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  • 89
    MedChemExpress salmeterol xinafoate
    Salmeterol Xinafoate, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC p putida atcc 17453
    Dedicated pathway for the degradation of (+)- and (-)-camphor (1) by Pseudomonas putida ATCC 17453. Key idiosyncratic pathway intermediates shown are the enantiomeric diketocamphanes (2, DKCs), the monocyclic ketone 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA (3, HTP-CoA) itself formed from the corresponding transitory free acid precursor OTE (2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetic acid), and the unstable monocyclic lactone 5-hydroxy-3,4,4-trimethyl-△ 2 -pimelyl- δ -CoA lactone (4). Subsequent hydrolysis of the monocyclic lactone generates the first aliphatic intermediate, a C10 carboxylic acid that is further metabolised to the TCA cycle intermediates acetyl-CoA and succinyl-CoA. The key sequential enzyme-catalysed steps are: a. camphor 5-monooxygenase ( camCAB ); b. exo hydroxycamphor dehydrogenase ( camD ); c. 2,5-diketocamphane 1,2-monooxygenase ( camE 25-1 + camE 25-2 ); d. 3,6-diketocamphane 1,6-monooxygenase ( camE 36 ); e. 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase ( camG ).
    P Putida Atcc 17453, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Addgene inc pentr pter
    Dedicated pathway for the degradation of (+)- and (-)-camphor (1) by Pseudomonas putida ATCC 17453. Key idiosyncratic pathway intermediates shown are the enantiomeric diketocamphanes (2, DKCs), the monocyclic ketone 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA (3, HTP-CoA) itself formed from the corresponding transitory free acid precursor OTE (2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetic acid), and the unstable monocyclic lactone 5-hydroxy-3,4,4-trimethyl-△ 2 -pimelyl- δ -CoA lactone (4). Subsequent hydrolysis of the monocyclic lactone generates the first aliphatic intermediate, a C10 carboxylic acid that is further metabolised to the TCA cycle intermediates acetyl-CoA and succinyl-CoA. The key sequential enzyme-catalysed steps are: a. camphor 5-monooxygenase ( camCAB ); b. exo hydroxycamphor dehydrogenase ( camD ); c. 2,5-diketocamphane 1,2-monooxygenase ( camE 25-1 + camE 25-2 ); d. 3,6-diketocamphane 1,6-monooxygenase ( camE 36 ); e. 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase ( camG ).
    Pentr Pter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Proteintech anti coq5
    Dedicated pathway for the degradation of (+)- and (-)-camphor (1) by Pseudomonas putida ATCC 17453. Key idiosyncratic pathway intermediates shown are the enantiomeric diketocamphanes (2, DKCs), the monocyclic ketone 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA (3, HTP-CoA) itself formed from the corresponding transitory free acid precursor OTE (2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetic acid), and the unstable monocyclic lactone 5-hydroxy-3,4,4-trimethyl-△ 2 -pimelyl- δ -CoA lactone (4). Subsequent hydrolysis of the monocyclic lactone generates the first aliphatic intermediate, a C10 carboxylic acid that is further metabolised to the TCA cycle intermediates acetyl-CoA and succinyl-CoA. The key sequential enzyme-catalysed steps are: a. camphor 5-monooxygenase ( camCAB ); b. exo hydroxycamphor dehydrogenase ( camD ); c. 2,5-diketocamphane 1,2-monooxygenase ( camE 25-1 + camE 25-2 ); d. 3,6-diketocamphane 1,6-monooxygenase ( camE 36 ); e. 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase ( camG ).
    Anti Coq5, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore 17453 8d 5 source novagen novagen novagen
    Dedicated pathway for the degradation of (+)- and (-)-camphor (1) by Pseudomonas putida ATCC 17453. Key idiosyncratic pathway intermediates shown are the enantiomeric diketocamphanes (2, DKCs), the monocyclic ketone 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA (3, HTP-CoA) itself formed from the corresponding transitory free acid precursor OTE (2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetic acid), and the unstable monocyclic lactone 5-hydroxy-3,4,4-trimethyl-△ 2 -pimelyl- δ -CoA lactone (4). Subsequent hydrolysis of the monocyclic lactone generates the first aliphatic intermediate, a C10 carboxylic acid that is further metabolised to the TCA cycle intermediates acetyl-CoA and succinyl-CoA. The key sequential enzyme-catalysed steps are: a. camphor 5-monooxygenase ( camCAB ); b. exo hydroxycamphor dehydrogenase ( camD ); c. 2,5-diketocamphane 1,2-monooxygenase ( camE 25-1 + camE 25-2 ); d. 3,6-diketocamphane 1,6-monooxygenase ( camE 36 ); e. 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase ( camG ).
    17453 8d 5 Source Novagen Novagen Novagen, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Dedicated pathway for the degradation of (+)- and (-)-camphor (1) by Pseudomonas putida ATCC 17453. Key idiosyncratic pathway intermediates shown are the enantiomeric diketocamphanes (2, DKCs), the monocyclic ketone 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA (3, HTP-CoA) itself formed from the corresponding transitory free acid precursor OTE (2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetic acid), and the unstable monocyclic lactone 5-hydroxy-3,4,4-trimethyl-△ 2 -pimelyl- δ -CoA lactone (4). Subsequent hydrolysis of the monocyclic lactone generates the first aliphatic intermediate, a C10 carboxylic acid that is further metabolised to the TCA cycle intermediates acetyl-CoA and succinyl-CoA. The key sequential enzyme-catalysed steps are: a. camphor 5-monooxygenase ( camCAB ); b. exo hydroxycamphor dehydrogenase ( camD ); c. 2,5-diketocamphane 1,2-monooxygenase ( camE 25-1 + camE 25-2 ); d. 3,6-diketocamphane 1,6-monooxygenase ( camE 36 ); e. 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase ( camG ).

    Journal: Microorganisms

    Article Title: The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453—An Episodic History and Still Mysterious after 60 Years

    doi: 10.3390/microorganisms9122593

    Figure Lengend Snippet: Dedicated pathway for the degradation of (+)- and (-)-camphor (1) by Pseudomonas putida ATCC 17453. Key idiosyncratic pathway intermediates shown are the enantiomeric diketocamphanes (2, DKCs), the monocyclic ketone 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA (3, HTP-CoA) itself formed from the corresponding transitory free acid precursor OTE (2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetic acid), and the unstable monocyclic lactone 5-hydroxy-3,4,4-trimethyl-△ 2 -pimelyl- δ -CoA lactone (4). Subsequent hydrolysis of the monocyclic lactone generates the first aliphatic intermediate, a C10 carboxylic acid that is further metabolised to the TCA cycle intermediates acetyl-CoA and succinyl-CoA. The key sequential enzyme-catalysed steps are: a. camphor 5-monooxygenase ( camCAB ); b. exo hydroxycamphor dehydrogenase ( camD ); c. 2,5-diketocamphane 1,2-monooxygenase ( camE 25-1 + camE 25-2 ); d. 3,6-diketocamphane 1,6-monooxygenase ( camE 36 ); e. 2-oxo-△ 3,4 -4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase ( camG ).

    Article Snippet: Now, over 60 years later, it has progressed sufficiently to validate another of Gunsalus’s astute predictions by confirming that the CAM plasmid of P putida ATCC 17453 can function as a totally autonomous metabolic entity.

    Techniques:

    Localization of additional genes and predicted open reading frames (ORFs) flanking the established initial genes of the camphor camDCAB operon and its repressor, camR , on an~40.5-kb established sequenced region of the CAM plasmid of P. putida ATCC 17453. The predicted ORFs or genes are sequenced numbered from 1 to 27, except for the established camRDCAB genes, which are shaded in black. The numbered from 1 to 27, except for the established camRDCAB genes, which are shaded in black. The orientation of the arrows indicates the direction of gene transcription. The candidate genes of this study ( camE 25–1 , camE 25–2 , and camE 36 ) representing the three diketocamphane monooxygenase (DKCMO) isozymes are highlighted in grey. The previously established OTEMO-encoding gene been designated camG in accordance with the respective catabolic step. Figure reproduced from Iwaki et al.  with permission. American Society for Microbiology, licence number 4487631509592.

    Journal: Microorganisms

    Article Title: The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453—An Episodic History and Still Mysterious after 60 Years

    doi: 10.3390/microorganisms9122593

    Figure Lengend Snippet: Localization of additional genes and predicted open reading frames (ORFs) flanking the established initial genes of the camphor camDCAB operon and its repressor, camR , on an~40.5-kb established sequenced region of the CAM plasmid of P. putida ATCC 17453. The predicted ORFs or genes are sequenced numbered from 1 to 27, except for the established camRDCAB genes, which are shaded in black. The numbered from 1 to 27, except for the established camRDCAB genes, which are shaded in black. The orientation of the arrows indicates the direction of gene transcription. The candidate genes of this study ( camE 25–1 , camE 25–2 , and camE 36 ) representing the three diketocamphane monooxygenase (DKCMO) isozymes are highlighted in grey. The previously established OTEMO-encoding gene been designated camG in accordance with the respective catabolic step. Figure reproduced from Iwaki et al. with permission. American Society for Microbiology, licence number 4487631509592.

    Article Snippet: Now, over 60 years later, it has progressed sufficiently to validate another of Gunsalus’s astute predictions by confirming that the CAM plasmid of P putida ATCC 17453 can function as a totally autonomous metabolic entity.

    Techniques: Plasmid Preparation

    Changes in the optical density (A 500 nm), succinate (mM), ( rac )-camphor (mM), and the specific activity of key enzymes of ( rac )-camphor degradation during diauxic growth of by P. putida ATCC 17453 on succinate plus ( rac )-camphor based defined medium. Camphor hydroxylase = camphor 5-monooxygenase.

    Journal: Microorganisms

    Article Title: The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453—An Episodic History and Still Mysterious after 60 Years

    doi: 10.3390/microorganisms9122593

    Figure Lengend Snippet: Changes in the optical density (A 500 nm), succinate (mM), ( rac )-camphor (mM), and the specific activity of key enzymes of ( rac )-camphor degradation during diauxic growth of by P. putida ATCC 17453 on succinate plus ( rac )-camphor based defined medium. Camphor hydroxylase = camphor 5-monooxygenase.

    Article Snippet: Now, over 60 years later, it has progressed sufficiently to validate another of Gunsalus’s astute predictions by confirming that the CAM plasmid of P putida ATCC 17453 can function as a totally autonomous metabolic entity.

    Techniques: Activity Assay

    Transcriptional controls of the pathway of (+)- and (−)-camphor degradation in P. putida ATCC 17453 ●+ = induction: −● = repression: A = camphor 5-monooxygenase ( camCAB ): B = exo -hydroxycamphor dehydrogenase ( camD ): C = 2,5-diketocamphane 1,2-monooxygenase ( camE 25-1 + camE 25-2 ); D = 3,6-diketo- camphane 1,6-monooxygenase camE 36 ); E = 2-oxo-△ 3 -4,5,5-trimethylcyclo-pentenylacetyl-CoA synthetase ( camF1 + F2) ; F = 2-oxo-△ 3 -4,5,5-trimethylcyclo-pentenylacetyl-CoA monooxygenase ( camG ).

    Journal: Microorganisms

    Article Title: The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453—An Episodic History and Still Mysterious after 60 Years

    doi: 10.3390/microorganisms9122593

    Figure Lengend Snippet: Transcriptional controls of the pathway of (+)- and (−)-camphor degradation in P. putida ATCC 17453 ●+ = induction: −● = repression: A = camphor 5-monooxygenase ( camCAB ): B = exo -hydroxycamphor dehydrogenase ( camD ): C = 2,5-diketocamphane 1,2-monooxygenase ( camE 25-1 + camE 25-2 ); D = 3,6-diketo- camphane 1,6-monooxygenase camE 36 ); E = 2-oxo-△ 3 -4,5,5-trimethylcyclo-pentenylacetyl-CoA synthetase ( camF1 + F2) ; F = 2-oxo-△ 3 -4,5,5-trimethylcyclo-pentenylacetyl-CoA monooxygenase ( camG ).

    Article Snippet: Now, over 60 years later, it has progressed sufficiently to validate another of Gunsalus’s astute predictions by confirming that the CAM plasmid of P putida ATCC 17453 can function as a totally autonomous metabolic entity.

    Techniques:

    The relationships between nonselective and stereoselective enzymes that constitute the pathway for the degradation of ( rac )-camphor to OTE in P. putida ATCC 17453, and the roles of chiral and achiral molecules as substrates and transcriptional regulators. A = induction by (+)-camphor and (−)-camphor, plus product induction by OTE: B = induction by (+)-camphor and (−)-camphor, plus product induction by 2,5-DKC and 3,6-DKC: C = induction by (+)-camphor, (−)-camphor and 2,5-DKC, plus cross-induction by 3,6-DKC, and product induction by OTE: D = ‘from the top’ coordinate induction by (+)-camphor, (−)-camphor, and 3,6-DKC, plus cross-induction by 2,5-DKC, and product induction by OTE: A + B + C + D = ‘from the top’ coordinate induction by (+)-camphor and (−)-camphor.

    Journal: Microorganisms

    Article Title: The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453—An Episodic History and Still Mysterious after 60 Years

    doi: 10.3390/microorganisms9122593

    Figure Lengend Snippet: The relationships between nonselective and stereoselective enzymes that constitute the pathway for the degradation of ( rac )-camphor to OTE in P. putida ATCC 17453, and the roles of chiral and achiral molecules as substrates and transcriptional regulators. A = induction by (+)-camphor and (−)-camphor, plus product induction by OTE: B = induction by (+)-camphor and (−)-camphor, plus product induction by 2,5-DKC and 3,6-DKC: C = induction by (+)-camphor, (−)-camphor and 2,5-DKC, plus cross-induction by 3,6-DKC, and product induction by OTE: D = ‘from the top’ coordinate induction by (+)-camphor, (−)-camphor, and 3,6-DKC, plus cross-induction by 2,5-DKC, and product induction by OTE: A + B + C + D = ‘from the top’ coordinate induction by (+)-camphor and (−)-camphor.

    Article Snippet: Now, over 60 years later, it has progressed sufficiently to validate another of Gunsalus’s astute predictions by confirming that the CAM plasmid of P putida ATCC 17453 can function as a totally autonomous metabolic entity.

    Techniques:

    Key interrelationships between CAM plasmid-coded functions and chromosome-coded functions in ( rac )-camphor-grown P. putida ATCC 17453. black = biomolecules exclusive to CAM plasmid-directed biochemistry: red = biomolecules exclusive to chromosome-directed biochemistry: green = biomolecules common to both plasmid-directed and chromosome-directed biochemistry. DKC = diketocamphane; FNR = FMNH 2. ; cytP540MO = camphor 5-monooxygenase; OTE = 2-oxo-△ 3 -4,5,5-trimethylcyclopentylacetic acid; PdR = putidaredoxin.

    Journal: Microorganisms

    Article Title: The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453—An Episodic History and Still Mysterious after 60 Years

    doi: 10.3390/microorganisms9122593

    Figure Lengend Snippet: Key interrelationships between CAM plasmid-coded functions and chromosome-coded functions in ( rac )-camphor-grown P. putida ATCC 17453. black = biomolecules exclusive to CAM plasmid-directed biochemistry: red = biomolecules exclusive to chromosome-directed biochemistry: green = biomolecules common to both plasmid-directed and chromosome-directed biochemistry. DKC = diketocamphane; FNR = FMNH 2. ; cytP540MO = camphor 5-monooxygenase; OTE = 2-oxo-△ 3 -4,5,5-trimethylcyclopentylacetic acid; PdR = putidaredoxin.

    Article Snippet: Now, over 60 years later, it has progressed sufficiently to validate another of Gunsalus’s astute predictions by confirming that the CAM plasmid of P putida ATCC 17453 can function as a totally autonomous metabolic entity.

    Techniques: Plasmid Preparation

    Changes in the activity of ( A ) camphor 5-monooxygenase (cytP450MO), and ( B ) the combined Frp1 + Frp2 activity in P. putida ATCC 17453 in the presence and absence of 30 µM Zn 2+ added during the diauxic interlude that occurs between the swop from late log growth on succinate to early log growth on ( rac )-camphor.

    Journal: Microorganisms

    Article Title: The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453—An Episodic History and Still Mysterious after 60 Years

    doi: 10.3390/microorganisms9122593

    Figure Lengend Snippet: Changes in the activity of ( A ) camphor 5-monooxygenase (cytP450MO), and ( B ) the combined Frp1 + Frp2 activity in P. putida ATCC 17453 in the presence and absence of 30 µM Zn 2+ added during the diauxic interlude that occurs between the swop from late log growth on succinate to early log growth on ( rac )-camphor.

    Article Snippet: Now, over 60 years later, it has progressed sufficiently to validate another of Gunsalus’s astute predictions by confirming that the CAM plasmid of P putida ATCC 17453 can function as a totally autonomous metabolic entity.

    Techniques: Activity Assay

    Schematic of the two contrasting roles of putidaredoxin reductase (PdR) in camphor-grown P. putida ATCC 17453. ( A ). cytP450MO (camphor 5-monooxygenase): Pdx (putidaredoxin) ( B ). 2,5-DKCMO (2,5-diketocamphane 1,2-monooxygenase): DKC (2,5-diketocamphane).

    Journal: Microorganisms

    Article Title: The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453—An Episodic History and Still Mysterious after 60 Years

    doi: 10.3390/microorganisms9122593

    Figure Lengend Snippet: Schematic of the two contrasting roles of putidaredoxin reductase (PdR) in camphor-grown P. putida ATCC 17453. ( A ). cytP450MO (camphor 5-monooxygenase): Pdx (putidaredoxin) ( B ). 2,5-DKCMO (2,5-diketocamphane 1,2-monooxygenase): DKC (2,5-diketocamphane).

    Article Snippet: Now, over 60 years later, it has progressed sufficiently to validate another of Gunsalus’s astute predictions by confirming that the CAM plasmid of P putida ATCC 17453 can function as a totally autonomous metabolic entity.

    Techniques: