2 2 azino bis Search Results


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  • 99
    Thermo Fisher 2 azino bis
    2 Azino Bis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 azino bis/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    2 azino bis - by Bioz Stars, 2020-04
    99/100 stars
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    99
    Millipore 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid
    Ecological and biochemical characteristics of the selected fungi. Note: ABTS = <t>2,2′-azino-bis</t> <t>3-ethylbenzothiazoline-6-sulfonic</t> acid; CMC = carboxymethyl cellulose.
    2 2 Azino Bis 3 Ethylbenzothiazoline 6 Sulfonic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid/product/Millipore
    Average 99 stars, based on 37 article reviews
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    2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid - by Bioz Stars, 2020-04
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    94
    Millipore 2 azinobis
    Scatter plot of the PCA of the antioxidant capacity and metabolites determined in the plant material; ( A ) PC1 versus PC2, ( B ) PC1 versus PC3. Different colors describe groups of samples according to the cluster analysis ( Figure 3 ). TRB: callus from roots of plants with tomentose leaves growing on medium B, TLA: callus from leaves of plants with tomentose leaves growing on medium A, TLB: callus from leaves of plants with tomentose leaves growing on medium B, TLC: callus from leaves of plants with tomentose leaves growing on medium C, GLA: callus from leaves of plants with glabrous leaves growing on medium A, GLC: callus from leaves of plants with glabrous leaves growing on medium C, TRC: callus from roots of plants with tomentose leaves growing on medium C, GRB: callus from roots of plants with glabrous leaves growing on medium B, TLE: callus from leaves of plants with tomentose leaves growing on medium E, GRD: callus from roots of plants with glabrous leaves growing on medium D, GRE: callus from roots of plants with glabrous leaves growing on medium E, GLE: callus from leaves of plants with glabrous leaves growing on medium E, TRE: callus from roots of plants with tomentose leaves growing on medium E, TLD: callus from leaves of plants with tomentose leaves, growing on medium D, GRC: callus from roots of plants with glabrous leaves growing on medium C, GLD: callus from leaves of plants with glabrous leaves growing on medium D, SS: shoot of a plant grown in soil culture, RS: root of a plant grown in soil culture, SH: shoot of a plant grown in hydroponic culture, RH: root of a plant grown in hydroponic culture, UA: oleanolic acid, OA: ursolic acid, CO: carlina oxide, 3,5QA: 3,5-Di-caffeoylquinic acid, ChA: chlorogenic acid. FC: flavonoid content, DPPH, ABTS and TPC—antioxidant activity evaluated in tests with 2,2-diphenyl-1-picrylhydrazyl, <t>2-azino-bis</t> <t>(3-ethylbenzthiazoline-6-sulphonic</t> acid and Folin–Ciocalteu reagent, respectively.
    2 Azinobis, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1418 article reviews
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    2 azinobis - by Bioz Stars, 2020-04
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    93
    Roche 2 2 azino bis
    Scatter plot of the PCA of the antioxidant capacity and metabolites determined in the plant material; ( A ) PC1 versus PC2, ( B ) PC1 versus PC3. Different colors describe groups of samples according to the cluster analysis ( Figure 3 ). TRB: callus from roots of plants with tomentose leaves growing on medium B, TLA: callus from leaves of plants with tomentose leaves growing on medium A, TLB: callus from leaves of plants with tomentose leaves growing on medium B, TLC: callus from leaves of plants with tomentose leaves growing on medium C, GLA: callus from leaves of plants with glabrous leaves growing on medium A, GLC: callus from leaves of plants with glabrous leaves growing on medium C, TRC: callus from roots of plants with tomentose leaves growing on medium C, GRB: callus from roots of plants with glabrous leaves growing on medium B, TLE: callus from leaves of plants with tomentose leaves growing on medium E, GRD: callus from roots of plants with glabrous leaves growing on medium D, GRE: callus from roots of plants with glabrous leaves growing on medium E, GLE: callus from leaves of plants with glabrous leaves growing on medium E, TRE: callus from roots of plants with tomentose leaves growing on medium E, TLD: callus from leaves of plants with tomentose leaves, growing on medium D, GRC: callus from roots of plants with glabrous leaves growing on medium C, GLD: callus from leaves of plants with glabrous leaves growing on medium D, SS: shoot of a plant grown in soil culture, RS: root of a plant grown in soil culture, SH: shoot of a plant grown in hydroponic culture, RH: root of a plant grown in hydroponic culture, UA: oleanolic acid, OA: ursolic acid, CO: carlina oxide, 3,5QA: 3,5-Di-caffeoylquinic acid, ChA: chlorogenic acid. FC: flavonoid content, DPPH, ABTS and TPC—antioxidant activity evaluated in tests with 2,2-diphenyl-1-picrylhydrazyl, <t>2-azino-bis</t> <t>(3-ethylbenzthiazoline-6-sulphonic</t> acid and Folin–Ciocalteu reagent, respectively.
    2 2 Azino Bis, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 252 article reviews
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    2 2 azino bis - by Bioz Stars, 2020-04
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    93
    Fluorochem Ltd 2 2 azino bis
    Scatter plot of the PCA of the antioxidant capacity and metabolites determined in the plant material; ( A ) PC1 versus PC2, ( B ) PC1 versus PC3. Different colors describe groups of samples according to the cluster analysis ( Figure 3 ). TRB: callus from roots of plants with tomentose leaves growing on medium B, TLA: callus from leaves of plants with tomentose leaves growing on medium A, TLB: callus from leaves of plants with tomentose leaves growing on medium B, TLC: callus from leaves of plants with tomentose leaves growing on medium C, GLA: callus from leaves of plants with glabrous leaves growing on medium A, GLC: callus from leaves of plants with glabrous leaves growing on medium C, TRC: callus from roots of plants with tomentose leaves growing on medium C, GRB: callus from roots of plants with glabrous leaves growing on medium B, TLE: callus from leaves of plants with tomentose leaves growing on medium E, GRD: callus from roots of plants with glabrous leaves growing on medium D, GRE: callus from roots of plants with glabrous leaves growing on medium E, GLE: callus from leaves of plants with glabrous leaves growing on medium E, TRE: callus from roots of plants with tomentose leaves growing on medium E, TLD: callus from leaves of plants with tomentose leaves, growing on medium D, GRC: callus from roots of plants with glabrous leaves growing on medium C, GLD: callus from leaves of plants with glabrous leaves growing on medium D, SS: shoot of a plant grown in soil culture, RS: root of a plant grown in soil culture, SH: shoot of a plant grown in hydroponic culture, RH: root of a plant grown in hydroponic culture, UA: oleanolic acid, OA: ursolic acid, CO: carlina oxide, 3,5QA: 3,5-Di-caffeoylquinic acid, ChA: chlorogenic acid. FC: flavonoid content, DPPH, ABTS and TPC—antioxidant activity evaluated in tests with 2,2-diphenyl-1-picrylhydrazyl, <t>2-azino-bis</t> <t>(3-ethylbenzthiazoline-6-sulphonic</t> acid and Folin–Ciocalteu reagent, respectively.
    2 2 Azino Bis, supplied by Fluorochem Ltd, used in various techniques. Bioz Stars score: 93/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 2 azino bis/product/Fluorochem Ltd
    Average 93 stars, based on 251 article reviews
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    2 2 azino bis - by Bioz Stars, 2020-04
    93/100 stars
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    86
    SouthernBiotech 2 azino bis
    Scatter plot of the PCA of the antioxidant capacity and metabolites determined in the plant material; ( A ) PC1 versus PC2, ( B ) PC1 versus PC3. Different colors describe groups of samples according to the cluster analysis ( Figure 3 ). TRB: callus from roots of plants with tomentose leaves growing on medium B, TLA: callus from leaves of plants with tomentose leaves growing on medium A, TLB: callus from leaves of plants with tomentose leaves growing on medium B, TLC: callus from leaves of plants with tomentose leaves growing on medium C, GLA: callus from leaves of plants with glabrous leaves growing on medium A, GLC: callus from leaves of plants with glabrous leaves growing on medium C, TRC: callus from roots of plants with tomentose leaves growing on medium C, GRB: callus from roots of plants with glabrous leaves growing on medium B, TLE: callus from leaves of plants with tomentose leaves growing on medium E, GRD: callus from roots of plants with glabrous leaves growing on medium D, GRE: callus from roots of plants with glabrous leaves growing on medium E, GLE: callus from leaves of plants with glabrous leaves growing on medium E, TRE: callus from roots of plants with tomentose leaves growing on medium E, TLD: callus from leaves of plants with tomentose leaves, growing on medium D, GRC: callus from roots of plants with glabrous leaves growing on medium C, GLD: callus from leaves of plants with glabrous leaves growing on medium D, SS: shoot of a plant grown in soil culture, RS: root of a plant grown in soil culture, SH: shoot of a plant grown in hydroponic culture, RH: root of a plant grown in hydroponic culture, UA: oleanolic acid, OA: ursolic acid, CO: carlina oxide, 3,5QA: 3,5-Di-caffeoylquinic acid, ChA: chlorogenic acid. FC: flavonoid content, DPPH, ABTS and TPC—antioxidant activity evaluated in tests with 2,2-diphenyl-1-picrylhydrazyl, <t>2-azino-bis</t> <t>(3-ethylbenzthiazoline-6-sulphonic</t> acid and Folin–Ciocalteu reagent, respectively.
    2 Azino Bis, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 5 article reviews
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    2 azino bis - by Bioz Stars, 2020-04
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    91
    Beyotime 2 azino bis
    CsUGT78A14 confer flavonoids enhanced reactive oxygen species (ROS) scavenging capacity. ROS scavenging activity of reaction products formed by CsUGT78A14-1 and control was compared. Kaempferol (A , C , E) and quercetin (B , D , F) were used as substrates. ROS scavenger capacity of kaempferol and quercetin incubated with CsUGT78A14-1 was significantly enhanced compared to the control by <t>2,2-diphenyl-1-picrylhydrazyl</t> (A , B) , ferric reducing ability of plasma (C , D) , and <t>2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) method (E , F) . Three experimental replicates were conducted for the ROS scavenging capacity analysis. NS, P=0.05141.
    2 Azino Bis, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2 azino bis - by Bioz Stars, 2020-04
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    86
    Perry Laboratory 2 azino bis
    CsUGT78A14 confer flavonoids enhanced reactive oxygen species (ROS) scavenging capacity. ROS scavenging activity of reaction products formed by CsUGT78A14-1 and control was compared. Kaempferol (A , C , E) and quercetin (B , D , F) were used as substrates. ROS scavenger capacity of kaempferol and quercetin incubated with CsUGT78A14-1 was significantly enhanced compared to the control by <t>2,2-diphenyl-1-picrylhydrazyl</t> (A , B) , ferric reducing ability of plasma (C , D) , and <t>2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) method (E , F) . Three experimental replicates were conducted for the ROS scavenging capacity analysis. NS, P=0.05141.
    2 Azino Bis, supplied by Perry Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 6 article reviews
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    2 azino bis - by Bioz Stars, 2020-04
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    91
    Vector Laboratories 2 azino bis
    CsUGT78A14 confer flavonoids enhanced reactive oxygen species (ROS) scavenging capacity. ROS scavenging activity of reaction products formed by CsUGT78A14-1 and control was compared. Kaempferol (A , C , E) and quercetin (B , D , F) were used as substrates. ROS scavenger capacity of kaempferol and quercetin incubated with CsUGT78A14-1 was significantly enhanced compared to the control by <t>2,2-diphenyl-1-picrylhydrazyl</t> (A , B) , ferric reducing ability of plasma (C , D) , and <t>2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) method (E , F) . Three experimental replicates were conducted for the ROS scavenging capacity analysis. NS, P=0.05141.
    2 Azino Bis, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 azino bis/product/Vector Laboratories
    Average 91 stars, based on 1 article reviews
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    2 azino bis - by Bioz Stars, 2020-04
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    92
    Amresco 2 azino bis
    CsUGT78A14 confer flavonoids enhanced reactive oxygen species (ROS) scavenging capacity. ROS scavenging activity of reaction products formed by CsUGT78A14-1 and control was compared. Kaempferol (A , C , E) and quercetin (B , D , F) were used as substrates. ROS scavenger capacity of kaempferol and quercetin incubated with CsUGT78A14-1 was significantly enhanced compared to the control by <t>2,2-diphenyl-1-picrylhydrazyl</t> (A , B) , ferric reducing ability of plasma (C , D) , and <t>2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) method (E , F) . Three experimental replicates were conducted for the ROS scavenging capacity analysis. NS, P=0.05141.
    2 Azino Bis, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Tokyo Chemical Industry 2 azino bis
    Effects of methods of burdock root tea (BRT) preparation on antioxidant activities. In order to elucidate the effects of BRT preparation conditions on antioxidant activity, roasting number and tea extraction time were varied. Burdock roots were roasted 0, 3, or 6 times, and BRT was produced by boiling these roasted burdock roots (1.00 mg/mL) for 30 or 60 min at 100°C. Total antioxidant capacities of BRT were determined based on <t>2,2-diphenyl-1-picrylhydrazyl</t> (DPPH) radical scavenging activity (A), <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Results are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–e) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.
    2 Azino Bis, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 azino bis/product/Tokyo Chemical Industry
    Average 91 stars, based on 1 article reviews
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    2 azino bis - by Bioz Stars, 2020-04
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    85
    Boehringer Mannheim 2 azinobis
    Effects of methods of burdock root tea (BRT) preparation on antioxidant activities. In order to elucidate the effects of BRT preparation conditions on antioxidant activity, roasting number and tea extraction time were varied. Burdock roots were roasted 0, 3, or 6 times, and BRT was produced by boiling these roasted burdock roots (1.00 mg/mL) for 30 or 60 min at 100°C. Total antioxidant capacities of BRT were determined based on <t>2,2-diphenyl-1-picrylhydrazyl</t> (DPPH) radical scavenging activity (A), <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Results are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–e) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.
    2 Azinobis, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    FUJIFILM 2 2 azino bis
    Effects of methods of burdock root tea (BRT) preparation on antioxidant activities. In order to elucidate the effects of BRT preparation conditions on antioxidant activity, roasting number and tea extraction time were varied. Burdock roots were roasted 0, 3, or 6 times, and BRT was produced by boiling these roasted burdock roots (1.00 mg/mL) for 30 or 60 min at 100°C. Total antioxidant capacities of BRT were determined based on <t>2,2-diphenyl-1-picrylhydrazyl</t> (DPPH) radical scavenging activity (A), <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Results are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–e) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.
    2 2 Azino Bis, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 24 article reviews
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    2 2 azino bis - by Bioz Stars, 2020-04
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    94
    Sangon Biotech 2 2 azino bis
    Effects of methods of burdock root tea (BRT) preparation on antioxidant activities. In order to elucidate the effects of BRT preparation conditions on antioxidant activity, roasting number and tea extraction time were varied. Burdock roots were roasted 0, 3, or 6 times, and BRT was produced by boiling these roasted burdock roots (1.00 mg/mL) for 30 or 60 min at 100°C. Total antioxidant capacities of BRT were determined based on <t>2,2-diphenyl-1-picrylhydrazyl</t> (DPPH) radical scavenging activity (A), <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Results are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–e) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.
    2 2 Azino Bis, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 15 article reviews
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    2 2 azino bis - by Bioz Stars, 2020-04
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    93
    Merck KGaA 2 2 azino bis
    Effects of methods of burdock root tea (BRT) preparation on antioxidant activities. In order to elucidate the effects of BRT preparation conditions on antioxidant activity, roasting number and tea extraction time were varied. Burdock roots were roasted 0, 3, or 6 times, and BRT was produced by boiling these roasted burdock roots (1.00 mg/mL) for 30 or 60 min at 100°C. Total antioxidant capacities of BRT were determined based on <t>2,2-diphenyl-1-picrylhydrazyl</t> (DPPH) radical scavenging activity (A), <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Results are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–e) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.
    2 2 Azino Bis, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2 2 azino bis - by Bioz Stars, 2020-04
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    91
    Valiant 2 2 azino bis
    Effects of methods of burdock root tea (BRT) preparation on antioxidant activities. In order to elucidate the effects of BRT preparation conditions on antioxidant activity, roasting number and tea extraction time were varied. Burdock roots were roasted 0, 3, or 6 times, and BRT was produced by boiling these roasted burdock roots (1.00 mg/mL) for 30 or 60 min at 100°C. Total antioxidant capacities of BRT were determined based on <t>2,2-diphenyl-1-picrylhydrazyl</t> (DPPH) radical scavenging activity (A), <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Results are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–e) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.
    2 2 Azino Bis, supplied by Valiant, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2 2 azino bis - by Bioz Stars, 2020-04
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    86
    Bio-Rad 2 2 azino bis
    Effects of methods of burdock root tea (BRT) preparation on antioxidant activities. In order to elucidate the effects of BRT preparation conditions on antioxidant activity, roasting number and tea extraction time were varied. Burdock roots were roasted 0, 3, or 6 times, and BRT was produced by boiling these roasted burdock roots (1.00 mg/mL) for 30 or 60 min at 100°C. Total antioxidant capacities of BRT were determined based on <t>2,2-diphenyl-1-picrylhydrazyl</t> (DPPH) radical scavenging activity (A), <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic</t> acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Results are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–e) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.
    2 2 Azino Bis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 49 article reviews
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    2 2 azino bis - by Bioz Stars, 2020-04
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    91
    Boehringer Mannheim 2 2 azino bis
    Characterization of the aptamers recognizing the Leishmania and human PABP1 proteins . ( a ) Recombinant GST-PABP protein or GST were plated at 500 ng/well and incubated with the digoxigenin-labeled aptamers (10 nmol/l). Anti-digoxigenin-POD antibodies were added for 1 hour and revealed with an <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) solution at 405 nm. All the experiments were performed in triplicate and the average of three different experiments is shown: * P
    2 2 Azino Bis, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 91/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2 2 azino bis - by Bioz Stars, 2020-04
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    93
    Randox 2 2 azino bis
    Characterization of the aptamers recognizing the Leishmania and human PABP1 proteins . ( a ) Recombinant GST-PABP protein or GST were plated at 500 ng/well and incubated with the digoxigenin-labeled aptamers (10 nmol/l). Anti-digoxigenin-POD antibodies were added for 1 hour and revealed with an <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) solution at 405 nm. All the experiments were performed in triplicate and the average of three different experiments is shown: * P
    2 2 Azino Bis, supplied by Randox, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2 2 azino bis - by Bioz Stars, 2020-04
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    93
    Beijing Solarbio Science 2 2 azino bis
    Characterization of the aptamers recognizing the Leishmania and human PABP1 proteins . ( a ) Recombinant GST-PABP protein or GST were plated at 500 ng/well and incubated with the digoxigenin-labeled aptamers (10 nmol/l). Anti-digoxigenin-POD antibodies were added for 1 hour and revealed with an <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) solution at 405 nm. All the experiments were performed in triplicate and the average of three different experiments is shown: * P
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    Free radical scavenging activity of broccoli extract (BE) and Trolox (a water soluble tocopherol analogue as a positive standard) against <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) (ABTS) radical formation. Negative controls were 0 μM BE or 0 μM Trolox. Results are means±SD of five measurements. Values with different letters (a–f) are significantly inhibited the free radical scavenging activity at higher doses, P
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    Free radical scavenging activity of broccoli extract (BE) and Trolox (a water soluble tocopherol analogue as a positive standard) against <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) (ABTS) radical formation. Negative controls were 0 μM BE or 0 μM Trolox. Results are means±SD of five measurements. Values with different letters (a–f) are significantly inhibited the free radical scavenging activity at higher doses, P
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    Free radical scavenging activity of broccoli extract (BE) and Trolox (a water soluble tocopherol analogue as a positive standard) against <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) (ABTS) radical formation. Negative controls were 0 μM BE or 0 μM Trolox. Results are means±SD of five measurements. Values with different letters (a–f) are significantly inhibited the free radical scavenging activity at higher doses, P
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    Free radical scavenging activity of broccoli extract (BE) and Trolox (a water soluble tocopherol analogue as a positive standard) against <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) (ABTS) radical formation. Negative controls were 0 μM BE or 0 μM Trolox. Results are means±SD of five measurements. Values with different letters (a–f) are significantly inhibited the free radical scavenging activity at higher doses, P
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    Free radical scavenging activity of broccoli extract (BE) and Trolox (a water soluble tocopherol analogue as a positive standard) against <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) (ABTS) radical formation. Negative controls were 0 μM BE or 0 μM Trolox. Results are means±SD of five measurements. Values with different letters (a–f) are significantly inhibited the free radical scavenging activity at higher doses, P
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    Free radical scavenging activity of broccoli extract (BE) and Trolox (a water soluble tocopherol analogue as a positive standard) against <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) (ABTS) radical formation. Negative controls were 0 μM BE or 0 μM Trolox. Results are means±SD of five measurements. Values with different letters (a–f) are significantly inhibited the free radical scavenging activity at higher doses, P
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    Avantor 2 2 azino bis
    Free radical scavenging activity of broccoli extract (BE) and Trolox (a water soluble tocopherol analogue as a positive standard) against <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) (ABTS) radical formation. Negative controls were 0 μM BE or 0 μM Trolox. Results are means±SD of five measurements. Values with different letters (a–f) are significantly inhibited the free radical scavenging activity at higher doses, P
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    BioVision 2 2 azino bis
    The in vitro experimental design and methodologies used to study the angiotensin I converting enzyme (ACE-I), renin, Platelet-activating factor-acetylhydrolase (PAF-AH) and dipeptidyl peptidase IV (DPP-IV) inhibition, and Total antioxidant capacity by <t>2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic</t> acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition based antioxidant activity of the gastric and small intestinal hydrolysates and the small intestinal
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    Nacalai 2 2 azino bis
    Relative effectiveness of different concentrations of the antioxidants in reducing 1,1′-diphenyl-2-picrylhydrazyl (DPPH) radicals ( A ) and the time course for the reaction of the antioxidants with <t>2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic</t> acid) (ABTS) radical cations ( B ). The activities are shown relative to fully reduced DPPH and ABTS (100%). The scavenger concentrations were 5 mg/mL. Ascorbic acid (VC) ( ), hyaluronic acid (HA) ( ) surface-deacetylated chitin nano-fiber (SDCH-NF) ( ), and untreated chitin (UCH) ( ).
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    Fluka Chemical 2 2 azino bis
    Relative effectiveness of different concentrations of the antioxidants in reducing 1,1′-diphenyl-2-picrylhydrazyl (DPPH) radicals ( A ) and the time course for the reaction of the antioxidants with <t>2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic</t> acid) (ABTS) radical cations ( B ). The activities are shown relative to fully reduced DPPH and ABTS (100%). The scavenger concentrations were 5 mg/mL. Ascorbic acid (VC) ( ), hyaluronic acid (HA) ( ) surface-deacetylated chitin nano-fiber (SDCH-NF) ( ), and untreated chitin (UCH) ( ).
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    Image Search Results


    Ecological and biochemical characteristics of the selected fungi. Note: ABTS = 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid; CMC = carboxymethyl cellulose.

    Journal: Microorganisms

    Article Title: Fungal Adaptation to the Advanced Stages of Wood Decomposition: Insights from the Steccherinum ochraceum

    doi: 10.3390/microorganisms7110527

    Figure Lengend Snippet: Ecological and biochemical characteristics of the selected fungi. Note: ABTS = 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid; CMC = carboxymethyl cellulose.

    Article Snippet: Express assays of oxidative enzymes were carried out in Petri dishes (Ø 90 mm) containing 20 mL of MEA and 0.1% w /v of 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS; Sigma, St. Louis, MO, USA).

    Techniques:

    Scatter plot of the PCA of the antioxidant capacity and metabolites determined in the plant material; ( A ) PC1 versus PC2, ( B ) PC1 versus PC3. Different colors describe groups of samples according to the cluster analysis ( Figure 3 ). TRB: callus from roots of plants with tomentose leaves growing on medium B, TLA: callus from leaves of plants with tomentose leaves growing on medium A, TLB: callus from leaves of plants with tomentose leaves growing on medium B, TLC: callus from leaves of plants with tomentose leaves growing on medium C, GLA: callus from leaves of plants with glabrous leaves growing on medium A, GLC: callus from leaves of plants with glabrous leaves growing on medium C, TRC: callus from roots of plants with tomentose leaves growing on medium C, GRB: callus from roots of plants with glabrous leaves growing on medium B, TLE: callus from leaves of plants with tomentose leaves growing on medium E, GRD: callus from roots of plants with glabrous leaves growing on medium D, GRE: callus from roots of plants with glabrous leaves growing on medium E, GLE: callus from leaves of plants with glabrous leaves growing on medium E, TRE: callus from roots of plants with tomentose leaves growing on medium E, TLD: callus from leaves of plants with tomentose leaves, growing on medium D, GRC: callus from roots of plants with glabrous leaves growing on medium C, GLD: callus from leaves of plants with glabrous leaves growing on medium D, SS: shoot of a plant grown in soil culture, RS: root of a plant grown in soil culture, SH: shoot of a plant grown in hydroponic culture, RH: root of a plant grown in hydroponic culture, UA: oleanolic acid, OA: ursolic acid, CO: carlina oxide, 3,5QA: 3,5-Di-caffeoylquinic acid, ChA: chlorogenic acid. FC: flavonoid content, DPPH, ABTS and TPC—antioxidant activity evaluated in tests with 2,2-diphenyl-1-picrylhydrazyl, 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid and Folin–Ciocalteu reagent, respectively.

    Journal: Molecules

    Article Title: The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of Carlina acaulis Plant Material

    doi: 10.3390/molecules25010146

    Figure Lengend Snippet: Scatter plot of the PCA of the antioxidant capacity and metabolites determined in the plant material; ( A ) PC1 versus PC2, ( B ) PC1 versus PC3. Different colors describe groups of samples according to the cluster analysis ( Figure 3 ). TRB: callus from roots of plants with tomentose leaves growing on medium B, TLA: callus from leaves of plants with tomentose leaves growing on medium A, TLB: callus from leaves of plants with tomentose leaves growing on medium B, TLC: callus from leaves of plants with tomentose leaves growing on medium C, GLA: callus from leaves of plants with glabrous leaves growing on medium A, GLC: callus from leaves of plants with glabrous leaves growing on medium C, TRC: callus from roots of plants with tomentose leaves growing on medium C, GRB: callus from roots of plants with glabrous leaves growing on medium B, TLE: callus from leaves of plants with tomentose leaves growing on medium E, GRD: callus from roots of plants with glabrous leaves growing on medium D, GRE: callus from roots of plants with glabrous leaves growing on medium E, GLE: callus from leaves of plants with glabrous leaves growing on medium E, TRE: callus from roots of plants with tomentose leaves growing on medium E, TLD: callus from leaves of plants with tomentose leaves, growing on medium D, GRC: callus from roots of plants with glabrous leaves growing on medium C, GLD: callus from leaves of plants with glabrous leaves growing on medium D, SS: shoot of a plant grown in soil culture, RS: root of a plant grown in soil culture, SH: shoot of a plant grown in hydroponic culture, RH: root of a plant grown in hydroponic culture, UA: oleanolic acid, OA: ursolic acid, CO: carlina oxide, 3,5QA: 3,5-Di-caffeoylquinic acid, ChA: chlorogenic acid. FC: flavonoid content, DPPH, ABTS and TPC—antioxidant activity evaluated in tests with 2,2-diphenyl-1-picrylhydrazyl, 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid and Folin–Ciocalteu reagent, respectively.

    Article Snippet: 2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Folin–Ciocalteu reagent, trolox, aluminum chloride, and standards of analytes: ChA (≥95.0%), 3,5CQA (≥95.0%), rutin (≥94.0%), UA (≥90.0%), and OA (≥97.0%) were purchased in Sigma (St. Louis, MO, USA).

    Techniques: Thin Layer Chromatography, Antioxidant Activity Assay

    The antioxidative activities of EGCG. ( a ) EGCG (0–25 μM) was reacted with 2,2-diphenyl-1-picrylhydrazyl (DPPH) in the dark at 37 °C for 30 min. Absorbance at 517 nm was measured by spectrophotometry; ( b ) 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and EGCG (0–25 μM) were incubated together at 37 °C for 30 min. Absorbance at 730 nm was measured by spectrophotometry. Ascorbic acid was used as a control compound; ( c ) RAW264.7 cells (1 × 10 6 cells/well) were treated with dihydrorhodamine 123 (DHR123) (20 μM) in the dark at 37 °C for 10 min. Cells were treated with EGCG (0–25 μM) for 20 min, and incubated additionally with sodium nitroprusside (SNP). The ROS level was determined by flowcytometric analysis; ( d ) HaCaT cells were pretreated with EGCG and then exposed to SNP for 24 h. SNP-derived NO production was analyzed by Griess assay; ( e ) the viability of SNP-treated cells was determined by an MTT assay; ( f ) HaCaT cells were incubated with EGCG and SNP for 24 h. Cleaved and total forms of caspases and β-actin expression were determined by immunoblotting. Statistical significance of results ( a – e ) was evaluated by Kruskal–Wallis/Mann–Whitney test. All data are expressed as means ± SD of an experiment. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Skin Protective Effect of Epigallocatechin Gallate

    doi: 10.3390/ijms19010173

    Figure Lengend Snippet: The antioxidative activities of EGCG. ( a ) EGCG (0–25 μM) was reacted with 2,2-diphenyl-1-picrylhydrazyl (DPPH) in the dark at 37 °C for 30 min. Absorbance at 517 nm was measured by spectrophotometry; ( b ) 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and EGCG (0–25 μM) were incubated together at 37 °C for 30 min. Absorbance at 730 nm was measured by spectrophotometry. Ascorbic acid was used as a control compound; ( c ) RAW264.7 cells (1 × 10 6 cells/well) were treated with dihydrorhodamine 123 (DHR123) (20 μM) in the dark at 37 °C for 10 min. Cells were treated with EGCG (0–25 μM) for 20 min, and incubated additionally with sodium nitroprusside (SNP). The ROS level was determined by flowcytometric analysis; ( d ) HaCaT cells were pretreated with EGCG and then exposed to SNP for 24 h. SNP-derived NO production was analyzed by Griess assay; ( e ) the viability of SNP-treated cells was determined by an MTT assay; ( f ) HaCaT cells were incubated with EGCG and SNP for 24 h. Cleaved and total forms of caspases and β-actin expression were determined by immunoblotting. Statistical significance of results ( a – e ) was evaluated by Kruskal–Wallis/Mann–Whitney test. All data are expressed as means ± SD of an experiment. * p

    Article Snippet: Polyethylenimine (PEI), 1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), ascorbic acid, sodium nitroprusside (SNP), dehydrorhodamine 123 (DHR123), α-melanocyte stimulating hormone (αMSH), TRIzol, and arbutin were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO, USA).

    Techniques: Spectrophotometry, Incubation, Derivative Assay, Griess Assay, MTT Assay, Expressing, MANN-WHITNEY

    The correlations between total phenolic content and antioxidant activity from: ( A ) ferric reducing antioxidant power (FRAP) assay; ( B ) 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay; ( C ) 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay; and ( D ) ferric thiocyanate method. Data are the mean value ± S.D. of three independent experiments.

    Journal: Nutrients

    Article Title: Inhibition of 5α-Reductase, IL-6 Secretion, and Oxidation Process of Equisetum debile Roxb. ex Vaucher Extract as Functional Food and Nutraceuticals Ingredients

    doi: 10.3390/nu9101105

    Figure Lengend Snippet: The correlations between total phenolic content and antioxidant activity from: ( A ) ferric reducing antioxidant power (FRAP) assay; ( B ) 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay; ( C ) 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay; and ( D ) ferric thiocyanate method. Data are the mean value ± S.D. of three independent experiments.

    Article Snippet: Chemical Materials Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt (NADPH), Folin–Ciocalteu reagent, 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,4,6 tripyridyl-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), gallic acid, 2,2′-diphenyl-1-picrylhydrazyl (DPPH), lipopolysaccharide (LPS), linoleic acid, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) and 3-(4,5-dimethylthiazolyl-2) -2,5-diphenyl tetrazolium bromide (MTT), were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Antioxidant Activity Assay, FRAP Assay, DPPH Assay, ABTS Assay

    The effects of the concentration of ( a ) whey protein isolate (WPI) hydrolysate, ( b ) the mass fraction of NaH 2 PO 4 , ( c ) 1-propanol and ( d ) NaCl on the separation of antioxidant peptides. The scavenging was 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging activity per unit concentration in top phase of Aqueous two-phase extraction (ATPE).

    Journal: Foods

    Article Title: Separation and Enrichment of Antioxidant Peptides from Whey Protein Isolate Hydrolysate by Aqueous Two-Phase Extraction and Aqueous Two-Phase Flotation

    doi: 10.3390/foods8010034

    Figure Lengend Snippet: The effects of the concentration of ( a ) whey protein isolate (WPI) hydrolysate, ( b ) the mass fraction of NaH 2 PO 4 , ( c ) 1-propanol and ( d ) NaCl on the separation of antioxidant peptides. The scavenging was 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging activity per unit concentration in top phase of Aqueous two-phase extraction (ATPE).

    Article Snippet: 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (Sigma, USA) and 2,2-diphenyl-1-picrylhydrazyl (Sigma, USA) were utilized to analyze the bioactive peptides.

    Techniques: Concentration Assay, Activity Assay

    CsUGT78A14 confer flavonoids enhanced reactive oxygen species (ROS) scavenging capacity. ROS scavenging activity of reaction products formed by CsUGT78A14-1 and control was compared. Kaempferol (A , C , E) and quercetin (B , D , F) were used as substrates. ROS scavenger capacity of kaempferol and quercetin incubated with CsUGT78A14-1 was significantly enhanced compared to the control by 2,2-diphenyl-1-picrylhydrazyl (A , B) , ferric reducing ability of plasma (C , D) , and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) method (E , F) . Three experimental replicates were conducted for the ROS scavenging capacity analysis. NS, P=0.05141.

    Journal: Frontiers in Plant Science

    Article Title: Glucosyltransferase CsUGT78A14 Regulates Flavonols Accumulation and Reactive Oxygen Species Scavenging in Response to Cold Stress in Camellia sinensis

    doi: 10.3389/fpls.2019.01675

    Figure Lengend Snippet: CsUGT78A14 confer flavonoids enhanced reactive oxygen species (ROS) scavenging capacity. ROS scavenging activity of reaction products formed by CsUGT78A14-1 and control was compared. Kaempferol (A , C , E) and quercetin (B , D , F) were used as substrates. ROS scavenger capacity of kaempferol and quercetin incubated with CsUGT78A14-1 was significantly enhanced compared to the control by 2,2-diphenyl-1-picrylhydrazyl (A , B) , ferric reducing ability of plasma (C , D) , and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) method (E , F) . Three experimental replicates were conducted for the ROS scavenging capacity analysis. NS, P=0.05141.

    Article Snippet: Determination of Antioxidant Activity and Reactive Oxygen Species Content The total antioxidant activity was determined using the ferric reducing ability of plasma (FRAP) method with a FRAP reagent kit (Beyotime, Shanghai, China), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method (Beyotime, Shanghai, China), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) as described previously ( ).

    Techniques: Activity Assay, Incubation

    Effects of methods of burdock root tea (BRT) preparation on antioxidant activities. In order to elucidate the effects of BRT preparation conditions on antioxidant activity, roasting number and tea extraction time were varied. Burdock roots were roasted 0, 3, or 6 times, and BRT was produced by boiling these roasted burdock roots (1.00 mg/mL) for 30 or 60 min at 100°C. Total antioxidant capacities of BRT were determined based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (A), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Results are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–e) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.

    Journal: Preventive Nutrition and Food Science

    Article Title: Influence of Roasting Treatment on the Antioxidant Activities and Color of Burdock Root Tea

    doi: 10.3746/pnf.2017.22.1.21

    Figure Lengend Snippet: Effects of methods of burdock root tea (BRT) preparation on antioxidant activities. In order to elucidate the effects of BRT preparation conditions on antioxidant activity, roasting number and tea extraction time were varied. Burdock roots were roasted 0, 3, or 6 times, and BRT was produced by boiling these roasted burdock roots (1.00 mg/mL) for 30 or 60 min at 100°C. Total antioxidant capacities of BRT were determined based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (A), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Results are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–e) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.

    Article Snippet: Pyrocatechol violet (PV) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

    Techniques: Antioxidant Activity Assay, Produced, Activity Assay, Standard Deviation

    Comparison of antioxidant activities of burdock root tea (BRT) and burdock methanol extract. BRT was produced by boiling burdock roots (1.00 mg/mL) roasted six times in water at 100°C for 60 min. Methanol extract of burdock roots was prepared by extracting burdock roots (1.00 mg/mL) roasted six times in methanol at 30°C for 24 h. Burdock root extracts were subjected to total antioxidant capacity assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (A), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Values are expressed as the mean±standard deviation (n=6). *** P

    Journal: Preventive Nutrition and Food Science

    Article Title: Influence of Roasting Treatment on the Antioxidant Activities and Color of Burdock Root Tea

    doi: 10.3746/pnf.2017.22.1.21

    Figure Lengend Snippet: Comparison of antioxidant activities of burdock root tea (BRT) and burdock methanol extract. BRT was produced by boiling burdock roots (1.00 mg/mL) roasted six times in water at 100°C for 60 min. Methanol extract of burdock roots was prepared by extracting burdock roots (1.00 mg/mL) roasted six times in methanol at 30°C for 24 h. Burdock root extracts were subjected to total antioxidant capacity assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (A), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Values are expressed as the mean±standard deviation (n=6). *** P

    Article Snippet: Pyrocatechol violet (PV) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

    Techniques: Produced, Activity Assay, Standard Deviation

    Effects of amount of burdock root tea (BRT) on antioxidant activities. In order to examine the effect of concentration, BRT was prepared by boiling burdock roots roasted six times at various concentrations (0.10, 0.25, 0.50, and 1.00 mg/mL) for 60 min. Total antioxidant capacities of BRT were tested based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (A), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Data are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–d) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.

    Journal: Preventive Nutrition and Food Science

    Article Title: Influence of Roasting Treatment on the Antioxidant Activities and Color of Burdock Root Tea

    doi: 10.3746/pnf.2017.22.1.21

    Figure Lengend Snippet: Effects of amount of burdock root tea (BRT) on antioxidant activities. In order to examine the effect of concentration, BRT was prepared by boiling burdock roots roasted six times at various concentrations (0.10, 0.25, 0.50, and 1.00 mg/mL) for 60 min. Total antioxidant capacities of BRT were tested based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (A), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (B), ferric reducing capacity (C), copper chelating activity (D), total polyphenol contents (E), and flavonoid contents (F). Data are expressed as the mean±standard deviation (n=6). Mean sharing the same letter (a–d) are not significantly different at the 5% level. AA, ascorbic acid equivalent; TE, Trolox equivalents; GAE, gallic acid equivalents; CE, catechin equivalents.

    Article Snippet: Pyrocatechol violet (PV) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

    Techniques: Concentration Assay, Activity Assay, Standard Deviation

    Characterization of the aptamers recognizing the Leishmania and human PABP1 proteins . ( a ) Recombinant GST-PABP protein or GST were plated at 500 ng/well and incubated with the digoxigenin-labeled aptamers (10 nmol/l). Anti-digoxigenin-POD antibodies were added for 1 hour and revealed with an 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) solution at 405 nm. All the experiments were performed in triplicate and the average of three different experiments is shown: * P

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Inhibition of Influenza Virus Replication by DNA Aptamers Targeting a Cellular Component of Translation Initiation

    doi: 10.1038/mtna.2016.20

    Figure Lengend Snippet: Characterization of the aptamers recognizing the Leishmania and human PABP1 proteins . ( a ) Recombinant GST-PABP protein or GST were plated at 500 ng/well and incubated with the digoxigenin-labeled aptamers (10 nmol/l). Anti-digoxigenin-POD antibodies were added for 1 hour and revealed with an 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) solution at 405 nm. All the experiments were performed in triplicate and the average of three different experiments is shown: * P

    Article Snippet: The plates were then washed four times and antibody binding was visualized with an 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) solution (Boehringer–Mannheim, Mannheim, Germany) according to the manufacturer's instruction.

    Techniques: Recombinant, Incubation, Labeling

    Free radical scavenging activity of broccoli extract (BE) and Trolox (a water soluble tocopherol analogue as a positive standard) against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical formation. Negative controls were 0 μM BE or 0 μM Trolox. Results are means±SD of five measurements. Values with different letters (a–f) are significantly inhibited the free radical scavenging activity at higher doses, P

    Journal: Preventive Nutrition and Food Science

    Article Title: Broccoli (Brassica oleracea) Reduces Oxidative Damage to Pancreatic Tissue and Combats Hyperglycaemia in Diabetic Rats

    doi: 10.3746/pnf.2017.22.4.277

    Figure Lengend Snippet: Free radical scavenging activity of broccoli extract (BE) and Trolox (a water soluble tocopherol analogue as a positive standard) against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical formation. Negative controls were 0 μM BE or 0 μM Trolox. Results are means±SD of five measurements. Values with different letters (a–f) are significantly inhibited the free radical scavenging activity at higher doses, P

    Article Snippet: Evaluation of the antioxidant activity of BE A colorimetric method using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) Antioxidant Assay Kit (Cat#AOX-1, Zen-Bio, Durham, NC, USA) was used.

    Techniques: Activity Assay

    The in vitro experimental design and methodologies used to study the angiotensin I converting enzyme (ACE-I), renin, Platelet-activating factor-acetylhydrolase (PAF-AH) and dipeptidyl peptidase IV (DPP-IV) inhibition, and Total antioxidant capacity by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition based antioxidant activity of the gastric and small intestinal hydrolysates and the small intestinal

    Journal: International Journal of Molecular Sciences

    Article Title: Gastrointestinal Endogenous Protein-Derived Bioactive Peptides: An in Vitro Study of Their Gut Modulatory Potential

    doi: 10.3390/ijms17040482

    Figure Lengend Snippet: The in vitro experimental design and methodologies used to study the angiotensin I converting enzyme (ACE-I), renin, Platelet-activating factor-acetylhydrolase (PAF-AH) and dipeptidyl peptidase IV (DPP-IV) inhibition, and Total antioxidant capacity by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition based antioxidant activity of the gastric and small intestinal hydrolysates and the small intestinal

    Article Snippet: The ACE-I inhibition assay kit was supplied by Dojindo EU, GmbH (Kumamoto, Japan), the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) inhibition based total antioxidant capacity assay kit was supplied by BioVision Inc. (Milpitas, CA, USA), the ABTS total antioxidant capacity assay kit was supplied by Zen-Bio, Inc. (Research Triangle Park, NC, USA) and the renin, PAF-AH and DPP-IV inhibition assay kits were all supplied by the Cayman Chemical Company (Cambridge BioSciences, Cambridge, UK).

    Techniques: In Vitro, Inhibition, Antioxidant Activity Assay

    Total antioxidant capacity by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) inhibition by ( A ) gastric (G) and small intestinal digests (G + SI) of the gastrointestinal endogenous proteins, porcine trypsin (TRYP), human lysozyme (LYS), porcine salivary mucin (MUC) and human serum albumin (SA), and dietary protein chicken albumin (CA); and ( B )

    Journal: International Journal of Molecular Sciences

    Article Title: Gastrointestinal Endogenous Protein-Derived Bioactive Peptides: An in Vitro Study of Their Gut Modulatory Potential

    doi: 10.3390/ijms17040482

    Figure Lengend Snippet: Total antioxidant capacity by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) inhibition by ( A ) gastric (G) and small intestinal digests (G + SI) of the gastrointestinal endogenous proteins, porcine trypsin (TRYP), human lysozyme (LYS), porcine salivary mucin (MUC) and human serum albumin (SA), and dietary protein chicken albumin (CA); and ( B )

    Article Snippet: The ACE-I inhibition assay kit was supplied by Dojindo EU, GmbH (Kumamoto, Japan), the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) inhibition based total antioxidant capacity assay kit was supplied by BioVision Inc. (Milpitas, CA, USA), the ABTS total antioxidant capacity assay kit was supplied by Zen-Bio, Inc. (Research Triangle Park, NC, USA) and the renin, PAF-AH and DPP-IV inhibition assay kits were all supplied by the Cayman Chemical Company (Cambridge BioSciences, Cambridge, UK).

    Techniques: Inhibition

    Relative effectiveness of different concentrations of the antioxidants in reducing 1,1′-diphenyl-2-picrylhydrazyl (DPPH) radicals ( A ) and the time course for the reaction of the antioxidants with 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cations ( B ). The activities are shown relative to fully reduced DPPH and ABTS (100%). The scavenger concentrations were 5 mg/mL. Ascorbic acid (VC) ( ), hyaluronic acid (HA) ( ) surface-deacetylated chitin nano-fiber (SDCH-NF) ( ), and untreated chitin (UCH) ( ).

    Journal: International Journal of Molecular Sciences

    Article Title: Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets

    doi: 10.3390/ijms161024707

    Figure Lengend Snippet: Relative effectiveness of different concentrations of the antioxidants in reducing 1,1′-diphenyl-2-picrylhydrazyl (DPPH) radicals ( A ) and the time course for the reaction of the antioxidants with 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cations ( B ). The activities are shown relative to fully reduced DPPH and ABTS (100%). The scavenger concentrations were 5 mg/mL. Ascorbic acid (VC) ( ), hyaluronic acid (HA) ( ) surface-deacetylated chitin nano-fiber (SDCH-NF) ( ), and untreated chitin (UCH) ( ).

    Article Snippet: 1,1′-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were supplied by Nacalai Tesque (Kyoto, Japan).

    Techniques: