1248 tyr p erbb2 Search Results


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  • erbb2  (Abcam)
    86
    Abcam erbb2
    Increased NRG-1 and activation of <t>ErbB2/4</t> by both IP and NRG-1 in vivo. ( a ), Representative protein levels of NRG-1. ( b ), Semi-quantification of protein levels of NRG-1. ( c ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( d ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( e ), Representative protein levels of P-ErbB2 and T-ErbB2. ( f ), Semi-quantification of protein levels of P-ErbB2/T-ErbB2.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05 vs. CON, * p < 0.05 vs. IR
    Erbb2, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erbb2/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    erbb2 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Millipore appropriate horseradish peroxidase
    Increased NRG-1 and activation of <t>ErbB2/4</t> by both IP and NRG-1 in vivo. ( a ), Representative protein levels of NRG-1. ( b ), Semi-quantification of protein levels of NRG-1. ( c ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( d ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( e ), Representative protein levels of P-ErbB2 and T-ErbB2. ( f ), Semi-quantification of protein levels of P-ErbB2/T-ErbB2.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05 vs. CON, * p < 0.05 vs. IR
    Appropriate Horseradish Peroxidase, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/appropriate horseradish peroxidase/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    appropriate horseradish peroxidase - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Abcam appropriate horseradish peroxidase
    Increased NRG-1 and activation of <t>ErbB2/4</t> by both IP and NRG-1 in vivo. ( a ), Representative protein levels of NRG-1. ( b ), Semi-quantification of protein levels of NRG-1. ( c ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( d ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( e ), Representative protein levels of P-ErbB2 and T-ErbB2. ( f ), Semi-quantification of protein levels of P-ErbB2/T-ErbB2.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05 vs. CON, * p < 0.05 vs. IR
    Appropriate Horseradish Peroxidase, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/appropriate horseradish peroxidase/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    appropriate horseradish peroxidase - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc 473 ser p akt
    Increased NRG-1 and activation of <t>ErbB2/4</t> by both IP and NRG-1 in vivo. ( a ), Representative protein levels of NRG-1. ( b ), Semi-quantification of protein levels of NRG-1. ( c ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( d ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( e ), Representative protein levels of P-ErbB2 and T-ErbB2. ( f ), Semi-quantification of protein levels of P-ErbB2/T-ErbB2.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05 vs. CON, * p < 0.05 vs. IR
    473 Ser P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/473 ser p akt/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    473 ser p akt - by Bioz Stars, 2024-06
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    nrg 1  (Abcam)
    86
    Abcam nrg 1
    Protective effects of both IP and <t>NRG-1</t> by reducing the IS and apoptosis induced by IR in vivo . ( a ), Representative heart slices stained by Evans blue and TTC. Blue: non-ischaemic area; non-blue: the area at risk (AR); white: infarct size (IS). ( b ), The percentage of infarct size/area at risk (IS/AR%). ( c ), Representative myocardial apoptosis in paraffin sections of the heart at the risk area. The normal cellular nuclei were stained blue by haematoxylin; the apoptotic nuclei were stained brown by TUNEL assay. ( d ), The percentage of TUNEL-positive cells in the total cells. ( e ), Representative protein levels of pro-caspase 3 and cleaved-caspase 3 by western blotting. ( f ), Semi-quantification of cleaved-caspase 3 protein levels normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05, ### p < 0.001 vs. CON, *p < 0.05, ** p < 0.01 vs. IR
    Nrg 1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nrg 1/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nrg 1 - by Bioz Stars, 2024-06
    86/100 stars
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    Image Search Results


    Increased NRG-1 and activation of ErbB2/4 by both IP and NRG-1 in vivo. ( a ), Representative protein levels of NRG-1. ( b ), Semi-quantification of protein levels of NRG-1. ( c ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( d ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( e ), Representative protein levels of P-ErbB2 and T-ErbB2. ( f ), Semi-quantification of protein levels of P-ErbB2/T-ErbB2.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05 vs. CON, * p < 0.05 vs. IR

    Journal: Molecular Medicine

    Article Title: Pharmacological postconditioning with Neuregulin-1 mimics the cardioprotective effects of ischaemic postconditioning via ErbB4-dependent activation of reperfusion injury salvage kinase pathway

    doi: 10.1186/s10020-018-0040-7

    Figure Lengend Snippet: Increased NRG-1 and activation of ErbB2/4 by both IP and NRG-1 in vivo. ( a ), Representative protein levels of NRG-1. ( b ), Semi-quantification of protein levels of NRG-1. ( c ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( d ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( e ), Representative protein levels of P-ErbB2 and T-ErbB2. ( f ), Semi-quantification of protein levels of P-ErbB2/T-ErbB2.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05 vs. CON, * p < 0.05 vs. IR

    Article Snippet: Primary antibodies used in the experiment were as follows: 202 Thr/ 204 Tyr-P-ERK1/2, T-ERK1/2, 473 Ser-P-AKT, T-AKT (pan), 389 Thr-P-p70S6k, p70S6k, 172 Thr-P-AMPK, AMPK (rabbit monoclonal antibodies, Cell Signaling Technology, USA) and caspase 3 antibody (rabbit polyclonal antibody, Cell Signaling Technology, USA); 1248 Tyr-P-ErbB2, ErbB2, 1284 Tyr-P-ErbB4, T-ErbB4, and NRG-1 (rabbit polyclonal antibodies, Abcam, USA); GAPDH (mouse anti-human monoclonal antibody, Millipore, USA) and appropriate horseradish peroxidase-conjugated secondary antibody (ZSGB-BIO, China).

    Techniques: Activation Assay, In Vivo, Western Blot

    Protective effects of both IP and NRG-1 by reducing the IS and apoptosis induced by IR in vivo . ( a ), Representative heart slices stained by Evans blue and TTC. Blue: non-ischaemic area; non-blue: the area at risk (AR); white: infarct size (IS). ( b ), The percentage of infarct size/area at risk (IS/AR%). ( c ), Representative myocardial apoptosis in paraffin sections of the heart at the risk area. The normal cellular nuclei were stained blue by haematoxylin; the apoptotic nuclei were stained brown by TUNEL assay. ( d ), The percentage of TUNEL-positive cells in the total cells. ( e ), Representative protein levels of pro-caspase 3 and cleaved-caspase 3 by western blotting. ( f ), Semi-quantification of cleaved-caspase 3 protein levels normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05, ### p < 0.001 vs. CON, *p < 0.05, ** p < 0.01 vs. IR

    Journal: Molecular Medicine

    Article Title: Pharmacological postconditioning with Neuregulin-1 mimics the cardioprotective effects of ischaemic postconditioning via ErbB4-dependent activation of reperfusion injury salvage kinase pathway

    doi: 10.1186/s10020-018-0040-7

    Figure Lengend Snippet: Protective effects of both IP and NRG-1 by reducing the IS and apoptosis induced by IR in vivo . ( a ), Representative heart slices stained by Evans blue and TTC. Blue: non-ischaemic area; non-blue: the area at risk (AR); white: infarct size (IS). ( b ), The percentage of infarct size/area at risk (IS/AR%). ( c ), Representative myocardial apoptosis in paraffin sections of the heart at the risk area. The normal cellular nuclei were stained blue by haematoxylin; the apoptotic nuclei were stained brown by TUNEL assay. ( d ), The percentage of TUNEL-positive cells in the total cells. ( e ), Representative protein levels of pro-caspase 3 and cleaved-caspase 3 by western blotting. ( f ), Semi-quantification of cleaved-caspase 3 protein levels normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05, ### p < 0.001 vs. CON, *p < 0.05, ** p < 0.01 vs. IR

    Article Snippet: Primary antibodies used in the experiment were as follows: 202 Thr/ 204 Tyr-P-ERK1/2, T-ERK1/2, 473 Ser-P-AKT, T-AKT (pan), 389 Thr-P-p70S6k, p70S6k, 172 Thr-P-AMPK, AMPK (rabbit monoclonal antibodies, Cell Signaling Technology, USA) and caspase 3 antibody (rabbit polyclonal antibody, Cell Signaling Technology, USA); 1248 Tyr-P-ErbB2, ErbB2, 1284 Tyr-P-ErbB4, T-ErbB4, and NRG-1 (rabbit polyclonal antibodies, Abcam, USA); GAPDH (mouse anti-human monoclonal antibody, Millipore, USA) and appropriate horseradish peroxidase-conjugated secondary antibody (ZSGB-BIO, China).

    Techniques: In Vivo, Staining, TUNEL Assay, Western Blot

    Increased NRG-1 and activation of ErbB2/4 by both IP and NRG-1 in vivo. ( a ), Representative protein levels of NRG-1. ( b ), Semi-quantification of protein levels of NRG-1. ( c ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( d ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( e ), Representative protein levels of P-ErbB2 and T-ErbB2. ( f ), Semi-quantification of protein levels of P-ErbB2/T-ErbB2.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05 vs. CON, * p < 0.05 vs. IR

    Journal: Molecular Medicine

    Article Title: Pharmacological postconditioning with Neuregulin-1 mimics the cardioprotective effects of ischaemic postconditioning via ErbB4-dependent activation of reperfusion injury salvage kinase pathway

    doi: 10.1186/s10020-018-0040-7

    Figure Lengend Snippet: Increased NRG-1 and activation of ErbB2/4 by both IP and NRG-1 in vivo. ( a ), Representative protein levels of NRG-1. ( b ), Semi-quantification of protein levels of NRG-1. ( c ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( d ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( e ), Representative protein levels of P-ErbB2 and T-ErbB2. ( f ), Semi-quantification of protein levels of P-ErbB2/T-ErbB2.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). # p < 0.05 vs. CON, * p < 0.05 vs. IR

    Article Snippet: Primary antibodies used in the experiment were as follows: 202 Thr/ 204 Tyr-P-ERK1/2, T-ERK1/2, 473 Ser-P-AKT, T-AKT (pan), 389 Thr-P-p70S6k, p70S6k, 172 Thr-P-AMPK, AMPK (rabbit monoclonal antibodies, Cell Signaling Technology, USA) and caspase 3 antibody (rabbit polyclonal antibody, Cell Signaling Technology, USA); 1248 Tyr-P-ErbB2, ErbB2, 1284 Tyr-P-ErbB4, T-ErbB4, and NRG-1 (rabbit polyclonal antibodies, Abcam, USA); GAPDH (mouse anti-human monoclonal antibody, Millipore, USA) and appropriate horseradish peroxidase-conjugated secondary antibody (ZSGB-BIO, China).

    Techniques: Activation Assay, In Vivo, Western Blot

    Protective effects of IP and NRG-1 were suppressed by the ErbB4 inhibitor AG1478 ex vivo . ( a ), Representative heart slices stained by TTC, red: the ischaemic area, white: infarct area. ( b ), the percentage of infarct size/left ventricle (IS/LV%). ( c ), Histological analysis of heart sections in Langendorff model by hematoxylin and eosin staining. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). * p < 0.05 vs. IR; # p < 0.05 vs. the same treated group without AG1478

    Journal: Molecular Medicine

    Article Title: Pharmacological postconditioning with Neuregulin-1 mimics the cardioprotective effects of ischaemic postconditioning via ErbB4-dependent activation of reperfusion injury salvage kinase pathway

    doi: 10.1186/s10020-018-0040-7

    Figure Lengend Snippet: Protective effects of IP and NRG-1 were suppressed by the ErbB4 inhibitor AG1478 ex vivo . ( a ), Representative heart slices stained by TTC, red: the ischaemic area, white: infarct area. ( b ), the percentage of infarct size/left ventricle (IS/LV%). ( c ), Histological analysis of heart sections in Langendorff model by hematoxylin and eosin staining. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). * p < 0.05 vs. IR; # p < 0.05 vs. the same treated group without AG1478

    Article Snippet: Primary antibodies used in the experiment were as follows: 202 Thr/ 204 Tyr-P-ERK1/2, T-ERK1/2, 473 Ser-P-AKT, T-AKT (pan), 389 Thr-P-p70S6k, p70S6k, 172 Thr-P-AMPK, AMPK (rabbit monoclonal antibodies, Cell Signaling Technology, USA) and caspase 3 antibody (rabbit polyclonal antibody, Cell Signaling Technology, USA); 1248 Tyr-P-ErbB2, ErbB2, 1284 Tyr-P-ErbB4, T-ErbB4, and NRG-1 (rabbit polyclonal antibodies, Abcam, USA); GAPDH (mouse anti-human monoclonal antibody, Millipore, USA) and appropriate horseradish peroxidase-conjugated secondary antibody (ZSGB-BIO, China).

    Techniques: Ex Vivo, Staining

    Activation of the RISK pathway by IP and NRG-1 in vivo. ( a ), Representative protein levels of P-ERK1/2 and T-ERK1/2 by western blotting. ( b ), Semi-quantification of the density ratio of P-ERK1/2/T-ERK1/2. ( c ), Representative protein levels of P-AKT and T-AKT by western blotting. ( d ), Semi-quantification of the density ratio of P-AKT/T-AKT. ( e ), Representative protein levels of P-AMPK and T-AMPK by western blotting. ( f ), Semi-quantification of the density ratio of P-AMPK/T-AMPK. These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM (n = 6). # p < 0.05 vs. CON; * p < 0.05, ** p < 0.01 vs. IR

    Journal: Molecular Medicine

    Article Title: Pharmacological postconditioning with Neuregulin-1 mimics the cardioprotective effects of ischaemic postconditioning via ErbB4-dependent activation of reperfusion injury salvage kinase pathway

    doi: 10.1186/s10020-018-0040-7

    Figure Lengend Snippet: Activation of the RISK pathway by IP and NRG-1 in vivo. ( a ), Representative protein levels of P-ERK1/2 and T-ERK1/2 by western blotting. ( b ), Semi-quantification of the density ratio of P-ERK1/2/T-ERK1/2. ( c ), Representative protein levels of P-AKT and T-AKT by western blotting. ( d ), Semi-quantification of the density ratio of P-AKT/T-AKT. ( e ), Representative protein levels of P-AMPK and T-AMPK by western blotting. ( f ), Semi-quantification of the density ratio of P-AMPK/T-AMPK. These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM (n = 6). # p < 0.05 vs. CON; * p < 0.05, ** p < 0.01 vs. IR

    Article Snippet: Primary antibodies used in the experiment were as follows: 202 Thr/ 204 Tyr-P-ERK1/2, T-ERK1/2, 473 Ser-P-AKT, T-AKT (pan), 389 Thr-P-p70S6k, p70S6k, 172 Thr-P-AMPK, AMPK (rabbit monoclonal antibodies, Cell Signaling Technology, USA) and caspase 3 antibody (rabbit polyclonal antibody, Cell Signaling Technology, USA); 1248 Tyr-P-ErbB2, ErbB2, 1284 Tyr-P-ErbB4, T-ErbB4, and NRG-1 (rabbit polyclonal antibodies, Abcam, USA); GAPDH (mouse anti-human monoclonal antibody, Millipore, USA) and appropriate horseradish peroxidase-conjugated secondary antibody (ZSGB-BIO, China).

    Techniques: Activation Assay, In Vivo, Western Blot

    The phosphorylation of ErbB4 and the RISK pathway inhibited by AG1478 ex vivo. ( a ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( b ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( c ), Representative protein levels of P-ERK1/2 and T-ERK1/2 by western blotting. ( d ), Semi-quantification of the density ratio of P-ERK1/2/T-ERK1/2. ( e ), Representative protein levels of P-AKT and T-AKT by western blotting. ( f ), Semi-quantification of the density ratio of P-AKT/T-AKT. These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. IR; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the same treated group without AG1478

    Journal: Molecular Medicine

    Article Title: Pharmacological postconditioning with Neuregulin-1 mimics the cardioprotective effects of ischaemic postconditioning via ErbB4-dependent activation of reperfusion injury salvage kinase pathway

    doi: 10.1186/s10020-018-0040-7

    Figure Lengend Snippet: The phosphorylation of ErbB4 and the RISK pathway inhibited by AG1478 ex vivo. ( a ), Representative protein levels of P-ErbB4 and T-ErbB4 by western blotting. ( b ), Semi-quantification of the density ratio of P-ErbB4/T-ErbB4. ( c ), Representative protein levels of P-ERK1/2 and T-ERK1/2 by western blotting. ( d ), Semi-quantification of the density ratio of P-ERK1/2/T-ERK1/2. ( e ), Representative protein levels of P-AKT and T-AKT by western blotting. ( f ), Semi-quantification of the density ratio of P-AKT/T-AKT. These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM ( n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. IR; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the same treated group without AG1478

    Article Snippet: Primary antibodies used in the experiment were as follows: 202 Thr/ 204 Tyr-P-ERK1/2, T-ERK1/2, 473 Ser-P-AKT, T-AKT (pan), 389 Thr-P-p70S6k, p70S6k, 172 Thr-P-AMPK, AMPK (rabbit monoclonal antibodies, Cell Signaling Technology, USA) and caspase 3 antibody (rabbit polyclonal antibody, Cell Signaling Technology, USA); 1248 Tyr-P-ErbB2, ErbB2, 1284 Tyr-P-ErbB4, T-ErbB4, and NRG-1 (rabbit polyclonal antibodies, Abcam, USA); GAPDH (mouse anti-human monoclonal antibody, Millipore, USA) and appropriate horseradish peroxidase-conjugated secondary antibody (ZSGB-BIO, China).

    Techniques: Ex Vivo, Western Blot

    Protective effects of IP and NRG-1 abolished by PD980509 and LY294002 ex vivo. ( a ), Representative heart slices stained by TTC, red: the ischaemic area, white: infarct area. ( b ), The percentage of infarct size/left ventricle (IS/LV%). ( c ), Representative protein levels of P-ERK1/2 and T-ERK1/2 by western blotting. ( d ), Semi-quantification of the density ratio of P-ERK1/2/T-ERK1/2. ( e ), Representative protein levels of P-AKT and T-AKT by western blotting. ( f ), Semi-quantification of the density ratio of P-AKT/T-AKT. ( g ), Representative protein levels of P-p70s6k and T-p70s6k by western blotting. ( h ), Semi-quantification of the density ratio of P-p70s6k/T-p70s6k.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1, PD: PD98059, LY: LY294002. Data are shown as the mean ± SEM ( n = 6). * p < 0.05, ** p < 0.01 vs. IR; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the same treated group without inhibitor

    Journal: Molecular Medicine

    Article Title: Pharmacological postconditioning with Neuregulin-1 mimics the cardioprotective effects of ischaemic postconditioning via ErbB4-dependent activation of reperfusion injury salvage kinase pathway

    doi: 10.1186/s10020-018-0040-7

    Figure Lengend Snippet: Protective effects of IP and NRG-1 abolished by PD980509 and LY294002 ex vivo. ( a ), Representative heart slices stained by TTC, red: the ischaemic area, white: infarct area. ( b ), The percentage of infarct size/left ventricle (IS/LV%). ( c ), Representative protein levels of P-ERK1/2 and T-ERK1/2 by western blotting. ( d ), Semi-quantification of the density ratio of P-ERK1/2/T-ERK1/2. ( e ), Representative protein levels of P-AKT and T-AKT by western blotting. ( f ), Semi-quantification of the density ratio of P-AKT/T-AKT. ( g ), Representative protein levels of P-p70s6k and T-p70s6k by western blotting. ( h ), Semi-quantification of the density ratio of P-p70s6k/T-p70s6k.These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1, PD: PD98059, LY: LY294002. Data are shown as the mean ± SEM ( n = 6). * p < 0.05, ** p < 0.01 vs. IR; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the same treated group without inhibitor

    Article Snippet: Primary antibodies used in the experiment were as follows: 202 Thr/ 204 Tyr-P-ERK1/2, T-ERK1/2, 473 Ser-P-AKT, T-AKT (pan), 389 Thr-P-p70S6k, p70S6k, 172 Thr-P-AMPK, AMPK (rabbit monoclonal antibodies, Cell Signaling Technology, USA) and caspase 3 antibody (rabbit polyclonal antibody, Cell Signaling Technology, USA); 1248 Tyr-P-ErbB2, ErbB2, 1284 Tyr-P-ErbB4, T-ErbB4, and NRG-1 (rabbit polyclonal antibodies, Abcam, USA); GAPDH (mouse anti-human monoclonal antibody, Millipore, USA) and appropriate horseradish peroxidase-conjugated secondary antibody (ZSGB-BIO, China).

    Techniques: Ex Vivo, Staining, Western Blot