2 mercaptoethanol Millipore Search Results


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  • 95
    Millipore penicillin streptomycin
    Penicillin Streptomycin, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 29676 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher 2 mercaptoethanol
    2 Mercaptoethanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 14088 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 2 mercaptoethanol 2 me
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    2 Mercaptoethanol 2 Me, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 556 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Merck KGaA 2 mercaptoethanol
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    2 Mercaptoethanol, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 97/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore 0 05m 2 mercaptoethanol 2 me
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    0 05m 2 Mercaptoethanol 2 Me, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore beta mercaptoethanol 2 me
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    Beta Mercaptoethanol 2 Me, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore orthophthalaldehyde 2 mercaptoethanol derivatizing reagent
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    Orthophthalaldehyde 2 Mercaptoethanol Derivatizing Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore 2 mercaptoethanol cat no 3148 sigma
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    2 Mercaptoethanol Cat No 3148 Sigma, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore 5x10 6 m β mercaptoethanol 2 me
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    5x10 6 M β Mercaptoethanol 2 Me, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore ß mercapthanol 2 me
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    ß Mercapthanol 2 Me, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore 2 mercaptoethanol andpenicillin streptomycin
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    2 Mercaptoethanol Andpenicillin Streptomycin, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore embryomax es cell qualified 2 mercaptoethanol 100x
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    Embryomax Es Cell Qualified 2 Mercaptoethanol 100x, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore appropriate controls 2 me
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    Appropriate Controls 2 Me, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore 2 me s adp
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    2 Me S Adp, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore ß2 mercaptoethanol
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    ß2 Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore β2 mercaptoethanol
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    β2 Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore ofβ mercaptoethanol
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    Ofβ Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Millipore mm2 mercaptoethanol
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    Mm2 Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Millipore 2 melchaptethanol 2 me
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    2 Melchaptethanol 2 Me, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore 1×β mercaptoethanol
    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM <t>mercaptoethanol,</t> pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled
    1×β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore hif 1α inhibitor 2 me
    <t>HIF-1α</t> and miR-21 were induced by hypoxia and play an anti-apoptosis role in intestinal epithelial cells. ( A ) Western blot analysis of HIF-1α expression in response to normoxia, hypoxia, and reoxygenation. ( B ) qRT-PCR analysis of miR-21 expression using U6 snRNA as an internal control. ( C ) Western blot assessment of HIF-1α expression in cells transfected with siRNA against HIF-1α or scrambled control. β-actin served as a loading control. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . ( D ) qRT-PCR analysis of miR-21 expression. U6 snRNA served as an internal control. ** P
    Hif 1α Inhibitor 2 Me, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore b mercaptoethanol 2x laemmli buffer
    <t>HIF-1α</t> and miR-21 were induced by hypoxia and play an anti-apoptosis role in intestinal epithelial cells. ( A ) Western blot analysis of HIF-1α expression in response to normoxia, hypoxia, and reoxygenation. ( B ) qRT-PCR analysis of miR-21 expression using U6 snRNA as an internal control. ( C ) Western blot assessment of HIF-1α expression in cells transfected with siRNA against HIF-1α or scrambled control. β-actin served as a loading control. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . ( D ) qRT-PCR analysis of miR-21 expression. U6 snRNA served as an internal control. ** P
    B Mercaptoethanol 2x Laemmli Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore 2 methoxyestradiol
    Estradiol 17β (E2) and <t>2-methoxyestradiol</t> (2-ME) decreased signal transducer and activator of transcription 5a (STAT5a) but not STAT5b protein levels and inhibited trafficking of the G glycoprotein of the vesicular stomatitis virus (VSV-G)–green
    2 Methoxyestradiol, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore ãŽâ² mercaptoethanol ãŽâ² me
    Estradiol 17β (E2) and <t>2-methoxyestradiol</t> (2-ME) decreased signal transducer and activator of transcription 5a (STAT5a) but not STAT5b protein levels and inhibited trafficking of the G glycoprotein of the vesicular stomatitis virus (VSV-G)–green
    ãŽâ² Mercaptoethanol ãŽâ² Me, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Millipore b2 mercaptoethanol
    Estradiol 17β (E2) and <t>2-methoxyestradiol</t> (2-ME) decreased signal transducer and activator of transcription 5a (STAT5a) but not STAT5b protein levels and inhibited trafficking of the G glycoprotein of the vesicular stomatitis virus (VSV-G)–green
    B2 Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore mercaptoetanol
    Estradiol 17β (E2) and <t>2-methoxyestradiol</t> (2-ME) decreased signal transducer and activator of transcription 5a (STAT5a) but not STAT5b protein levels and inhibited trafficking of the G glycoprotein of the vesicular stomatitis virus (VSV-G)–green
    Mercaptoetanol, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore β mercaptoethenol
    Estradiol 17β (E2) and <t>2-methoxyestradiol</t> (2-ME) decreased signal transducer and activator of transcription 5a (STAT5a) but not STAT5b protein levels and inhibited trafficking of the G glycoprotein of the vesicular stomatitis virus (VSV-G)–green
    β Mercaptoethenol, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β merchaptoethanol
    Estradiol 17β (E2) and <t>2-methoxyestradiol</t> (2-ME) decreased signal transducer and activator of transcription 5a (STAT5a) but not STAT5b protein levels and inhibited trafficking of the G glycoprotein of the vesicular stomatitis virus (VSV-G)–green
    β Merchaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptorthanol
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    91
    Millipore β mercapthoethanol
    Estradiol 17β (E2) and <t>2-methoxyestradiol</t> (2-ME) decreased signal transducer and activator of transcription 5a (STAT5a) but not STAT5b protein levels and inhibited trafficking of the G glycoprotein of the vesicular stomatitis virus (VSV-G)–green
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    Image Search Results


    2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM mercaptoethanol, pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled

    Journal: Biochemistry

    Article Title: Elucidation of IP6 and Heparin Interaction Sites and Conformational Changes in Arrestin-1 by Solution NMR †

    doi: 10.1021/bi101596g

    Figure Lengend Snippet: 2D 1 H- 15 N TROSY spectrum of 0.2mM selectively 15 N-isoleucine-labeled arrestin-1 in 25 mM Bis-Tris, 150mM NaCl and 5mM mercaptoethanol, pH=6.5 at 308 K using a Bruker Avance 800MHz spectrometer. 11 out of 20 isoleucine residue peaks were assigned, as labeled

    Article Snippet: Mercaptoethanol was removed by buffer exchange using a molecular-weight-cut-off at 30 kDa Millipore centrifugal filter.

    Techniques: Labeling

    2-D 1 H- 15 N TROSY spectrum of 0.2mM U- 2 H, 15 N-arrestin-1 in 25 mM Bis-Tris, 150 mM NaCl, 5 mM mercaptoethanol, pH=6.5 acquired at 308 K using a Bruker Avance 800MHz spectrometer. 152 assigned residues are labeled.

    Journal: Biochemistry

    Article Title: Elucidation of IP6 and Heparin Interaction Sites and Conformational Changes in Arrestin-1 by Solution NMR †

    doi: 10.1021/bi101596g

    Figure Lengend Snippet: 2-D 1 H- 15 N TROSY spectrum of 0.2mM U- 2 H, 15 N-arrestin-1 in 25 mM Bis-Tris, 150 mM NaCl, 5 mM mercaptoethanol, pH=6.5 acquired at 308 K using a Bruker Avance 800MHz spectrometer. 152 assigned residues are labeled.

    Article Snippet: Mercaptoethanol was removed by buffer exchange using a molecular-weight-cut-off at 30 kDa Millipore centrifugal filter.

    Techniques: Labeling

    Status of the iron-sulfur cluster in Apo-1, Apo-2 and holo-PfRd. Panel a : Near-UV CD spectra of (native) holo-PfRd (black), Apo-1 PfRd (blue) and Apo-2 PfRd (red), showing the presence or absence of the iron-sulfur cluster-derived CD signatures between 300 nm and 700 nm. The features below 300 nm owe to other structural features (principally the aromatic residues). Panel b : Apo-2 PfRd's absorption spectrum, lacking the iron-sulfur cluster-derived absorption signatures at 390 nm and 490 nm. Panel c : Holo-PfRd's absorption spectrum showing iron-sulfur cluster-derived absorption signatures at 390 nm and 490 nm. Panel d : Apo-2 PfRd (red) releases no iron for uptake by ferrozine, following TCA treatment. In contrast, holo-PfRd releases iron (black), evident from the absorption band peaking at 560 nm, diagnostic of binding of ferrous iron by ferrozine. All released iron is reduced by beta-mercaptoethanol to ferrous form before ferrozine binding.

    Journal: PLoS ONE

    Article Title: The Key to the Extraordinary Thermal Stability of P. furiosus Holo-Rubredoxin: Iron Binding-Guided Packing of a Core Aromatic Cluster Responsible for High Kinetic Stability of the Native Structure

    doi: 10.1371/journal.pone.0089703

    Figure Lengend Snippet: Status of the iron-sulfur cluster in Apo-1, Apo-2 and holo-PfRd. Panel a : Near-UV CD spectra of (native) holo-PfRd (black), Apo-1 PfRd (blue) and Apo-2 PfRd (red), showing the presence or absence of the iron-sulfur cluster-derived CD signatures between 300 nm and 700 nm. The features below 300 nm owe to other structural features (principally the aromatic residues). Panel b : Apo-2 PfRd's absorption spectrum, lacking the iron-sulfur cluster-derived absorption signatures at 390 nm and 490 nm. Panel c : Holo-PfRd's absorption spectrum showing iron-sulfur cluster-derived absorption signatures at 390 nm and 490 nm. Panel d : Apo-2 PfRd (red) releases no iron for uptake by ferrozine, following TCA treatment. In contrast, holo-PfRd releases iron (black), evident from the absorption band peaking at 560 nm, diagnostic of binding of ferrous iron by ferrozine. All released iron is reduced by beta-mercaptoethanol to ferrous form before ferrozine binding.

    Article Snippet: Stock solutions Ferrozine was obtained from Sigma (catalog no. P9762); Fe2+ iron was obtained through dissociation of FeSO4.7H2O obtained from Merck (catalog no. 61751005001730); guanidine hydrochloride (Gdm.HCl) was obtained from Promega (catalog no. H5383); beta-mercaptoethanol was obtained from Sigma (catalog no. M7522).

    Techniques: Derivative Assay, Diagnostic Assay, Binding Assay

    FTIR spectra in the frequencies of amide I vibrations ( a and b ) and FT-Raman spectra ( c – e ) measured at 20°C for 5% ( 1 ), 10% ( 2 ), and 15% ( 3 ) (w/w) Lanreotide acetate in water. The spectra 1′ and 2′ were obtained for Lanreotide concentration similar to the spectra 1 and 2, respectively, but in presence of β -mercaptoethanol (14 M). The spectrum ( 4 ) on d has been recorded for pure β -mercaptoethanol. The spectra 5 and 6 on e have been recorded for pure naphthalene and naphthalene in tetrahydrofurane, respectively.

    Journal: Biophysical Journal

    Article Title: Self-Association Process of a Peptide in Solution: From β-Sheet Filaments to Large Embedded Nanotubes

    doi:

    Figure Lengend Snippet: FTIR spectra in the frequencies of amide I vibrations ( a and b ) and FT-Raman spectra ( c – e ) measured at 20°C for 5% ( 1 ), 10% ( 2 ), and 15% ( 3 ) (w/w) Lanreotide acetate in water. The spectra 1′ and 2′ were obtained for Lanreotide concentration similar to the spectra 1 and 2, respectively, but in presence of β -mercaptoethanol (14 M). The spectrum ( 4 ) on d has been recorded for pure β -mercaptoethanol. The spectra 5 and 6 on e have been recorded for pure naphthalene and naphthalene in tetrahydrofurane, respectively.

    Article Snippet: Glycerol 99.9%, solid naphthalene 99%, and β -mercaptoethanol 14 M were purchased from Sigma (Paris, France).

    Techniques: Concentration Assay

    Preliminary C3b cofactor assay. All samples were reduced with 3% ß-mercaptoethanol in this 8% SDS-PAGE, silver-stained gel. Lane A: 125 ng C3b (containing its α-chain (~101 kD) and ß-chain (~75 kD)). Lane B: 500 ng CFI (with its heavy chain (~50 kD) and light chain (~38 kD)). Lane C: 50 ng CFH (~150 kD). Lane D: 500 ng THP (~100 kD). Lane E: C3b, CFI, and CFH at 37°C for 0 min. Lane F: C3b, CFI, CFH, and THP at 37°C for 0 min. Lane G: same as Lane E except incubated at 37°C for 30 min. Lane H: same as Lane F except incubated at 37°C for 30 min. Starred arrows indicate the degradation products of C3b (C3bα 62 and C3bα 41 ) present in the two samples incubated at 37°C for 30 min.

    Journal: PLoS ONE

    Article Title: Human Tamm-Horsfall protein, a renal specific protein, serves as a cofactor in complement 3b degradation

    doi: 10.1371/journal.pone.0181857

    Figure Lengend Snippet: Preliminary C3b cofactor assay. All samples were reduced with 3% ß-mercaptoethanol in this 8% SDS-PAGE, silver-stained gel. Lane A: 125 ng C3b (containing its α-chain (~101 kD) and ß-chain (~75 kD)). Lane B: 500 ng CFI (with its heavy chain (~50 kD) and light chain (~38 kD)). Lane C: 50 ng CFH (~150 kD). Lane D: 500 ng THP (~100 kD). Lane E: C3b, CFI, and CFH at 37°C for 0 min. Lane F: C3b, CFI, CFH, and THP at 37°C for 0 min. Lane G: same as Lane E except incubated at 37°C for 30 min. Lane H: same as Lane F except incubated at 37°C for 30 min. Starred arrows indicate the degradation products of C3b (C3bα 62 and C3bα 41 ) present in the two samples incubated at 37°C for 30 min.

    Article Snippet: CFH (1.5 μg/lane) (Calbiochem, San Diego, CA, USA), both non-reduced and reduced (with 3% ß-mercaptoethanol (Sigma Chemical Co., St. Louis, MO, USA)) was electrophoresed on a 7.5% SDS-PAGE gel and then was transferred electrophoretically (70V, 2 h) to nitrocellulose (NC) (Immobilon, Millipore, Bedford, MA, USA).

    Techniques: SDS Page, Staining, Incubation

    Cofactor activity present in samples with THP, but without CFH. Silver-stained 8% SDS-PAGE gel where all samples were reduced with 3% ß-mercaptoethanol. Samples analyzed in all lanes contained 125 ng C3b and 500 ng CFI. Lanes A, B, and D contained 0.5 ng CFH. Lanes C and D contained 5 μg THP. Lanes A, C, and D were incubated at 37°C for 30 min, while Lane B sample was kept at 4°C. Starred arrows indicate the degradation products of C3b.

    Journal: PLoS ONE

    Article Title: Human Tamm-Horsfall protein, a renal specific protein, serves as a cofactor in complement 3b degradation

    doi: 10.1371/journal.pone.0181857

    Figure Lengend Snippet: Cofactor activity present in samples with THP, but without CFH. Silver-stained 8% SDS-PAGE gel where all samples were reduced with 3% ß-mercaptoethanol. Samples analyzed in all lanes contained 125 ng C3b and 500 ng CFI. Lanes A, B, and D contained 0.5 ng CFH. Lanes C and D contained 5 μg THP. Lanes A, C, and D were incubated at 37°C for 30 min, while Lane B sample was kept at 4°C. Starred arrows indicate the degradation products of C3b.

    Article Snippet: CFH (1.5 μg/lane) (Calbiochem, San Diego, CA, USA), both non-reduced and reduced (with 3% ß-mercaptoethanol (Sigma Chemical Co., St. Louis, MO, USA)) was electrophoresed on a 7.5% SDS-PAGE gel and then was transferred electrophoretically (70V, 2 h) to nitrocellulose (NC) (Immobilon, Millipore, Bedford, MA, USA).

    Techniques: Activity Assay, Staining, SDS Page, Incubation

    Analysis of cofactor activity in deglycosylated THP (dTHP). 8% SDS-PAGE silver-stained gel of ß-mercaptoethanol-reduced samples. All lanes were loaded with a mixture that contained 125 ng C3b and 150 ng CFI. Lanes C and D contained 3 μg THP while Lanes E and F contained what was estimated to be about 2 μg dTHP. Samples analyzed in Lanes B, D, and F were incubated at 37°C for 4 h while the other samples were not incubated at 37°C. Starred arrows indicate the degradation products of C3b.

    Journal: PLoS ONE

    Article Title: Human Tamm-Horsfall protein, a renal specific protein, serves as a cofactor in complement 3b degradation

    doi: 10.1371/journal.pone.0181857

    Figure Lengend Snippet: Analysis of cofactor activity in deglycosylated THP (dTHP). 8% SDS-PAGE silver-stained gel of ß-mercaptoethanol-reduced samples. All lanes were loaded with a mixture that contained 125 ng C3b and 150 ng CFI. Lanes C and D contained 3 μg THP while Lanes E and F contained what was estimated to be about 2 μg dTHP. Samples analyzed in Lanes B, D, and F were incubated at 37°C for 4 h while the other samples were not incubated at 37°C. Starred arrows indicate the degradation products of C3b.

    Article Snippet: CFH (1.5 μg/lane) (Calbiochem, San Diego, CA, USA), both non-reduced and reduced (with 3% ß-mercaptoethanol (Sigma Chemical Co., St. Louis, MO, USA)) was electrophoresed on a 7.5% SDS-PAGE gel and then was transferred electrophoretically (70V, 2 h) to nitrocellulose (NC) (Immobilon, Millipore, Bedford, MA, USA).

    Techniques: Activity Assay, SDS Page, Staining, Incubation

    Comparison of cofactor activity in THP samples from 10 different individuals. 8% SDS-PAGE silver-stained gel of ß-mercaptoethanol-reduced samples. All lanes contained 125 ng C3b and 150 ng CFI. Lanes C → G samples contained 5 μg THP from 5 different females. Lanes H → L samples contained 5 μg THP from 5 different males. Samples in Lanes B → L were incubated at 37°C for 4 h. The sample in Lane A was not incubated at 37°C and was used to compare with the 37°C incubated sample in Lane B, both lanes containing only C3b and CFI. Starred arrows indicate the C3bα 62 degradation product of C3b.

    Journal: PLoS ONE

    Article Title: Human Tamm-Horsfall protein, a renal specific protein, serves as a cofactor in complement 3b degradation

    doi: 10.1371/journal.pone.0181857

    Figure Lengend Snippet: Comparison of cofactor activity in THP samples from 10 different individuals. 8% SDS-PAGE silver-stained gel of ß-mercaptoethanol-reduced samples. All lanes contained 125 ng C3b and 150 ng CFI. Lanes C → G samples contained 5 μg THP from 5 different females. Lanes H → L samples contained 5 μg THP from 5 different males. Samples in Lanes B → L were incubated at 37°C for 4 h. The sample in Lane A was not incubated at 37°C and was used to compare with the 37°C incubated sample in Lane B, both lanes containing only C3b and CFI. Starred arrows indicate the C3bα 62 degradation product of C3b.

    Article Snippet: CFH (1.5 μg/lane) (Calbiochem, San Diego, CA, USA), both non-reduced and reduced (with 3% ß-mercaptoethanol (Sigma Chemical Co., St. Louis, MO, USA)) was electrophoresed on a 7.5% SDS-PAGE gel and then was transferred electrophoretically (70V, 2 h) to nitrocellulose (NC) (Immobilon, Millipore, Bedford, MA, USA).

    Techniques: Activity Assay, SDS Page, Staining, Incubation

    Dose effect of THP concentration on cofactor activity. (A) 8% SDS-PAGE silver-stained gel of ß-mercaptoethanol-reduced samples. All lanes were loaded with 125 ng C3b and 150 ng CFI. Samples in all lanes except that of Lane A were incubated at 37°C for 4 h. Lanes C → I contained samples with varying concentrations of a male THP sample (from 10 μg to 0.25 μg). Starred arrows indicate the degradation products of C3b. (B) Plot of THP quantity versus intensity of band ratio obtained after Image J analysis of the intensity of the C3bα 62 fragment versus the C3b ß-chain in each lane minus the background cleavage present in Lane B.

    Journal: PLoS ONE

    Article Title: Human Tamm-Horsfall protein, a renal specific protein, serves as a cofactor in complement 3b degradation

    doi: 10.1371/journal.pone.0181857

    Figure Lengend Snippet: Dose effect of THP concentration on cofactor activity. (A) 8% SDS-PAGE silver-stained gel of ß-mercaptoethanol-reduced samples. All lanes were loaded with 125 ng C3b and 150 ng CFI. Samples in all lanes except that of Lane A were incubated at 37°C for 4 h. Lanes C → I contained samples with varying concentrations of a male THP sample (from 10 μg to 0.25 μg). Starred arrows indicate the degradation products of C3b. (B) Plot of THP quantity versus intensity of band ratio obtained after Image J analysis of the intensity of the C3bα 62 fragment versus the C3b ß-chain in each lane minus the background cleavage present in Lane B.

    Article Snippet: CFH (1.5 μg/lane) (Calbiochem, San Diego, CA, USA), both non-reduced and reduced (with 3% ß-mercaptoethanol (Sigma Chemical Co., St. Louis, MO, USA)) was electrophoresed on a 7.5% SDS-PAGE gel and then was transferred electrophoretically (70V, 2 h) to nitrocellulose (NC) (Immobilon, Millipore, Bedford, MA, USA).

    Techniques: Concentration Assay, Activity Assay, SDS Page, Staining, Incubation

    HIF-1α and miR-21 were induced by hypoxia and play an anti-apoptosis role in intestinal epithelial cells. ( A ) Western blot analysis of HIF-1α expression in response to normoxia, hypoxia, and reoxygenation. ( B ) qRT-PCR analysis of miR-21 expression using U6 snRNA as an internal control. ( C ) Western blot assessment of HIF-1α expression in cells transfected with siRNA against HIF-1α or scrambled control. β-actin served as a loading control. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . ( D ) qRT-PCR analysis of miR-21 expression. U6 snRNA served as an internal control. ** P

    Journal: Scientific Reports

    Article Title: Ischemic Postconditioning Protects Against Intestinal Ischemia/Reperfusion Injury via the HIF-1α/miR-21 Axis

    doi: 10.1038/s41598-017-16366-6

    Figure Lengend Snippet: HIF-1α and miR-21 were induced by hypoxia and play an anti-apoptosis role in intestinal epithelial cells. ( A ) Western blot analysis of HIF-1α expression in response to normoxia, hypoxia, and reoxygenation. ( B ) qRT-PCR analysis of miR-21 expression using U6 snRNA as an internal control. ( C ) Western blot assessment of HIF-1α expression in cells transfected with siRNA against HIF-1α or scrambled control. β-actin served as a loading control. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . ( D ) qRT-PCR analysis of miR-21 expression. U6 snRNA served as an internal control. ** P

    Article Snippet: Inhibition of HIF-1α in the rat model The HIF-1α inhibitor 2-ME (Sigma-Aldrich; St. Louis, MO, USA) was dissolved in DMSO (2%, Sigma-Aldrich) and diluted with arachis oil.

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Transfection

    HIF-1α inhibition downregulated miR-21 and abolished the protective effects of IPostC. ( A ) Western blot analysis of HIF-1α expression in the nucleus. ( B ) qRT-PCR analysis of miR-21 expression using U6 snRNA as an internal control. ( C and D ) Representative photomicrographs of HE-stained intestinal sections and intestinal injury score. Magnification × 200. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . Data are expressed as mean ± SD, n = 6. ** P

    Journal: Scientific Reports

    Article Title: Ischemic Postconditioning Protects Against Intestinal Ischemia/Reperfusion Injury via the HIF-1α/miR-21 Axis

    doi: 10.1038/s41598-017-16366-6

    Figure Lengend Snippet: HIF-1α inhibition downregulated miR-21 and abolished the protective effects of IPostC. ( A ) Western blot analysis of HIF-1α expression in the nucleus. ( B ) qRT-PCR analysis of miR-21 expression using U6 snRNA as an internal control. ( C and D ) Representative photomicrographs of HE-stained intestinal sections and intestinal injury score. Magnification × 200. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . Data are expressed as mean ± SD, n = 6. ** P

    Article Snippet: Inhibition of HIF-1α in the rat model The HIF-1α inhibitor 2-ME (Sigma-Aldrich; St. Louis, MO, USA) was dissolved in DMSO (2%, Sigma-Aldrich) and diluted with arachis oil.

    Techniques: Inhibition, Western Blot, Expressing, Quantitative RT-PCR, Staining

    IPostC attenuated intestinal I/R injury and affected expression of HIF-1α and miR-21. ( A ) Hematoxylin and eosin (HE) staining was performed to analyze intestinal injury. Magnification × 200. ( B ) HE-stained, formalin-fixed tissue sections were scored for intestinal epithelial damage (0–6). ( C and D ) Evaluation of apoptosis by TUNEL staining and measurement of the apoptotic index. Magnification × 200. ( E ) Western blot analysis of HIF-1α expression in intestinal tissues. ( F ) qRT-PCR analysis of miR-21 expression in the indicated groups. U6 snRNA served as an internal control. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . Data are expressed as mean ± SD, n = 6. ** P

    Journal: Scientific Reports

    Article Title: Ischemic Postconditioning Protects Against Intestinal Ischemia/Reperfusion Injury via the HIF-1α/miR-21 Axis

    doi: 10.1038/s41598-017-16366-6

    Figure Lengend Snippet: IPostC attenuated intestinal I/R injury and affected expression of HIF-1α and miR-21. ( A ) Hematoxylin and eosin (HE) staining was performed to analyze intestinal injury. Magnification × 200. ( B ) HE-stained, formalin-fixed tissue sections were scored for intestinal epithelial damage (0–6). ( C and D ) Evaluation of apoptosis by TUNEL staining and measurement of the apoptotic index. Magnification × 200. ( E ) Western blot analysis of HIF-1α expression in intestinal tissues. ( F ) qRT-PCR analysis of miR-21 expression in the indicated groups. U6 snRNA served as an internal control. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . Data are expressed as mean ± SD, n = 6. ** P

    Article Snippet: Inhibition of HIF-1α in the rat model The HIF-1α inhibitor 2-ME (Sigma-Aldrich; St. Louis, MO, USA) was dissolved in DMSO (2%, Sigma-Aldrich) and diluted with arachis oil.

    Techniques: Expressing, Staining, TUNEL Assay, Western Blot, Quantitative RT-PCR

    Inhibition of HIF-1α abolished the IPostC-induced suppression of apoptosis. ( A and B ) TUNEL staining and apoptotic index in the different groups. Magnification ×200. ( C ) Western blot analysis of PDCD4 and Fas-L expression using β-actin as the internal control. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . Data are expressed as mean ± SD. n = 6. ** P

    Journal: Scientific Reports

    Article Title: Ischemic Postconditioning Protects Against Intestinal Ischemia/Reperfusion Injury via the HIF-1α/miR-21 Axis

    doi: 10.1038/s41598-017-16366-6

    Figure Lengend Snippet: Inhibition of HIF-1α abolished the IPostC-induced suppression of apoptosis. ( A and B ) TUNEL staining and apoptotic index in the different groups. Magnification ×200. ( C ) Western blot analysis of PDCD4 and Fas-L expression using β-actin as the internal control. Unprocessed original scans and of blots are shown in Supplementary Fig. S1 . Data are expressed as mean ± SD. n = 6. ** P

    Article Snippet: Inhibition of HIF-1α in the rat model The HIF-1α inhibitor 2-ME (Sigma-Aldrich; St. Louis, MO, USA) was dissolved in DMSO (2%, Sigma-Aldrich) and diluted with arachis oil.

    Techniques: Inhibition, TUNEL Assay, Staining, Western Blot, Expressing

    Estradiol 17β (E2) and 2-methoxyestradiol (2-ME) decreased signal transducer and activator of transcription 5a (STAT5a) but not STAT5b protein levels and inhibited trafficking of the G glycoprotein of the vesicular stomatitis virus (VSV-G)–green

    Journal: Pulmonary Circulation

    Article Title: Subcellular mechanisms in pulmonary arterial hypertension: combinatorial modalities that inhibit anterograde trafficking and cause bone morphogenetic protein receptor type 2 mislocalization

    doi: 10.1086/674336

    Figure Lengend Snippet: Estradiol 17β (E2) and 2-methoxyestradiol (2-ME) decreased signal transducer and activator of transcription 5a (STAT5a) but not STAT5b protein levels and inhibited trafficking of the G glycoprotein of the vesicular stomatitis virus (VSV-G)–green

    Article Snippet: E2 (E2758), 2-methoxyestradiol (2-ME; M6383), and probenecid (P8761) were also from Sigma-Aldrich.

    Techniques: