2-aminoethoxydiphenyl borate Search Results


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  • 93
    Millipore ip3 receptor inhibitor 2 apb
    Effect of retinoschisin on Ca 2+ signaling. (A) Rs1h -/Y retinal explants were treated for 10 and 30 min with retinoschisin, RS1-C59S, or control protein. After treatment, the cells were subjected to Western blot analyses with antibodies against phosphorylated Camk2 (pCamk2), total Camk2, and Actb as control. Densitometric quantification was performed with immunoblots from five independent experiments. Signals for pCamk2 and Camk2 were normalized against Actb and calibrated against the control. Data represent mean ± SD. (B) Rs1h -/Y retinal explants were treated for 30 min with Ca 2+ signaling (IP3 receptor) inhibitor <t>2-APB.</t> To assess an effect of the Ca 2+ pathway on Erk1/2 activation, the cells were subjected to Western blot analyses with antibodies against phosphorylated Erk1 and Erk2 (pErk1/2), total Erk1 and Erk2 (Erk1/2), and Actb as control. Underlined asterisk marks statistically significant ( p
    Ip3 Receptor Inhibitor 2 Apb, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Tocris 2 apb
    Effect of increasing temperature on response to <t>2-APB</t> in vagal sensory neurons isolated from WT and TRPV1−/− mice. A : experimental record illustrating that the 2-APB (0.3 mM, 2 s)-evoked current was augmented in a WT neuron (16.8 pF) when
    2 Apb, supplied by Tocris, used in various techniques. Bioz Stars score: 97/100, based on 342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore aminoethoxydiphenyl borate 2 apb
    Skeletal muscle L-type current is blocked by nonspecific cation channel antagonists. Acute block of L-type currents evoked by step depolarizations to +30 mV by 100 μM Gd 3+ (A), 100 μM La 3+ (B), 100 μM <t>2-APB</t> (C), and 20 μM SKF 96356 (D). The test pulse was applied after a prepulse protocol (see Materials and methods) ( Adams et al., 1990 ). Results of these experiments and application of DMSO vehicle (0.01%) control experiments are summarized in E. For trivalent cations, maximal block was generally achieved in
    Aminoethoxydiphenyl Borate 2 Apb, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Merck & Co 2 aminoethoxydiphenyl borate 2 apb
    Effects of store-operated calcium channel blockers on cell survival or injuries of normal and hepatic ischemia-reperfusion injuried hepatocytes. A: MTT assay of normal hepatocytes alone or co-cultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB, or 100 μmol/L La 3+ ( n = 4), the cell survival rate is not significantly different ( P > 0.05); B: MTT assay of HIRI hepatocytes alone or cocultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB or 100 μmol/L La 3+ ( n = 4), the average optical absorbance value in hepatocytes pretreated with 100 μmol/L 2-APB (0.79 ± 0.05) was higher than in untreated hepatocytes (0.69 ± 0.04), a P = 0.027; C: Compared with normal hepatocytes, HIRI hepatocytes incurred cell damage ( n = 4), a P = 0.029. HIRI: Hepatic ischemia-reperfusion injury; 2-APB: <t>2-aminoethoxydiphenyl</t> borate; DMSO: Dimethylsulfoxide; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
    2 Aminoethoxydiphenyl Borate 2 Apb, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher ireland 2 aminoethoxydiphenyl borate
    Effects of store-operated calcium channel blockers on cell survival or injuries of normal and hepatic ischemia-reperfusion injuried hepatocytes. A: MTT assay of normal hepatocytes alone or co-cultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB, or 100 μmol/L La 3+ ( n = 4), the cell survival rate is not significantly different ( P > 0.05); B: MTT assay of HIRI hepatocytes alone or cocultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB or 100 μmol/L La 3+ ( n = 4), the average optical absorbance value in hepatocytes pretreated with 100 μmol/L 2-APB (0.79 ± 0.05) was higher than in untreated hepatocytes (0.69 ± 0.04), a P = 0.027; C: Compared with normal hepatocytes, HIRI hepatocytes incurred cell damage ( n = 4), a P = 0.029. HIRI: Hepatic ischemia-reperfusion injury; 2-APB: <t>2-aminoethoxydiphenyl</t> borate; DMSO: Dimethylsulfoxide; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
    Ireland 2 Aminoethoxydiphenyl Borate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cayman Chemical 2 apb
    IP 3 -mediated calcium signaling participates in fractalkine-induced [Ca 2+ ] i release in BV-2 cells. ( A ) [Ca 2+ ] i was increased by fractalkine in the media with calcium. ( B ) [Ca 2+ ] i was increased by fractalkine in the media with calcium-free. ( C ) The elevation of [Ca 2+ ] i by fractalkine was inhibited by <t>2-APB.</t> * P
    2 Apb, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 92/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore aceto methoxy 2 aminoethoxydiphenyl borate
    IP 3 -mediated calcium signaling participates in fractalkine-induced [Ca 2+ ] i release in BV-2 cells. ( A ) [Ca 2+ ] i was increased by fractalkine in the media with calcium. ( B ) [Ca 2+ ] i was increased by fractalkine in the media with calcium-free. ( C ) The elevation of [Ca 2+ ] i by fractalkine was inhibited by <t>2-APB.</t> * P
    Aceto Methoxy 2 Aminoethoxydiphenyl Borate, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific 2 aminoethoxydiphenyl borate
    Involvement of intracellular calcium stores in the mechanism of action of PACAP38 in cerebellar granule cells . (A–D) Effect of a single application of 10 −6 M PACAP38 on the amplitude (A,C) and the area under the curve (B,D) of the [Ca 2+ ] i response in the absence (black bars) or presence (white bars) of 10 −6 M thapsigargin, a Ca 2+ ATPase inhibitor, or 10 −5 M 2APB, a membrane permeable d -myo-inositol 1,4,5-trisphosphate receptor antagonist. Thapsigargin and 2APB were added 15 and 30 min before application of the pulse of PACAP, respectively. (A,C) Histograms showing the distribution of granule cells according to the amplitude of their response to PACAP. Each graph represents the analysis of a least 50 cells from 3 different cultures. 2APB, <t>2-aminoethoxydiphenyl</t> borate; AUC, area under the curve. *** p
    2 Aminoethoxydiphenyl Borate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Tokyo Chemical Industry 2 aminoethoxydiphenyl borate
    Involvement of intracellular calcium stores in the mechanism of action of PACAP38 in cerebellar granule cells . (A–D) Effect of a single application of 10 −6 M PACAP38 on the amplitude (A,C) and the area under the curve (B,D) of the [Ca 2+ ] i response in the absence (black bars) or presence (white bars) of 10 −6 M thapsigargin, a Ca 2+ ATPase inhibitor, or 10 −5 M 2APB, a membrane permeable d -myo-inositol 1,4,5-trisphosphate receptor antagonist. Thapsigargin and 2APB were added 15 and 30 min before application of the pulse of PACAP, respectively. (A,C) Histograms showing the distribution of granule cells according to the amplitude of their response to PACAP. Each graph represents the analysis of a least 50 cells from 3 different cultures. 2APB, <t>2-aminoethoxydiphenyl</t> borate; AUC, area under the curve. *** p
    2 Aminoethoxydiphenyl Borate, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Enzo Biochem 2 aminoethoxydiphenyl borate
    Ca 2+ wave frequency with and without ET-1 (1 nM) in arteries from IH rats in the absence and presence of the nonspecific IP 3 R inhibitor <t>2-aminoethoxydiphenyl</t> borate (2-APB; 50 μM) and the phospholipase C inhibitor U-73122 (1 μM). Values
    2 Aminoethoxydiphenyl Borate, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Selleck Chemicals 2 aminoethoxydiphenyl borate
    Ca 2+ wave frequency with and without ET-1 (1 nM) in arteries from IH rats in the absence and presence of the nonspecific IP 3 R inhibitor <t>2-aminoethoxydiphenyl</t> borate (2-APB; 50 μM) and the phospholipase C inhibitor U-73122 (1 μM). Values
    2 Aminoethoxydiphenyl Borate, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology 2 aminoethoxydiphenyl borate
    Ca 2+ wave frequency with and without ET-1 (1 nM) in arteries from IH rats in the absence and presence of the nonspecific IP 3 R inhibitor <t>2-aminoethoxydiphenyl</t> borate (2-APB; 50 μM) and the phospholipase C inhibitor U-73122 (1 μM). Values
    2 Aminoethoxydiphenyl Borate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher 2 apb
    Knockdown of STIM1 attenuates SOCE in human NPCs. (A,B) Ca 2+ -responses during ER-store release and SOCE induced by Thapsigargin (TG, 10 μM) measured using the ratiometric Ca 2+ -indicator indo-1-AM in wild-type (WT) hNPCs (A) or hNPCs treated with pharmacological inhibitors of SOCE, BTP-2 and <t>2-APB</t> at the indicated concentrations or DMSO as a solvent control (B) . Each trace represents the mean ±SEM for 25–100 cells. Ionomycin (Iono, 10 μM) was added at the end of each imaging to determine the peak F405/485 ratio obtained after saturation of the Ca 2+ -indicator with Ca 2+ . (C) Box plots quantifying the peak F405/485 values for store-release and SOCE in the indicated treatment conditions. Mann–Whitney U test with Bonferroni correction. p = 1.819 × 10 -23 for DMSO control compared to BTP-2 treatment and p = 1.442 × 10 -45 for DMSO control compared to 2-APB treatment. (D) (Top) A representative Western blot showing levels of STIM1 protein in hNPCs transduced with an NTC (non-targeting control) or an sh-RNA targeting STIM1 ( STIM1 KD). Actin serves as the loading control. (Bottom) Quantification of STIM1 band intensities normalized to the loading control Actin from three independent biological replicates ( p = 0.00069, Student’s t -test). (E) Ca 2+ -responses during store-release and SOCE in hNPCs transduced with NTC and STIM1 KD. (F) Box plots quantifying the peak F405/485 values for store-release and SOCE in the indicated genotypes. Peak F405/485 for store-release were not significantly different between NTC and STIM1 KD NPCs. p = 0.0001 for peak F405/485 during SOCE compared between NTC- and STIM1 KD NPCs. (G) Quantification of basal cytosolic [Ca 2+ ] values using Fura-2-AM in NTC- and STIM1 KD NPCs ( p = 1.115 × 10 -8 . Mann–Whitney U test. ( ∗∗∗ Indicates p
    2 Apb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of retinoschisin on Ca 2+ signaling. (A) Rs1h -/Y retinal explants were treated for 10 and 30 min with retinoschisin, RS1-C59S, or control protein. After treatment, the cells were subjected to Western blot analyses with antibodies against phosphorylated Camk2 (pCamk2), total Camk2, and Actb as control. Densitometric quantification was performed with immunoblots from five independent experiments. Signals for pCamk2 and Camk2 were normalized against Actb and calibrated against the control. Data represent mean ± SD. (B) Rs1h -/Y retinal explants were treated for 30 min with Ca 2+ signaling (IP3 receptor) inhibitor 2-APB. To assess an effect of the Ca 2+ pathway on Erk1/2 activation, the cells were subjected to Western blot analyses with antibodies against phosphorylated Erk1 and Erk2 (pErk1/2), total Erk1 and Erk2 (Erk1/2), and Actb as control. Underlined asterisk marks statistically significant ( p

    Journal: Molecular Biology of the Cell

    Article Title: Retinoschisin is linked to retinal Na/K-ATPase signaling and localization

    doi: 10.1091/mbc.E17-01-0064

    Figure Lengend Snippet: Effect of retinoschisin on Ca 2+ signaling. (A) Rs1h -/Y retinal explants were treated for 10 and 30 min with retinoschisin, RS1-C59S, or control protein. After treatment, the cells were subjected to Western blot analyses with antibodies against phosphorylated Camk2 (pCamk2), total Camk2, and Actb as control. Densitometric quantification was performed with immunoblots from five independent experiments. Signals for pCamk2 and Camk2 were normalized against Actb and calibrated against the control. Data represent mean ± SD. (B) Rs1h -/Y retinal explants were treated for 30 min with Ca 2+ signaling (IP3 receptor) inhibitor 2-APB. To assess an effect of the Ca 2+ pathway on Erk1/2 activation, the cells were subjected to Western blot analyses with antibodies against phosphorylated Erk1 and Erk2 (pErk1/2), total Erk1 and Erk2 (Erk1/2), and Actb as control. Underlined asterisk marks statistically significant ( p

    Article Snippet: Src kinase inhibitor PP2 and IP3 receptor inhibitor 2-APB were obtained from Sigma- Aldrich and added at a final concentration of 10 or 250 µM, respectively.

    Techniques: Western Blot, Activation Assay

    Effect of increasing temperature on response to 2-APB in vagal sensory neurons isolated from WT and TRPV1−/− mice. A : experimental record illustrating that the 2-APB (0.3 mM, 2 s)-evoked current was augmented in a WT neuron (16.8 pF) when

    Journal:

    Article Title: Lack of potentiating effect of increasing temperature on responses to chemical activators in vagal sensory neurons isolated from TRPV1-null mice

    doi: 10.1152/ajplung.90385.2008

    Figure Lengend Snippet: Effect of increasing temperature on response to 2-APB in vagal sensory neurons isolated from WT and TRPV1−/− mice. A : experimental record illustrating that the 2-APB (0.3 mM, 2 s)-evoked current was augmented in a WT neuron (16.8 pF) when

    Article Snippet: Furthermore, increasing temperature generated a pronounced potentiating effect on the responses of vagal sensory neuron to 2-APB and acid in WT mice, similar to that previously observed in neurons isolated from rats ( ); in sharp contrast, the same increase in temperature had no effect on the responses of these neurons to the same stimuli in TRPV1−/− mice, indicating that the sensitizing effect of hyperthermia on these sensory neurons is almost exclusively mediated through the TRPV1 channel.

    Techniques: Isolation, Mouse Assay

    Responses to Cap and 2-aminoethoxydiphenyl borate (2-APB) in vagal sensory neurons isolated from WT and TRPV1−/− mice. A : experimental record illustrating Cap (1 μM, 4 s)-evoked inward current in a WT (25.1 pF) neuron but not in

    Journal:

    Article Title: Lack of potentiating effect of increasing temperature on responses to chemical activators in vagal sensory neurons isolated from TRPV1-null mice

    doi: 10.1152/ajplung.90385.2008

    Figure Lengend Snippet: Responses to Cap and 2-aminoethoxydiphenyl borate (2-APB) in vagal sensory neurons isolated from WT and TRPV1−/− mice. A : experimental record illustrating Cap (1 μM, 4 s)-evoked inward current in a WT (25.1 pF) neuron but not in

    Article Snippet: Furthermore, increasing temperature generated a pronounced potentiating effect on the responses of vagal sensory neuron to 2-APB and acid in WT mice, similar to that previously observed in neurons isolated from rats ( ); in sharp contrast, the same increase in temperature had no effect on the responses of these neurons to the same stimuli in TRPV1−/− mice, indicating that the sensitizing effect of hyperthermia on these sensory neurons is almost exclusively mediated through the TRPV1 channel.

    Techniques: Isolation, Mouse Assay

    Skeletal muscle L-type current is blocked by nonspecific cation channel antagonists. Acute block of L-type currents evoked by step depolarizations to +30 mV by 100 μM Gd 3+ (A), 100 μM La 3+ (B), 100 μM 2-APB (C), and 20 μM SKF 96356 (D). The test pulse was applied after a prepulse protocol (see Materials and methods) ( Adams et al., 1990 ). Results of these experiments and application of DMSO vehicle (0.01%) control experiments are summarized in E. For trivalent cations, maximal block was generally achieved in

    Journal: The Journal of General Physiology

    Article Title: The Skeletal L-type Ca2+ Current Is a Major Contributor to Excitation-coupled Ca2+ entry

    doi: 10.1085/jgp.200810105

    Figure Lengend Snippet: Skeletal muscle L-type current is blocked by nonspecific cation channel antagonists. Acute block of L-type currents evoked by step depolarizations to +30 mV by 100 μM Gd 3+ (A), 100 μM La 3+ (B), 100 μM 2-APB (C), and 20 μM SKF 96356 (D). The test pulse was applied after a prepulse protocol (see Materials and methods) ( Adams et al., 1990 ). Results of these experiments and application of DMSO vehicle (0.01%) control experiments are summarized in E. For trivalent cations, maximal block was generally achieved in

    Article Snippet: 2-APB (Sigma-Aldrich) and SKF 96356 (provided by O. Delbono, Wake Forest University, Winston-Salem, NC) were dissolved in DMSO (Sigma-Aldrich) to make 1-M and 50-mM stock solutions, respectively.

    Techniques: Blocking Assay

    Effects of store-operated calcium channel blockers on cell survival or injuries of normal and hepatic ischemia-reperfusion injuried hepatocytes. A: MTT assay of normal hepatocytes alone or co-cultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB, or 100 μmol/L La 3+ ( n = 4), the cell survival rate is not significantly different ( P > 0.05); B: MTT assay of HIRI hepatocytes alone or cocultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB or 100 μmol/L La 3+ ( n = 4), the average optical absorbance value in hepatocytes pretreated with 100 μmol/L 2-APB (0.79 ± 0.05) was higher than in untreated hepatocytes (0.69 ± 0.04), a P = 0.027; C: Compared with normal hepatocytes, HIRI hepatocytes incurred cell damage ( n = 4), a P = 0.029. HIRI: Hepatic ischemia-reperfusion injury; 2-APB: 2-aminoethoxydiphenyl borate; DMSO: Dimethylsulfoxide; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Effects and mechanisms of store-operated calcium channel blockade on hepatic ischemia-reperfusion injury in rats

    doi: 10.3748/wjg.v18.i4.356

    Figure Lengend Snippet: Effects of store-operated calcium channel blockers on cell survival or injuries of normal and hepatic ischemia-reperfusion injuried hepatocytes. A: MTT assay of normal hepatocytes alone or co-cultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB, or 100 μmol/L La 3+ ( n = 4), the cell survival rate is not significantly different ( P > 0.05); B: MTT assay of HIRI hepatocytes alone or cocultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB or 100 μmol/L La 3+ ( n = 4), the average optical absorbance value in hepatocytes pretreated with 100 μmol/L 2-APB (0.79 ± 0.05) was higher than in untreated hepatocytes (0.69 ± 0.04), a P = 0.027; C: Compared with normal hepatocytes, HIRI hepatocytes incurred cell damage ( n = 4), a P = 0.029. HIRI: Hepatic ischemia-reperfusion injury; 2-APB: 2-aminoethoxydiphenyl borate; DMSO: Dimethylsulfoxide; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

    Article Snippet: Vasopressin, noradrenalin, thapsigargin (TG), and 2-aminoethoxydiphenyl borate (2-APB) were obtained from Merck KcaA (Darmstadt, Germany).

    Techniques: MTT Assay

    Agonist-induced Ca 2+ oscillations are inhibited by blocking Ca 2+ entry in freshly isolated rat hepatocytes. Traces show Ca 2+ oscillations in freshly isolated rat hepatocytes, with the abscissa axis as time (min), the vertical axis as Fluo-4 fluorescence intensity (∆F/F0), which demonstrates the changes of [Ca 2+ ] i . The number of hepatocytes measured is indicated by “ n ”. A: The fluorescence image (left) and microscopic image (right) of freshly isolated hepatocytes loaded with fluo-4/acetoxymethyl. Ca 2+ oscillations were initiated by 100 nmol/L noradrenaline in the presence (B, n = 27) or absence (C, n = 16) of extracellular Ca 2+ . Ca 2+ oscillations induced by 0.5 nmol/L adenosine-triphosphate were inhibited by 20 μmol/L 2-APB in the presence (D, n = 14) or absence (E, n = 11) of extracellular Ca 2+ . 2-APB: 2-aminoethoxydiphenyl borate.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Effects and mechanisms of store-operated calcium channel blockade on hepatic ischemia-reperfusion injury in rats

    doi: 10.3748/wjg.v18.i4.356

    Figure Lengend Snippet: Agonist-induced Ca 2+ oscillations are inhibited by blocking Ca 2+ entry in freshly isolated rat hepatocytes. Traces show Ca 2+ oscillations in freshly isolated rat hepatocytes, with the abscissa axis as time (min), the vertical axis as Fluo-4 fluorescence intensity (∆F/F0), which demonstrates the changes of [Ca 2+ ] i . The number of hepatocytes measured is indicated by “ n ”. A: The fluorescence image (left) and microscopic image (right) of freshly isolated hepatocytes loaded with fluo-4/acetoxymethyl. Ca 2+ oscillations were initiated by 100 nmol/L noradrenaline in the presence (B, n = 27) or absence (C, n = 16) of extracellular Ca 2+ . Ca 2+ oscillations induced by 0.5 nmol/L adenosine-triphosphate were inhibited by 20 μmol/L 2-APB in the presence (D, n = 14) or absence (E, n = 11) of extracellular Ca 2+ . 2-APB: 2-aminoethoxydiphenyl borate.

    Article Snippet: Vasopressin, noradrenalin, thapsigargin (TG), and 2-aminoethoxydiphenyl borate (2-APB) were obtained from Merck KcaA (Darmstadt, Germany).

    Techniques: Blocking Assay, Isolation, Fluorescence

    IP 3 -mediated calcium signaling participates in fractalkine-induced [Ca 2+ ] i release in BV-2 cells. ( A ) [Ca 2+ ] i was increased by fractalkine in the media with calcium. ( B ) [Ca 2+ ] i was increased by fractalkine in the media with calcium-free. ( C ) The elevation of [Ca 2+ ] i by fractalkine was inhibited by 2-APB. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: IP3-Mediated Calcium Signaling Is Involved in the Mechanism of Fractalkine-Induced Hyperalgesia Response

    doi: 10.12659/MSM.913787

    Figure Lengend Snippet: IP 3 -mediated calcium signaling participates in fractalkine-induced [Ca 2+ ] i release in BV-2 cells. ( A ) [Ca 2+ ] i was increased by fractalkine in the media with calcium. ( B ) [Ca 2+ ] i was increased by fractalkine in the media with calcium-free. ( C ) The elevation of [Ca 2+ ] i by fractalkine was inhibited by 2-APB. * P

    Article Snippet: Pretreatment with anti-CX3CR1, 2-APB, or SB203580 downregulated fractalkine-induced IL-1β and TNF-α secretions at 6, 12, and 24 h ( ).

    Techniques:

    Fractalkine injection lead to thermal hyperalgesia and activated microglia in vivo . ( A ) The thermal nociceptive threshold of mice after receiving fractalkine, 2-APB, antiCX3CR1, and SB203580. ( B ) The immunofluorescence of FKN (fractalkine), Iba-1, and GFAP in mice brain tissues without or with fractalkine. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: IP3-Mediated Calcium Signaling Is Involved in the Mechanism of Fractalkine-Induced Hyperalgesia Response

    doi: 10.12659/MSM.913787

    Figure Lengend Snippet: Fractalkine injection lead to thermal hyperalgesia and activated microglia in vivo . ( A ) The thermal nociceptive threshold of mice after receiving fractalkine, 2-APB, antiCX3CR1, and SB203580. ( B ) The immunofluorescence of FKN (fractalkine), Iba-1, and GFAP in mice brain tissues without or with fractalkine. * P

    Article Snippet: Pretreatment with anti-CX3CR1, 2-APB, or SB203580 downregulated fractalkine-induced IL-1β and TNF-α secretions at 6, 12, and 24 h ( ).

    Techniques: Injection, In Vivo, Mouse Assay, Immunofluorescence

    Fractalkine upregulated pro-inflammatory cytokines in vivo. ( A, B ) The increase of IL-1β and TNF-α by treatment with fractalkine in RT-PCR analysis. ( C, D ) The increase of IL-1β and TNF-α by treatment with fractalkine in ELISA analysis. ( E, F ) The decrease of IL-1β and TNF-α by treatment with anti-CX3CR1, 2-APB, and SB203580. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: IP3-Mediated Calcium Signaling Is Involved in the Mechanism of Fractalkine-Induced Hyperalgesia Response

    doi: 10.12659/MSM.913787

    Figure Lengend Snippet: Fractalkine upregulated pro-inflammatory cytokines in vivo. ( A, B ) The increase of IL-1β and TNF-α by treatment with fractalkine in RT-PCR analysis. ( C, D ) The increase of IL-1β and TNF-α by treatment with fractalkine in ELISA analysis. ( E, F ) The decrease of IL-1β and TNF-α by treatment with anti-CX3CR1, 2-APB, and SB203580. * P

    Article Snippet: Pretreatment with anti-CX3CR1, 2-APB, or SB203580 downregulated fractalkine-induced IL-1β and TNF-α secretions at 6, 12, and 24 h ( ).

    Techniques: In Vivo, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Fractalkine upregulated p-p38MAPK protein in vivo. ( A ) The p-p38MAPK protein was increased after treatment with fractalkine. ( B ) The p-p38MAPK protein was attenuated by pretreatment with anti-CX3CR1, 2-APB, and SB203580. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: IP3-Mediated Calcium Signaling Is Involved in the Mechanism of Fractalkine-Induced Hyperalgesia Response

    doi: 10.12659/MSM.913787

    Figure Lengend Snippet: Fractalkine upregulated p-p38MAPK protein in vivo. ( A ) The p-p38MAPK protein was increased after treatment with fractalkine. ( B ) The p-p38MAPK protein was attenuated by pretreatment with anti-CX3CR1, 2-APB, and SB203580. * P

    Article Snippet: Pretreatment with anti-CX3CR1, 2-APB, or SB203580 downregulated fractalkine-induced IL-1β and TNF-α secretions at 6, 12, and 24 h ( ).

    Techniques: In Vivo

    IP 3 -mediated calcium signaling is involved in fractalkine-induced inflammatory responses in vitro. ( A, B ) The increased of IL-1β and TNF-α by exposed to fractalkine persistently. ( C, D ) The increased of IL-1β and TNF-α was decreased by 2-APB. ( E, F ) The mRNA of IL-1β and TNF-α were increased by exposed to fractalkine persistently. ( G, H ) The mRNA of IL-1β and TNF-α were decreased by 2-APB. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: IP3-Mediated Calcium Signaling Is Involved in the Mechanism of Fractalkine-Induced Hyperalgesia Response

    doi: 10.12659/MSM.913787

    Figure Lengend Snippet: IP 3 -mediated calcium signaling is involved in fractalkine-induced inflammatory responses in vitro. ( A, B ) The increased of IL-1β and TNF-α by exposed to fractalkine persistently. ( C, D ) The increased of IL-1β and TNF-α was decreased by 2-APB. ( E, F ) The mRNA of IL-1β and TNF-α were increased by exposed to fractalkine persistently. ( G, H ) The mRNA of IL-1β and TNF-α were decreased by 2-APB. * P

    Article Snippet: Pretreatment with anti-CX3CR1, 2-APB, or SB203580 downregulated fractalkine-induced IL-1β and TNF-α secretions at 6, 12, and 24 h ( ).

    Techniques: In Vitro

    Involvement of intracellular calcium stores in the mechanism of action of PACAP38 in cerebellar granule cells . (A–D) Effect of a single application of 10 −6 M PACAP38 on the amplitude (A,C) and the area under the curve (B,D) of the [Ca 2+ ] i response in the absence (black bars) or presence (white bars) of 10 −6 M thapsigargin, a Ca 2+ ATPase inhibitor, or 10 −5 M 2APB, a membrane permeable d -myo-inositol 1,4,5-trisphosphate receptor antagonist. Thapsigargin and 2APB were added 15 and 30 min before application of the pulse of PACAP, respectively. (A,C) Histograms showing the distribution of granule cells according to the amplitude of their response to PACAP. Each graph represents the analysis of a least 50 cells from 3 different cultures. 2APB, 2-aminoethoxydiphenyl borate; AUC, area under the curve. *** p

    Journal: Frontiers in Endocrinology

    Article Title: Activation of PAC1 Receptors in Rat Cerebellar Granule Cells Stimulates Both Calcium Mobilization from Intracellular Stores and Calcium Influx through N-Type Calcium Channels

    doi: 10.3389/fendo.2013.00056

    Figure Lengend Snippet: Involvement of intracellular calcium stores in the mechanism of action of PACAP38 in cerebellar granule cells . (A–D) Effect of a single application of 10 −6 M PACAP38 on the amplitude (A,C) and the area under the curve (B,D) of the [Ca 2+ ] i response in the absence (black bars) or presence (white bars) of 10 −6 M thapsigargin, a Ca 2+ ATPase inhibitor, or 10 −5 M 2APB, a membrane permeable d -myo-inositol 1,4,5-trisphosphate receptor antagonist. Thapsigargin and 2APB were added 15 and 30 min before application of the pulse of PACAP, respectively. (A,C) Histograms showing the distribution of granule cells according to the amplitude of their response to PACAP. Each graph represents the analysis of a least 50 cells from 3 different cultures. 2APB, 2-aminoethoxydiphenyl borate; AUC, area under the curve. *** p

    Article Snippet: 2-aminoethoxydiphenyl borate (2APB) was from Fisher Scientific (Illkirch, France).

    Techniques:

    Ca 2+ wave frequency with and without ET-1 (1 nM) in arteries from IH rats in the absence and presence of the nonspecific IP 3 R inhibitor 2-aminoethoxydiphenyl borate (2-APB; 50 μM) and the phospholipase C inhibitor U-73122 (1 μM). Values

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Endothelin-1-induced vasoconstriction does not require intracellular Ca2+ waves in arteries from rats exposed to intermittent hypoxia

    doi: 10.1152/ajpheart.00643.2013

    Figure Lengend Snippet: Ca 2+ wave frequency with and without ET-1 (1 nM) in arteries from IH rats in the absence and presence of the nonspecific IP 3 R inhibitor 2-aminoethoxydiphenyl borate (2-APB; 50 μM) and the phospholipase C inhibitor U-73122 (1 μM). Values

    Article Snippet: Paired wave measurements were also conducted in arteries pretreated with 2-aminoethoxydiphenyl borate (2-APB; 50 μM, Enzo Life Sciences) or U-73122 (1 μM, Tocris Bioscience), inhibitors of IP3 Rs and PLC, respectively.

    Techniques:

    Knockdown of STIM1 attenuates SOCE in human NPCs. (A,B) Ca 2+ -responses during ER-store release and SOCE induced by Thapsigargin (TG, 10 μM) measured using the ratiometric Ca 2+ -indicator indo-1-AM in wild-type (WT) hNPCs (A) or hNPCs treated with pharmacological inhibitors of SOCE, BTP-2 and 2-APB at the indicated concentrations or DMSO as a solvent control (B) . Each trace represents the mean ±SEM for 25–100 cells. Ionomycin (Iono, 10 μM) was added at the end of each imaging to determine the peak F405/485 ratio obtained after saturation of the Ca 2+ -indicator with Ca 2+ . (C) Box plots quantifying the peak F405/485 values for store-release and SOCE in the indicated treatment conditions. Mann–Whitney U test with Bonferroni correction. p = 1.819 × 10 -23 for DMSO control compared to BTP-2 treatment and p = 1.442 × 10 -45 for DMSO control compared to 2-APB treatment. (D) (Top) A representative Western blot showing levels of STIM1 protein in hNPCs transduced with an NTC (non-targeting control) or an sh-RNA targeting STIM1 ( STIM1 KD). Actin serves as the loading control. (Bottom) Quantification of STIM1 band intensities normalized to the loading control Actin from three independent biological replicates ( p = 0.00069, Student’s t -test). (E) Ca 2+ -responses during store-release and SOCE in hNPCs transduced with NTC and STIM1 KD. (F) Box plots quantifying the peak F405/485 values for store-release and SOCE in the indicated genotypes. Peak F405/485 for store-release were not significantly different between NTC and STIM1 KD NPCs. p = 0.0001 for peak F405/485 during SOCE compared between NTC- and STIM1 KD NPCs. (G) Quantification of basal cytosolic [Ca 2+ ] values using Fura-2-AM in NTC- and STIM1 KD NPCs ( p = 1.115 × 10 -8 . Mann–Whitney U test. ( ∗∗∗ Indicates p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Stable STIM1 Knockdown in Self-Renewing Human Neural Precursors Promotes Premature Neural Differentiation

    doi: 10.3389/fnmol.2018.00178

    Figure Lengend Snippet: Knockdown of STIM1 attenuates SOCE in human NPCs. (A,B) Ca 2+ -responses during ER-store release and SOCE induced by Thapsigargin (TG, 10 μM) measured using the ratiometric Ca 2+ -indicator indo-1-AM in wild-type (WT) hNPCs (A) or hNPCs treated with pharmacological inhibitors of SOCE, BTP-2 and 2-APB at the indicated concentrations or DMSO as a solvent control (B) . Each trace represents the mean ±SEM for 25–100 cells. Ionomycin (Iono, 10 μM) was added at the end of each imaging to determine the peak F405/485 ratio obtained after saturation of the Ca 2+ -indicator with Ca 2+ . (C) Box plots quantifying the peak F405/485 values for store-release and SOCE in the indicated treatment conditions. Mann–Whitney U test with Bonferroni correction. p = 1.819 × 10 -23 for DMSO control compared to BTP-2 treatment and p = 1.442 × 10 -45 for DMSO control compared to 2-APB treatment. (D) (Top) A representative Western blot showing levels of STIM1 protein in hNPCs transduced with an NTC (non-targeting control) or an sh-RNA targeting STIM1 ( STIM1 KD). Actin serves as the loading control. (Bottom) Quantification of STIM1 band intensities normalized to the loading control Actin from three independent biological replicates ( p = 0.00069, Student’s t -test). (E) Ca 2+ -responses during store-release and SOCE in hNPCs transduced with NTC and STIM1 KD. (F) Box plots quantifying the peak F405/485 values for store-release and SOCE in the indicated genotypes. Peak F405/485 for store-release were not significantly different between NTC and STIM1 KD NPCs. p = 0.0001 for peak F405/485 during SOCE compared between NTC- and STIM1 KD NPCs. (G) Quantification of basal cytosolic [Ca 2+ ] values using Fura-2-AM in NTC- and STIM1 KD NPCs ( p = 1.115 × 10 -8 . Mann–Whitney U test. ( ∗∗∗ Indicates p

    Article Snippet: To check for inhibition of SOCE, inhibitors of the SOC-channel, BTP-2 (Invitrogen) and 2-APB (Invitrogen) or DMSO (solvent control) were dissolved in the indicated concentration in 100 μl of the ‘0 Ca2+ HBSS.’ For these experiments, the culture medium in which the cells were kept after washing was replaced by HBSS containing either the inhibitors or DMSO and imaged.

    Techniques: Imaging, MANN-WHITNEY, Western Blot, Transduction