubated with antibodies against either ApoA1 ( a ), ApoD ( c ) or LCAT ( e ). Anti-β-Actin served as a loading control ( b , d , f ).
    Nupage Transfer Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2826 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher nupage mes sds running buffer
    Silver-stained <t>SDS-PAGE</t> gel (20% gradient <t>NuPAGE</t> system) of the purification fractions from the culture supernatant of P. pastoris KM71H transformants expressing recombinant hydrophobin RodA ( A. fumigatus ].
    Nupage Mes Sds Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nupage mops sds running buffer
    CHD5 protein expression in NBLS cells following transient transfection with miRNA mimics A. Western blot analysis of transfected cells with indicated microRNAs. Post transfection, cells were washed twice with PBS and isolated cell extracts as described in methods [ 41 ]. Whole cell extracts (100 μg) either transfected with indicated miRNAs or mock transfected were subjected to polyacrylamide gel electrophoresis (4-12% <t>SDS-PAGE),</t> using NuPAGE Bis-Tris gels with <t>MOPS-SDS</t> Running Buffer Allstars siRNA and miRNA-454 were used as negative controls. Proteins were transferred on to nitrocellulose membranes (GE Healthcare Life Sciences) and probed with antibodies using rabbit polyclonal CHD5 , actin (Santa Cruz Biotechnology, CA 1:1000), rabbit polyclonal CHD4 (Bethyl 1:2000), and MYCN monoclonal (1:5000; BD Biosciences). Almost complete reduction of CHD5 protein levels were observed for miR-20b, miR-93, miR-17, and miR-211 as indicated, but no change in CHD4, actin or MYCN levels were seen. B. Densitometric analysis of CHD5 protein expression in NBLS cell line. The number of pixels from each band was measured, and a bar graph was created using the Prism to indicate the difference in CHD5 expression upon miRNA transfection. Data are expressed as the standard error mean (SEM). Statistical analysis was performed using the Prism one way ANOVA method followed by Tukey's post-test. Statistical significance relative to the control Allstar siRNA is indicated: *p
    Nupage Mops Sds Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc phosphate buffered saline pbs
    <t>Metformin</t> inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or <t>PBS</t> by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,
    Phosphate Buffered Saline Pbs, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 2297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher pierce 20x tbs tween 20 buffer
    <t>Metformin</t> inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or <t>PBS</t> by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,
    Pierce 20x Tbs Tween 20 Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Carl Zeiss 20x objective
    FLIP-IT and high resolution (HR)-Light sheet Fluorescence Microscopic (LSFM) reveal the 3D location of P63 + cells in normal human pancreas and chronic pancreatitis FFPE sample. (A) FLIP-IT protocol overview processing of human archival FFPE samples; (B) Overview 3D volume rendering of a large duct system (cyan) with P63 + cells (pink) in normal human pancreas. Objective <t>20x,</t> zoom 0.36. Scale bar corresponds to 100μm; (C) Z-plane clipping of B with KRT19 (cyan), P63 (pink) and Laminin (green). Asterisk indicates reference structure in B. Objective 20x, zoom 0.36. Scale bar corresponds to 100μm; (D) HR-LSFM 3D rendering of a dome positive for P63 (pink) in chronic pancreatitis. Objective 20x, zoom 1. Scale bar corresponds to 25μm. (D’) Inset from D, HR-LSFM 3D volume rendering of D in chronic pancreatitis. Yellow arrows indicate P63 + (pink) cells in contact with the basal membrane (green). White arrows indicate p63 + not in contact with basal membrane. Objective 20x, zoom 1. Scale bar corresponds to 25μm. n≥2
    20x Objective, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Olympus 20x objective
    Oxidative stress in CHED tissue specimens. Tissue sections of CHED corneas (i, ii) and cadaveric corneas (iii, iv) were stained with anti-nitrotyrosine antibody, followed by Alexafluor 488 secondary antibody and were counterstained using DAPI. The sections were imaged under fluorescence microscope using <t>20X</t> objective ( a ) (n = 5). The fold changes in the gene expression of SLC4A11 , NRF2 ( b ) and antioxidant genes HO-1, NQO1, FRT and GR ( c ) were determined from the DM-HCEnC complex obtained from CHED patients during DSEK procedures by quantitative PCR (n = 5). Data points represent individual patients.
    20x Objective, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher sspe
    The <t>FTIR</t> spectrum of <t>SSPE.</t>
    Sspe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 443 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher pierce 20x pbs tween 20 buffer
    The <t>FTIR</t> spectrum of <t>SSPE.</t>
    Pierce 20x Pbs Tween 20 Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher pierce 20x phosphate buffered saline
    Menthol-sensitivity is restricted to Vglut3 lineage DRG neurons. A. Representative confocal images of DRG sections (25 μm) from adult Slc17a8 iCre ;Rosa26 Ai1 mice immunostained with anti-DsRed (TdTomato, red), anti-Neurofilament Heavy (NFH, blue), and anti-β3-Tubulin (green). Images were acquired using a <t>20x,</t> 0.8 NA air objective ( B-C). Baseline normalized, representative traces depicting Fura2-AM ratio (F340/F380) versus time traces of averaged responses from Vglut3 lineage ( B ) and non-Vglut3 lineage ( C ) DRG neurons to various chemosensory stimuli (menthol 100 μM [M, green trace], chloroquine,1 mM [CQ], capsaicin 1 μM [Cap, red trace], and high K+ Ringer’s [K+, black trace]). Colored bar indicates time of agonist application. D . Images of calcium transients in live, dissociated Slc17a8 iCre ;Rosa26 Ai1 DRG neurons quantified in B and C . (Top) Fura2-AM calcium microfluorimetry following menthol application. Green circles indicate menthol-sensitive DRG neurons. (Bottom) Fluorescent image showing TdTomato-expressing (Vglut3 lineage ) DRG neurons. E. Quantification of percentage of Vglut3 lineage (n = 331) and non-Vglut3 lineage (n = 453) neurons responding to individual agonists. F. Quantification of baseline calcium signals between Vglut3 lineage menthol-sensitive neurons and menthol-insensitive neurons (both Vglut3 lineage and non-Vglut3 lineage ). Significance was determined using an unpaired Student’s t test; *P
    Pierce 20x Phosphate Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce 20x phosphate buffered saline/product/Thermo Fisher
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    99
    Thermo Fisher nativepage running buffer
    Menthol-sensitivity is restricted to Vglut3 lineage DRG neurons. A. Representative confocal images of DRG sections (25 μm) from adult Slc17a8 iCre ;Rosa26 Ai1 mice immunostained with anti-DsRed (TdTomato, red), anti-Neurofilament Heavy (NFH, blue), and anti-β3-Tubulin (green). Images were acquired using a <t>20x,</t> 0.8 NA air objective ( B-C). Baseline normalized, representative traces depicting Fura2-AM ratio (F340/F380) versus time traces of averaged responses from Vglut3 lineage ( B ) and non-Vglut3 lineage ( C ) DRG neurons to various chemosensory stimuli (menthol 100 μM [M, green trace], chloroquine,1 mM [CQ], capsaicin 1 μM [Cap, red trace], and high K+ Ringer’s [K+, black trace]). Colored bar indicates time of agonist application. D . Images of calcium transients in live, dissociated Slc17a8 iCre ;Rosa26 Ai1 DRG neurons quantified in B and C . (Top) Fura2-AM calcium microfluorimetry following menthol application. Green circles indicate menthol-sensitive DRG neurons. (Bottom) Fluorescent image showing TdTomato-expressing (Vglut3 lineage ) DRG neurons. E. Quantification of percentage of Vglut3 lineage (n = 331) and non-Vglut3 lineage (n = 453) neurons responding to individual agonists. F. Quantification of baseline calcium signals between Vglut3 lineage menthol-sensitive neurons and menthol-insensitive neurons (both Vglut3 lineage and non-Vglut3 lineage ). Significance was determined using an unpaired Student’s t test; *P
    Nativepage Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher nupage tris acetate sds running buffer
    <t>SDS-PAGE</t> of 2G12-IgM. Purified IgM-012, IgM-012_GL and IgM-617 (control) were separated on 3–12% gradient <t>NuPage®</t> <t>Bis-Tris</t> gels and detected by silver staining.
    Nupage Tris Acetate Sds Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher ssc
    <t>SDS-PAGE</t> of 2G12-IgM. Purified IgM-012, IgM-012_GL and IgM-617 (control) were separated on 3–12% gradient <t>NuPage®</t> <t>Bis-Tris</t> gels and detected by silver staining.
    Ssc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ApoD and LCAT proteins, but not ApoA1 protein, were present in keratocytes. In a Western blot assay, lysates from keratocytes, dermal fibroblasts and HepG2 cells were solubilized and loaded onto 4 to 12% NuPAGE Bis-Tris gradient gels. After electrophoresis, resolved proteins were transferred onto a nitrocellulose membrane and incubated with antibodies against either ApoA1 ( a ), ApoD ( c ) or LCAT ( e ). Anti-β-Actin served as a loading control ( b , d , f ).

    Journal: Biomolecules

    Article Title: LCAT, ApoD, and ApoA1 Expression and Review of Cholesterol Deposition in the Cornea

    doi: 10.3390/biom9120785

    Figure Lengend Snippet: ApoD and LCAT proteins, but not ApoA1 protein, were present in keratocytes. In a Western blot assay, lysates from keratocytes, dermal fibroblasts and HepG2 cells were solubilized and loaded onto 4 to 12% NuPAGE Bis-Tris gradient gels. After electrophoresis, resolved proteins were transferred onto a nitrocellulose membrane and incubated with antibodies against either ApoA1 ( a ), ApoD ( c ) or LCAT ( e ). Anti-β-Actin served as a loading control ( b , d , f ).

    Article Snippet: The gels were then transferred at 4 °C to nitrocellulose membranes using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad Laboratories #1703930) in NuPAGE Transfer Buffer (25 mM Bicine, 25 mM Bis-tris, 1 mM ethylenediaminetetraacetic acid, 0.05 mM chlorobutanol, 20% methanol, pH 7.2) (Invitrogen # NP0006) with a PowerPack Basic Power Supply.

    Techniques: Western Blot, Electrophoresis, Incubation

    Silver-stained SDS-PAGE gel (20% gradient NuPAGE system) of the purification fractions from the culture supernatant of P. pastoris KM71H transformants expressing recombinant hydrophobin RodA ( A. fumigatus ].

    Journal: Microorganisms

    Article Title: Pichia pastoris is a Suitable Host for the Heterologous Expression of Predicted Class I and Class II Hydrophobins for Discovery, Study, and Application in Biotechnology

    doi: 10.3390/microorganisms6010003

    Figure Lengend Snippet: Silver-stained SDS-PAGE gel (20% gradient NuPAGE system) of the purification fractions from the culture supernatant of P. pastoris KM71H transformants expressing recombinant hydrophobin RodA ( A. fumigatus ].

    Article Snippet: Furthermore, the accompanying NuPAGE MES SDS running buffer (Life Technologies) was used.

    Techniques: Staining, SDS Page, Purification, Expressing, Recombinant

    CHD5 protein expression in NBLS cells following transient transfection with miRNA mimics A. Western blot analysis of transfected cells with indicated microRNAs. Post transfection, cells were washed twice with PBS and isolated cell extracts as described in methods [ 41 ]. Whole cell extracts (100 μg) either transfected with indicated miRNAs or mock transfected were subjected to polyacrylamide gel electrophoresis (4-12% SDS-PAGE), using NuPAGE Bis-Tris gels with MOPS-SDS Running Buffer Allstars siRNA and miRNA-454 were used as negative controls. Proteins were transferred on to nitrocellulose membranes (GE Healthcare Life Sciences) and probed with antibodies using rabbit polyclonal CHD5 , actin (Santa Cruz Biotechnology, CA 1:1000), rabbit polyclonal CHD4 (Bethyl 1:2000), and MYCN monoclonal (1:5000; BD Biosciences). Almost complete reduction of CHD5 protein levels were observed for miR-20b, miR-93, miR-17, and miR-211 as indicated, but no change in CHD4, actin or MYCN levels were seen. B. Densitometric analysis of CHD5 protein expression in NBLS cell line. The number of pixels from each band was measured, and a bar graph was created using the Prism to indicate the difference in CHD5 expression upon miRNA transfection. Data are expressed as the standard error mean (SEM). Statistical analysis was performed using the Prism one way ANOVA method followed by Tukey's post-test. Statistical significance relative to the control Allstar siRNA is indicated: *p

    Journal: Oncotarget

    Article Title: Role of microRNAs in epigenetic silencing of the CHD5 tumor suppressor gene in neuroblastomas

    doi: 10.18632/oncotarget.7434

    Figure Lengend Snippet: CHD5 protein expression in NBLS cells following transient transfection with miRNA mimics A. Western blot analysis of transfected cells with indicated microRNAs. Post transfection, cells were washed twice with PBS and isolated cell extracts as described in methods [ 41 ]. Whole cell extracts (100 μg) either transfected with indicated miRNAs or mock transfected were subjected to polyacrylamide gel electrophoresis (4-12% SDS-PAGE), using NuPAGE Bis-Tris gels with MOPS-SDS Running Buffer Allstars siRNA and miRNA-454 were used as negative controls. Proteins were transferred on to nitrocellulose membranes (GE Healthcare Life Sciences) and probed with antibodies using rabbit polyclonal CHD5 , actin (Santa Cruz Biotechnology, CA 1:1000), rabbit polyclonal CHD4 (Bethyl 1:2000), and MYCN monoclonal (1:5000; BD Biosciences). Almost complete reduction of CHD5 protein levels were observed for miR-20b, miR-93, miR-17, and miR-211 as indicated, but no change in CHD4, actin or MYCN levels were seen. B. Densitometric analysis of CHD5 protein expression in NBLS cell line. The number of pixels from each band was measured, and a bar graph was created using the Prism to indicate the difference in CHD5 expression upon miRNA transfection. Data are expressed as the standard error mean (SEM). Statistical analysis was performed using the Prism one way ANOVA method followed by Tukey's post-test. Statistical significance relative to the control Allstar siRNA is indicated: *p

    Article Snippet: Western analysis Whole cell extracts (100 μg), either transfected with indicated miRNAs or mock transfected, were subjected to polyacrylamide gel electrophoresis (4-12% SDS-PAGE), using NuPAGE Bis-Tris gels with MOPS-SDS Running Buffer (Invitrogen, Grand Island, NY).

    Techniques: Expressing, Transfection, Western Blot, Isolation, Polyacrylamide Gel Electrophoresis, SDS Page

    Metformin inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or PBS by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Metformin induces autophagy and G0/G1 phase cell cycle arrest in myeloma by targeting the AMPK/mTORC1 and mTORC2 pathways

    doi: 10.1186/s13046-018-0731-5

    Figure Lengend Snippet: Metformin inhibits tumor growth in an RPMI8266 xenograft mouse model. a NOD/SCID mice received metformin or PBS by oral gavage every day after subcutaneous implantation of RPMI8226 cells. Tumor sizes were monitored every 3 days. b Body weight of mice was monitored every 3 days. c Immunohistochemical analysis of tumors from the metformin and control groups showing upregulation of AMPK and downregulation of mTOR and Ki-67. Expression levels were scored semi- quantitatively as the percentage of positive cells according to the following system: +,

    Article Snippet: Reagents Metformin (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in phosphate-buffered saline (PBS) as a stock solution of 1 M. Primary antibodies for specific detection of p-AMPK (Thr172), AMPK, Phospho-Tuberin/TSC2 (Ser1387), p-mTOR (Ser2481), p-mTOR (Ser2448), mTOR, p-p70S6K (Thr389), p70S6K, p-4EBP1 (Thr37/46), 4EBP1, p-AKT (Ser473), and AKT, as well as horseradish peroxidase (HRP)-conjugated anti-rabbit secondary detection antibodies, were purchased from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: Mouse Assay, Immunohistochemistry, Expressing

    FLIP-IT and high resolution (HR)-Light sheet Fluorescence Microscopic (LSFM) reveal the 3D location of P63 + cells in normal human pancreas and chronic pancreatitis FFPE sample. (A) FLIP-IT protocol overview processing of human archival FFPE samples; (B) Overview 3D volume rendering of a large duct system (cyan) with P63 + cells (pink) in normal human pancreas. Objective 20x, zoom 0.36. Scale bar corresponds to 100μm; (C) Z-plane clipping of B with KRT19 (cyan), P63 (pink) and Laminin (green). Asterisk indicates reference structure in B. Objective 20x, zoom 0.36. Scale bar corresponds to 100μm; (D) HR-LSFM 3D rendering of a dome positive for P63 (pink) in chronic pancreatitis. Objective 20x, zoom 1. Scale bar corresponds to 25μm. (D’) Inset from D, HR-LSFM 3D volume rendering of D in chronic pancreatitis. Yellow arrows indicate P63 + (pink) cells in contact with the basal membrane (green). White arrows indicate p63 + not in contact with basal membrane. Objective 20x, zoom 1. Scale bar corresponds to 25μm. n≥2

    Journal: bioRxiv

    Article Title: Discovery and 3D imaging of a novel ΔNp63-expressing basal cell type in human pancreatic ducts with implications in disease

    doi: 10.1101/2020.08.20.259317

    Figure Lengend Snippet: FLIP-IT and high resolution (HR)-Light sheet Fluorescence Microscopic (LSFM) reveal the 3D location of P63 + cells in normal human pancreas and chronic pancreatitis FFPE sample. (A) FLIP-IT protocol overview processing of human archival FFPE samples; (B) Overview 3D volume rendering of a large duct system (cyan) with P63 + cells (pink) in normal human pancreas. Objective 20x, zoom 0.36. Scale bar corresponds to 100μm; (C) Z-plane clipping of B with KRT19 (cyan), P63 (pink) and Laminin (green). Asterisk indicates reference structure in B. Objective 20x, zoom 0.36. Scale bar corresponds to 100μm; (D) HR-LSFM 3D rendering of a dome positive for P63 (pink) in chronic pancreatitis. Objective 20x, zoom 1. Scale bar corresponds to 25μm. (D’) Inset from D, HR-LSFM 3D volume rendering of D in chronic pancreatitis. Yellow arrows indicate P63 + (pink) cells in contact with the basal membrane (green). White arrows indicate p63 + not in contact with basal membrane. Objective 20x, zoom 1. Scale bar corresponds to 25μm. n≥2

    Article Snippet: High magnification images were acquired using 20x objective with zoom between 1 and 2,5 (magnification 20x and 50x)-16bit.

    Techniques: Fluorescence, Formalin-fixed Paraffin-Embedded

    3D imaging of KRT5-positive basal cells in normal pancreas and expansion as dome-like structures in chronic pancreatitis. (A) Overview 3D rendering of normal human pancreas and (B) chronic pancreatitis from FFPE blocks stained for KRT5 (pink) and KRT7 (cyan). Arrows indicate magnified regions in (C) and (D) respectively for normal pancreas and chronic pancreatitis. Objective 20x, zoom 0.36. Scale bar corresponds to 500μm; (C) HR-LSFM of a KRT5 + (pink) dome on a large KRT7 + (cyan) duct with lumen diameter 120μm at widest point in normal pancreas. Asterisk indicates lumen. Objective 20x, zoom 2.5. Scale bar corresponds to 20μm; (D) HR-LSFM of dome wall showing its constitution in chronic pancreatitis. Objective 20x, zoom 2.5. Scale bar corresponds to 20μm; (C’) inset from C showing in great detail cellular structure of KRT5 + (pink) cells in normal pancreas. Objective 20x, zoom 2.5. Scale bar corresponds to 5μm; (D’) HR-LSFM of dome wall showing flat KRT5 + (pink) and KRT7 + (cyan) cells intercalated (yellow arrow) and KRT5 + (pink) cells lining the exterior of the dome wall. Asterisk indicates lumen. Objective 20x, zoom 2.5. Scale bar corresponds to 5μm. n≥2

    Journal: bioRxiv

    Article Title: Discovery and 3D imaging of a novel ΔNp63-expressing basal cell type in human pancreatic ducts with implications in disease

    doi: 10.1101/2020.08.20.259317

    Figure Lengend Snippet: 3D imaging of KRT5-positive basal cells in normal pancreas and expansion as dome-like structures in chronic pancreatitis. (A) Overview 3D rendering of normal human pancreas and (B) chronic pancreatitis from FFPE blocks stained for KRT5 (pink) and KRT7 (cyan). Arrows indicate magnified regions in (C) and (D) respectively for normal pancreas and chronic pancreatitis. Objective 20x, zoom 0.36. Scale bar corresponds to 500μm; (C) HR-LSFM of a KRT5 + (pink) dome on a large KRT7 + (cyan) duct with lumen diameter 120μm at widest point in normal pancreas. Asterisk indicates lumen. Objective 20x, zoom 2.5. Scale bar corresponds to 20μm; (D) HR-LSFM of dome wall showing its constitution in chronic pancreatitis. Objective 20x, zoom 2.5. Scale bar corresponds to 20μm; (C’) inset from C showing in great detail cellular structure of KRT5 + (pink) cells in normal pancreas. Objective 20x, zoom 2.5. Scale bar corresponds to 5μm; (D’) HR-LSFM of dome wall showing flat KRT5 + (pink) and KRT7 + (cyan) cells intercalated (yellow arrow) and KRT5 + (pink) cells lining the exterior of the dome wall. Asterisk indicates lumen. Objective 20x, zoom 2.5. Scale bar corresponds to 5μm. n≥2

    Article Snippet: High magnification images were acquired using 20x objective with zoom between 1 and 2,5 (magnification 20x and 50x)-16bit.

    Techniques: Imaging, Formalin-fixed Paraffin-Embedded, Staining

    Morphometric analysis of VIP cells (A) Magnified views of the somatodendritic and axonal processes representing the following morphologies; i – horizontal bi-polar, ii – bi-tufted, iii – multipolar and iv – unipolar architectures. Scale bar are 50μm (B) Schematic of the MEC with neurolucida reconstructions depicting the three predominant morphologies observed in the data; horizontal bi-polar in L4 (a; axons in orange, dendrites in green), multipolar VIP neuron in L3 (b; axons in dark blue and dendrites in light blue) and vertical bi-polar morphology seen in L2 (c; axons in red, dendrites in blue). Scale bars are 100μm. For all cells the soma is coloured in orange. (C) Plots depicting siginificant different features in the vertical (i) and horizontal (ii) spread of the dendritic process Each bar represents a mean and SEM (D) Graphs assessing the total length of process contained in a specific layer for (i) axons and (ii) dendrites Each bar represents a mean and SEM (E) Maximum projection intensity of 207 z-stacks of 0.5 μm depth taken in 20X magnification of a bi-tufted neuron recorded in LII of the MEC filled with neurobiotin (GFP). Scale bar is 250μm (F) Morphological features of dendritic (i) and axonal (ii) arbors obtained from sholl analysis. Each data point represents a mean and SEM. LI-III = 8 cells, LIV-VI = 6 cells; *** p

    Journal: bioRxiv

    Article Title: A characterization of the electrophysiological, morphological and input domains of vasoactive intestinal peptide (VIP) interneurons in the medial entorhinal cortex (MEC)

    doi: 10.1101/2020.05.15.097972

    Figure Lengend Snippet: Morphometric analysis of VIP cells (A) Magnified views of the somatodendritic and axonal processes representing the following morphologies; i – horizontal bi-polar, ii – bi-tufted, iii – multipolar and iv – unipolar architectures. Scale bar are 50μm (B) Schematic of the MEC with neurolucida reconstructions depicting the three predominant morphologies observed in the data; horizontal bi-polar in L4 (a; axons in orange, dendrites in green), multipolar VIP neuron in L3 (b; axons in dark blue and dendrites in light blue) and vertical bi-polar morphology seen in L2 (c; axons in red, dendrites in blue). Scale bars are 100μm. For all cells the soma is coloured in orange. (C) Plots depicting siginificant different features in the vertical (i) and horizontal (ii) spread of the dendritic process Each bar represents a mean and SEM (D) Graphs assessing the total length of process contained in a specific layer for (i) axons and (ii) dendrites Each bar represents a mean and SEM (E) Maximum projection intensity of 207 z-stacks of 0.5 μm depth taken in 20X magnification of a bi-tufted neuron recorded in LII of the MEC filled with neurobiotin (GFP). Scale bar is 250μm (F) Morphological features of dendritic (i) and axonal (ii) arbors obtained from sholl analysis. Each data point represents a mean and SEM. LI-III = 8 cells, LIV-VI = 6 cells; *** p

    Article Snippet: Biocytin-labeled VIP cells were imaged under the MBF Zeiss ApoTome microscope with a 20X magnification (Carl Zeiss, Germany).

    Techniques:

    Monosynaptic retrograde tracing in VIP interneurons using rabies viral strategy (A) Schematic of the tracing strategy. Starter cells or cell bodies infected by the helper and EnvA (RV-eGFP) protein are labelled in red and green respectively. (B) Image of the injection site and starter cells from one animal in 10X magnification. Layers of the MEC are marked by dashed lines.A 20X maginification of ramed area is seen in (i) where the starter and input cells within the MEC are observed and denoted by white and yellow arrows respectively. Transfection of VIP cell by TVA receptors (ii) and (iii) EnvA protein. Coloclaization of the two channels is seen (iv) in the starter cell marked by white arrows where as presenence of TVA is absent in the other cell. 40X Maginifcation of a starter cell as seen from a second animal (v-vii). Presence of colocalization of TVA receptors (v) and EnvA protein (vi) is seen in vii. (C) Monosynaptic inputs to MEC VIP interneurons from the Me5 nuclei (ii) and the SPO (iii) as seen in the coronal plane (D) Sagittal view of the mid-brain and brainstem. (i)Inputs from the Me5 and the SPO are labelled in green. The LC is labelled in red using a stain for tyrosine hydroxylase (TH). 20X magnification of Me5 tract (ii) and overlay of atlas plane to confirm input from (iii)SPO (E) Serial reconstruction of representative sections of Me5 input to. Matching mouse brain atlas planes are aligned with sections. Insets represent a magnified view of the corresponding sections. Me5 – Mesencephalic trigeminal nucleus, SPO – superior paraolivary nucleus, LC – Locus coeruleus.

    Journal: bioRxiv

    Article Title: A characterization of the electrophysiological, morphological and input domains of vasoactive intestinal peptide (VIP) interneurons in the medial entorhinal cortex (MEC)

    doi: 10.1101/2020.05.15.097972

    Figure Lengend Snippet: Monosynaptic retrograde tracing in VIP interneurons using rabies viral strategy (A) Schematic of the tracing strategy. Starter cells or cell bodies infected by the helper and EnvA (RV-eGFP) protein are labelled in red and green respectively. (B) Image of the injection site and starter cells from one animal in 10X magnification. Layers of the MEC are marked by dashed lines.A 20X maginification of ramed area is seen in (i) where the starter and input cells within the MEC are observed and denoted by white and yellow arrows respectively. Transfection of VIP cell by TVA receptors (ii) and (iii) EnvA protein. Coloclaization of the two channels is seen (iv) in the starter cell marked by white arrows where as presenence of TVA is absent in the other cell. 40X Maginifcation of a starter cell as seen from a second animal (v-vii). Presence of colocalization of TVA receptors (v) and EnvA protein (vi) is seen in vii. (C) Monosynaptic inputs to MEC VIP interneurons from the Me5 nuclei (ii) and the SPO (iii) as seen in the coronal plane (D) Sagittal view of the mid-brain and brainstem. (i)Inputs from the Me5 and the SPO are labelled in green. The LC is labelled in red using a stain for tyrosine hydroxylase (TH). 20X magnification of Me5 tract (ii) and overlay of atlas plane to confirm input from (iii)SPO (E) Serial reconstruction of representative sections of Me5 input to. Matching mouse brain atlas planes are aligned with sections. Insets represent a magnified view of the corresponding sections. Me5 – Mesencephalic trigeminal nucleus, SPO – superior paraolivary nucleus, LC – Locus coeruleus.

    Article Snippet: Biocytin-labeled VIP cells were imaged under the MBF Zeiss ApoTome microscope with a 20X magnification (Carl Zeiss, Germany).

    Techniques: Retrograde Tracing, Infection, Injection, Transfection, Staining

    Specificity of cre-driven fluorescence for VIP in VIPcre/tdTom mice and presence of colocalization with other molecular markers. (A) Co-expression of VIP (green) and tdtom (red) in a sagittal section of the MEC at low maginification 10X. Layers of the MEC (white dashed lines) marked using DAPI as reference. High maginification of framed area (orange box) is shown in B-B”. (B-K”) 20X maximum projection intensities of a 40-μm thick section of superficial MEC treated with IHC using αVIP AB (B-B”), αGABA AB (E-E”) and αCR AB (H-H”). Fluorescence of cre-driven tdTomato expression is shown in red (B’-H’) and colocalization of the two channels is shown in (B”-H”). Presence of colocalization is indicated by yellow cell bodies and white arrow heads. Graphs depicting the total number of cells present in the MEC (C, F and I) and distribution of somas in different layers of the MEC (D, G and J) for αVIP, αGABA and αCR respectively are shown here. Each bar represents a mean and error bars represent SEM. For panels B-H” scale bars are 250μm. Scale bar in A is 500μm.

    Journal: bioRxiv

    Article Title: A characterization of the electrophysiological, morphological and input domains of vasoactive intestinal peptide (VIP) interneurons in the medial entorhinal cortex (MEC)

    doi: 10.1101/2020.05.15.097972

    Figure Lengend Snippet: Specificity of cre-driven fluorescence for VIP in VIPcre/tdTom mice and presence of colocalization with other molecular markers. (A) Co-expression of VIP (green) and tdtom (red) in a sagittal section of the MEC at low maginification 10X. Layers of the MEC (white dashed lines) marked using DAPI as reference. High maginification of framed area (orange box) is shown in B-B”. (B-K”) 20X maximum projection intensities of a 40-μm thick section of superficial MEC treated with IHC using αVIP AB (B-B”), αGABA AB (E-E”) and αCR AB (H-H”). Fluorescence of cre-driven tdTomato expression is shown in red (B’-H’) and colocalization of the two channels is shown in (B”-H”). Presence of colocalization is indicated by yellow cell bodies and white arrow heads. Graphs depicting the total number of cells present in the MEC (C, F and I) and distribution of somas in different layers of the MEC (D, G and J) for αVIP, αGABA and αCR respectively are shown here. Each bar represents a mean and error bars represent SEM. For panels B-H” scale bars are 250μm. Scale bar in A is 500μm.

    Article Snippet: Biocytin-labeled VIP cells were imaged under the MBF Zeiss ApoTome microscope with a 20X magnification (Carl Zeiss, Germany).

    Techniques: Fluorescence, Mouse Assay, Expressing, Immunohistochemistry

    Oxidative stress in CHED tissue specimens. Tissue sections of CHED corneas (i, ii) and cadaveric corneas (iii, iv) were stained with anti-nitrotyrosine antibody, followed by Alexafluor 488 secondary antibody and were counterstained using DAPI. The sections were imaged under fluorescence microscope using 20X objective ( a ) (n = 5). The fold changes in the gene expression of SLC4A11 , NRF2 ( b ) and antioxidant genes HO-1, NQO1, FRT and GR ( c ) were determined from the DM-HCEnC complex obtained from CHED patients during DSEK procedures by quantitative PCR (n = 5). Data points represent individual patients.

    Journal: Scientific Reports

    Article Title: SLC4A11 depletion impairs NRF2 mediated antioxidant signaling and increases reactive oxygen species in human corneal endothelial cells during oxidative stress

    doi: 10.1038/s41598-017-03654-4

    Figure Lengend Snippet: Oxidative stress in CHED tissue specimens. Tissue sections of CHED corneas (i, ii) and cadaveric corneas (iii, iv) were stained with anti-nitrotyrosine antibody, followed by Alexafluor 488 secondary antibody and were counterstained using DAPI. The sections were imaged under fluorescence microscope using 20X objective ( a ) (n = 5). The fold changes in the gene expression of SLC4A11 , NRF2 ( b ) and antioxidant genes HO-1, NQO1, FRT and GR ( c ) were determined from the DM-HCEnC complex obtained from CHED patients during DSEK procedures by quantitative PCR (n = 5). Data points represent individual patients.

    Article Snippet: The sections were counterstained with DAPI (Abcam, Cambridge) and observed under fluorescent microscope (Olympus IX73) using 20X objective and imaged using Olympus DP71 camera.

    Techniques: Staining, Fluorescence, Microscopy, Expressing, Real-time Polymerase Chain Reaction

    Overexpression of SLC4A11 in HEK 293 cells. Cells were transfected with wild-type. SLC4A11 plasmid or empty vectors and exposed to 250 µM of tBH. Cell viability ( a ), ROS generation ( b ) and Nitrotyrosine staining ( c ) were determined. For nitrotyrosine staining, cells were stained using anti-nitrotyrosine antibody, followed by Alexafluor 488 secondary antibody. Cells were imaged both under bright field and fluorescence and images were captured by Olympus IX73 using 20X objective. The experiments a, and b, were done three times in duplicates and experiment c was performed two times.

    Journal: Scientific Reports

    Article Title: SLC4A11 depletion impairs NRF2 mediated antioxidant signaling and increases reactive oxygen species in human corneal endothelial cells during oxidative stress

    doi: 10.1038/s41598-017-03654-4

    Figure Lengend Snippet: Overexpression of SLC4A11 in HEK 293 cells. Cells were transfected with wild-type. SLC4A11 plasmid or empty vectors and exposed to 250 µM of tBH. Cell viability ( a ), ROS generation ( b ) and Nitrotyrosine staining ( c ) were determined. For nitrotyrosine staining, cells were stained using anti-nitrotyrosine antibody, followed by Alexafluor 488 secondary antibody. Cells were imaged both under bright field and fluorescence and images were captured by Olympus IX73 using 20X objective. The experiments a, and b, were done three times in duplicates and experiment c was performed two times.

    Article Snippet: The sections were counterstained with DAPI (Abcam, Cambridge) and observed under fluorescent microscope (Olympus IX73) using 20X objective and imaged using Olympus DP71 camera.

    Techniques: Over Expression, Transfection, Plasmid Preparation, Staining, Fluorescence

    Representative cases of elastin fragmentation in the media. Movat images at 20X magnification: (A) Neck region with low elastin fragmentation (score 1); (B) LA3 region with moderate elastin fragmentation (score 2); (C) RA1 region with high elastin fragmentation (score 3) and (D) RA5 region with no elastin content (score 4). Scale bar 0.1 mm.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Case Study: Intra-Patient Heterogeneity of Aneurysmal Tissue Properties

    doi: 10.3389/fcvm.2018.00082

    Figure Lengend Snippet: Representative cases of elastin fragmentation in the media. Movat images at 20X magnification: (A) Neck region with low elastin fragmentation (score 1); (B) LA3 region with moderate elastin fragmentation (score 2); (C) RA1 region with high elastin fragmentation (score 3) and (D) RA5 region with no elastin content (score 4). Scale bar 0.1 mm.

    Article Snippet: Figures , show Movat's stain at 20X magnification for four regions to demonstrate the degree of elastin fragmentation in the media.

    Techniques:

    The FTIR spectrum of SSPE.

    Journal: International Journal of Environmental Research and Public Health

    Article Title: Adsorption Thermodynamics and Dynamics of Three Typical Dyes onto Bio-adsorbent Spent Substrate of Pleurotus eryngii

    doi: 10.3390/ijerph16050679

    Figure Lengend Snippet: The FTIR spectrum of SSPE.

    Article Snippet: Assays by Fourier Transform Infrared Spectroscopy (FTIR) KBr pellets were prepared with 2 mg SSPE in 40 mg KBr for FTIR spectroscopy using a Nicolet iS50 spectrometer (Thermo Scientific, Waltham, MA, USA).

    Techniques:

    Menthol-sensitivity is restricted to Vglut3 lineage DRG neurons. A. Representative confocal images of DRG sections (25 μm) from adult Slc17a8 iCre ;Rosa26 Ai1 mice immunostained with anti-DsRed (TdTomato, red), anti-Neurofilament Heavy (NFH, blue), and anti-β3-Tubulin (green). Images were acquired using a 20x, 0.8 NA air objective ( B-C). Baseline normalized, representative traces depicting Fura2-AM ratio (F340/F380) versus time traces of averaged responses from Vglut3 lineage ( B ) and non-Vglut3 lineage ( C ) DRG neurons to various chemosensory stimuli (menthol 100 μM [M, green trace], chloroquine,1 mM [CQ], capsaicin 1 μM [Cap, red trace], and high K+ Ringer’s [K+, black trace]). Colored bar indicates time of agonist application. D . Images of calcium transients in live, dissociated Slc17a8 iCre ;Rosa26 Ai1 DRG neurons quantified in B and C . (Top) Fura2-AM calcium microfluorimetry following menthol application. Green circles indicate menthol-sensitive DRG neurons. (Bottom) Fluorescent image showing TdTomato-expressing (Vglut3 lineage ) DRG neurons. E. Quantification of percentage of Vglut3 lineage (n = 331) and non-Vglut3 lineage (n = 453) neurons responding to individual agonists. F. Quantification of baseline calcium signals between Vglut3 lineage menthol-sensitive neurons and menthol-insensitive neurons (both Vglut3 lineage and non-Vglut3 lineage ). Significance was determined using an unpaired Student’s t test; *P

    Journal: bioRxiv

    Article Title: Tetrodotoxin-sensitive sodium channels mediate action potential firing and excitability in menthol-sensitive Vglut3-lineage sensory neurons

    doi: 10.1101/670620

    Figure Lengend Snippet: Menthol-sensitivity is restricted to Vglut3 lineage DRG neurons. A. Representative confocal images of DRG sections (25 μm) from adult Slc17a8 iCre ;Rosa26 Ai1 mice immunostained with anti-DsRed (TdTomato, red), anti-Neurofilament Heavy (NFH, blue), and anti-β3-Tubulin (green). Images were acquired using a 20x, 0.8 NA air objective ( B-C). Baseline normalized, representative traces depicting Fura2-AM ratio (F340/F380) versus time traces of averaged responses from Vglut3 lineage ( B ) and non-Vglut3 lineage ( C ) DRG neurons to various chemosensory stimuli (menthol 100 μM [M, green trace], chloroquine,1 mM [CQ], capsaicin 1 μM [Cap, red trace], and high K+ Ringer’s [K+, black trace]). Colored bar indicates time of agonist application. D . Images of calcium transients in live, dissociated Slc17a8 iCre ;Rosa26 Ai1 DRG neurons quantified in B and C . (Top) Fura2-AM calcium microfluorimetry following menthol application. Green circles indicate menthol-sensitive DRG neurons. (Bottom) Fluorescent image showing TdTomato-expressing (Vglut3 lineage ) DRG neurons. E. Quantification of percentage of Vglut3 lineage (n = 331) and non-Vglut3 lineage (n = 453) neurons responding to individual agonists. F. Quantification of baseline calcium signals between Vglut3 lineage menthol-sensitive neurons and menthol-insensitive neurons (both Vglut3 lineage and non-Vglut3 lineage ). Significance was determined using an unpaired Student’s t test; *P

    Article Snippet: Cells were incubated at 37°C in 5% CO2 with the transfection solution for 3 h, followed by two washes with sterile phosphate buffered saline and addition of new cell culture media.

    Techniques: Mouse Assay, Expressing

    SDS-PAGE of 2G12-IgM. Purified IgM-012, IgM-012_GL and IgM-617 (control) were separated on 3–12% gradient NuPage® Bis-Tris gels and detected by silver staining.

    Journal: Biochimica et Biophysica Acta

    Article Title: Introduction of germline residues improves the stability of anti-HIV mAb 2G12-IgM

    doi: 10.1016/j.bbapap.2015.02.018

    Figure Lengend Snippet: SDS-PAGE of 2G12-IgM. Purified IgM-012, IgM-012_GL and IgM-617 (control) were separated on 3–12% gradient NuPage® Bis-Tris gels and detected by silver staining.

    Article Snippet: Purified protein samples were loaded onto NuPage® gradient 3–12% Bis–Tris gels (Life Technologies, # BN1001BOX) and run at 200 V for 60 min in Tris-Acetate SDS buffer (Life Technologies, # LA0041).

    Techniques: SDS Page, Purification, Silver Staining

    Oligomerization pattern of primate ficolin-2 and ficolin-3 was evaluated by SDS-PAGE subjected to western blot. Samples were analysed by SDS-PAGE on 3–8% Tris-actetate gels under non-reduced and reduced conditions subjected to western blot and detected with (A) mono- and polyclonal anti-ficolin-2 or (B) mono- and polyclonal anti-ficolin-3. Arrows show the multimers and monomers of the ficolins.

    Journal: PLoS ONE

    Article Title: Allelic Lineages of the Ficolin Genes (FCNs) Are Passed from Ancestral to Descendant Primates

    doi: 10.1371/journal.pone.0028187

    Figure Lengend Snippet: Oligomerization pattern of primate ficolin-2 and ficolin-3 was evaluated by SDS-PAGE subjected to western blot. Samples were analysed by SDS-PAGE on 3–8% Tris-actetate gels under non-reduced and reduced conditions subjected to western blot and detected with (A) mono- and polyclonal anti-ficolin-2 or (B) mono- and polyclonal anti-ficolin-3. Arrows show the multimers and monomers of the ficolins.

    Article Snippet: The samples were loaded on NuPAGE 3–8% Tris–acetate gels with Tris-Acetate SDS running buffer (Invitrogen, Taastrup, Denmark) and subsequently the separated proteins were blotted onto nitrocellulose membranes using the XCell II Mini-Cell blot apparatus, NuPAGE transfer buffer and Hybond ECL nitrocellulose membrane.

    Techniques: SDS Page, Western Blot