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  • 96
    Cayman Chemical jasplakinolide
    Subcellular cytoskeleton (CSK) tension and focal adhesion (FA) followed distinct mechanosensitive rheostasis to drive single-cell mechanical homeostasis. ( a b ) Heterogeneous rheostatic paths for subcellular CSK tension ( a ) and FA ( b ). Results from REF-52 fibroblasts under 8% static equibiaxial stretch were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Average result from each subset was plotted using the rainbow spectrum (from purple to red ). Whole-cell average response ( black ) was included for referencing single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( c-f ) Dependence of subcellular rheostasis on ground-state values of CSK tension and FA size. Changes in CSK tension ( c d ) and FA size ( e f ) during Phase I ( t = 0 - 1 min; c e ) and phase II ( t = 1 - 30 min; d f ) were color-coded in two-dimensional ground-state CSK tension - FA size diagrams obtained at t = 0 min. Red dashed lines in d and f marked average ground-state values of CSK tension and FA size as well as a transition boundary between reinforcement and relaxation for subcellular rheostasis during Phase II . ( g-j ) Responses of subcellular CSK tension ( top ) and FA ( bottom ) rheostasis to pharmacological inhibitors in REF-52 fibroblasts under 8% static equibiaxial stretch ( g : nocodazole; h : blebbistatin; i : <t>jasplakinolide;</t> j : cytochalasin D). Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average responses ( black ) were included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( k ) Force - lifetime diagram of the catch-slip bond between α 5 β 1 integrin and FNIII 7-10 , with or without treatments of antibody TS2/16 as indicated (from Ref. [ 23 ]). ( l ) Response of subcellular CSK tension ( left ) and FA ( right ) rheostasis to TS2/16 treatment in human mesenchymal stem cells (HUMSCs) under 8% static equibiaxial stretch. Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average response ( black ) was included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 11. In g-i and l , hollow red arrowheads indicated suppressed FA reinforcement in Phase I , and solid red arrowheads marked suppressed FA relaxation in Phase II . P -values were calculated using student’s paired sample t -test comparing data before ( t = 0 min) and after ( t = 1 or 30 min) stretch. N.S. , statistically not significant and P > 0.05. *, P
    Jasplakinolide, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jasplakinolide/product/Cayman Chemical
    Average 96 stars, based on 1 article reviews
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    86
    Santa Cruz Biotechnology anti mis
    Subcellular cytoskeleton (CSK) tension and focal adhesion (FA) followed distinct mechanosensitive rheostasis to drive single-cell mechanical homeostasis. ( a b ) Heterogeneous rheostatic paths for subcellular CSK tension ( a ) and FA ( b ). Results from REF-52 fibroblasts under 8% static equibiaxial stretch were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Average result from each subset was plotted using the rainbow spectrum (from purple to red ). Whole-cell average response ( black ) was included for referencing single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( c-f ) Dependence of subcellular rheostasis on ground-state values of CSK tension and FA size. Changes in CSK tension ( c d ) and FA size ( e f ) during Phase I ( t = 0 - 1 min; c e ) and phase II ( t = 1 - 30 min; d f ) were color-coded in two-dimensional ground-state CSK tension - FA size diagrams obtained at t = 0 min. Red dashed lines in d and f marked average ground-state values of CSK tension and FA size as well as a transition boundary between reinforcement and relaxation for subcellular rheostasis during Phase II . ( g-j ) Responses of subcellular CSK tension ( top ) and FA ( bottom ) rheostasis to pharmacological inhibitors in REF-52 fibroblasts under 8% static equibiaxial stretch ( g : nocodazole; h : blebbistatin; i : <t>jasplakinolide;</t> j : cytochalasin D). Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average responses ( black ) were included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( k ) Force - lifetime diagram of the catch-slip bond between α 5 β 1 integrin and FNIII 7-10 , with or without treatments of antibody TS2/16 as indicated (from Ref. [ 23 ]). ( l ) Response of subcellular CSK tension ( left ) and FA ( right ) rheostasis to TS2/16 treatment in human mesenchymal stem cells (HUMSCs) under 8% static equibiaxial stretch. Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average response ( black ) was included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 11. In g-i and l , hollow red arrowheads indicated suppressed FA reinforcement in Phase I , and solid red arrowheads marked suppressed FA relaxation in Phase II . P -values were calculated using student’s paired sample t -test comparing data before ( t = 0 min) and after ( t = 1 or 30 min) stretch. N.S. , statistically not significant and P > 0.05. *, P
    Anti Mis, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mis/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
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    93
    Millipore baird parker agar
    Subcellular cytoskeleton (CSK) tension and focal adhesion (FA) followed distinct mechanosensitive rheostasis to drive single-cell mechanical homeostasis. ( a b ) Heterogeneous rheostatic paths for subcellular CSK tension ( a ) and FA ( b ). Results from REF-52 fibroblasts under 8% static equibiaxial stretch were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Average result from each subset was plotted using the rainbow spectrum (from purple to red ). Whole-cell average response ( black ) was included for referencing single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( c-f ) Dependence of subcellular rheostasis on ground-state values of CSK tension and FA size. Changes in CSK tension ( c d ) and FA size ( e f ) during Phase I ( t = 0 - 1 min; c e ) and phase II ( t = 1 - 30 min; d f ) were color-coded in two-dimensional ground-state CSK tension - FA size diagrams obtained at t = 0 min. Red dashed lines in d and f marked average ground-state values of CSK tension and FA size as well as a transition boundary between reinforcement and relaxation for subcellular rheostasis during Phase II . ( g-j ) Responses of subcellular CSK tension ( top ) and FA ( bottom ) rheostasis to pharmacological inhibitors in REF-52 fibroblasts under 8% static equibiaxial stretch ( g : nocodazole; h : blebbistatin; i : <t>jasplakinolide;</t> j : cytochalasin D). Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average responses ( black ) were included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( k ) Force - lifetime diagram of the catch-slip bond between α 5 β 1 integrin and FNIII 7-10 , with or without treatments of antibody TS2/16 as indicated (from Ref. [ 23 ]). ( l ) Response of subcellular CSK tension ( left ) and FA ( right ) rheostasis to TS2/16 treatment in human mesenchymal stem cells (HUMSCs) under 8% static equibiaxial stretch. Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average response ( black ) was included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 11. In g-i and l , hollow red arrowheads indicated suppressed FA reinforcement in Phase I , and solid red arrowheads marked suppressed FA relaxation in Phase II . P -values were calculated using student’s paired sample t -test comparing data before ( t = 0 min) and after ( t = 1 or 30 min) stretch. N.S. , statistically not significant and P > 0.05. *, P
    Baird Parker Agar, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher antibodies against il 27
    Mice lacking <t>IL-27/WSX-1</t> signalling still develop mechanical hypersensitivity after inflammation and peripheral nerve injury. Mice were subjected to the ( a – c) inflammatory or ( d – g ) neuropathic pain model. Fifty percent paw withdrawal threshold before and after ( a ) CFA injection or ( d ) spinal nerve injury. ( b , e ) The percent change in paw withdrawal threshold calculated from a and d, respectively. Each value after CFA injection or nerve injury was normalised by the control value (pre) in each mouse. ( c ) Hind paw weight, as a measure of oedema, in mice 14 days after i.pl. CFA injection. ( f ) Representative immunofluorescence labelling for Iba1 (green) with nuclear staining with TO-PRO-3 (blue) in a transverse section of the dorsal horns from the L4 segment of WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. ( g ) Number of Iba1-positive cells in the ipsilateral or contralateral dorsal horn of L4 spinal segments dissected from WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. n = 5 animals/group. All data are expressed as means ± SEM.
    Antibodies Against Il 27, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Subcellular cytoskeleton (CSK) tension and focal adhesion (FA) followed distinct mechanosensitive rheostasis to drive single-cell mechanical homeostasis. ( a b ) Heterogeneous rheostatic paths for subcellular CSK tension ( a ) and FA ( b ). Results from REF-52 fibroblasts under 8% static equibiaxial stretch were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Average result from each subset was plotted using the rainbow spectrum (from purple to red ). Whole-cell average response ( black ) was included for referencing single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( c-f ) Dependence of subcellular rheostasis on ground-state values of CSK tension and FA size. Changes in CSK tension ( c d ) and FA size ( e f ) during Phase I ( t = 0 - 1 min; c e ) and phase II ( t = 1 - 30 min; d f ) were color-coded in two-dimensional ground-state CSK tension - FA size diagrams obtained at t = 0 min. Red dashed lines in d and f marked average ground-state values of CSK tension and FA size as well as a transition boundary between reinforcement and relaxation for subcellular rheostasis during Phase II . ( g-j ) Responses of subcellular CSK tension ( top ) and FA ( bottom ) rheostasis to pharmacological inhibitors in REF-52 fibroblasts under 8% static equibiaxial stretch ( g : nocodazole; h : blebbistatin; i : jasplakinolide; j : cytochalasin D). Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average responses ( black ) were included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( k ) Force - lifetime diagram of the catch-slip bond between α 5 β 1 integrin and FNIII 7-10 , with or without treatments of antibody TS2/16 as indicated (from Ref. [ 23 ]). ( l ) Response of subcellular CSK tension ( left ) and FA ( right ) rheostasis to TS2/16 treatment in human mesenchymal stem cells (HUMSCs) under 8% static equibiaxial stretch. Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average response ( black ) was included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 11. In g-i and l , hollow red arrowheads indicated suppressed FA reinforcement in Phase I , and solid red arrowheads marked suppressed FA relaxation in Phase II . P -values were calculated using student’s paired sample t -test comparing data before ( t = 0 min) and after ( t = 1 or 30 min) stretch. N.S. , statistically not significant and P > 0.05. *, P

    Journal: Nature materials

    Article Title: Mechanosensitive subcellular rheostasis drives emergent single-cell mechanical homeostasis

    doi: 10.1038/nmat4654

    Figure Lengend Snippet: Subcellular cytoskeleton (CSK) tension and focal adhesion (FA) followed distinct mechanosensitive rheostasis to drive single-cell mechanical homeostasis. ( a b ) Heterogeneous rheostatic paths for subcellular CSK tension ( a ) and FA ( b ). Results from REF-52 fibroblasts under 8% static equibiaxial stretch were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Average result from each subset was plotted using the rainbow spectrum (from purple to red ). Whole-cell average response ( black ) was included for referencing single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( c-f ) Dependence of subcellular rheostasis on ground-state values of CSK tension and FA size. Changes in CSK tension ( c d ) and FA size ( e f ) during Phase I ( t = 0 - 1 min; c e ) and phase II ( t = 1 - 30 min; d f ) were color-coded in two-dimensional ground-state CSK tension - FA size diagrams obtained at t = 0 min. Red dashed lines in d and f marked average ground-state values of CSK tension and FA size as well as a transition boundary between reinforcement and relaxation for subcellular rheostasis during Phase II . ( g-j ) Responses of subcellular CSK tension ( top ) and FA ( bottom ) rheostasis to pharmacological inhibitors in REF-52 fibroblasts under 8% static equibiaxial stretch ( g : nocodazole; h : blebbistatin; i : jasplakinolide; j : cytochalasin D). Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average responses ( black ) were included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 10. ( k ) Force - lifetime diagram of the catch-slip bond between α 5 β 1 integrin and FNIII 7-10 , with or without treatments of antibody TS2/16 as indicated (from Ref. [ 23 ]). ( l ) Response of subcellular CSK tension ( left ) and FA ( right ) rheostasis to TS2/16 treatment in human mesenchymal stem cells (HUMSCs) under 8% static equibiaxial stretch. Results were grouped into subsets based on ground-state CSK tension values at t = 0 min ( F 0 ). Whole-cell average response ( black ) was included to indicate changes in single-cell homeostasis. Data represents the mean ± s.e.m with n = 11. In g-i and l , hollow red arrowheads indicated suppressed FA reinforcement in Phase I , and solid red arrowheads marked suppressed FA relaxation in Phase II . P -values were calculated using student’s paired sample t -test comparing data before ( t = 0 min) and after ( t = 1 or 30 min) stretch. N.S. , statistically not significant and P > 0.05. *, P

    Article Snippet: To perturb the actin cytoskeleton (CSK) in REF-52 fibroblasts, small-molecule inhibitors targeting the CSK integrity and tension, including 50 nM nocodazole (Sigma-Aldrich), which depolymerizes microtubules and impedes FA disassembly ; 10 μM blebbistatin (Cayman Chemical, Ann Arbor, MI), which inhibits myosin motor activity and thus CSK tension ; 10 nM jasplakinolide (Cayman Chemical), which enhances actin polymerization ; and 200 nM cytochalasin D (Tocris bioscience, Bristol, UK), which blocks actin polymerization , were supplemented to cell growth medium for 2 hr prior to cell stretch assays.

    Techniques:

    Mice lacking IL-27/WSX-1 signalling still develop mechanical hypersensitivity after inflammation and peripheral nerve injury. Mice were subjected to the ( a – c) inflammatory or ( d – g ) neuropathic pain model. Fifty percent paw withdrawal threshold before and after ( a ) CFA injection or ( d ) spinal nerve injury. ( b , e ) The percent change in paw withdrawal threshold calculated from a and d, respectively. Each value after CFA injection or nerve injury was normalised by the control value (pre) in each mouse. ( c ) Hind paw weight, as a measure of oedema, in mice 14 days after i.pl. CFA injection. ( f ) Representative immunofluorescence labelling for Iba1 (green) with nuclear staining with TO-PRO-3 (blue) in a transverse section of the dorsal horns from the L4 segment of WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. ( g ) Number of Iba1-positive cells in the ipsilateral or contralateral dorsal horn of L4 spinal segments dissected from WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. n = 5 animals/group. All data are expressed as means ± SEM.

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: Mice lacking IL-27/WSX-1 signalling still develop mechanical hypersensitivity after inflammation and peripheral nerve injury. Mice were subjected to the ( a – c) inflammatory or ( d – g ) neuropathic pain model. Fifty percent paw withdrawal threshold before and after ( a ) CFA injection or ( d ) spinal nerve injury. ( b , e ) The percent change in paw withdrawal threshold calculated from a and d, respectively. Each value after CFA injection or nerve injury was normalised by the control value (pre) in each mouse. ( c ) Hind paw weight, as a measure of oedema, in mice 14 days after i.pl. CFA injection. ( f ) Representative immunofluorescence labelling for Iba1 (green) with nuclear staining with TO-PRO-3 (blue) in a transverse section of the dorsal horns from the L4 segment of WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. ( g ) Number of Iba1-positive cells in the ipsilateral or contralateral dorsal horn of L4 spinal segments dissected from WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. n = 5 animals/group. All data are expressed as means ± SEM.

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Mouse Assay, Injection, Immunofluorescence, Staining

    Facilitated mechanical sensitivity of C-fibre nociceptors in mice lacking IL-27/WSX-1 signalling. ( a ) Sample recordings of peri-stimulus time histogram. Abscissa: time in seconds. Ordinate: discharge rate of C-nociceptors. Lowest trace: force output of the mechanical stimulus. ( b ) Summarised mechanical response patterns. The response magnitude was significantly greater in WSX-1 −/− , EBI3 −/− , and p28 −/− mice compared with WT. ( a , b ) WT (n = 28), WSX-1 −/− (n = 29), EBI3 −/− (n = 25), p28 −/− (n = 19). P

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: Facilitated mechanical sensitivity of C-fibre nociceptors in mice lacking IL-27/WSX-1 signalling. ( a ) Sample recordings of peri-stimulus time histogram. Abscissa: time in seconds. Ordinate: discharge rate of C-nociceptors. Lowest trace: force output of the mechanical stimulus. ( b ) Summarised mechanical response patterns. The response magnitude was significantly greater in WSX-1 −/− , EBI3 −/− , and p28 −/− mice compared with WT. ( a , b ) WT (n = 28), WSX-1 −/− (n = 29), EBI3 −/− (n = 25), p28 −/− (n = 19). P

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Mouse Assay

    Lack of IL-27/WSX-1 signalling results in enhanced nociceptive behaviours. Reaction latencies to heat measured with the ( a ) hot-plate and ( b ) tail-flick tests. ( c ) Acetone test, in which values represent reaction scores after acetone application to a hind paw. ( d ) Fifty percent paw withdrawal threshold measured with the von Frey test. ( e ) Duration of licking and biting during the first (0–10 min) and second (10–60 min) phases after formalin injection into a hind paw. ( f ) Duration of licking and biting after capsaicin injection into a hind paw. n = 5–10. * P

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: Lack of IL-27/WSX-1 signalling results in enhanced nociceptive behaviours. Reaction latencies to heat measured with the ( a ) hot-plate and ( b ) tail-flick tests. ( c ) Acetone test, in which values represent reaction scores after acetone application to a hind paw. ( d ) Fifty percent paw withdrawal threshold measured with the von Frey test. ( e ) Duration of licking and biting during the first (0–10 min) and second (10–60 min) phases after formalin injection into a hind paw. ( f ) Duration of licking and biting after capsaicin injection into a hind paw. n = 5–10. * P

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Tail Flick Test, Injection

    Intraplantar blockade of IL-27 decreased paw withdrawal threshold in naïve WT mice. Fifty percent paw withdrawal threshold before and after i.pl. injection of a neutralising antibody against p28 or control IgG into WT mice. n = 3–5 animals/group. * P

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: Intraplantar blockade of IL-27 decreased paw withdrawal threshold in naïve WT mice. Fifty percent paw withdrawal threshold before and after i.pl. injection of a neutralising antibody against p28 or control IgG into WT mice. n = 3–5 animals/group. * P

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Mouse Assay, Injection

    IL-27 is an endogenous regulator of thermal and mechanical nociceptive responses. ( a ) Single i.p. injection of rIL-27 dose-dependently prolonged hot-plate latency in EBI3 −/− mice. ( b ) Quick restoration of hot-plate latencies in p28 −/− , but not WSX-1 −/− and WT mice following a single i.p. injection of rIL-27 (16 µg/kg). ( c ) A single i.p. injection of rIL-27 (16 µg/kg) also significantly alleviated mechanical hypersensitivity in EBI3 −/− mice. ( d ) A single i.p. injection of neutralising antibody against p28 (1.6 mg/kg) decreased the paw withdrawal threshold in naïve WT mice. n = 5 animals/group. * P

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: IL-27 is an endogenous regulator of thermal and mechanical nociceptive responses. ( a ) Single i.p. injection of rIL-27 dose-dependently prolonged hot-plate latency in EBI3 −/− mice. ( b ) Quick restoration of hot-plate latencies in p28 −/− , but not WSX-1 −/− and WT mice following a single i.p. injection of rIL-27 (16 µg/kg). ( c ) A single i.p. injection of rIL-27 (16 µg/kg) also significantly alleviated mechanical hypersensitivity in EBI3 −/− mice. ( d ) A single i.p. injection of neutralising antibody against p28 (1.6 mg/kg) decreased the paw withdrawal threshold in naïve WT mice. n = 5 animals/group. * P

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Injection, Mouse Assay