Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer
Figure Lengend Snippet: Mechanisms involved in MUC4/Y-AMOP domain regulating angiogenesis and metastasis. a Effects of MUC4/Y and its AMOP domain on the NOTCH3 signalling pathway (NOTCH3, N3ICD, HES-1) were assayed by western blotting. GAPDH was used as the internal control. b Immunofluorescence was used to assess the NOTCH3 and MUC4(MUC/Y) protein level. c Western blotting assays were conducted to analyse the protein level of VEGF-A, MMP-9, ANG-2 and N3ICD in different groups. GAPDH was used as the internal control. d The secreted level of VEGF-A in conditioned medium was detected by an ELISA kit assay. e The activity of MMP-9 in different groups was detected by using a Fluorokine E Human MMP-9 Activity Assay kit assay. f The mRNA expression of ANG-2 in different groups. DAPT: γ-secretase inhibitors (GSI) can inhibit the proteolytic processing of Notch receptors by γ-secretase, which is essential for Notch activation. ** P
Article Snippet: The antibodies to ANG-2 (SC-7015) and GAPDH (SC-365062) were purchased from Santa Cruz Biotechnology (USA).
Techniques: Western Blot, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activity Assay, Expressing, Activation Assay