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  • 99
    ATCC a10 cells
    A10 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a10 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a10 cells - by Bioz Stars, 2024-02
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    93
    ATCC p acanthamoebae bn 9
    P Acanthamoebae Bn 9, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p acanthamoebae bn 9/product/ATCC
    Average 93 stars, based on 1 article reviews
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    p acanthamoebae bn 9 - by Bioz Stars, 2024-02
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    90
    ATCC pbluescript sk 1
    Pbluescript Sk 1, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbluescript sk 1/product/ATCC
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    93
    Addgene inc full length chk1
    Full Length Chk1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full length chk1/product/Addgene inc
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    92
    Tocris gap27
    Inhibition of Cx43-GJIC recapitulates sofb123 phenotypes. (A) Schematic of Cx43 showing the location of <t>Gap27</t> interaction with the second extracellular loop. N, amino terminus; C, carboxy terminus; EL1/EL2, extracellular loop1/2; CL, cytoplasmic loop. (B) Representative longitudinal cross-sections showing localization of 5FAM-Gap27 (green) following its injection. DAPI (blue) stains cell nuclei. m, mesenchyme; e, epidermis. UN, uninjected. (C) Regenerate length is reduced in Gap27-injected fins compared with Scrm-Gap27-injected fins. All fins were amputated at the 50% level. The amputation plane is indicated (black line). Black arrow indicates the distance from the amputation plane to the distal tip of the third fin ray. Injections were performed at 3 dpa and measurements were taken at 7 dpa. UN, uninjected. Bar graph (F) shows a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27 (one-way ANOVA with Tukey's and Dunnett's multiple comparison as post-hoc tests; *P<0.05). n=21; three independent replicates. (D) Calcein-stained fin rays show reduced segment length in Gap27-injected fins compared with Scrm-Gap27-injected fins. Arrows and arrowheads point to the first two joints following the amputation plane (white line). The inset shows a higher magnification of the segments. Bar graph (G) reveals a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27 (one-way ANOVA with Tukey's and Dunnett's multiple comparison as post-hoc tests; *P<0.05). n=19; three independent replicates. (E) H3P+ cells are reduced in number in Gap27-injected fins compared with Scrm-Gap27-injected fins. Arrowheads identify one H3P+ cell per image. Bar graph (H) reveals a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27. Student's t-test, two tailed and unpaired, *P<0.05 (n=23, three independent replicates). Data are mean±s.e.m.
    Gap27, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    gap27 - by Bioz Stars, 2024-02
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    Image Search Results


    Inhibition of Cx43-GJIC recapitulates sofb123 phenotypes. (A) Schematic of Cx43 showing the location of Gap27 interaction with the second extracellular loop. N, amino terminus; C, carboxy terminus; EL1/EL2, extracellular loop1/2; CL, cytoplasmic loop. (B) Representative longitudinal cross-sections showing localization of 5FAM-Gap27 (green) following its injection. DAPI (blue) stains cell nuclei. m, mesenchyme; e, epidermis. UN, uninjected. (C) Regenerate length is reduced in Gap27-injected fins compared with Scrm-Gap27-injected fins. All fins were amputated at the 50% level. The amputation plane is indicated (black line). Black arrow indicates the distance from the amputation plane to the distal tip of the third fin ray. Injections were performed at 3 dpa and measurements were taken at 7 dpa. UN, uninjected. Bar graph (F) shows a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27 (one-way ANOVA with Tukey's and Dunnett's multiple comparison as post-hoc tests; *P<0.05). n=21; three independent replicates. (D) Calcein-stained fin rays show reduced segment length in Gap27-injected fins compared with Scrm-Gap27-injected fins. Arrows and arrowheads point to the first two joints following the amputation plane (white line). The inset shows a higher magnification of the segments. Bar graph (G) reveals a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27 (one-way ANOVA with Tukey's and Dunnett's multiple comparison as post-hoc tests; *P<0.05). n=19; three independent replicates. (E) H3P+ cells are reduced in number in Gap27-injected fins compared with Scrm-Gap27-injected fins. Arrowheads identify one H3P+ cell per image. Bar graph (H) reveals a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27. Student's t-test, two tailed and unpaired, *P<0.05 (n=23, three independent replicates). Data are mean±s.e.m.

    Journal: Development (Cambridge, England)

    Article Title: Connexin 43 gap junctional intercellular communication inhibits evx1 expression and joint formation in regenerating fins

    doi: 10.1242/dev.190512

    Figure Lengend Snippet: Inhibition of Cx43-GJIC recapitulates sofb123 phenotypes. (A) Schematic of Cx43 showing the location of Gap27 interaction with the second extracellular loop. N, amino terminus; C, carboxy terminus; EL1/EL2, extracellular loop1/2; CL, cytoplasmic loop. (B) Representative longitudinal cross-sections showing localization of 5FAM-Gap27 (green) following its injection. DAPI (blue) stains cell nuclei. m, mesenchyme; e, epidermis. UN, uninjected. (C) Regenerate length is reduced in Gap27-injected fins compared with Scrm-Gap27-injected fins. All fins were amputated at the 50% level. The amputation plane is indicated (black line). Black arrow indicates the distance from the amputation plane to the distal tip of the third fin ray. Injections were performed at 3 dpa and measurements were taken at 7 dpa. UN, uninjected. Bar graph (F) shows a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27 (one-way ANOVA with Tukey's and Dunnett's multiple comparison as post-hoc tests; *P<0.05). n=21; three independent replicates. (D) Calcein-stained fin rays show reduced segment length in Gap27-injected fins compared with Scrm-Gap27-injected fins. Arrows and arrowheads point to the first two joints following the amputation plane (white line). The inset shows a higher magnification of the segments. Bar graph (G) reveals a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27 (one-way ANOVA with Tukey's and Dunnett's multiple comparison as post-hoc tests; *P<0.05). n=19; three independent replicates. (E) H3P+ cells are reduced in number in Gap27-injected fins compared with Scrm-Gap27-injected fins. Arrowheads identify one H3P+ cell per image. Bar graph (H) reveals a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27. Student's t-test, two tailed and unpaired, *P<0.05 (n=23, three independent replicates). Data are mean±s.e.m.

    Article Snippet: Peptide injections into regenerating fins Peptides used in this study include: Gap27 (Tocris, 1476), 5Fam-Gap27, scrambled Gap27 peptide and TAT-Gap19 (YGRKKRRQRRR-KQIEIKKFK, Tocris, 6227).

    Techniques: Inhibition, Injection, Staining, Two Tailed Test

    Cx43-GJIC and Smp act in a common pathway. (A) Two previously described Cx43-dependent pathways impinging on joint formation. (B) Smp-KD and Gap27 exhibit synergistic effects on both segment length and regenerate length. Treatment with subthreshold doses of both Smp-MO and Gap27 lead to reduced segment length and regenerate length compared with either single treatment (one-way ANOVA with Tukey's multiple comparison as post-hoc test; *P<0.05). n=16; three independent replicates. (C) Sema3d-KD and Gap27 fail to exhibit synergistic effects for either segment length or regenerate length. Treatment with subthreshold doses of both Sema3d-MO and Gap27 are not significantly different from either single treatment (one-way ANOVA with Tukey's multiple comparison as post-hoc test; *P<0.05) (n=16, three independent replicates). Data are mean±s.e.m.

    Journal: Development (Cambridge, England)

    Article Title: Connexin 43 gap junctional intercellular communication inhibits evx1 expression and joint formation in regenerating fins

    doi: 10.1242/dev.190512

    Figure Lengend Snippet: Cx43-GJIC and Smp act in a common pathway. (A) Two previously described Cx43-dependent pathways impinging on joint formation. (B) Smp-KD and Gap27 exhibit synergistic effects on both segment length and regenerate length. Treatment with subthreshold doses of both Smp-MO and Gap27 lead to reduced segment length and regenerate length compared with either single treatment (one-way ANOVA with Tukey's multiple comparison as post-hoc test; *P<0.05). n=16; three independent replicates. (C) Sema3d-KD and Gap27 fail to exhibit synergistic effects for either segment length or regenerate length. Treatment with subthreshold doses of both Sema3d-MO and Gap27 are not significantly different from either single treatment (one-way ANOVA with Tukey's multiple comparison as post-hoc test; *P<0.05) (n=16, three independent replicates). Data are mean±s.e.m.

    Article Snippet: Peptide injections into regenerating fins Peptides used in this study include: Gap27 (Tocris, 1476), 5Fam-Gap27, scrambled Gap27 peptide and TAT-Gap19 (YGRKKRRQRRR-KQIEIKKFK, Tocris, 6227).

    Techniques:

    Cx43-GJIC influences changes in gene expression. (A) Proposed pathway for changes in gene expression influenced by Cx43-GJIC. (B) Gap27 injections lead to reduced smp expression compared with Scrm-Gap27 injections (n=15). Asterisks indicate identification of stained fin rays. (C) Gap27 injections lead to increased frequency of evx1 expression compared with Scrm-Gap27 injections (n=10, three independent replicates). + indicates fin rays positive for evx1; − indicates fin rays negative for evx1. (D) Bar graph showing increased percentage of evx1+ fin rays with Gap27 injections compared with Scrm-Gap27 injections (Student's t-test, two tailed and unpaired, *P<0.05). n=16; three independent replicates. Data are mean±s.e.m. Scale bar: 50 µm.

    Journal: Development (Cambridge, England)

    Article Title: Connexin 43 gap junctional intercellular communication inhibits evx1 expression and joint formation in regenerating fins

    doi: 10.1242/dev.190512

    Figure Lengend Snippet: Cx43-GJIC influences changes in gene expression. (A) Proposed pathway for changes in gene expression influenced by Cx43-GJIC. (B) Gap27 injections lead to reduced smp expression compared with Scrm-Gap27 injections (n=15). Asterisks indicate identification of stained fin rays. (C) Gap27 injections lead to increased frequency of evx1 expression compared with Scrm-Gap27 injections (n=10, three independent replicates). + indicates fin rays positive for evx1; − indicates fin rays negative for evx1. (D) Bar graph showing increased percentage of evx1+ fin rays with Gap27 injections compared with Scrm-Gap27 injections (Student's t-test, two tailed and unpaired, *P<0.05). n=16; three independent replicates. Data are mean±s.e.m. Scale bar: 50 µm.

    Article Snippet: Peptide injections into regenerating fins Peptides used in this study include: Gap27 (Tocris, 1476), 5Fam-Gap27, scrambled Gap27 peptide and TAT-Gap19 (YGRKKRRQRRR-KQIEIKKFK, Tocris, 6227).

    Techniques: Expressing, Staining, Two Tailed Test

    Cx43-GJIC influences active β-catenin levels. (A) Immunoblots showing reduced active β-catenin in Gap27-injected fins compared with Scrm-Gap27-injected fins. (B) Bar graph showing the average relative densities of the β-catenin bands between experimental and control samples from three biological replicates. The amount of active β-catenin is reduced by 39% in Gap27-injected lysates (Student's t-test, two tailed and unpaired, *P<0.05). Ten fins per lysate; three independent replicates. Data are mean±s.e.m.

    Journal: Development (Cambridge, England)

    Article Title: Connexin 43 gap junctional intercellular communication inhibits evx1 expression and joint formation in regenerating fins

    doi: 10.1242/dev.190512

    Figure Lengend Snippet: Cx43-GJIC influences active β-catenin levels. (A) Immunoblots showing reduced active β-catenin in Gap27-injected fins compared with Scrm-Gap27-injected fins. (B) Bar graph showing the average relative densities of the β-catenin bands between experimental and control samples from three biological replicates. The amount of active β-catenin is reduced by 39% in Gap27-injected lysates (Student's t-test, two tailed and unpaired, *P<0.05). Ten fins per lysate; three independent replicates. Data are mean±s.e.m.

    Article Snippet: Peptide injections into regenerating fins Peptides used in this study include: Gap27 (Tocris, 1476), 5Fam-Gap27, scrambled Gap27 peptide and TAT-Gap19 (YGRKKRRQRRR-KQIEIKKFK, Tocris, 6227).

    Techniques: Western Blot, Injection, Two Tailed Test

    Inhibition of Cx43-GJIC rescues cx43lh10 phenotypes. (A) Gap27 injection rescues regenerate length in cx43lh10 fins. Fins were amputated at the 50% level. The amputation plane is indicated (black line). Black arrows indicate the distance from the amputation plane to the distal tip of the third fin ray. Bar graph shows reduced regenerate length in cx43lh10 fins injected with Gap27 compared with cx43lh10 fins injected with Scrm-Gap27 (Student's t-test, two tailed and unpaired, *P<0.05). n=14; three independent replicates. (B) Gap27 injection rescues segment length in cx43lh10 fins. Representative images are shown. Arrows and arrowheads point to the first two joints following the amputation plane (white line). The inset shows a higher magnification of the segments. Bar graph shows reduced segment length in cx43lh10 fins injected with Gap27 compared with cx43lh10 fins injected with Scrm-Gap27. Student's t-test, two-tailed and unpaired, *P<0.05 (n=14, three independent replicates). Data are mean±s.e.m. Scale bar: 50 µm.

    Journal: Development (Cambridge, England)

    Article Title: Connexin 43 gap junctional intercellular communication inhibits evx1 expression and joint formation in regenerating fins

    doi: 10.1242/dev.190512

    Figure Lengend Snippet: Inhibition of Cx43-GJIC rescues cx43lh10 phenotypes. (A) Gap27 injection rescues regenerate length in cx43lh10 fins. Fins were amputated at the 50% level. The amputation plane is indicated (black line). Black arrows indicate the distance from the amputation plane to the distal tip of the third fin ray. Bar graph shows reduced regenerate length in cx43lh10 fins injected with Gap27 compared with cx43lh10 fins injected with Scrm-Gap27 (Student's t-test, two tailed and unpaired, *P<0.05). n=14; three independent replicates. (B) Gap27 injection rescues segment length in cx43lh10 fins. Representative images are shown. Arrows and arrowheads point to the first two joints following the amputation plane (white line). The inset shows a higher magnification of the segments. Bar graph shows reduced segment length in cx43lh10 fins injected with Gap27 compared with cx43lh10 fins injected with Scrm-Gap27. Student's t-test, two-tailed and unpaired, *P<0.05 (n=14, three independent replicates). Data are mean±s.e.m. Scale bar: 50 µm.

    Article Snippet: Peptide injections into regenerating fins Peptides used in this study include: Gap27 (Tocris, 1476), 5Fam-Gap27, scrambled Gap27 peptide and TAT-Gap19 (YGRKKRRQRRR-KQIEIKKFK, Tocris, 6227).

    Techniques: Inhibition, Injection, Two Tailed Test