14-878 Search Results


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  • 93
    ATCC nb atcc 14878
    Nb Atcc 14878, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nb atcc 14878/product/ATCC
    Average 93 stars, based on 1 article reviews
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    92
    Novus Biologicals rabbit monoclonal anti arpc5 antibody anti p16 arc
    Rabbit Monoclonal Anti Arpc5 Antibody Anti P16 Arc, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti arpc5 antibody anti p16 arc/product/Novus Biologicals
    Average 92 stars, based on 1 article reviews
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    93
    Proteintech anti vipr1
    Validation of the driver proteins' expression level in each group. (A) The band diagrams of 7 representative driver proteins and β-actin in each group. (B–H) The expression level comparison among groups for PAQR8, RhoA, EMC10, GGA2, <t>VIPR1,</t> and FAM120A, SEMA3F determined by western blot. * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, non-significant.
    Anti Vipr1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vipr1/product/Proteintech
    Average 93 stars, based on 1 article reviews
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    91
    Cayman Chemical pentostatin
    ( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or <t>pentostatin</t> (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.
    Pentostatin, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentostatin/product/Cayman Chemical
    Average 91 stars, based on 1 article reviews
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    86
    Merck KGaA photometric spectroquant toc cell test 14878
    ( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or <t>pentostatin</t> (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.
    Photometric Spectroquant Toc Cell Test 14878, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/photometric spectroquant toc cell test 14878/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    photometric spectroquant toc cell test 14878 - by Bioz Stars, 2024-02
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    Image Search Results


    Validation of the driver proteins' expression level in each group. (A) The band diagrams of 7 representative driver proteins and β-actin in each group. (B–H) The expression level comparison among groups for PAQR8, RhoA, EMC10, GGA2, VIPR1, and FAM120A, SEMA3F determined by western blot. * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, non-significant.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Modular Screening Reveals Driver Induced Additive Mechanisms of Baicalin and Jasminoidin on Cerebral Ischemia Therapy

    doi: 10.3389/fcvm.2022.813983

    Figure Lengend Snippet: Validation of the driver proteins' expression level in each group. (A) The band diagrams of 7 representative driver proteins and β-actin in each group. (B–H) The expression level comparison among groups for PAQR8, RhoA, EMC10, GGA2, VIPR1, and FAM120A, SEMA3F determined by western blot. * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, non-significant.

    Article Snippet: The membrane was then incubated with a secondary antibody, such as anti-RhoA (1:5,000 dilution, Abcam, ab187027), anti-EMC10 (1:500 dilution, Solarbio, K009317P), anti-SEMA3F (1:2,000 dilution, Affinity, DF8611), anti-VIPR1 (1:1,000 dilution, Affinity, DF5172), anti-PAQR8 (1:1,000 dilution, Novus, NBP2-92893), anti-GGA2 (1:1,000 dilution, Proteintech, 10356-1-AP), and β-actin (1:1,000 dilution, Boster, BM0627) as an internal control.

    Techniques: Expressing, Western Blot

    ( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or pentostatin (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.

    Journal: Science Advances

    Article Title: Cellular sensing of extracellular purine nucleosides triggers an innate IFN-β response

    doi: 10.1126/sciadv.aba3688

    Figure Lengend Snippet: ( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or pentostatin (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.

    Article Snippet: Pentostatin was purchased from Cayman Chemicals, cycloleucine was from MP Biomedicals, and 2′-deoxyinosine, inosine, and hypoxanthine were from Alfa Aeser.

    Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Activity Assay, Labeling, Transfection, Protein Concentration, Expressing