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  • 93
    Proteintech t bet
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    Genes with significantly enhanced mRNA signal in CD4γ13 T cells
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    Image Search Results


    Genes with significantly enhanced mRNA signal in CD4γ13 T cells

    Journal: Infection and Immunity

    Article Title: B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters

    doi: 10.1128/IAI.00614-17

    Figure Lengend Snippet: Genes with significantly enhanced mRNA signal in CD4γ13 T cells

    Article Snippet: Polyclonal rabbit antiserum specific for Eomes (Thermo Fisher Scientific catalog no. 720202), Tbet / Gata3 / Fhl2 (Proteintech catalog no. 13700-1-AP/10417-1-AP/21619-1-AP), and HRP-coupled monoclonal antibody to β-actin (Sigma-Aldrich catalog no. A3854) were obtained from commercial vendors.

    Techniques: Cell Differentiation, Migration, Knock-Out, Modification, Binding Assay, Activation Assay

    Expression of CD4γ13 differentiation markers and transcription factors

    Journal: Infection and Immunity

    Article Title: B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters

    doi: 10.1128/IAI.00614-17

    Figure Lengend Snippet: Expression of CD4γ13 differentiation markers and transcription factors

    Article Snippet: Polyclonal rabbit antiserum specific for Eomes (Thermo Fisher Scientific catalog no. 720202), Tbet / Gata3 / Fhl2 (Proteintech catalog no. 13700-1-AP/10417-1-AP/21619-1-AP), and HRP-coupled monoclonal antibody to β-actin (Sigma-Aldrich catalog no. A3854) were obtained from commercial vendors.

    Techniques: Expressing, Binding Assay

    Protein levels of selected differentiation/transcription factors. On day 5 of the usual culture cycle, the indicated CD4 T cell clones were purified by Ficoll-Hypaque, and whole-cell lysates were prepared and utilized for immunoblotting for Tbet, Gata3, Eomes, and Fhl2. Blots were stripped and reprobed with anti-β-actin as the loading control. Molecular mass markers (M) in kilodaltons are indicated in the right margin.

    Journal: Infection and Immunity

    Article Title: B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters

    doi: 10.1128/IAI.00614-17

    Figure Lengend Snippet: Protein levels of selected differentiation/transcription factors. On day 5 of the usual culture cycle, the indicated CD4 T cell clones were purified by Ficoll-Hypaque, and whole-cell lysates were prepared and utilized for immunoblotting for Tbet, Gata3, Eomes, and Fhl2. Blots were stripped and reprobed with anti-β-actin as the loading control. Molecular mass markers (M) in kilodaltons are indicated in the right margin.

    Article Snippet: Polyclonal rabbit antiserum specific for Eomes (Thermo Fisher Scientific catalog no. 720202), Tbet / Gata3 / Fhl2 (Proteintech catalog no. 13700-1-AP/10417-1-AP/21619-1-AP), and HRP-coupled monoclonal antibody to β-actin (Sigma-Aldrich catalog no. A3854) were obtained from commercial vendors.

    Techniques: Clone Assay, Purification, Western Blot