15697 Search Results


99
ATCC b longum subsp infantis atcc 15697
B Longum Subsp Infantis Atcc 15697, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Proteintech rabbit anti prm1
Rabbit Anti Prm1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Proteintech prm1 rabbit pab
BAF-L is implicated in the exchange of histones and protamines in spermiogenesis. ( A ) Immunoprecipitation analysis performed with BAF-L antibody displays the interaction of BAF-L with histones H3 and H4 and <t>protamine</t> <t>1</t> but not histones H2A nor H2B. ( B ) Immunoprecipitation analysis performed with histone H3 antibody displays the interaction of BAF-L with histone H3. ( C ) Immunoprecipitation analysis performed with histone H4 antibody displays the interaction of BAF-L with histone H4. ( D ) Immunoprecipitation analysis performed with protamine 1 antibody displays the interaction of BAF-L with histone protamine 1. ( E , F ) Immunofluorescence staining and quantification show increased labeling of histones H3 and H4 in elongating/elongate spermatids of 5-week-old BAF-L KO testis. White arrows indicate normal elongating/elongated spermatids, while purple arrows indicate elongating/elongated spermatids with histones. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 6 for WT and n = 5 for KO. ( G ) qRT-PCR shows decreased mRNA expression of Tnp1, Tnp2, <t>Prm1,</t> and Prm2 in 5-week-old BAF-L KO testis. Error bars indicate SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 by two-tailed Student’s t -test. n = 4. ( H , I ) Representative images of aniline blue staining and quantification show increased ratio of spermatozoa with blue stain in BAL-L KO spermatozoa. Green arrows indicate normal spermatozoa, while red arrows indicate spermatozoa with histones. Error bars indicate SD. *** p < 0.001 by two-tailed Student’s t -test. ( J – L ) Western blots and quantification show decreased protein level of Acetyl-histone H3 and Acetyl-histone H4 in BAF-L KO testis. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 4.
Prm1 Rabbit Pab, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Chemie GmbH angewandte chemie communications 15697 angew
BAF-L is implicated in the exchange of histones and protamines in spermiogenesis. ( A ) Immunoprecipitation analysis performed with BAF-L antibody displays the interaction of BAF-L with histones H3 and H4 and <t>protamine</t> <t>1</t> but not histones H2A nor H2B. ( B ) Immunoprecipitation analysis performed with histone H3 antibody displays the interaction of BAF-L with histone H3. ( C ) Immunoprecipitation analysis performed with histone H4 antibody displays the interaction of BAF-L with histone H4. ( D ) Immunoprecipitation analysis performed with protamine 1 antibody displays the interaction of BAF-L with histone protamine 1. ( E , F ) Immunofluorescence staining and quantification show increased labeling of histones H3 and H4 in elongating/elongate spermatids of 5-week-old BAF-L KO testis. White arrows indicate normal elongating/elongated spermatids, while purple arrows indicate elongating/elongated spermatids with histones. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 6 for WT and n = 5 for KO. ( G ) qRT-PCR shows decreased mRNA expression of Tnp1, Tnp2, <t>Prm1,</t> and Prm2 in 5-week-old BAF-L KO testis. Error bars indicate SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 by two-tailed Student’s t -test. n = 4. ( H , I ) Representative images of aniline blue staining and quantification show increased ratio of spermatozoa with blue stain in BAL-L KO spermatozoa. Green arrows indicate normal spermatozoa, while red arrows indicate spermatozoa with histones. Error bars indicate SD. *** p < 0.001 by two-tailed Student’s t -test. ( J – L ) Western blots and quantification show decreased protein level of Acetyl-histone H3 and Acetyl-histone H4 in BAF-L KO testis. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 4.
Angewandte Chemie Communications 15697 Angew, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Leica Microsystems application suite x software 3 0 0 15697
BAF-L is implicated in the exchange of histones and protamines in spermiogenesis. ( A ) Immunoprecipitation analysis performed with BAF-L antibody displays the interaction of BAF-L with histones H3 and H4 and <t>protamine</t> <t>1</t> but not histones H2A nor H2B. ( B ) Immunoprecipitation analysis performed with histone H3 antibody displays the interaction of BAF-L with histone H3. ( C ) Immunoprecipitation analysis performed with histone H4 antibody displays the interaction of BAF-L with histone H4. ( D ) Immunoprecipitation analysis performed with protamine 1 antibody displays the interaction of BAF-L with histone protamine 1. ( E , F ) Immunofluorescence staining and quantification show increased labeling of histones H3 and H4 in elongating/elongate spermatids of 5-week-old BAF-L KO testis. White arrows indicate normal elongating/elongated spermatids, while purple arrows indicate elongating/elongated spermatids with histones. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 6 for WT and n = 5 for KO. ( G ) qRT-PCR shows decreased mRNA expression of Tnp1, Tnp2, <t>Prm1,</t> and Prm2 in 5-week-old BAF-L KO testis. Error bars indicate SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 by two-tailed Student’s t -test. n = 4. ( H , I ) Representative images of aniline blue staining and quantification show increased ratio of spermatozoa with blue stain in BAL-L KO spermatozoa. Green arrows indicate normal spermatozoa, while red arrows indicate spermatozoa with histones. Error bars indicate SD. *** p < 0.001 by two-tailed Student’s t -test. ( J – L ) Western blots and quantification show decreased protein level of Acetyl-histone H3 and Acetyl-histone H4 in BAF-L KO testis. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 4.
Application Suite X Software 3 0 0 15697, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


BAF-L is implicated in the exchange of histones and protamines in spermiogenesis. ( A ) Immunoprecipitation analysis performed with BAF-L antibody displays the interaction of BAF-L with histones H3 and H4 and protamine 1 but not histones H2A nor H2B. ( B ) Immunoprecipitation analysis performed with histone H3 antibody displays the interaction of BAF-L with histone H3. ( C ) Immunoprecipitation analysis performed with histone H4 antibody displays the interaction of BAF-L with histone H4. ( D ) Immunoprecipitation analysis performed with protamine 1 antibody displays the interaction of BAF-L with histone protamine 1. ( E , F ) Immunofluorescence staining and quantification show increased labeling of histones H3 and H4 in elongating/elongate spermatids of 5-week-old BAF-L KO testis. White arrows indicate normal elongating/elongated spermatids, while purple arrows indicate elongating/elongated spermatids with histones. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 6 for WT and n = 5 for KO. ( G ) qRT-PCR shows decreased mRNA expression of Tnp1, Tnp2, Prm1, and Prm2 in 5-week-old BAF-L KO testis. Error bars indicate SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 by two-tailed Student’s t -test. n = 4. ( H , I ) Representative images of aniline blue staining and quantification show increased ratio of spermatozoa with blue stain in BAL-L KO spermatozoa. Green arrows indicate normal spermatozoa, while red arrows indicate spermatozoa with histones. Error bars indicate SD. *** p < 0.001 by two-tailed Student’s t -test. ( J – L ) Western blots and quantification show decreased protein level of Acetyl-histone H3 and Acetyl-histone H4 in BAF-L KO testis. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 4.

Journal: International Journal of Molecular Sciences

Article Title: BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis

doi: 10.3390/ijms23041985

Figure Lengend Snippet: BAF-L is implicated in the exchange of histones and protamines in spermiogenesis. ( A ) Immunoprecipitation analysis performed with BAF-L antibody displays the interaction of BAF-L with histones H3 and H4 and protamine 1 but not histones H2A nor H2B. ( B ) Immunoprecipitation analysis performed with histone H3 antibody displays the interaction of BAF-L with histone H3. ( C ) Immunoprecipitation analysis performed with histone H4 antibody displays the interaction of BAF-L with histone H4. ( D ) Immunoprecipitation analysis performed with protamine 1 antibody displays the interaction of BAF-L with histone protamine 1. ( E , F ) Immunofluorescence staining and quantification show increased labeling of histones H3 and H4 in elongating/elongate spermatids of 5-week-old BAF-L KO testis. White arrows indicate normal elongating/elongated spermatids, while purple arrows indicate elongating/elongated spermatids with histones. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 6 for WT and n = 5 for KO. ( G ) qRT-PCR shows decreased mRNA expression of Tnp1, Tnp2, Prm1, and Prm2 in 5-week-old BAF-L KO testis. Error bars indicate SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 by two-tailed Student’s t -test. n = 4. ( H , I ) Representative images of aniline blue staining and quantification show increased ratio of spermatozoa with blue stain in BAL-L KO spermatozoa. Green arrows indicate normal spermatozoa, while red arrows indicate spermatozoa with histones. Error bars indicate SD. *** p < 0.001 by two-tailed Student’s t -test. ( J – L ) Western blots and quantification show decreased protein level of Acetyl-histone H3 and Acetyl-histone H4 in BAF-L KO testis. Error bars indicate SD. ** p < 0.01 by two-tailed Student’s t -test. n = 4.

Article Snippet: PRM1 Rabbit pAb , Proteintech , Cat#15697-1-AP WB: 1:1000.

Techniques: Immunoprecipitation, Immunofluorescence, Staining, Labeling, Two Tailed Test, Quantitative RT-PCR, Expressing, Western Blot

Antibodies used in this study.

Journal: International Journal of Molecular Sciences

Article Title: BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis

doi: 10.3390/ijms23041985

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: PRM1 Rabbit pAb , Proteintech , Cat#15697-1-AP WB: 1:1000.

Techniques: Generated