15823 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    MedChemExpress cay10566
    Apoptosis induced by SCD inhibition, or treatment with palmitic acid, is attenuated by exogenous oleic acid or SCD overexpression. (A-B) Time-lapse imaging of Annexin V staining to measure apoptosis in OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNA targeting SCD (shSCD), cultured in low serum conditions, and treated with 52μM oleic acid (A), or with 50μM palmitic acid alone or in combination with 52μM oleic acid (B). (C-D) Time-lapse of Annexin V imaging to measure apoptosis in OVCAR-5 cells cultured in low serum conditions and treated with 21.6nM <t>CAY10566,</t> 52μM oleic acid or combination (C), or with 8.1nM CAY10566, 50μM palmitic acid, 52μM oleic acid, or combinations (D). (E) Western blot of full-length and cleaved caspase-3 in shSCD vs shCtrl cells cultured under low serum medium and treated with 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (F) Western blot of full-length and cleaved caspase-3 in OVCAR-5 cells cultured in low serum medium and treated with 8.1nM CAY10566, 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (G) Time-lapse of Annexin V imaging to determine apoptosis in OVCAR-5 cells overexpressing SCD (pLenti-SCD) and control cells (pLenti-Ctrl), cultured in medium containing low serum and treated with 50μM palmitic acid. Values in panels A-D G are means ± SD, n = 6. * p
    Cay10566, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cay10566/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cay10566 - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    93
    Abcam anti histone h4k8ac ab 15823 antibodies
    Apoptosis induced by SCD inhibition, or treatment with palmitic acid, is attenuated by exogenous oleic acid or SCD overexpression. (A-B) Time-lapse imaging of Annexin V staining to measure apoptosis in OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNA targeting SCD (shSCD), cultured in low serum conditions, and treated with 52μM oleic acid (A), or with 50μM palmitic acid alone or in combination with 52μM oleic acid (B). (C-D) Time-lapse of Annexin V imaging to measure apoptosis in OVCAR-5 cells cultured in low serum conditions and treated with 21.6nM <t>CAY10566,</t> 52μM oleic acid or combination (C), or with 8.1nM CAY10566, 50μM palmitic acid, 52μM oleic acid, or combinations (D). (E) Western blot of full-length and cleaved caspase-3 in shSCD vs shCtrl cells cultured under low serum medium and treated with 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (F) Western blot of full-length and cleaved caspase-3 in OVCAR-5 cells cultured in low serum medium and treated with 8.1nM CAY10566, 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (G) Time-lapse of Annexin V imaging to determine apoptosis in OVCAR-5 cells overexpressing SCD (pLenti-SCD) and control cells (pLenti-Ctrl), cultured in medium containing low serum and treated with 50μM palmitic acid. Values in panels A-D G are means ± SD, n = 6. * p
    Anti Histone H4k8ac Ab 15823 Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti histone h4k8ac ab 15823 antibodies/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti histone h4k8ac ab 15823 antibodies - by Bioz Stars, 2022-12
    93/100 stars
      Buy from Supplier

    91
    Addgene inc pcdna3 dn hcul5 flag plasmid 15823
    Apoptosis induced by SCD inhibition, or treatment with palmitic acid, is attenuated by exogenous oleic acid or SCD overexpression. (A-B) Time-lapse imaging of Annexin V staining to measure apoptosis in OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNA targeting SCD (shSCD), cultured in low serum conditions, and treated with 52μM oleic acid (A), or with 50μM palmitic acid alone or in combination with 52μM oleic acid (B). (C-D) Time-lapse of Annexin V imaging to measure apoptosis in OVCAR-5 cells cultured in low serum conditions and treated with 21.6nM <t>CAY10566,</t> 52μM oleic acid or combination (C), or with 8.1nM CAY10566, 50μM palmitic acid, 52μM oleic acid, or combinations (D). (E) Western blot of full-length and cleaved caspase-3 in shSCD vs shCtrl cells cultured under low serum medium and treated with 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (F) Western blot of full-length and cleaved caspase-3 in OVCAR-5 cells cultured in low serum medium and treated with 8.1nM CAY10566, 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (G) Time-lapse of Annexin V imaging to determine apoptosis in OVCAR-5 cells overexpressing SCD (pLenti-SCD) and control cells (pLenti-Ctrl), cultured in medium containing low serum and treated with 50μM palmitic acid. Values in panels A-D G are means ± SD, n = 6. * p
    Pcdna3 Dn Hcul5 Flag Plasmid 15823, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna3 dn hcul5 flag plasmid 15823/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcdna3 dn hcul5 flag plasmid 15823 - by Bioz Stars, 2022-12
    91/100 stars
      Buy from Supplier

    Image Search Results


    Apoptosis induced by SCD inhibition, or treatment with palmitic acid, is attenuated by exogenous oleic acid or SCD overexpression. (A-B) Time-lapse imaging of Annexin V staining to measure apoptosis in OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNA targeting SCD (shSCD), cultured in low serum conditions, and treated with 52μM oleic acid (A), or with 50μM palmitic acid alone or in combination with 52μM oleic acid (B). (C-D) Time-lapse of Annexin V imaging to measure apoptosis in OVCAR-5 cells cultured in low serum conditions and treated with 21.6nM CAY10566, 52μM oleic acid or combination (C), or with 8.1nM CAY10566, 50μM palmitic acid, 52μM oleic acid, or combinations (D). (E) Western blot of full-length and cleaved caspase-3 in shSCD vs shCtrl cells cultured under low serum medium and treated with 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (F) Western blot of full-length and cleaved caspase-3 in OVCAR-5 cells cultured in low serum medium and treated with 8.1nM CAY10566, 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (G) Time-lapse of Annexin V imaging to determine apoptosis in OVCAR-5 cells overexpressing SCD (pLenti-SCD) and control cells (pLenti-Ctrl), cultured in medium containing low serum and treated with 50μM palmitic acid. Values in panels A-D G are means ± SD, n = 6. * p

    Journal: bioRxiv

    Article Title: The Balance between Saturated and Unsaturated Fatty Acids Regulates Ovarian Cancer Cell Fate

    doi: 10.1101/2022.05.24.493247

    Figure Lengend Snippet: Apoptosis induced by SCD inhibition, or treatment with palmitic acid, is attenuated by exogenous oleic acid or SCD overexpression. (A-B) Time-lapse imaging of Annexin V staining to measure apoptosis in OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNA targeting SCD (shSCD), cultured in low serum conditions, and treated with 52μM oleic acid (A), or with 50μM palmitic acid alone or in combination with 52μM oleic acid (B). (C-D) Time-lapse of Annexin V imaging to measure apoptosis in OVCAR-5 cells cultured in low serum conditions and treated with 21.6nM CAY10566, 52μM oleic acid or combination (C), or with 8.1nM CAY10566, 50μM palmitic acid, 52μM oleic acid, or combinations (D). (E) Western blot of full-length and cleaved caspase-3 in shSCD vs shCtrl cells cultured under low serum medium and treated with 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (F) Western blot of full-length and cleaved caspase-3 in OVCAR-5 cells cultured in low serum medium and treated with 8.1nM CAY10566, 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (G) Time-lapse of Annexin V imaging to determine apoptosis in OVCAR-5 cells overexpressing SCD (pLenti-SCD) and control cells (pLenti-Ctrl), cultured in medium containing low serum and treated with 50μM palmitic acid. Values in panels A-D G are means ± SD, n = 6. * p

    Article Snippet: CAY10566 (cat#: HY-15823) and PEG300 (cat#: HY-Y0873) were purchased from MedChemExpress.

    Techniques: Inhibition, Over Expression, Imaging, Staining, Transduction, shRNA, Cell Culture, Western Blot

    The IRE1α/XBP1 and PERK/eIF2α/ATF4 axes of the ER stress response pathway are activated in ovarian cancer cells under restricted availability of unsaturated fatty acid. (A) XBP1 splicing (u, unspliced transcript; s, spliced transcript) measured by RT-PCR and agarose-gel electrophoresis in OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNAs (1 or 2) targeting SCD (shSCD) and cultured in low serum conditions (1% FBS) for 48 hours. (B) Densitometric analysis of XBP1 splicing products shown in A. Bars represent percent of spliced XBP1 relative to total XBP1 (mean ± SD, n = 3). (C) XBP1 splicing (u, unspliced transcript; s, spliced transcript) in OVCAR-5 cells cultured in low serum conditions and treated with SCD inhibitor CAY10566 for 48 hours. (D) Percent of spliced XBP1 isoform measured by densitometric analysis of PCR products shown in C (mean ± SD, n = 3). (E, F) XBP1 splicing (u, unspliced transcript; s, spliced transcript) (E), and western blot of proteins of the PERK/eIF2α/ATF4 axis (F) in shCtrl and shSCD OVCAR-5 cells cultured under low serum conditions and treated with 50μM palmitic acid for the time periods indicated. (G) XBP1 splicing (u, unspliced transcript; s, spliced transcript) in primary cells from tumors of ovarian cancer patients cultured in low serum conditions and treated with 3μM CAY10566 for 48 hours, or with 1μM CAY10566 and 50μM palmitic acid for 12 hours. (H) Representative SRS images in the C-H and C-D regions of OVCAR-5 shCtrl and shSCD cells cultured in low serum conditions (1% FBS) and treated with 12.5μM palmitic acid-d31 (PA-d31), with or without 52μM oleic acid (OA), or cultured in medium with full serum (10% FBS) and treated with PA-d31 for 24 hr. Yellow arrows indicate rigid ER, gray arrows indicate lipid droplet (LD), and blue arrows indicate cytoplasm. Scale bar: 20 µm. (I) Percentages of shCtrl and shSCD OVCAR-5 cells treated as described in (H) showing C-D SRS signal mainly in rigid ER, lipid droplet (LD), and cytoplasm (n = 139-191). (J) Transmission electron microscopy imaging of smooth ER (red arrows) in OVCAR-5 shCtrl vs shSCD cells cultured in low serum conditions for 48 hours. * p

    Journal: bioRxiv

    Article Title: The Balance between Saturated and Unsaturated Fatty Acids Regulates Ovarian Cancer Cell Fate

    doi: 10.1101/2022.05.24.493247

    Figure Lengend Snippet: The IRE1α/XBP1 and PERK/eIF2α/ATF4 axes of the ER stress response pathway are activated in ovarian cancer cells under restricted availability of unsaturated fatty acid. (A) XBP1 splicing (u, unspliced transcript; s, spliced transcript) measured by RT-PCR and agarose-gel electrophoresis in OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNAs (1 or 2) targeting SCD (shSCD) and cultured in low serum conditions (1% FBS) for 48 hours. (B) Densitometric analysis of XBP1 splicing products shown in A. Bars represent percent of spliced XBP1 relative to total XBP1 (mean ± SD, n = 3). (C) XBP1 splicing (u, unspliced transcript; s, spliced transcript) in OVCAR-5 cells cultured in low serum conditions and treated with SCD inhibitor CAY10566 for 48 hours. (D) Percent of spliced XBP1 isoform measured by densitometric analysis of PCR products shown in C (mean ± SD, n = 3). (E, F) XBP1 splicing (u, unspliced transcript; s, spliced transcript) (E), and western blot of proteins of the PERK/eIF2α/ATF4 axis (F) in shCtrl and shSCD OVCAR-5 cells cultured under low serum conditions and treated with 50μM palmitic acid for the time periods indicated. (G) XBP1 splicing (u, unspliced transcript; s, spliced transcript) in primary cells from tumors of ovarian cancer patients cultured in low serum conditions and treated with 3μM CAY10566 for 48 hours, or with 1μM CAY10566 and 50μM palmitic acid for 12 hours. (H) Representative SRS images in the C-H and C-D regions of OVCAR-5 shCtrl and shSCD cells cultured in low serum conditions (1% FBS) and treated with 12.5μM palmitic acid-d31 (PA-d31), with or without 52μM oleic acid (OA), or cultured in medium with full serum (10% FBS) and treated with PA-d31 for 24 hr. Yellow arrows indicate rigid ER, gray arrows indicate lipid droplet (LD), and blue arrows indicate cytoplasm. Scale bar: 20 µm. (I) Percentages of shCtrl and shSCD OVCAR-5 cells treated as described in (H) showing C-D SRS signal mainly in rigid ER, lipid droplet (LD), and cytoplasm (n = 139-191). (J) Transmission electron microscopy imaging of smooth ER (red arrows) in OVCAR-5 shCtrl vs shSCD cells cultured in low serum conditions for 48 hours. * p

    Article Snippet: CAY10566 (cat#: HY-15823) and PEG300 (cat#: HY-Y0873) were purchased from MedChemExpress.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Transduction, shRNA, Cell Culture, Polymerase Chain Reaction, Western Blot, Transmission Assay, Electron Microscopy, Imaging

    Unsaturated fatty acid-induced ER stress response is reversed by exogenous oleic acid. (A, B) XBP1 splicing (u, unspliced transcript; s, spliced transcript) measured by RT-PCR and agarose gel electrophoresis in OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNAs (1 or 2) targeting SCD (shSCD), cultured in medium containing low serum, and treated with indicated doses of oleic acid for 48 hours (A), or with 50μM palmitic acid and indicated doses of oleic acid for 12 hours (B). (C, D) Western blot of SCD and proteins of the PERK/eIF2α/ATF4 axis in shCtrl and shSCD cells cultured in low serum medium, and treated with different doses of oleic acid for 48 hours (C), or with 50μM palmitic acid and indicated doses of oleic acid for 12 hours (D). (E, F) XBP1 splicing (u, unspliced transcript; s, spliced transcript) in OVCAR-5 cells cultured in medium containing low serum and treated with 21.6nM CAY10566 and indicated doses of oleic acid for 48 hours (E), or with 8.1nM CAY10566, 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (G) Proteins of the PERK/eIF2α/ATF4 pathway measured by western blot in OVCAR-5 cells treated as described in (E). (H) Western blot of proteins of the PERK/eIF2α/ATF4 pathway in OVCAR-5 cells treated as described in (F). Arrows indicate the band of interest. (I, J) Verification of SCD overexpression by qRT-PCR (I) and western blotting (J) in OVCAR-5 cells transduced with a SCD expression vector (pLenti-SCD). Cells transduced with empty vector (pLenti-Ctrl) served as control. Bars represent means ± SD, n = 3. (K) XBP1 splicing (u, unspliced transcript; s, spliced transcript) in pLenti-SCD vs pLenti-Ctrl cells cultured in low serum conditions and treated with 50μM palmitic acid for 12 hours. (M) Percent of spliced isoform (mean ± SD, n = 3) calculated by densitometric analysis of PCR products shown in (L). * p

    Journal: bioRxiv

    Article Title: The Balance between Saturated and Unsaturated Fatty Acids Regulates Ovarian Cancer Cell Fate

    doi: 10.1101/2022.05.24.493247

    Figure Lengend Snippet: Unsaturated fatty acid-induced ER stress response is reversed by exogenous oleic acid. (A, B) XBP1 splicing (u, unspliced transcript; s, spliced transcript) measured by RT-PCR and agarose gel electrophoresis in OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNAs (1 or 2) targeting SCD (shSCD), cultured in medium containing low serum, and treated with indicated doses of oleic acid for 48 hours (A), or with 50μM palmitic acid and indicated doses of oleic acid for 12 hours (B). (C, D) Western blot of SCD and proteins of the PERK/eIF2α/ATF4 axis in shCtrl and shSCD cells cultured in low serum medium, and treated with different doses of oleic acid for 48 hours (C), or with 50μM palmitic acid and indicated doses of oleic acid for 12 hours (D). (E, F) XBP1 splicing (u, unspliced transcript; s, spliced transcript) in OVCAR-5 cells cultured in medium containing low serum and treated with 21.6nM CAY10566 and indicated doses of oleic acid for 48 hours (E), or with 8.1nM CAY10566, 50μM palmitic acid and indicated doses of oleic acid for 12 hours. (G) Proteins of the PERK/eIF2α/ATF4 pathway measured by western blot in OVCAR-5 cells treated as described in (E). (H) Western blot of proteins of the PERK/eIF2α/ATF4 pathway in OVCAR-5 cells treated as described in (F). Arrows indicate the band of interest. (I, J) Verification of SCD overexpression by qRT-PCR (I) and western blotting (J) in OVCAR-5 cells transduced with a SCD expression vector (pLenti-SCD). Cells transduced with empty vector (pLenti-Ctrl) served as control. Bars represent means ± SD, n = 3. (K) XBP1 splicing (u, unspliced transcript; s, spliced transcript) in pLenti-SCD vs pLenti-Ctrl cells cultured in low serum conditions and treated with 50μM palmitic acid for 12 hours. (M) Percent of spliced isoform (mean ± SD, n = 3) calculated by densitometric analysis of PCR products shown in (L). * p

    Article Snippet: CAY10566 (cat#: HY-15823) and PEG300 (cat#: HY-Y0873) were purchased from MedChemExpress.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Transduction, shRNA, Cell Culture, Western Blot, Over Expression, Quantitative RT-PCR, Expressing, Plasmid Preparation, Polymerase Chain Reaction

    SCD knockdown inhibited growth of ovarian cancer xenografts in mice. (A-C) Total tumor weight (A), total tumor volume (B) and total number of metastases (C) in athymic nude mice intraperitoneally injected with OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNA targeting SCD (shSCD), and evaluated after 28 days (values are means ± SE, n = 14 per group). (D) qRT-PCR measurements of SCD expression (mean ± SD, n = 6) in a random sample of tumor xenografts described in (A). (E, F) Agarose gel electrophoresis of XBP1 splicing products (u, unspliced transcript; s, spliced transcript) (E), and percent spliced XBP1 isoform estimated by image analysis of transcript bands (mean ± SD, n = 6) (F) in a random sample of tumor xenografts described in (A). (G, H) Ascites volume (G) and total number of metastases (H) in athymic nude mice intraperitoneally injected with OVCAR-5 cells, fed with a palmitic acid-rich diet or control diet, and treated with SCD inhibitor CAY10566 or vehicle for 28 days. Values are means ± SE, n = 10. (I-L) XBP1 splicing products (u, unspliced transcript; s, spliced transcript) (I, K), and percent intensity of the spliced transcript estimated by image analysis (J, L) in a random sample (n = 5) of tumor xenografts described in (F). Values are means ± SD. * p

    Journal: bioRxiv

    Article Title: The Balance between Saturated and Unsaturated Fatty Acids Regulates Ovarian Cancer Cell Fate

    doi: 10.1101/2022.05.24.493247

    Figure Lengend Snippet: SCD knockdown inhibited growth of ovarian cancer xenografts in mice. (A-C) Total tumor weight (A), total tumor volume (B) and total number of metastases (C) in athymic nude mice intraperitoneally injected with OVCAR-5 cells transduced with control shRNA (shCtrl) or shRNA targeting SCD (shSCD), and evaluated after 28 days (values are means ± SE, n = 14 per group). (D) qRT-PCR measurements of SCD expression (mean ± SD, n = 6) in a random sample of tumor xenografts described in (A). (E, F) Agarose gel electrophoresis of XBP1 splicing products (u, unspliced transcript; s, spliced transcript) (E), and percent spliced XBP1 isoform estimated by image analysis of transcript bands (mean ± SD, n = 6) (F) in a random sample of tumor xenografts described in (A). (G, H) Ascites volume (G) and total number of metastases (H) in athymic nude mice intraperitoneally injected with OVCAR-5 cells, fed with a palmitic acid-rich diet or control diet, and treated with SCD inhibitor CAY10566 or vehicle for 28 days. Values are means ± SE, n = 10. (I-L) XBP1 splicing products (u, unspliced transcript; s, spliced transcript) (I, K), and percent intensity of the spliced transcript estimated by image analysis (J, L) in a random sample (n = 5) of tumor xenografts described in (F). Values are means ± SD. * p

    Article Snippet: CAY10566 (cat#: HY-15823) and PEG300 (cat#: HY-Y0873) were purchased from MedChemExpress.

    Techniques: Mouse Assay, Injection, Transduction, shRNA, Quantitative RT-PCR, Expressing, Agarose Gel Electrophoresis