Journal:

Article Title: Tumorigenic conversion of immortal human keratinocytes through stromal cell activation

doi:

Figure Lengend Snippet: PDGF-B-transfected cells secrete a mitogenic activity for fibroblasts. Fibroblasts plated at 4 × 103 cells/cm2 (in multiwell plates) were cocultured with control-transfected (HaCaT/neo) or PDGF-B-transfected (clones 17 and 18) HaCaT cells (4 × 104 cells/cm2) (on filter inserts) in supplemented keratinocyte defined medium containing 0.5 mM Ca2+ and 0.5% fetal calf serum. Parallel cell cultures received a neutralizing antibody to PDGF-BB (2 μg/ml). The number of fibroblasts was determined after 5 days. Bars represent the mean of duplicate samples of two independent experiments ± SD.

Article Snippet: Cells were allowed to attach for 12 hr, washed three times with serum-free 4 × MEM and shifted to supplemented keratinocyte defined medium (Promo Cell, Heidelberg) containing 0.5 mM Ca 2+ and 0.5% fetal calf serum.

Techniques: Transfection, Activity Assay, Clone Assay

Journal: bioRxiv

Article Title: The Smallest Intestine (TSI) – a low volume in vitro model of the small intestine with increased throughput

doi: 10.1101/244764

Figure Lengend Snippet: Survival of three probiotic strains during simulated small intestinal passage of the TSI model simulating fed/fasted conditions and with (with) or without (W/O) addition of ileal microbiota: (a) Lactobacillus plantarum LP 80, (b) Lactobacillus rhamnosus GG and (c) Lactobacillus casei Zll. Significant differences in survival of bacteria before and after each experiment were marked. Moreover, significant impact of ileal microbiota on probiotic survival was marked above bars for each experiment. All experiments were performed in triplicates (n = 3)

Article Snippet: Lactobacillus rhamnosus GG (DSM 20021) was purchased from DSMZ, Lactobacillus plantarum (LP80 pCBH1 GZH+) encoding a bile salts hydrolase enzyme (EC 3.5.1.24) was obtained from Ghent University, Belgium.

Techniques: