20 amino acid farnesylation signal Takara Search Results


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    Thermo Fisher ecori
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    Flow cytometry analysis for the change of immune-related surface markers on <t>IFN-γ</t> treated hChonJ. After hChonJ cells were exposed to 300 IU/mL of IFN-γ, the cells were labeled with antibodies against immune-related surface markers, including MHC class I (HLA-ABC), MHC class II (HLA-DR), co-stimulatory molecules (CD80 and CD86), co-inhibitory molecules (PD-L1 and PD-L2) and immunoglobulin G1 isotype control. The expression of MHC class I and II molecules ( a ) was increased, but there was no change in the expression level of the co-stimulatory molecules CD80 and CD86 ( b ). In the case of co-inhibitory molecules, PD-L1 expression was increased and the expression of PD-L2 was detected which was not expressed on IFN-γ untreated hChonJ cells ( c ). These results are the representative of at least 3 independent experiments
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    Flow cytometry analysis for the change of immune-related surface markers on <t>IFN-γ</t> treated hChonJ. After hChonJ cells were exposed to 300 IU/mL of IFN-γ, the cells were labeled with antibodies against immune-related surface markers, including MHC class I (HLA-ABC), MHC class II (HLA-DR), co-stimulatory molecules (CD80 and CD86), co-inhibitory molecules (PD-L1 and PD-L2) and immunoglobulin G1 isotype control. The expression of MHC class I and II molecules ( a ) was increased, but there was no change in the expression level of the co-stimulatory molecules CD80 and CD86 ( b ). In the case of co-inhibitory molecules, PD-L1 expression was increased and the expression of PD-L2 was detected which was not expressed on IFN-γ untreated hChonJ cells ( c ). These results are the representative of at least 3 independent experiments
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    Directed transport of GAP43 by transient piggybacking on exocytic vesicles. (A) Flux analysis of photoactivated protein in processes of transfected PC12 cells. Top, schematic showing the region of photoactivation (gray box) and the position of the recording regions distal and proximal from the center of activation. Bottom, ratios of distal to proximal fluorescence at different times after activation. Mean ± SEM, n = 7–9. The percentage of processes that show higher distal than proximal fluorescence is given in the boxes. *Significantly higher values compared with 1.0. (B) TIRF image of a part of a cell body of a living PC12 cell expressing GAP43-HaloTag (labeled with HTL-OG; green) and <t>synaptophysin-mCherry</t> (red). The focal plane was adjusted in such a way as to visualize a region above the cellular contact site, and the nucleus is visible at the right. Note the presence of green and red structures with vesicular appearance. Note that some structures show yellow fluorescence (arrowheads) indicative of colocalization of GAP43 with vesicular structures. Scale bar, 10 μm. Bottom, time series of TIRF micrographs at the transition of a neurite shaft (n.s.) to the growth cone (g.c.). An example of a particle showing colocalization of GAP43 and synaptophysin, which can be tracked for several seconds, is shown. Scale bar, 10 μm (left), 5 μm (right). The table gives numbers for individual tracking events with colocalization of HTL-OG–labeled GAP43-HaloTag and synaptophysin-mCherry. Note that double-labeled particles move with an average speed of 1.5 ± 0.2 μm/s ( n = 10), similar to the speed of fast axonal transport. Statistical analysis was performed using Student's t test. *, p
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    Directed transport of GAP43 by transient piggybacking on exocytic vesicles. (A) Flux analysis of photoactivated protein in processes of transfected PC12 cells. Top, schematic showing the region of photoactivation (gray box) and the position of the recording regions distal and proximal from the center of activation. Bottom, ratios of distal to proximal fluorescence at different times after activation. Mean ± SEM, n = 7–9. The percentage of processes that show higher distal than proximal fluorescence is given in the boxes. *Significantly higher values compared with 1.0. (B) TIRF image of a part of a cell body of a living PC12 cell expressing GAP43-HaloTag (labeled with HTL-OG; green) and <t>synaptophysin-mCherry</t> (red). The focal plane was adjusted in such a way as to visualize a region above the cellular contact site, and the nucleus is visible at the right. Note the presence of green and red structures with vesicular appearance. Note that some structures show yellow fluorescence (arrowheads) indicative of colocalization of GAP43 with vesicular structures. Scale bar, 10 μm. Bottom, time series of TIRF micrographs at the transition of a neurite shaft (n.s.) to the growth cone (g.c.). An example of a particle showing colocalization of GAP43 and synaptophysin, which can be tracked for several seconds, is shown. Scale bar, 10 μm (left), 5 μm (right). The table gives numbers for individual tracking events with colocalization of HTL-OG–labeled GAP43-HaloTag and synaptophysin-mCherry. Note that double-labeled particles move with an average speed of 1.5 ± 0.2 μm/s ( n = 10), similar to the speed of fast axonal transport. Statistical analysis was performed using Student's t test. *, p
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    Image Search Results


    Flow cytometry analysis for the change of immune-related surface markers on IFN-γ treated hChonJ. After hChonJ cells were exposed to 300 IU/mL of IFN-γ, the cells were labeled with antibodies against immune-related surface markers, including MHC class I (HLA-ABC), MHC class II (HLA-DR), co-stimulatory molecules (CD80 and CD86), co-inhibitory molecules (PD-L1 and PD-L2) and immunoglobulin G1 isotype control. The expression of MHC class I and II molecules ( a ) was increased, but there was no change in the expression level of the co-stimulatory molecules CD80 and CD86 ( b ). In the case of co-inhibitory molecules, PD-L1 expression was increased and the expression of PD-L2 was detected which was not expressed on IFN-γ untreated hChonJ cells ( c ). These results are the representative of at least 3 independent experiments

    Journal: BMC Musculoskeletal Disorders

    Article Title: Immunogenicity and immunomodulatory effects of the human chondrocytes, hChonJ

    doi: 10.1186/s12891-017-1547-8

    Figure Lengend Snippet: Flow cytometry analysis for the change of immune-related surface markers on IFN-γ treated hChonJ. After hChonJ cells were exposed to 300 IU/mL of IFN-γ, the cells were labeled with antibodies against immune-related surface markers, including MHC class I (HLA-ABC), MHC class II (HLA-DR), co-stimulatory molecules (CD80 and CD86), co-inhibitory molecules (PD-L1 and PD-L2) and immunoglobulin G1 isotype control. The expression of MHC class I and II molecules ( a ) was increased, but there was no change in the expression level of the co-stimulatory molecules CD80 and CD86 ( b ). In the case of co-inhibitory molecules, PD-L1 expression was increased and the expression of PD-L2 was detected which was not expressed on IFN-γ untreated hChonJ cells ( c ). These results are the representative of at least 3 independent experiments

    Article Snippet: Recombinant interferon (IFN)-γ was purchased from Peprotech (NJ, USA), isopropyl alcohol, mitomycin C and phytohemagglutinin were purchased from Sigma Chemical (MO, USA) and 3 H-thymidine was purchased from American Radiolabeled Chemicals (MO, USA).

    Techniques: Flow Cytometry, Cytometry, Labeling, Expressing

    Directed transport of GAP43 by transient piggybacking on exocytic vesicles. (A) Flux analysis of photoactivated protein in processes of transfected PC12 cells. Top, schematic showing the region of photoactivation (gray box) and the position of the recording regions distal and proximal from the center of activation. Bottom, ratios of distal to proximal fluorescence at different times after activation. Mean ± SEM, n = 7–9. The percentage of processes that show higher distal than proximal fluorescence is given in the boxes. *Significantly higher values compared with 1.0. (B) TIRF image of a part of a cell body of a living PC12 cell expressing GAP43-HaloTag (labeled with HTL-OG; green) and synaptophysin-mCherry (red). The focal plane was adjusted in such a way as to visualize a region above the cellular contact site, and the nucleus is visible at the right. Note the presence of green and red structures with vesicular appearance. Note that some structures show yellow fluorescence (arrowheads) indicative of colocalization of GAP43 with vesicular structures. Scale bar, 10 μm. Bottom, time series of TIRF micrographs at the transition of a neurite shaft (n.s.) to the growth cone (g.c.). An example of a particle showing colocalization of GAP43 and synaptophysin, which can be tracked for several seconds, is shown. Scale bar, 10 μm (left), 5 μm (right). The table gives numbers for individual tracking events with colocalization of HTL-OG–labeled GAP43-HaloTag and synaptophysin-mCherry. Note that double-labeled particles move with an average speed of 1.5 ± 0.2 μm/s ( n = 10), similar to the speed of fast axonal transport. Statistical analysis was performed using Student's t test. *, p

    Journal: Molecular Biology of the Cell

    Article Title: Interplay between phosphorylation and palmitoylation mediates plasma membrane targeting and sorting of GAP43

    doi: 10.1091/mbc.E13-12-0737

    Figure Lengend Snippet: Directed transport of GAP43 by transient piggybacking on exocytic vesicles. (A) Flux analysis of photoactivated protein in processes of transfected PC12 cells. Top, schematic showing the region of photoactivation (gray box) and the position of the recording regions distal and proximal from the center of activation. Bottom, ratios of distal to proximal fluorescence at different times after activation. Mean ± SEM, n = 7–9. The percentage of processes that show higher distal than proximal fluorescence is given in the boxes. *Significantly higher values compared with 1.0. (B) TIRF image of a part of a cell body of a living PC12 cell expressing GAP43-HaloTag (labeled with HTL-OG; green) and synaptophysin-mCherry (red). The focal plane was adjusted in such a way as to visualize a region above the cellular contact site, and the nucleus is visible at the right. Note the presence of green and red structures with vesicular appearance. Note that some structures show yellow fluorescence (arrowheads) indicative of colocalization of GAP43 with vesicular structures. Scale bar, 10 μm. Bottom, time series of TIRF micrographs at the transition of a neurite shaft (n.s.) to the growth cone (g.c.). An example of a particle showing colocalization of GAP43 and synaptophysin, which can be tracked for several seconds, is shown. Scale bar, 10 μm (left), 5 μm (right). The table gives numbers for individual tracking events with colocalization of HTL-OG–labeled GAP43-HaloTag and synaptophysin-mCherry. Note that double-labeled particles move with an average speed of 1.5 ± 0.2 μm/s ( n = 10), similar to the speed of fast axonal transport. Statistical analysis was performed using Student's t test. *, p

    Article Snippet: Mouse synaptophysin-mCherry was synthesized by custom gene synthesis (Life Technologies, Darmstadt, Germany) containing restriction sites for Bam HI and Xba I and cloned into pCDNA3.1(+) (Invitrogen).

    Techniques: Transfection, Activation Assay, Fluorescence, Expressing, Labeling