183 Search Results


97
Danaher Inc flexstation 3
Flexstation 3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
ATCC nci h661
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Nci H661, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
ATCC d 17 ccl 183 cell line
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D 17 Ccl 183 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Developmental Studies Hybridoma Bank mouse monoclonal anti proteasomal subunit ps
Loss of cln5 increases the abundance of a <t>proteasomal</t> subunit during growth and starvation but decreases proteasome 20S activity. (A) Whole cell (WC) lysates from growth-phase and 4 h starved WT and cln5 - - cells were separated by SDS-PAGE and analyzed by western blotting. Membranes were probed with anti-PS, anti-PS5, and anti-β-actin (loading control). Fiji/ImageJ was used to quantify (B) PS5 and (C) PS protein bands, which were then standardized against the levels of β-actin. Data presented as mean protein amount (% WT) ± SEM ( n ≥ 11). * p < 0.05 (one sample t -test). NS – not significant. (D) Proteasome 20S activity was assessed by collecting WT and cln5 - cells during growth and after 4 h of starvation. Cells were then lysed and proteasome 20S activity was measured using a commercially available kit. Raw activity values were subtracted from the blank solution and activities in cln5 -deficient lysates were standardized against the activities in WT samples. Data presented as mean proteasome 20S activity (% WT) ± SEM (n = 11). * p < 0.05 (one sample t -test).
Mouse Monoclonal Anti Proteasomal Subunit Ps, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
ATCC serratia plymuthica
Loss of cln5 increases the abundance of a <t>proteasomal</t> subunit during growth and starvation but decreases proteasome 20S activity. (A) Whole cell (WC) lysates from growth-phase and 4 h starved WT and cln5 - - cells were separated by SDS-PAGE and analyzed by western blotting. Membranes were probed with anti-PS, anti-PS5, and anti-β-actin (loading control). Fiji/ImageJ was used to quantify (B) PS5 and (C) PS protein bands, which were then standardized against the levels of β-actin. Data presented as mean protein amount (% WT) ± SEM ( n ≥ 11). * p < 0.05 (one sample t -test). NS – not significant. (D) Proteasome 20S activity was assessed by collecting WT and cln5 - cells during growth and after 4 h of starvation. Cells were then lysed and proteasome 20S activity was measured using a commercially available kit. Raw activity values were subtracted from the blank solution and activities in cln5 -deficient lysates were standardized against the activities in WT samples. Data presented as mean proteasome 20S activity (% WT) ± SEM (n = 11). * p < 0.05 (one sample t -test).
Serratia Plymuthica, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


KEY RESOURCES TABLE

Journal: Cancer cell

Article Title: Binary pan-cancer classes with distinct vulnerabilities defined by pro- or anti-cancer YAP/TEAD activity

doi: 10.1016/j.ccell.2021.06.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: NCI-H661 , ATCC , Cat# HTB-183; RRID: CVCL_1577.

Techniques: Plasmid Preparation, Derivative Assay, Recombinant, Electron Microscopy, Staining, SYBR Green Assay, Sample Prep, Microarray, Expressing, CRISPR, Software, Flow Cytometry

Loss of cln5 increases the abundance of a proteasomal subunit during growth and starvation but decreases proteasome 20S activity. (A) Whole cell (WC) lysates from growth-phase and 4 h starved WT and cln5 - - cells were separated by SDS-PAGE and analyzed by western blotting. Membranes were probed with anti-PS, anti-PS5, and anti-β-actin (loading control). Fiji/ImageJ was used to quantify (B) PS5 and (C) PS protein bands, which were then standardized against the levels of β-actin. Data presented as mean protein amount (% WT) ± SEM ( n ≥ 11). * p < 0.05 (one sample t -test). NS – not significant. (D) Proteasome 20S activity was assessed by collecting WT and cln5 - cells during growth and after 4 h of starvation. Cells were then lysed and proteasome 20S activity was measured using a commercially available kit. Raw activity values were subtracted from the blank solution and activities in cln5 -deficient lysates were standardized against the activities in WT samples. Data presented as mean proteasome 20S activity (% WT) ± SEM (n = 11). * p < 0.05 (one sample t -test).

Journal: Frontiers in Genetics

Article Title: An altered transcriptome underlies cln5 -deficiency phenotypes in Dictyostelium discoideum

doi: 10.3389/fgene.2022.1045738

Figure Lengend Snippet: Loss of cln5 increases the abundance of a proteasomal subunit during growth and starvation but decreases proteasome 20S activity. (A) Whole cell (WC) lysates from growth-phase and 4 h starved WT and cln5 - - cells were separated by SDS-PAGE and analyzed by western blotting. Membranes were probed with anti-PS, anti-PS5, and anti-β-actin (loading control). Fiji/ImageJ was used to quantify (B) PS5 and (C) PS protein bands, which were then standardized against the levels of β-actin. Data presented as mean protein amount (% WT) ± SEM ( n ≥ 11). * p < 0.05 (one sample t -test). NS – not significant. (D) Proteasome 20S activity was assessed by collecting WT and cln5 - cells during growth and after 4 h of starvation. Cells were then lysed and proteasome 20S activity was measured using a commercially available kit. Raw activity values were subtracted from the blank solution and activities in cln5 -deficient lysates were standardized against the activities in WT samples. Data presented as mean proteasome 20S activity (% WT) ± SEM (n = 11). * p < 0.05 (one sample t -test).

Article Snippet: Mouse monoclonal anti-cortexillin-I (CtxA) (241-438-1) , mouse monoclonal anti-cortexillin-II (CtxB) (232-238-10) , mouse monoclonal anti-discoidin (Dsc) (80-52-13) , mouse monoclonal myosin heavy chain type II (MhcA) (56-396-5) , mouse monoclonal anti-proteasomal subunit (PS) (159-183-10) , and mouse monoclonal anti-proteasomal subunit 5 (PS5) (171-337) ( ) were purchased from the Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, Iowa, United States).

Techniques: Activity Assay, SDS Page, Western Blot