18094 Search Results


clock  (Proteintech)
94
Proteintech clock
Res cells enter quiescence with prolonged circadian period. After cells (UMUC3 and EJ, Res and Res + PTX) were synchronized with 0.1 µM dexamethasone for 2 h, circadian genes were detected at different time points. (A) mRNA expression levels of circadian <t>clock</t> genes (BMAL1, CLOCK, PER2 <t>and</t> <t>CRY1)</t> and corresponding fitted cosinor curves. (B) Time course of protein (BMAL1, CLOCK, PER2 and CRY1) expression levels in the Res and Res + PTX (80 nM) cells. β-Actin was used as loading control. Data are presented as the OD fold difference related to the control from three duplicate experiments. Res, cisplatin-resistant cells; PTX, paclitaxel; OD, optical density; ZT, circadian time; CRY1, Cryptochrome 1; PER2, period 2; CLOCK, circadian locomotor output cycles kaput; BMAL1, brain and muscle Arnt-like protein 1.
Clock, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clock/product/Proteintech
Average 94 stars, based on 1 article reviews
clock - by Bioz Stars, 2026-02
94/100 stars
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sphingomonas kaistensis kacc 14226t  (DSMZ)
86
DSMZ sphingomonas kaistensis kacc 14226t
Res cells enter quiescence with prolonged circadian period. After cells (UMUC3 and EJ, Res and Res + PTX) were synchronized with 0.1 µM dexamethasone for 2 h, circadian genes were detected at different time points. (A) mRNA expression levels of circadian <t>clock</t> genes (BMAL1, CLOCK, PER2 <t>and</t> <t>CRY1)</t> and corresponding fitted cosinor curves. (B) Time course of protein (BMAL1, CLOCK, PER2 and CRY1) expression levels in the Res and Res + PTX (80 nM) cells. β-Actin was used as loading control. Data are presented as the OD fold difference related to the control from three duplicate experiments. Res, cisplatin-resistant cells; PTX, paclitaxel; OD, optical density; ZT, circadian time; CRY1, Cryptochrome 1; PER2, period 2; CLOCK, circadian locomotor output cycles kaput; BMAL1, brain and muscle Arnt-like protein 1.
Sphingomonas Kaistensis Kacc 14226t, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphingomonas kaistensis kacc 14226t/product/DSMZ
Average 86 stars, based on 1 article reviews
sphingomonas kaistensis kacc 14226t - by Bioz Stars, 2026-02
86/100 stars
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Image Search Results


Res cells enter quiescence with prolonged circadian period. After cells (UMUC3 and EJ, Res and Res + PTX) were synchronized with 0.1 µM dexamethasone for 2 h, circadian genes were detected at different time points. (A) mRNA expression levels of circadian clock genes (BMAL1, CLOCK, PER2 and CRY1) and corresponding fitted cosinor curves. (B) Time course of protein (BMAL1, CLOCK, PER2 and CRY1) expression levels in the Res and Res + PTX (80 nM) cells. β-Actin was used as loading control. Data are presented as the OD fold difference related to the control from three duplicate experiments. Res, cisplatin-resistant cells; PTX, paclitaxel; OD, optical density; ZT, circadian time; CRY1, Cryptochrome 1; PER2, period 2; CLOCK, circadian locomotor output cycles kaput; BMAL1, brain and muscle Arnt-like protein 1.

Journal: Oncology Reports

Article Title: Circadian clock protein CRY1 prevents paclitaxel-induced senescence of bladder cancer cells by promoting p53 degradation

doi: 10.3892/or.2020.7914

Figure Lengend Snippet: Res cells enter quiescence with prolonged circadian period. After cells (UMUC3 and EJ, Res and Res + PTX) were synchronized with 0.1 µM dexamethasone for 2 h, circadian genes were detected at different time points. (A) mRNA expression levels of circadian clock genes (BMAL1, CLOCK, PER2 and CRY1) and corresponding fitted cosinor curves. (B) Time course of protein (BMAL1, CLOCK, PER2 and CRY1) expression levels in the Res and Res + PTX (80 nM) cells. β-Actin was used as loading control. Data are presented as the OD fold difference related to the control from three duplicate experiments. Res, cisplatin-resistant cells; PTX, paclitaxel; OD, optical density; ZT, circadian time; CRY1, Cryptochrome 1; PER2, period 2; CLOCK, circadian locomotor output cycles kaput; BMAL1, brain and muscle Arnt-like protein 1.

Article Snippet: The primary antibodies are as follows: CRY1 (cat. no. ab245564; Abcam), PER2 (cat. no. ab179813; Abcam), CLOCK (cat. no. 18094-1-AP; ProteinTech Group, Inc.), BMAL1 (cat. no. 14268-1-AP; ProteinTech Group, Inc.), p53 (cat. no. 2524; Cell Signaling Technology, Inc.), p21 (cat. no. 10355-1-AP; ProteinTech Group, Inc.) and GAPDH (cat. no. AP0063; Biogot Technology Co., Ltd.).

Techniques: Expressing, Control

Expression levels of circadian clock proteins in the Res cells with PTX treatment. (A) Total extracts of cells were harvested at 2 h post-dexamethasone (0.1 µM). The expression levels of β-Actin and circadian clock proteins, including BMAL1, CLOCK, PER2 and CRY1, were assessed via western blot analysis. (B) EJ and UMUC3 cells were treated with serum-free medium for 24, 48 and 72 h. The expression levels of BMAL1, CLOCK, PER2 and CRY1 were detected using western blotting. Data are presented as the OD fold difference related to the control from three duplicate experiments. *P<0.05. CRY1, Cryptochrome 1; PER2, period 2; CLOCK, circadian locomotor output cycles kaput; BMAL1, brain and muscle Arnt-like protein 1; Res, cisplatin-resistant cells; PTX, paclitaxel; OD, optical density.

Journal: Oncology Reports

Article Title: Circadian clock protein CRY1 prevents paclitaxel-induced senescence of bladder cancer cells by promoting p53 degradation

doi: 10.3892/or.2020.7914

Figure Lengend Snippet: Expression levels of circadian clock proteins in the Res cells with PTX treatment. (A) Total extracts of cells were harvested at 2 h post-dexamethasone (0.1 µM). The expression levels of β-Actin and circadian clock proteins, including BMAL1, CLOCK, PER2 and CRY1, were assessed via western blot analysis. (B) EJ and UMUC3 cells were treated with serum-free medium for 24, 48 and 72 h. The expression levels of BMAL1, CLOCK, PER2 and CRY1 were detected using western blotting. Data are presented as the OD fold difference related to the control from three duplicate experiments. *P<0.05. CRY1, Cryptochrome 1; PER2, period 2; CLOCK, circadian locomotor output cycles kaput; BMAL1, brain and muscle Arnt-like protein 1; Res, cisplatin-resistant cells; PTX, paclitaxel; OD, optical density.

Article Snippet: The primary antibodies are as follows: CRY1 (cat. no. ab245564; Abcam), PER2 (cat. no. ab179813; Abcam), CLOCK (cat. no. 18094-1-AP; ProteinTech Group, Inc.), BMAL1 (cat. no. 14268-1-AP; ProteinTech Group, Inc.), p53 (cat. no. 2524; Cell Signaling Technology, Inc.), p21 (cat. no. 10355-1-AP; ProteinTech Group, Inc.) and GAPDH (cat. no. AP0063; Biogot Technology Co., Ltd.).

Techniques: Expressing, Western Blot, Control