20 u t4 dna ligase Search Results


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  • 99
    New England Biolabs t4 dna ligase
    Gel-electrophoresis of ligation products. The ligation products were electrophoresed on 8M Urea 10% PAGE at 65 ºC and were visualized with fluorescence of FITC and then visualized after staining by SYBR Green II using an imager. Lane CY: control, Linker-Y. Lane CS: control, mRNA without ligation. Lane CR: control, Linker-S. Lane Y: Y-ligation with T4 RNA ligase. Lane R: splint ligation with T4 RNA ligase. Lane D: splint ligation with <t>T4</t> DNA ligase.
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 49967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher t4 dna ligase
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    T4 Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25057 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    t4 dna ligase - by Bioz Stars, 2020-09
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    94
    Promega t4 dna ligase
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    T4 Dna Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 11518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs taq dna ligase
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1707 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher t4 ligase
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    T4 Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5002 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    t4 ligase - by Bioz Stars, 2020-09
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    99
    Promega rnase inhibitor plus
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    Rnase Inhibitor Plus, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega 20 u t4 dna ligase
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    20 U T4 Dna Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher escherichia coli dna ligase
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    Escherichia Coli Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim t4 dna ligase
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    T4 Dna Ligase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher t4 rna ligase
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    T4 Rna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher t4 polynucleotide kinase 10 u µl
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    T4 Polynucleotide Kinase 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega dna ligase enzymes
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    Dna Ligase Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher klenow fragment 10 u µl
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    Klenow Fragment 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs e coli dna ligase
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    E Coli Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 463 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli dna ligase - by Bioz Stars, 2020-09
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    99
    Thermo Fisher rnase t1
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    Rnase T1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3695 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rnase t1 - by Bioz Stars, 2020-09
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    93
    Thermo Fisher eco105i snabi 10 u µl
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    Eco105i Snabi 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs hmgb protein
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    Hmgb Protein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs taq i restriction endonuclease
    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of <t>T4-DNA</t> ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.
    Taq I Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Gel-electrophoresis of ligation products. The ligation products were electrophoresed on 8M Urea 10% PAGE at 65 ºC and were visualized with fluorescence of FITC and then visualized after staining by SYBR Green II using an imager. Lane CY: control, Linker-Y. Lane CS: control, mRNA without ligation. Lane CR: control, Linker-S. Lane Y: Y-ligation with T4 RNA ligase. Lane R: splint ligation with T4 RNA ligase. Lane D: splint ligation with T4 DNA ligase.

    Journal: Biological Procedures Online

    Article Title: An Efficient Ligation Method in the Making of an in vitro Virus for in vitro Protein Evolution

    doi: 10.1251/bpo33

    Figure Lengend Snippet: Gel-electrophoresis of ligation products. The ligation products were electrophoresed on 8M Urea 10% PAGE at 65 ºC and were visualized with fluorescence of FITC and then visualized after staining by SYBR Green II using an imager. Lane CY: control, Linker-Y. Lane CS: control, mRNA without ligation. Lane CR: control, Linker-S. Lane Y: Y-ligation with T4 RNA ligase. Lane R: splint ligation with T4 RNA ligase. Lane D: splint ligation with T4 DNA ligase.

    Article Snippet: The ligation reaction with T4 DNA ligase (10 U/µl, NEB) was performed according to the supplier's recommendation at 25 ºC.

    Techniques: Nucleic Acid Electrophoresis, Ligation, Polyacrylamide Gel Electrophoresis, Fluorescence, Staining, SYBR Green Assay

    Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of T4-DNA ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.

    Journal: Journal of Bacteriology

    Article Title: Defining the Functionally Important Domain and Amino Acid Residues in Mycobacterium tuberculosis Integration Host Factor for Genome Stability, DNA Binding, and Integrative Recombination

    doi: 10.1128/JB.00357-17

    Figure Lengend Snippet: Wild-type mIHF and mIHF-CTD, but not mIHF-NTD, promote multimerization-circularization of a DNA fragment containing 140 bp. The assay was performed as described in Materials and Methods. The reaction mixtures contained 0.25 nM 32 P-labeled 140-bp DNA in the absence (lanes 1, 2, and 9) or presence (lanes 4 to 8) of 20, 40, 80, 160, and 200 nM wild-type mIHF (A), mIHF-CTD (B), or mIHF-R173 (C) with 20 U of T4-DNA ligase, as specified. Subsequently, the samples in lanes 9 to 12 were incubated with ExoIII. The plus and minus symbols shown on top of the gel represent incubations performed in the presence or absence of Exo III and T4 DNA ligase.

    Article Snippet: After incubation at 37°C for 30 min, 20 U of T4 DNA ligase (Fermentas) and 1× ligase buffer were added, and the incubation was extended for 30 min.

    Techniques: Labeling, Incubation