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  • 92
    DSMZ bacillus sp
    Bacillus Sp, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacillus sp/product/DSMZ
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacillus sp - by Bioz Stars, 2021-06
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    97
    Roche immunoprecipitation kit
    G protein blocks the interaction between RIG-I and MAVS. ( A ) 293 cells were transfected with plasmids encoding Flag-tagged RIG-I-N and V5-tagged G or their control vectors. Total cell lysates were immunoprecipitated with anti-V5 antibody followed by Western blot using anti-Flag antibody to detect RIG-I-N. Reverse <t>immunoprecipitation</t> was also done, where RIG-I-N was immunoprecipitated using anti-Flag antibody and G protein was then detected using anti-V5 antibody. Total cell lysates were subjected to Western blot to determine levels of hMPV G and RIG-I-N expression. ( B ) 293 cells were transfected with a fixed amount of plasmids encoding Flag-tagged RIG-I and EGFP-tagged MAVS, or their control vectors, and increasing concentrations of a plasmid expressing V5-tagged G. Total cell lysates were immunoprecipitated with anti-Flag antibody to pull down RIG-I, followed by Western blot using anti-EGFP or anti-V5 antibody to detect associated MAVS and G, respectively. Data are representative of two independent experiments.
    Immunoprecipitation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoprecipitation kit/product/Roche
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoprecipitation kit - by Bioz Stars, 2021-06
    97/100 stars
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    86
    Roche immunoprecipitation
    G protein blocks the interaction between RIG-I and MAVS. ( A ) 293 cells were transfected with plasmids encoding Flag-tagged RIG-I-N and V5-tagged G or their control vectors. Total cell lysates were immunoprecipitated with anti-V5 antibody followed by Western blot using anti-Flag antibody to detect RIG-I-N. Reverse <t>immunoprecipitation</t> was also done, where RIG-I-N was immunoprecipitated using anti-Flag antibody and G protein was then detected using anti-V5 antibody. Total cell lysates were subjected to Western blot to determine levels of hMPV G and RIG-I-N expression. ( B ) 293 cells were transfected with a fixed amount of plasmids encoding Flag-tagged RIG-I and EGFP-tagged MAVS, or their control vectors, and increasing concentrations of a plasmid expressing V5-tagged G. Total cell lysates were immunoprecipitated with anti-Flag antibody to pull down RIG-I, followed by Western blot using anti-EGFP or anti-V5 antibody to detect associated MAVS and G, respectively. Data are representative of two independent experiments.
    Immunoprecipitation, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoprecipitation/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoprecipitation - by Bioz Stars, 2021-06
    86/100 stars
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    86
    Pfizer Inc acove 1 contract
    G protein blocks the interaction between RIG-I and MAVS. ( A ) 293 cells were transfected with plasmids encoding Flag-tagged RIG-I-N and V5-tagged G or their control vectors. Total cell lysates were immunoprecipitated with anti-V5 antibody followed by Western blot using anti-Flag antibody to detect RIG-I-N. Reverse <t>immunoprecipitation</t> was also done, where RIG-I-N was immunoprecipitated using anti-Flag antibody and G protein was then detected using anti-V5 antibody. Total cell lysates were subjected to Western blot to determine levels of hMPV G and RIG-I-N expression. ( B ) 293 cells were transfected with a fixed amount of plasmids encoding Flag-tagged RIG-I and EGFP-tagged MAVS, or their control vectors, and increasing concentrations of a plasmid expressing V5-tagged G. Total cell lysates were immunoprecipitated with anti-Flag antibody to pull down RIG-I, followed by Western blot using anti-EGFP or anti-V5 antibody to detect associated MAVS and G, respectively. Data are representative of two independent experiments.
    Acove 1 Contract, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acove 1 contract/product/Pfizer Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acove 1 contract - by Bioz Stars, 2021-06
    86/100 stars
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    Image Search Results


    G protein blocks the interaction between RIG-I and MAVS. ( A ) 293 cells were transfected with plasmids encoding Flag-tagged RIG-I-N and V5-tagged G or their control vectors. Total cell lysates were immunoprecipitated with anti-V5 antibody followed by Western blot using anti-Flag antibody to detect RIG-I-N. Reverse immunoprecipitation was also done, where RIG-I-N was immunoprecipitated using anti-Flag antibody and G protein was then detected using anti-V5 antibody. Total cell lysates were subjected to Western blot to determine levels of hMPV G and RIG-I-N expression. ( B ) 293 cells were transfected with a fixed amount of plasmids encoding Flag-tagged RIG-I and EGFP-tagged MAVS, or their control vectors, and increasing concentrations of a plasmid expressing V5-tagged G. Total cell lysates were immunoprecipitated with anti-Flag antibody to pull down RIG-I, followed by Western blot using anti-EGFP or anti-V5 antibody to detect associated MAVS and G, respectively. Data are representative of two independent experiments.

    Journal: PLoS ONE

    Article Title: Human Metapneumovirus Glycoprotein G Disrupts Mitochondrial Signaling in Airway Epithelial Cells

    doi: 10.1371/journal.pone.0062568

    Figure Lengend Snippet: G protein blocks the interaction between RIG-I and MAVS. ( A ) 293 cells were transfected with plasmids encoding Flag-tagged RIG-I-N and V5-tagged G or their control vectors. Total cell lysates were immunoprecipitated with anti-V5 antibody followed by Western blot using anti-Flag antibody to detect RIG-I-N. Reverse immunoprecipitation was also done, where RIG-I-N was immunoprecipitated using anti-Flag antibody and G protein was then detected using anti-V5 antibody. Total cell lysates were subjected to Western blot to determine levels of hMPV G and RIG-I-N expression. ( B ) 293 cells were transfected with a fixed amount of plasmids encoding Flag-tagged RIG-I and EGFP-tagged MAVS, or their control vectors, and increasing concentrations of a plasmid expressing V5-tagged G. Total cell lysates were immunoprecipitated with anti-Flag antibody to pull down RIG-I, followed by Western blot using anti-EGFP or anti-V5 antibody to detect associated MAVS and G, respectively. Data are representative of two independent experiments.

    Article Snippet: Cells were harvested 30 h post-transfection and immunoprecipitation was carried out using immunoprecipitation Kit from Roche (Cat # 11719, Indianapolis, IN) and an antibody against either V5 or Flag or an isotype antibody control, as previously described , .

    Techniques: Transfection, Immunoprecipitation, Western Blot, Expressing, Plasmid Preparation