Journal: Animals : an Open Access Journal from MDPI

Article Title: Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells

doi: 10.3390/ani11041073

Figure Lengend Snippet: Confocal laser scanning microscope image of cytokeratin protein in prostaglandin F2 alpha (PGF2α)-induced bovine luteal thecal cells (LTCs). The LTCs were incubated in culture media supplemented 0 mM ( a , e ), 0.01 mM ( b , f ), 0.1 mM ( c , g ), and 1.0 mM ( d , h ) PGF2α. Green: cytokeratin protein, blue: nucleus, yellow arrows: damaged cytokeratin, white line: 50 μm, yellow line: 20 μm.

Article Snippet: LTCs were pre-incubated for 24 h, the cells washed twice with PBS containing 0.1% BSA and the medium was replaced with fresh medium containing phenol red free D/F12 (Sigma) supplemented with 0.1% BSA, 1% P/S solution, and treated with 0, 0.01, 0.1 and 1.0 mM PGF2α (sc-203219, SCBT, Santacruz, CA, USA) for 24 h. The minimum concentration of PGF2α to induce regression in bovine LTCs was determined according to previously studies [ , ].

Techniques: Laser-Scanning Microscopy, Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells

doi: 10.3390/ani11041073

Figure Lengend Snippet: Confocal laser scanning microscope image of vimentin protein in prostaglandin F2 alpha (PGF2α)-induced bovine luteal thecal cells (LTCs). The LTCs were incubated in culture media supplemented 0 mM ( a , e ), 0.01 mM ( b , f ), 0.1 mM ( c , g ), and 1.0 mM ( d , h ) PGF2α. Green: vimentin protein, blue: nucleus, white arrows: damaged vimentin, white line: 50 μm, yellow line: 20 μm.

Article Snippet: LTCs were pre-incubated for 24 h, the cells washed twice with PBS containing 0.1% BSA and the medium was replaced with fresh medium containing phenol red free D/F12 (Sigma) supplemented with 0.1% BSA, 1% P/S solution, and treated with 0, 0.01, 0.1 and 1.0 mM PGF2α (sc-203219, SCBT, Santacruz, CA, USA) for 24 h. The minimum concentration of PGF2α to induce regression in bovine LTCs was determined according to previously studies [ , ].

Techniques: Laser-Scanning Microscopy, Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells

doi: 10.3390/ani11041073

Figure Lengend Snippet: Influence of prostaglandin F2 alpha (PGF2α) on Rho and Rho-associated protein kinase (ROCK) protein in bovine luteal theca cells (LTCs). The protein was analyzed using western blotting. * p < 0.05, n = 5.

Article Snippet: LTCs were pre-incubated for 24 h, the cells washed twice with PBS containing 0.1% BSA and the medium was replaced with fresh medium containing phenol red free D/F12 (Sigma) supplemented with 0.1% BSA, 1% P/S solution, and treated with 0, 0.01, 0.1 and 1.0 mM PGF2α (sc-203219, SCBT, Santacruz, CA, USA) for 24 h. The minimum concentration of PGF2α to induce regression in bovine LTCs was determined according to previously studies [ , ].

Techniques: Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells

doi: 10.3390/ani11041073

Figure Lengend Snippet: Expression of desmoplakin in prostaglandin F2 alpha (PGF2α)-induced bovine luteal thecal cells (LTCs). The LTCs were observed using confocal laser scanning microscope. The LTCs were incubated with 0 mM ( a , c ) and 0.01 mM ( b , d ) PGF2α for 24 h, white arrows: normal desmoplakin, yellow arrows: damaged desmoplakin, white line: 50 μm, yellow line: 20 μm.

Article Snippet: LTCs were pre-incubated for 24 h, the cells washed twice with PBS containing 0.1% BSA and the medium was replaced with fresh medium containing phenol red free D/F12 (Sigma) supplemented with 0.1% BSA, 1% P/S solution, and treated with 0, 0.01, 0.1 and 1.0 mM PGF2α (sc-203219, SCBT, Santacruz, CA, USA) for 24 h. The minimum concentration of PGF2α to induce regression in bovine LTCs was determined according to previously studies [ , ].

Techniques: Expressing, Laser-Scanning Microscopy, Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells

doi: 10.3390/ani11041073

Figure Lengend Snippet: Effect of prostaglandin F2 alpha (PGF2α) on cell–cell adhesion ability in bovine luteal theca cells (LTCs). The cell-tracker Red stained LTCs were incubated with 0 mM ( a ) and 0.01 mM ( b ) PGF2α on the monolayer LTCs. The attached LTCs (( a , b ), red dots) on the monolayer cells were counted using the segmentation tools of ImageJ, and the number of attached cells was normalized to 0 M PGF2α treatment ( c ). * p < 0.05, n = 5.

Article Snippet: LTCs were pre-incubated for 24 h, the cells washed twice with PBS containing 0.1% BSA and the medium was replaced with fresh medium containing phenol red free D/F12 (Sigma) supplemented with 0.1% BSA, 1% P/S solution, and treated with 0, 0.01, 0.1 and 1.0 mM PGF2α (sc-203219, SCBT, Santacruz, CA, USA) for 24 h. The minimum concentration of PGF2α to induce regression in bovine LTCs was determined according to previously studies [ , ].

Techniques: Staining, Incubation