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    • streptococcus agalactiae  (ATCC)
    96
    ATCC streptococcus agalactiae
    Streptococcus Agalactiae, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptococcus agalactiae/product/ATCC
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptococcus agalactiae - by Bioz Stars, 2023-09
    96/100 stars
    		  Buy from Supplier
    sirt2 flag  (Addgene inc)
    93
    Addgene inc sirt2 flag
    ( A, B ) Initiation of the DDR, measured by co-localization of MRE11-LacR-Cherry (n = 136, p<0.005), SIRT6-LacR-GFP (n = 243, p<0.0001) or SIRT6 HY-LacR-GFP (n = 71, p<0.0001), compared to GFP-LacR (n = 310). Data are means for 4–9 experiments (error bars are SEMs). ( C, D ) Live imaging upon laser-induced damage (LID) of SIRT6-WT-GFP (n = 20) or SIRT6-HY-GFP (n = 20) in SIRT6 KO U2OS cells (n = 20). ( D ) Accumulation over time in 3 s intervals. ( E ) Average area under the curve of for three LID experiments (error bars are SEMs) (n[S6-WT]=40, n[S6-HY]=39, p<0.0001). ( F ) Initiation of DDR by full-length SIRT6-LacR-GFP (n = 127, p<0.005), Core-LacR-GFP (n = 66, p>0.05) or GFP-LacR (n = 64). Data are averages from 3–4 experiments (error bars show SEMs). ( G ) Initiation of DDR by SIRT6-LacR-GFP (n = 127, p<0.05), SIRT1-LacR-GFP (n = 44, p>0.05), <t>SIRT2-LacR-GFP</t> (n = 67, p<0.005), SIRT7-LacR-GFP (n = 68, p<0.005) or GFP-LacR (n = 64). Data are averages from 3–10 experiments (error bars show SEMs).
    Sirt2 Flag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirt2 flag/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sirt2 flag - by Bioz Stars, 2023-09
    93/100 stars
    		  Buy from Supplier
    axitinib  (Cayman Chemical)
    92
    Cayman Chemical axitinib
    ( A, B ) Initiation of the DDR, measured by co-localization of MRE11-LacR-Cherry (n = 136, p<0.005), SIRT6-LacR-GFP (n = 243, p<0.0001) or SIRT6 HY-LacR-GFP (n = 71, p<0.0001), compared to GFP-LacR (n = 310). Data are means for 4–9 experiments (error bars are SEMs). ( C, D ) Live imaging upon laser-induced damage (LID) of SIRT6-WT-GFP (n = 20) or SIRT6-HY-GFP (n = 20) in SIRT6 KO U2OS cells (n = 20). ( D ) Accumulation over time in 3 s intervals. ( E ) Average area under the curve of for three LID experiments (error bars are SEMs) (n[S6-WT]=40, n[S6-HY]=39, p<0.0001). ( F ) Initiation of DDR by full-length SIRT6-LacR-GFP (n = 127, p<0.005), Core-LacR-GFP (n = 66, p>0.05) or GFP-LacR (n = 64). Data are averages from 3–4 experiments (error bars show SEMs). ( G ) Initiation of DDR by SIRT6-LacR-GFP (n = 127, p<0.05), SIRT1-LacR-GFP (n = 44, p>0.05), <t>SIRT2-LacR-GFP</t> (n = 67, p<0.005), SIRT7-LacR-GFP (n = 68, p<0.005) or GFP-LacR (n = 64). Data are averages from 3–10 experiments (error bars show SEMs).
    Axitinib, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axitinib/product/Cayman Chemical
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    axitinib - by Bioz Stars, 2023-09
    92/100 stars
    		  Buy from Supplier
    agalactiae atcc 13813  (ATCC)
    86
    ATCC agalactiae atcc 13813
    ( A, B ) Initiation of the DDR, measured by co-localization of MRE11-LacR-Cherry (n = 136, p<0.005), SIRT6-LacR-GFP (n = 243, p<0.0001) or SIRT6 HY-LacR-GFP (n = 71, p<0.0001), compared to GFP-LacR (n = 310). Data are means for 4–9 experiments (error bars are SEMs). ( C, D ) Live imaging upon laser-induced damage (LID) of SIRT6-WT-GFP (n = 20) or SIRT6-HY-GFP (n = 20) in SIRT6 KO U2OS cells (n = 20). ( D ) Accumulation over time in 3 s intervals. ( E ) Average area under the curve of for three LID experiments (error bars are SEMs) (n[S6-WT]=40, n[S6-HY]=39, p<0.0001). ( F ) Initiation of DDR by full-length SIRT6-LacR-GFP (n = 127, p<0.005), Core-LacR-GFP (n = 66, p>0.05) or GFP-LacR (n = 64). Data are averages from 3–4 experiments (error bars show SEMs). ( G ) Initiation of DDR by SIRT6-LacR-GFP (n = 127, p<0.05), SIRT1-LacR-GFP (n = 44, p>0.05), <t>SIRT2-LacR-GFP</t> (n = 67, p<0.005), SIRT7-LacR-GFP (n = 68, p<0.005) or GFP-LacR (n = 64). Data are averages from 3–10 experiments (error bars show SEMs).
    Agalactiae Atcc 13813, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agalactiae atcc 13813/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agalactiae atcc 13813 - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier
    groel2 nr 13813  (BEI Resources)
    86
    BEI Resources groel2 nr 13813
    ( A, B ) Initiation of the DDR, measured by co-localization of MRE11-LacR-Cherry (n = 136, p<0.005), SIRT6-LacR-GFP (n = 243, p<0.0001) or SIRT6 HY-LacR-GFP (n = 71, p<0.0001), compared to GFP-LacR (n = 310). Data are means for 4–9 experiments (error bars are SEMs). ( C, D ) Live imaging upon laser-induced damage (LID) of SIRT6-WT-GFP (n = 20) or SIRT6-HY-GFP (n = 20) in SIRT6 KO U2OS cells (n = 20). ( D ) Accumulation over time in 3 s intervals. ( E ) Average area under the curve of for three LID experiments (error bars are SEMs) (n[S6-WT]=40, n[S6-HY]=39, p<0.0001). ( F ) Initiation of DDR by full-length SIRT6-LacR-GFP (n = 127, p<0.005), Core-LacR-GFP (n = 66, p>0.05) or GFP-LacR (n = 64). Data are averages from 3–4 experiments (error bars show SEMs). ( G ) Initiation of DDR by SIRT6-LacR-GFP (n = 127, p<0.05), SIRT1-LacR-GFP (n = 44, p>0.05), <t>SIRT2-LacR-GFP</t> (n = 67, p<0.005), SIRT7-LacR-GFP (n = 68, p<0.005) or GFP-LacR (n = 64). Data are averages from 3–10 experiments (error bars show SEMs).
    Groel2 Nr 13813, supplied by BEI Resources, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/groel2 nr 13813/product/BEI Resources
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    groel2 nr 13813 - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A, B ) Initiation of the DDR, measured by co-localization of MRE11-LacR-Cherry (n = 136, p<0.005), SIRT6-LacR-GFP (n = 243, p<0.0001) or SIRT6 HY-LacR-GFP (n = 71, p<0.0001), compared to GFP-LacR (n = 310). Data are means for 4–9 experiments (error bars are SEMs). ( C, D ) Live imaging upon laser-induced damage (LID) of SIRT6-WT-GFP (n = 20) or SIRT6-HY-GFP (n = 20) in SIRT6 KO U2OS cells (n = 20). ( D ) Accumulation over time in 3 s intervals. ( E ) Average area under the curve of for three LID experiments (error bars are SEMs) (n[S6-WT]=40, n[S6-HY]=39, p<0.0001). ( F ) Initiation of DDR by full-length SIRT6-LacR-GFP (n = 127, p<0.005), Core-LacR-GFP (n = 66, p>0.05) or GFP-LacR (n = 64). Data are averages from 3–4 experiments (error bars show SEMs). ( G ) Initiation of DDR by SIRT6-LacR-GFP (n = 127, p<0.05), SIRT1-LacR-GFP (n = 44, p>0.05), SIRT2-LacR-GFP (n = 67, p<0.005), SIRT7-LacR-GFP (n = 68, p<0.005) or GFP-LacR (n = 64). Data are averages from 3–10 experiments (error bars show SEMs).

    Journal: eLife

    Article Title: SIRT6 is a DNA double-strand break sensor

    doi: 10.7554/eLife.51636

    Figure Lengend Snippet: ( A, B ) Initiation of the DDR, measured by co-localization of MRE11-LacR-Cherry (n = 136, p<0.005), SIRT6-LacR-GFP (n = 243, p<0.0001) or SIRT6 HY-LacR-GFP (n = 71, p<0.0001), compared to GFP-LacR (n = 310). Data are means for 4–9 experiments (error bars are SEMs). ( C, D ) Live imaging upon laser-induced damage (LID) of SIRT6-WT-GFP (n = 20) or SIRT6-HY-GFP (n = 20) in SIRT6 KO U2OS cells (n = 20). ( D ) Accumulation over time in 3 s intervals. ( E ) Average area under the curve of for three LID experiments (error bars are SEMs) (n[S6-WT]=40, n[S6-HY]=39, p<0.0001). ( F ) Initiation of DDR by full-length SIRT6-LacR-GFP (n = 127, p<0.005), Core-LacR-GFP (n = 66, p>0.05) or GFP-LacR (n = 64). Data are averages from 3–4 experiments (error bars show SEMs). ( G ) Initiation of DDR by SIRT6-LacR-GFP (n = 127, p<0.05), SIRT1-LacR-GFP (n = 44, p>0.05), SIRT2-LacR-GFP (n = 67, p<0.005), SIRT7-LacR-GFP (n = 68, p<0.005) or GFP-LacR (n = 64). Data are averages from 3–10 experiments (error bars show SEMs).

    Article Snippet: Recombinant DNA reagent , SIRT2-Flag , Addgene , 13813 , .

    Techniques: Imaging

    ( A ) Western blot of nuclear protein fraction of LacO containing U2OS cells after 12 or 24 hr treatment with 0, 5 or 10 mM nicotinamide (NAM) added to their media. ( B ) Co-localization percentage with γH2AX of SIRT6 HY-LacR-GFP and GFP-LacR after 24 hr of treatment with NAM at three concentrations (*, p<0.05; **, p<0.005; ***, p<0.0005). ( C ) Immunofluorescence (IF) of co-localization of Core-LacR-GFP (n = 66, p>0.05). Experiment quantification appears in . ( D ) IF of co-localization of SIRT1-LacR-GFP (n = 44, p>0.05), SIRT2-LacR-GFP (n = 67, p<0.005) and SIRT7-LacR-GFP (n = 68, p<0.005). Experiment quantification appears in .

    Journal: eLife

    Article Title: SIRT6 is a DNA double-strand break sensor

    doi: 10.7554/eLife.51636

    Figure Lengend Snippet: ( A ) Western blot of nuclear protein fraction of LacO containing U2OS cells after 12 or 24 hr treatment with 0, 5 or 10 mM nicotinamide (NAM) added to their media. ( B ) Co-localization percentage with γH2AX of SIRT6 HY-LacR-GFP and GFP-LacR after 24 hr of treatment with NAM at three concentrations (*, p<0.05; **, p<0.005; ***, p<0.0005). ( C ) Immunofluorescence (IF) of co-localization of Core-LacR-GFP (n = 66, p>0.05). Experiment quantification appears in . ( D ) IF of co-localization of SIRT1-LacR-GFP (n = 44, p>0.05), SIRT2-LacR-GFP (n = 67, p<0.005) and SIRT7-LacR-GFP (n = 68, p<0.005). Experiment quantification appears in .

    Article Snippet: Recombinant DNA reagent , SIRT2-Flag , Addgene , 13813 , .

    Techniques: Western Blot, Immunofluorescence

    Journal: eLife

    Article Title: SIRT6 is a DNA double-strand break sensor

    doi: 10.7554/eLife.51636

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , SIRT2-Flag , Addgene , 13813 , .

    Techniques: Recombinant, DNA Binding Assay