Journal: The ISME Journal
Article Title: Acetoclastic Methanosaeta are dominant methanogens in organic-rich Antarctic marine sediments
Figure Lengend Snippet: ( a ) Representative HPLC-MS chromatogram (HILIC-ESI-MS) from site U1357, 7.15 mbsf, showing the presence of major intact polar lipid isoprenoidal glycerol dialkyl glycerol tetraethers (IPL-GDGTs) and bacterial lipids in the mass range m/z 1200–1800. The HPLC-MS chromatogram is depicted as a density map showing retention time on the x axis, m/z on the y axis and the relative peak intensity by grey shading. ( b ) Structural variety in the head groups and biphytanyl moieties of the detected IPL-GDGTs. Note that during the ether cleavage reaction the hydroxyl group in the biphytane (bp) side chain becomes dehydrated and forms biphytene (bp0:1). Position of the hydroxyl group and double bond were not determined. 1G, monohexose; 2G, dihexose; PG, phosphatidyl glycerol; HPH, hexose-phoshohexose; cren, crenarchaeol.
Article Snippet: Separation of IPL-GDGTs was achieved by reverse phase chromatography using a Zorbax Eclipse XDB C18 column (5 μm, 10 × 250 mm, Agilent Technologies, Santa Clara, CA, USA) on an Agilent 1200 series high-performance liquid chromatography (HPLC) system equipped with an Agilent 1200 series fraction collector ( ).
Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Hydrophilic Interaction Liquid Chromatography