2-mercaptoethanol Search Results


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  • 99
    Thermo Fisher 2 mercaptoethanol 2 me
    2 Mercaptoethanol 2 Me, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 mercaptoethanol 2 me/product/Thermo Fisher
    Average 99 stars, based on 113 article reviews
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    99
    Millipore 2 mercaptoethanol 2 me
    2 Mercaptoethanol 2 Me, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 mercaptoethanol 2 me/product/Millipore
    Average 99 stars, based on 148 article reviews
    Price from $9.99 to $1999.99
    2 mercaptoethanol 2 me - by Bioz Stars, 2020-05
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    99
    Bio-Rad 2mercaptoethanol
    Cyclic voltammograms of the step-wise surface fabrication of ( A ) YNGRT-Au and ( B ) VLGXE-Au: (a) bare Au; (b) Lip-NHS; (c) peptide; (d) ethanolamine; and (e) <t>2-mercaptoethanol.</t>
    2mercaptoethanol, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2mercaptoethanol/product/Bio-Rad
    Average 99 stars, based on 194 article reviews
    Price from $9.99 to $1999.99
    2mercaptoethanol - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher 2mercaptoethanol
    EPO-independent activation of BCL-XL and BCL2 expression at discrete stages of (pro)erythroblast development. (A) In primary bone marrow erythroblasts, neither BCL-XL nor BCL2 expression is EPO-modulated. Kit pos CD71 high Ter119 neg erythroblasts were expanded from wt-EPOR bone marrow preparations. Cells then were cultured for 5 hours in 50 μg/mL transferrin, 0.1% BSA, 15 ng/mL insulin, 0.1 mM <t>2-ME,</t> IMDM. EPO was then added (2.5 U/mL), and at 0, 2.5, and 7.5 hours cell lysates were prepared. Lysates then were analyzed by Western blotting for levels of BCL-XL, BCL2, PIM1, and beta-tubulin. In parallel, possible EPO modulation of Bcl-x or Bcl2 transcripts also was analyzed, here at 0, 30, 90, and 270 minutes of EPO exposure. Values are mean-fold modulation plus or minus SE. (B) Mapping of EPOR subdomains to EPO-regulated survival factor circuits. Presently defined EPO-modulated transcriptional response circuits are outlined. An EPOR JAK2-only circuit mediates Foxo3a, Bim , and Trb2 repression. In parallel, an EPOR/PY343/STAT5 axis enhances Pim1 and Pim3 expression, and affords EPO induction of Irs2, S3G , and Trb3 .
    2mercaptoethanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2mercaptoethanol/product/Thermo Fisher
    Average 99 stars, based on 15 article reviews
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    2mercaptoethanol - by Bioz Stars, 2020-05
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    89
    Kodak Corp 2 mercaptoethanol 2 me
    EPO-independent activation of BCL-XL and BCL2 expression at discrete stages of (pro)erythroblast development. (A) In primary bone marrow erythroblasts, neither BCL-XL nor BCL2 expression is EPO-modulated. Kit pos CD71 high Ter119 neg erythroblasts were expanded from wt-EPOR bone marrow preparations. Cells then were cultured for 5 hours in 50 μg/mL transferrin, 0.1% BSA, 15 ng/mL insulin, 0.1 mM <t>2-ME,</t> IMDM. EPO was then added (2.5 U/mL), and at 0, 2.5, and 7.5 hours cell lysates were prepared. Lysates then were analyzed by Western blotting for levels of BCL-XL, BCL2, PIM1, and beta-tubulin. In parallel, possible EPO modulation of Bcl-x or Bcl2 transcripts also was analyzed, here at 0, 30, 90, and 270 minutes of EPO exposure. Values are mean-fold modulation plus or minus SE. (B) Mapping of EPOR subdomains to EPO-regulated survival factor circuits. Presently defined EPO-modulated transcriptional response circuits are outlined. An EPOR JAK2-only circuit mediates Foxo3a, Bim , and Trb2 repression. In parallel, an EPOR/PY343/STAT5 axis enhances Pim1 and Pim3 expression, and affords EPO induction of Irs2, S3G , and Trb3 .
    2 Mercaptoethanol 2 Me, supplied by Kodak Corp, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 mercaptoethanol 2 me/product/Kodak Corp
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    89
    FUJIFILM 2 mercaptoethanol 2 me
    EPO-independent activation of BCL-XL and BCL2 expression at discrete stages of (pro)erythroblast development. (A) In primary bone marrow erythroblasts, neither BCL-XL nor BCL2 expression is EPO-modulated. Kit pos CD71 high Ter119 neg erythroblasts were expanded from wt-EPOR bone marrow preparations. Cells then were cultured for 5 hours in 50 μg/mL transferrin, 0.1% BSA, 15 ng/mL insulin, 0.1 mM <t>2-ME,</t> IMDM. EPO was then added (2.5 U/mL), and at 0, 2.5, and 7.5 hours cell lysates were prepared. Lysates then were analyzed by Western blotting for levels of BCL-XL, BCL2, PIM1, and beta-tubulin. In parallel, possible EPO modulation of Bcl-x or Bcl2 transcripts also was analyzed, here at 0, 30, 90, and 270 minutes of EPO exposure. Values are mean-fold modulation plus or minus SE. (B) Mapping of EPOR subdomains to EPO-regulated survival factor circuits. Presently defined EPO-modulated transcriptional response circuits are outlined. An EPOR JAK2-only circuit mediates Foxo3a, Bim , and Trb2 repression. In parallel, an EPOR/PY343/STAT5 axis enhances Pim1 and Pim3 expression, and affords EPO induction of Irs2, S3G , and Trb3 .
    2 Mercaptoethanol 2 Me, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 mercaptoethanol 2 me/product/FUJIFILM
    Average 89 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Cyclic voltammograms of the step-wise surface fabrication of ( A ) YNGRT-Au and ( B ) VLGXE-Au: (a) bare Au; (b) Lip-NHS; (c) peptide; (d) ethanolamine; and (e) 2-mercaptoethanol.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Electrochemical Characterization of Protein Adsorption onto YNGRT-Au and VLGXE-Au Surfaces

    doi: 10.3390/s150819429

    Figure Lengend Snippet: Cyclic voltammograms of the step-wise surface fabrication of ( A ) YNGRT-Au and ( B ) VLGXE-Au: (a) bare Au; (b) Lip-NHS; (c) peptide; (d) ethanolamine; and (e) 2-mercaptoethanol.

    Article Snippet: 2-mercaptoethanol was purchased from BioRad (Hercules, CA, USA).

    Techniques:

    Nyquist plots of the step-wise surface fabrication of ( A ) YNGRT-Au and ( B ) VLGXE-Au: (a) bare Au, (b) Lip-NHS, (c) peptide, (d) ethanolamine and (e) 2-mercaptoethanol (inset: equivalent circuit used for fitting impedance data).

    Journal: Sensors (Basel, Switzerland)

    Article Title: Electrochemical Characterization of Protein Adsorption onto YNGRT-Au and VLGXE-Au Surfaces

    doi: 10.3390/s150819429

    Figure Lengend Snippet: Nyquist plots of the step-wise surface fabrication of ( A ) YNGRT-Au and ( B ) VLGXE-Au: (a) bare Au, (b) Lip-NHS, (c) peptide, (d) ethanolamine and (e) 2-mercaptoethanol (inset: equivalent circuit used for fitting impedance data).

    Article Snippet: 2-mercaptoethanol was purchased from BioRad (Hercules, CA, USA).

    Techniques:

    Stepwise procedure for covalent immobilization of peptide (YNGRT or VLGXE) on Au: ( a ) Lipoic acid N-hydroxysuccinimide ester (Lip-NHS); ( b ) peptide in 10% acetonitrile (YNGRT or VLGXE); ( c ) ethanolamine; ( d ) 2-mercaptoethanol.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Electrochemical Characterization of Protein Adsorption onto YNGRT-Au and VLGXE-Au Surfaces

    doi: 10.3390/s150819429

    Figure Lengend Snippet: Stepwise procedure for covalent immobilization of peptide (YNGRT or VLGXE) on Au: ( a ) Lipoic acid N-hydroxysuccinimide ester (Lip-NHS); ( b ) peptide in 10% acetonitrile (YNGRT or VLGXE); ( c ) ethanolamine; ( d ) 2-mercaptoethanol.

    Article Snippet: 2-mercaptoethanol was purchased from BioRad (Hercules, CA, USA).

    Techniques:

    Plot of change in R ct as a function of protein solution during ( A ) CD13; ( B ) BSA; and ( C ) mucin protein adsorption on VLGXE-Au with various surfaces: (a) ethanolamine/2-mercaptoethanol, (b) ethanolamine/hexanethiol, (c) n-butylamine/hexanethiol, (d) n-butylamine/2-mercaptoethanol (protein concentration was 10 µg·mL −1 ).

    Journal: Sensors (Basel, Switzerland)

    Article Title: Electrochemical Characterization of Protein Adsorption onto YNGRT-Au and VLGXE-Au Surfaces

    doi: 10.3390/s150819429

    Figure Lengend Snippet: Plot of change in R ct as a function of protein solution during ( A ) CD13; ( B ) BSA; and ( C ) mucin protein adsorption on VLGXE-Au with various surfaces: (a) ethanolamine/2-mercaptoethanol, (b) ethanolamine/hexanethiol, (c) n-butylamine/hexanethiol, (d) n-butylamine/2-mercaptoethanol (protein concentration was 10 µg·mL −1 ).

    Article Snippet: 2-mercaptoethanol was purchased from BioRad (Hercules, CA, USA).

    Techniques: Adsorption, Protein Concentration

    Plot of change in R ct as a function of protein solution during ( A ) CD13; ( B ) BSA; and ( C ) mucin protein adsorption on YNGRT-Au with various surfaces: (a) ethanolamine/2-mercaptoethanol, (b) ethanolamine/hexanethiol, (c) n -butylamine/hexanethiol, (d) n -butylamine/2-mercaptoethanol (protein concentration was 10 µg·mL −1 ).

    Journal: Sensors (Basel, Switzerland)

    Article Title: Electrochemical Characterization of Protein Adsorption onto YNGRT-Au and VLGXE-Au Surfaces

    doi: 10.3390/s150819429

    Figure Lengend Snippet: Plot of change in R ct as a function of protein solution during ( A ) CD13; ( B ) BSA; and ( C ) mucin protein adsorption on YNGRT-Au with various surfaces: (a) ethanolamine/2-mercaptoethanol, (b) ethanolamine/hexanethiol, (c) n -butylamine/hexanethiol, (d) n -butylamine/2-mercaptoethanol (protein concentration was 10 µg·mL −1 ).

    Article Snippet: 2-mercaptoethanol was purchased from BioRad (Hercules, CA, USA).

    Techniques: Adsorption, Protein Concentration

    EPO-independent activation of BCL-XL and BCL2 expression at discrete stages of (pro)erythroblast development. (A) In primary bone marrow erythroblasts, neither BCL-XL nor BCL2 expression is EPO-modulated. Kit pos CD71 high Ter119 neg erythroblasts were expanded from wt-EPOR bone marrow preparations. Cells then were cultured for 5 hours in 50 μg/mL transferrin, 0.1% BSA, 15 ng/mL insulin, 0.1 mM 2-ME, IMDM. EPO was then added (2.5 U/mL), and at 0, 2.5, and 7.5 hours cell lysates were prepared. Lysates then were analyzed by Western blotting for levels of BCL-XL, BCL2, PIM1, and beta-tubulin. In parallel, possible EPO modulation of Bcl-x or Bcl2 transcripts also was analyzed, here at 0, 30, 90, and 270 minutes of EPO exposure. Values are mean-fold modulation plus or minus SE. (B) Mapping of EPOR subdomains to EPO-regulated survival factor circuits. Presently defined EPO-modulated transcriptional response circuits are outlined. An EPOR JAK2-only circuit mediates Foxo3a, Bim , and Trb2 repression. In parallel, an EPOR/PY343/STAT5 axis enhances Pim1 and Pim3 expression, and affords EPO induction of Irs2, S3G , and Trb3 .

    Journal: Blood

    Article Title: EPO receptor circuits for primary erythroblast survival

    doi: 10.1182/blood-2007-10-119743

    Figure Lengend Snippet: EPO-independent activation of BCL-XL and BCL2 expression at discrete stages of (pro)erythroblast development. (A) In primary bone marrow erythroblasts, neither BCL-XL nor BCL2 expression is EPO-modulated. Kit pos CD71 high Ter119 neg erythroblasts were expanded from wt-EPOR bone marrow preparations. Cells then were cultured for 5 hours in 50 μg/mL transferrin, 0.1% BSA, 15 ng/mL insulin, 0.1 mM 2-ME, IMDM. EPO was then added (2.5 U/mL), and at 0, 2.5, and 7.5 hours cell lysates were prepared. Lysates then were analyzed by Western blotting for levels of BCL-XL, BCL2, PIM1, and beta-tubulin. In parallel, possible EPO modulation of Bcl-x or Bcl2 transcripts also was analyzed, here at 0, 30, 90, and 270 minutes of EPO exposure. Values are mean-fold modulation plus or minus SE. (B) Mapping of EPOR subdomains to EPO-regulated survival factor circuits. Presently defined EPO-modulated transcriptional response circuits are outlined. An EPOR JAK2-only circuit mediates Foxo3a, Bim , and Trb2 repression. In parallel, an EPOR/PY343/STAT5 axis enhances Pim1 and Pim3 expression, and affords EPO induction of Irs2, S3G , and Trb3 .

    Article Snippet: Red cells then were lysed, and progenitors were collected through 50% FBS in PBS., For ex vivo expansions, cells were plated at 8 × 105 cells/mL (7 mL per 100-mm dish) in StemPro-34 (Invitrogen) supplemented with 2.5 U/mL EPO, 100 ng/mL mSCF, 1 μM dexamethasone, 1 μM beta-estradiol, 75 μg/mL h-transferrin, (Sigma-Aldrich, St Louis, MO), 0.5% BSA (StemCell Technologies, Vancouver, BC), 0.1 mM 2-mercaptoethanol, 100 U/mL penicillin G, 100 μg/mL streptomycin, 0.25 μg/mL amphotericin B (1XPSF) (Invitrogen), and 1.5 mM l -glutamine (ie, “SP34-EX” medium).

    Techniques: Activation Assay, Expressing, Cell Culture, Western Blot

    EPO modulation of endogenous S3G expression in wt-EPOR, EPOR-H, and EPOR-HM erythroblasts, and S3G effects on erythroid progenitor cell survival. (A) Kit pos CD71 high Ter119 neg erythroblasts were isolated from wt-EPOR, EPOR-H, and EPOR-HM bone marrow expansion cultures. Cells then were cultured for 5 hours in 50 μg/mL transferrin, 0.1% BSA, 0.1 mM 2-ME, 15 ng/mL insulin, IMDM. At the time intervals indicated, cell lysates were prepared and analyzed for S3G expression via Western blotting. Outcomes for the wt-EPOR are illustrated in panel Ai, and for EPOR-HM and EPO-H alleles in panel Aii. (B) Epo-dependent G1E/JC4 cells were transduced with a MIGR-S3G retroviral construct, or with an empty MIGR vector as a negative control. For G1E/JC4-S3G and G1E/JC4-MIGR cells in exponential growth phase, EPO was withdrawn for 6 hours and subsequently was provided at the doses indicated. At 24 hours, frequencies of apoptotic cells were assayed by staining with annexin-V and flow cytometry. Transduction sets nos. 1 and 2 represent independently transduced cell populations. (C) Average effects of S3G on G1E/JC4 cell survival for 4 independent analyses also are illustrated. Values are normalized means plus or minus SE. (D) Via Western blotting of cellular fractions and concentrated media, ectopically expressed S3G was observed to localize to a cytosolic fraction. S3G's basic structure, including its serpin domain and reactive center loop (RCL), also is diagrammed.

    Journal: Blood

    Article Title: EPO receptor circuits for primary erythroblast survival

    doi: 10.1182/blood-2007-10-119743

    Figure Lengend Snippet: EPO modulation of endogenous S3G expression in wt-EPOR, EPOR-H, and EPOR-HM erythroblasts, and S3G effects on erythroid progenitor cell survival. (A) Kit pos CD71 high Ter119 neg erythroblasts were isolated from wt-EPOR, EPOR-H, and EPOR-HM bone marrow expansion cultures. Cells then were cultured for 5 hours in 50 μg/mL transferrin, 0.1% BSA, 0.1 mM 2-ME, 15 ng/mL insulin, IMDM. At the time intervals indicated, cell lysates were prepared and analyzed for S3G expression via Western blotting. Outcomes for the wt-EPOR are illustrated in panel Ai, and for EPOR-HM and EPO-H alleles in panel Aii. (B) Epo-dependent G1E/JC4 cells were transduced with a MIGR-S3G retroviral construct, or with an empty MIGR vector as a negative control. For G1E/JC4-S3G and G1E/JC4-MIGR cells in exponential growth phase, EPO was withdrawn for 6 hours and subsequently was provided at the doses indicated. At 24 hours, frequencies of apoptotic cells were assayed by staining with annexin-V and flow cytometry. Transduction sets nos. 1 and 2 represent independently transduced cell populations. (C) Average effects of S3G on G1E/JC4 cell survival for 4 independent analyses also are illustrated. Values are normalized means plus or minus SE. (D) Via Western blotting of cellular fractions and concentrated media, ectopically expressed S3G was observed to localize to a cytosolic fraction. S3G's basic structure, including its serpin domain and reactive center loop (RCL), also is diagrammed.

    Article Snippet: Red cells then were lysed, and progenitors were collected through 50% FBS in PBS., For ex vivo expansions, cells were plated at 8 × 105 cells/mL (7 mL per 100-mm dish) in StemPro-34 (Invitrogen) supplemented with 2.5 U/mL EPO, 100 ng/mL mSCF, 1 μM dexamethasone, 1 μM beta-estradiol, 75 μg/mL h-transferrin, (Sigma-Aldrich, St Louis, MO), 0.5% BSA (StemCell Technologies, Vancouver, BC), 0.1 mM 2-mercaptoethanol, 100 U/mL penicillin G, 100 μg/mL streptomycin, 0.25 μg/mL amphotericin B (1XPSF) (Invitrogen), and 1.5 mM l -glutamine (ie, “SP34-EX” medium).

    Techniques: Expressing, Isolation, Cell Culture, Western Blot, Transduction, Construct, Plasmid Preparation, Negative Control, Staining, Flow Cytometry, Cytometry