12237 Search Results


s canus atcc 12237 kun68828c  (ATCC)
91
ATCC s canus atcc 12237 kun68828c
S Canus Atcc 12237 Kun68828c, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s canus atcc 12237 kun68828c/product/ATCC
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
s canus atcc 12237 kun68828c - by Bioz Stars, 2024-10
91/100 stars
  Buy from Supplier
laforin reactions  (Chem Impex International)
95
Chem Impex International laforin reactions
Effect of treating 32P-labeled glycogen with <t>laforin</t> or glucosidases. A Glycogen was labeled by incubation with 5 <t>μM</t> <t>[β-32P]UDP-glucose</t> (lower panel) or [U-14C]glucose (upper panel) and yeast glycogen synthase (2 μg/ml) for 30 min. Glycogen was precipitated with ethanol, dissolved in buffer and treated with α-glucosidases (α-amylase and amyloglucosidase) (α-G), inactive mutant laforin (C266S Laf) or wild type laforin (WT Laf) as indicated, and analyzed by SDS-Page (see Materials and Methods). Dried gels were analyzed by a Phosphorimager. C, control reaction lacking glycogen synthase; NT, not treated. B UDP-glucose and UDP were incubated with active (WT) or inactive (C266S) laforin as indicated and analyzed by HPAEC. Chromatograms of UDP, UMP and UDP-glucose standards are shown in the lowermost panel.
Laforin Reactions, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/laforin reactions/product/Chem Impex International
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
laforin reactions - by Bioz Stars, 2024-10
95/100 stars
  Buy from Supplier
triticum urartu triur3 12237 triur3 12237 t1  (Medicago)
86
Medicago triticum urartu triur3 12237 triur3 12237 t1
Effect of treating 32P-labeled glycogen with <t>laforin</t> or glucosidases. A Glycogen was labeled by incubation with 5 <t>μM</t> <t>[β-32P]UDP-glucose</t> (lower panel) or [U-14C]glucose (upper panel) and yeast glycogen synthase (2 μg/ml) for 30 min. Glycogen was precipitated with ethanol, dissolved in buffer and treated with α-glucosidases (α-amylase and amyloglucosidase) (α-G), inactive mutant laforin (C266S Laf) or wild type laforin (WT Laf) as indicated, and analyzed by SDS-Page (see Materials and Methods). Dried gels were analyzed by a Phosphorimager. C, control reaction lacking glycogen synthase; NT, not treated. B UDP-glucose and UDP were incubated with active (WT) or inactive (C266S) laforin as indicated and analyzed by HPAEC. Chromatograms of UDP, UMP and UDP-glucose standards are shown in the lowermost panel.
Triticum Urartu Triur3 12237 Triur3 12237 T1, supplied by Medicago, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triticum urartu triur3 12237 triur3 12237 t1/product/Medicago
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triticum urartu triur3 12237 triur3 12237 t1 - by Bioz Stars, 2024-10
86/100 stars
  Buy from Supplier
expression plasmid pcdna3 12237  (Thermo Fisher)
86
Thermo Fisher expression plasmid pcdna3 12237
Effect of treating 32P-labeled glycogen with <t>laforin</t> or glucosidases. A Glycogen was labeled by incubation with 5 <t>μM</t> <t>[β-32P]UDP-glucose</t> (lower panel) or [U-14C]glucose (upper panel) and yeast glycogen synthase (2 μg/ml) for 30 min. Glycogen was precipitated with ethanol, dissolved in buffer and treated with α-glucosidases (α-amylase and amyloglucosidase) (α-G), inactive mutant laforin (C266S Laf) or wild type laforin (WT Laf) as indicated, and analyzed by SDS-Page (see Materials and Methods). Dried gels were analyzed by a Phosphorimager. C, control reaction lacking glycogen synthase; NT, not treated. B UDP-glucose and UDP were incubated with active (WT) or inactive (C266S) laforin as indicated and analyzed by HPAEC. Chromatograms of UDP, UMP and UDP-glucose standards are shown in the lowermost panel.
Expression Plasmid Pcdna3 12237, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression plasmid pcdna3 12237/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
expression plasmid pcdna3 12237 - by Bioz Stars, 2024-10
86/100 stars
  Buy from Supplier

Image Search Results


Effect of treating 32P-labeled glycogen with laforin or glucosidases. A Glycogen was labeled by incubation with 5 μM [β-32P]UDP-glucose (lower panel) or [U-14C]glucose (upper panel) and yeast glycogen synthase (2 μg/ml) for 30 min. Glycogen was precipitated with ethanol, dissolved in buffer and treated with α-glucosidases (α-amylase and amyloglucosidase) (α-G), inactive mutant laforin (C266S Laf) or wild type laforin (WT Laf) as indicated, and analyzed by SDS-Page (see Materials and Methods). Dried gels were analyzed by a Phosphorimager. C, control reaction lacking glycogen synthase; NT, not treated. B UDP-glucose and UDP were incubated with active (WT) or inactive (C266S) laforin as indicated and analyzed by HPAEC. Chromatograms of UDP, UMP and UDP-glucose standards are shown in the lowermost panel.

Journal: Archives of biochemistry and biophysics

Article Title: INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE

doi: 10.1016/j.abb.2016.03.020

Figure Lengend Snippet: Effect of treating 32P-labeled glycogen with laforin or glucosidases. A Glycogen was labeled by incubation with 5 μM [β-32P]UDP-glucose (lower panel) or [U-14C]glucose (upper panel) and yeast glycogen synthase (2 μg/ml) for 30 min. Glycogen was precipitated with ethanol, dissolved in buffer and treated with α-glucosidases (α-amylase and amyloglucosidase) (α-G), inactive mutant laforin (C266S Laf) or wild type laforin (WT Laf) as indicated, and analyzed by SDS-Page (see Materials and Methods). Dried gels were analyzed by a Phosphorimager. C, control reaction lacking glycogen synthase; NT, not treated. B UDP-glucose and UDP were incubated with active (WT) or inactive (C266S) laforin as indicated and analyzed by HPAEC. Chromatograms of UDP, UMP and UDP-glucose standards are shown in the lowermost panel.

Article Snippet: High purity UDP (99.2%) used in laforin reactions was from Chem-Impex International, Inc. (#00310).

Techniques: Labeling, Incubation, Mutagenesis, SDS Page

Release of 32Pi from 32P-glycogen by laforin. Glycogen was labeled by incubation with 5 μM [β-32P]UDP-glucose (A) or [U-14C]glucose (B) and yeast glycogen synthase (5 μg/ml) for 30 min. Glycogen was precipitated three times with ethanol, treated with PiBind™ resin, purified by gel filtration and dissolved in buffer. The glycogen was incubated for 2 hr with 50 μg/ml laforin (WT Laf) or laforin inactivated by boiling for 5 min (HI Laf). A control (C) lacked laforin. The reaction mixtures were analyzed by TLC using PEI-cellulose plates. Standards of glucose-1-P (14C-G1P), glucose (14C-Glu) and inorganic phosphate (32Pi), labeled with the indicated isotope, were also analyzed.

Journal: Archives of biochemistry and biophysics

Article Title: INCORPORATION OF PHOSPHATE INTO GLYCOGEN BY GLYCOGEN SYNTHASE

doi: 10.1016/j.abb.2016.03.020

Figure Lengend Snippet: Release of 32Pi from 32P-glycogen by laforin. Glycogen was labeled by incubation with 5 μM [β-32P]UDP-glucose (A) or [U-14C]glucose (B) and yeast glycogen synthase (5 μg/ml) for 30 min. Glycogen was precipitated three times with ethanol, treated with PiBind™ resin, purified by gel filtration and dissolved in buffer. The glycogen was incubated for 2 hr with 50 μg/ml laforin (WT Laf) or laforin inactivated by boiling for 5 min (HI Laf). A control (C) lacked laforin. The reaction mixtures were analyzed by TLC using PEI-cellulose plates. Standards of glucose-1-P (14C-G1P), glucose (14C-Glu) and inorganic phosphate (32Pi), labeled with the indicated isotope, were also analyzed.

Article Snippet: High purity UDP (99.2%) used in laforin reactions was from Chem-Impex International, Inc. (#00310).

Techniques: Labeling, Incubation, Purification, Filtration