Journal: Toxicology Reports
Article Title: Comparison of the neurotoxicities between volatile organic compounds and fragrant organic compounds on human neuroblastoma SK-N-SH cells and primary cultured rat neurons
Figure Lengend Snippet: Inhibitory dose–response curves for VOCs and FOCs in the human neuroblastoma cell line SK-N-SH. Various concentrations of VOCs (A: toluene, B: n-octanal, C: nonanal, D: 2-ethyl-1-hexanol) and FOCs (E: linalool, F: cis -3-hexen-1-ol, G: n-phenethyl alcohol) were applied, and death of SK-N-SH cells was determined by the MTT assay. Each value is the mean + S.E. of cell viability obtained for three experiments. Theoretical curves were drawn according to the equation V = V max /(1 + IC 50 / A ) n . V , A , and n represent cell viability, concentrations of VOCs and FOCs, and Hill coefficients, respectively.
Article Snippet: 2.1 Chemicals Toluene, linalool, cis -3-hexen-1-ol, isoamyl alcohol, n-propyl alcohol, and phenethyl alcohol were obtained from Wako Pure Chemical Industries (Japan). n-Octanal, nonanal, and 2-ethyl-1-hexanol were obtained from Tokyo Chemical Industry Co., Ltd. (Japan).
Techniques: MTT Assay