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  • 93
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    GATA3 induces the development of ILC progenitors. Five-day expanded UCB CD34 + α4β7 − HSCs were transfected with either GATA3 mRNA (GATA3) or scrambled mRNA (control) and differentiated under conditions that favor the development of ILCs (A) Gating strategy, 5-days expanded CD34 + α4β7 − HSCs were sorted by FACS. (B) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of α4β7 + cells. (C,D) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and the percentage (C) and MFI (D) of α4β7 + cells is shown ( n = 4/group). (E) CD34 + α4β7 − HSCs were stained for the surface <t>CD127</t> at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD127 + cells. (F) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and the percentage of CD127 + cells is shown in bar graph ( n = 4/group). (G) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD117 + CD11a − ILCPs. (H) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and the percentage of CD117 + CD11a − ILCPs is shown in bar graph ( n = 4/group). (I) Total counts of transfected and differentiating HSCs were performed at day 14 of culture and live cells in 1 mL of culture media is depicted ( n = 4/group). (J) Absolute counts of CD117 + CD11a − ILCPs in 1 mL of culture media is depicted ( n = 4/group). (C,D,F,H–J) , Data are shown as means ± SD, and p -values are depicted and paired two-tailed t -tests.
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    GATA3 induces the development of ILC progenitors. Five-day expanded UCB CD34 + α4β7 − HSCs were transfected with either GATA3 mRNA (GATA3) or scrambled mRNA (control) and differentiated under conditions that favor the development of ILCs (A) Gating strategy, 5-days expanded CD34 + α4β7 − HSCs were sorted by FACS. (B) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of α4β7 + cells. (C,D) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and the percentage (C) and MFI (D) of α4β7 + cells is shown ( n = 4/group). (E) CD34 + α4β7 − HSCs were stained for the surface <t>CD127</t> at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD127 + cells. (F) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and the percentage of CD127 + cells is shown in bar graph ( n = 4/group). (G) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD117 + CD11a − ILCPs. (H) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and the percentage of CD117 + CD11a − ILCPs is shown in bar graph ( n = 4/group). (I) Total counts of transfected and differentiating HSCs were performed at day 14 of culture and live cells in 1 mL of culture media is depicted ( n = 4/group). (J) Absolute counts of CD117 + CD11a − ILCPs in 1 mL of culture media is depicted ( n = 4/group). (C,D,F,H–J) , Data are shown as means ± SD, and p -values are depicted and paired two-tailed t -tests.
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    Image Search Results


    GATA3 induces the development of ILC progenitors. Five-day expanded UCB CD34 + α4β7 − HSCs were transfected with either GATA3 mRNA (GATA3) or scrambled mRNA (control) and differentiated under conditions that favor the development of ILCs (A) Gating strategy, 5-days expanded CD34 + α4β7 − HSCs were sorted by FACS. (B) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of α4β7 + cells. (C,D) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and the percentage (C) and MFI (D) of α4β7 + cells is shown ( n = 4/group). (E) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD127 + cells. (F) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and the percentage of CD127 + cells is shown in bar graph ( n = 4/group). (G) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD117 + CD11a − ILCPs. (H) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and the percentage of CD117 + CD11a − ILCPs is shown in bar graph ( n = 4/group). (I) Total counts of transfected and differentiating HSCs were performed at day 14 of culture and live cells in 1 mL of culture media is depicted ( n = 4/group). (J) Absolute counts of CD117 + CD11a − ILCPs in 1 mL of culture media is depicted ( n = 4/group). (C,D,F,H–J) , Data are shown as means ± SD, and p -values are depicted and paired two-tailed t -tests.

    Journal: Frontiers in Immunology

    Article Title: Transient Expression of GATA3 in Hematopoietic Stem Cells Facilitates Helper Innate Lymphoid Cell Differentiation

    doi: 10.3389/fimmu.2019.00510

    Figure Lengend Snippet: GATA3 induces the development of ILC progenitors. Five-day expanded UCB CD34 + α4β7 − HSCs were transfected with either GATA3 mRNA (GATA3) or scrambled mRNA (control) and differentiated under conditions that favor the development of ILCs (A) Gating strategy, 5-days expanded CD34 + α4β7 − HSCs were sorted by FACS. (B) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of α4β7 + cells. (C,D) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and the percentage (C) and MFI (D) of α4β7 + cells is shown ( n = 4/group). (E) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD127 + cells. (F) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and the percentage of CD127 + cells is shown in bar graph ( n = 4/group). (G) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD117 + CD11a − ILCPs. (H) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and the percentage of CD117 + CD11a − ILCPs is shown in bar graph ( n = 4/group). (I) Total counts of transfected and differentiating HSCs were performed at day 14 of culture and live cells in 1 mL of culture media is depicted ( n = 4/group). (J) Absolute counts of CD117 + CD11a − ILCPs in 1 mL of culture media is depicted ( n = 4/group). (C,D,F,H–J) , Data are shown as means ± SD, and p -values are depicted and paired two-tailed t -tests.

    Article Snippet: Expression of surface receptor was determined using the following monoclonal antibodies: anti-CD3-PerCp5.5 (clone HIT3a), anti-CD11c-PerCp5.5 (clone Bu15), anti-D14-PerCp5.5 (clone 63D3), anti-CD19-PerCp5.5 (clone HIB19), anti-CD123-PerCp5.5 (clone 6H6), anti-CD127-PE (clone A019D5), anti-CD294-BV421/BV605 (clone BM16), anti-CD336-APC (clone P44-8), and anti-mouse IgG-FITC/PE (clone Poly4053) (all from Biolegend); anti-CD11a-FITC (clone G43-25B), anti-CD34-PE/APC (clone 4H11), anti-CD45-APC/PE (clone HI30), anti-CD56-BV421/BV605 (clone NCAM16.2), anti-CD94-PerCp5.5/FITC (clone HP-3D9), anti-CD117-PE-Cy7 (clone 104D2), and anti-CD161-PE (clone DX12) (all from BD Biosciences); and anti-α4β7 (Cat#11718, from NIH).

    Techniques: Transfection, FACS, Staining, Electroporation, Two Tailed Test