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  • 97
    Polyplus Transfection dna transfection
    Dna Transfection, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dna transfection - by Bioz Stars, 2021-05
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    93
    Santa Cruz Biotechnology ang 2
    Mechanisms involved in MUC4/Y-AMOP domain regulating angiogenesis and metastasis. a Effects of MUC4/Y and its AMOP domain on the NOTCH3 signalling pathway (NOTCH3, N3ICD, HES-1) were assayed by western blotting. GAPDH was used as the internal control. b Immunofluorescence was used to assess the NOTCH3 and MUC4(MUC/Y) protein level. c Western blotting assays were conducted to analyse the protein level of VEGF-A, MMP-9, <t>ANG-2</t> and N3ICD in different groups. GAPDH was used as the internal control. d The secreted level of VEGF-A in conditioned medium was detected by an ELISA kit assay. e The activity of MMP-9 in different groups was detected by using a Fluorokine E Human MMP-9 Activity Assay kit assay. f The mRNA expression of ANG-2 in different groups. DAPT: γ-secretase inhibitors (GSI) can inhibit the proteolytic processing of Notch receptors by γ-secretase, which is essential for Notch activation. ** P
    Ang 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ang 2/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
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    ang 2 - by Bioz Stars, 2021-05
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    Mechanisms involved in MUC4/Y-AMOP domain regulating angiogenesis and metastasis. a Effects of MUC4/Y and its AMOP domain on the NOTCH3 signalling pathway (NOTCH3, N3ICD, HES-1) were assayed by western blotting. GAPDH was used as the internal control. b Immunofluorescence was used to assess the NOTCH3 and MUC4(MUC/Y) protein level. c Western blotting assays were conducted to analyse the protein level of VEGF-A, MMP-9, ANG-2 and N3ICD in different groups. GAPDH was used as the internal control. d The secreted level of VEGF-A in conditioned medium was detected by an ELISA kit assay. e The activity of MMP-9 in different groups was detected by using a Fluorokine E Human MMP-9 Activity Assay kit assay. f The mRNA expression of ANG-2 in different groups. DAPT: γ-secretase inhibitors (GSI) can inhibit the proteolytic processing of Notch receptors by γ-secretase, which is essential for Notch activation. ** P

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer

    doi: 10.1186/s13046-016-0369-0

    Figure Lengend Snippet: Mechanisms involved in MUC4/Y-AMOP domain regulating angiogenesis and metastasis. a Effects of MUC4/Y and its AMOP domain on the NOTCH3 signalling pathway (NOTCH3, N3ICD, HES-1) were assayed by western blotting. GAPDH was used as the internal control. b Immunofluorescence was used to assess the NOTCH3 and MUC4(MUC/Y) protein level. c Western blotting assays were conducted to analyse the protein level of VEGF-A, MMP-9, ANG-2 and N3ICD in different groups. GAPDH was used as the internal control. d The secreted level of VEGF-A in conditioned medium was detected by an ELISA kit assay. e The activity of MMP-9 in different groups was detected by using a Fluorokine E Human MMP-9 Activity Assay kit assay. f The mRNA expression of ANG-2 in different groups. DAPT: γ-secretase inhibitors (GSI) can inhibit the proteolytic processing of Notch receptors by γ-secretase, which is essential for Notch activation. ** P

    Article Snippet: The antibodies to ANG-2 (SC-7015) and GAPDH (SC-365062) were purchased from Santa Cruz Biotechnology (USA).

    Techniques: Western Blot, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activity Assay, Expressing, Activation Assay