Journal: Nature chemical biology
Article Title: Metagenomic discovery of polybrominated diphenyl ether biosynthesis by marine sponges
Figure Lengend Snippet: Host and symbiont phylogenies ( A ) Left: Midpoint-rooted, maximum likelihood (ML) phylogeny based on sponge ribosomal ITS-2 sequences from the current study (in bold) along with representative literature examples. Identical sequences are listed at a single branch tip, with symbols denoting dominant natural product chemistry. Bootstrap values ≥70 are shown; scale bar indicates substitutions per site. Matching superscripts indicate literature ITS-2 and 16S rRNA sequences obtained from the same sponge specimen. Right: H. spongeliae ML tree of near-full length 16S rRNA gene sequences, including closest free-living relative Oscillatoria corallinae , and rooted on closest relative with a sequenced genome, Moorea producens 3L. One full-length 16S rRNA gene sequence (GUM098) was obtained from clone library (see Figure 3 ). Remaining Clade IV 16S rRNA sequences in this study, denoted in parentheses, are represented by 370 bp sequences obtained from high-throughput community data that are 100% identical to the GUM098 full-length sequence. ( B ) Chemical profiles, as monitored by absorbance at 280 nm by HPLC with in situ photographs of representative sponges for each clade.
Article Snippet: To gain insights into the microbial community composition of the different sponges, we inventoried the microbiomes of two specimens from each of Clades Ia, Ib, III, and IV by 16S rRNA gene amplification and deep Illumina sequencing, generating between 75,000-250,000 reads per sample ( ).
Techniques: Sequencing, High Throughput Screening Assay, High Performance Liquid Chromatography, In Situ