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    Phosphorylation of exercise‐related signaling proteins and dephosphorylation of <t> CRTC2 </t> in exercised m. vastus lateralis prior to and at 2 min after, the one‐legged continuous knee extension exercise
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    Phosphorylation of exercise‐related signaling proteins and dephosphorylation of <t> CRTC2 </t> in exercised m. vastus lateralis prior to and at 2 min after, the one‐legged continuous knee extension exercise
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    Phosphorylation of exercise‐related signaling proteins and dephosphorylation of <t> CRTC2 </t> in exercised m. vastus lateralis prior to and at 2 min after, the one‐legged continuous knee extension exercise
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    Phosphorylation of exercise‐related signaling proteins and dephosphorylation of <t> CRTC2 </t> in exercised m. vastus lateralis prior to and at 2 min after, the one‐legged continuous knee extension exercise
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    Phosphorylation of exercise‐related signaling proteins and dephosphorylation of  CRTC2  in exercised m. vastus lateralis prior to and at 2 min after, the one‐legged continuous knee extension exercise

    Journal: Physiological Reports

    Article Title: Regulation of PPARGC1A gene expression in trained and untrained human skeletal muscle

    doi: 10.14814/phy2.13543

    Figure Lengend Snippet: Phosphorylation of exercise‐related signaling proteins and dephosphorylation of CRTC2 in exercised m. vastus lateralis prior to and at 2 min after, the one‐legged continuous knee extension exercise

    Article Snippet: The proteins were transferred to nitrocellulose membranes using a Trans‐Blot Turbo system (Bio‐Rad, USA) in Towbin buffer for 30 min at 25 V. The membranes were stained with Ponceau S to verify consistent loading of proteins, followed by washing and incubation in 5% nonfat dry milk for 1 h. The membranes were subsequently incubated at 4°C overnight with antibodies specific for phospho‐acetyl‐CoA carboxylase (ACC Ser79/222 ; 1:1000; ab68191; Abcam, UK), phospho‐mitogen‐activated protein kinase (p38 Thr180/Tyr182 ; 1:500; ab195049; Abcam), phospho‐Ca 2+ /calmodulin‐dependent protein kinase II (CaMKII Thr286 ; 1:2500; ab32678; Abcam), phospho‐CREB1 Ser133 (1:500; ab32096; Abcam), CREB‐regulated transcription coactivator 2 (CRTC2; 1:500, #13017; Cell Signaling), and oxidative phosphorylation proteins (OXPHOS): NDUFB8, SDHB, UQCRC2, MT‐CO1, and ATP5A1 (1:2500; ab110413; Abcam) followed by incubation for 1 h at room temperature with an anti‐rabbit secondary antibody (Cell Signaling).

    Techniques: De-Phosphorylation Assay