11-356 Search Results


ere luc  (Addgene inc)
85
Addgene inc ere luc
LKB1 enhances ERα activity in the presence of 17-β estradiol. (A) G361 cells were transfected with LKB1, ERα, <t>ERE-luc,</t> and pRL-tk expression plasmids or vector (V), left untreated or treated with E2 as described in Materials and Methods, followed by reporter assays. Data representative of two experiments in triplicate mean ± SD. (B) G361 cells were transfected with ERα, ERE-luc, and pRL-tk expression plasmids, and increasing concentrations of LKB1 expression plasmid, left untreated, or treated with E2 or (C) 4-OHT as described in Materials and Methods followed by reporter assays. Results are three experiments in triplicate. Data are mean ± SEM. *p < 0.02 compared with 0 ng; **p < 0.05 compared with 0 ng. (D and E) MCF7 cells were transfected and treated as described in B and C. Results are three experiments in triplicate. Data are mean ± SEM. (F) MCF7 cells were transfected with three different siRNA-LKB1 duplexes, ERE-luc, pRL-tk, and treated with E2 as described in Materials and Methods followed, by reporter assays. Western blot analysis confirms abrogation of LKB1 expression by using anti-LKB1, -ERα, and -actin antibodies. Results are representative of two experiments in triplicate mean ± SD.
Ere Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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11356 issn  (Thermo Fisher)
86
Thermo Fisher 11356 issn
LKB1 enhances ERα activity in the presence of 17-β estradiol. (A) G361 cells were transfected with LKB1, ERα, <t>ERE-luc,</t> and pRL-tk expression plasmids or vector (V), left untreated or treated with E2 as described in Materials and Methods, followed by reporter assays. Data representative of two experiments in triplicate mean ± SD. (B) G361 cells were transfected with ERα, ERE-luc, and pRL-tk expression plasmids, and increasing concentrations of LKB1 expression plasmid, left untreated, or treated with E2 or (C) 4-OHT as described in Materials and Methods followed by reporter assays. Results are three experiments in triplicate. Data are mean ± SEM. *p < 0.02 compared with 0 ng; **p < 0.05 compared with 0 ng. (D and E) MCF7 cells were transfected and treated as described in B and C. Results are three experiments in triplicate. Data are mean ± SEM. (F) MCF7 cells were transfected with three different siRNA-LKB1 duplexes, ERE-luc, pRL-tk, and treated with E2 as described in Materials and Methods followed, by reporter assays. Western blot analysis confirms abrogation of LKB1 expression by using anti-LKB1, -ERα, and -actin antibodies. Results are representative of two experiments in triplicate mean ± SD.
11356 Issn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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angewandte zuschriften 11356  (Verlag GmbH)
86
Verlag GmbH angewandte zuschriften 11356
LKB1 enhances ERα activity in the presence of 17-β estradiol. (A) G361 cells were transfected with LKB1, ERα, <t>ERE-luc,</t> and pRL-tk expression plasmids or vector (V), left untreated or treated with E2 as described in Materials and Methods, followed by reporter assays. Data representative of two experiments in triplicate mean ± SD. (B) G361 cells were transfected with ERα, ERE-luc, and pRL-tk expression plasmids, and increasing concentrations of LKB1 expression plasmid, left untreated, or treated with E2 or (C) 4-OHT as described in Materials and Methods followed by reporter assays. Results are three experiments in triplicate. Data are mean ± SEM. *p < 0.02 compared with 0 ng; **p < 0.05 compared with 0 ng. (D and E) MCF7 cells were transfected and treated as described in B and C. Results are three experiments in triplicate. Data are mean ± SEM. (F) MCF7 cells were transfected with three different siRNA-LKB1 duplexes, ERE-luc, pRL-tk, and treated with E2 as described in Materials and Methods followed, by reporter assays. Western blot analysis confirms abrogation of LKB1 expression by using anti-LKB1, -ERα, and -actin antibodies. Results are representative of two experiments in triplicate mean ± SD.
Angewandte Zuschriften 11356, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


LKB1 enhances ERα activity in the presence of 17-β estradiol. (A) G361 cells were transfected with LKB1, ERα, ERE-luc, and pRL-tk expression plasmids or vector (V), left untreated or treated with E2 as described in Materials and Methods, followed by reporter assays. Data representative of two experiments in triplicate mean ± SD. (B) G361 cells were transfected with ERα, ERE-luc, and pRL-tk expression plasmids, and increasing concentrations of LKB1 expression plasmid, left untreated, or treated with E2 or (C) 4-OHT as described in Materials and Methods followed by reporter assays. Results are three experiments in triplicate. Data are mean ± SEM. *p < 0.02 compared with 0 ng; **p < 0.05 compared with 0 ng. (D and E) MCF7 cells were transfected and treated as described in B and C. Results are three experiments in triplicate. Data are mean ± SEM. (F) MCF7 cells were transfected with three different siRNA-LKB1 duplexes, ERE-luc, pRL-tk, and treated with E2 as described in Materials and Methods followed, by reporter assays. Western blot analysis confirms abrogation of LKB1 expression by using anti-LKB1, -ERα, and -actin antibodies. Results are representative of two experiments in triplicate mean ± SD.

Journal:

Article Title: LKB1 Catalytic Activity Contributes to Estrogen Receptor ? Signaling

doi: 10.1091/mbc.E08-11-1138

Figure Lengend Snippet: LKB1 enhances ERα activity in the presence of 17-β estradiol. (A) G361 cells were transfected with LKB1, ERα, ERE-luc, and pRL-tk expression plasmids or vector (V), left untreated or treated with E2 as described in Materials and Methods, followed by reporter assays. Data representative of two experiments in triplicate mean ± SD. (B) G361 cells were transfected with ERα, ERE-luc, and pRL-tk expression plasmids, and increasing concentrations of LKB1 expression plasmid, left untreated, or treated with E2 or (C) 4-OHT as described in Materials and Methods followed by reporter assays. Results are three experiments in triplicate. Data are mean ± SEM. *p < 0.02 compared with 0 ng; **p < 0.05 compared with 0 ng. (D and E) MCF7 cells were transfected and treated as described in B and C. Results are three experiments in triplicate. Data are mean ± SEM. (F) MCF7 cells were transfected with three different siRNA-LKB1 duplexes, ERE-luc, pRL-tk, and treated with E2 as described in Materials and Methods followed, by reporter assays. Western blot analysis confirms abrogation of LKB1 expression by using anti-LKB1, -ERα, and -actin antibodies. Results are representative of two experiments in triplicate mean ± SD.

Article Snippet: Briefly, MCF7 or G361 cells were transfected with the following plasmids: LKB1 (50 ng), D194A/R304W (50 ng) LKB1 NR mutants (50 ng), pRL-tk (0.5 ng), ERE-luc (gift from Dr. Myles Brown, Harvard University, Boston, MA)/cyclin D1-luc (50 ng), ERα/ERβ (Addgene plasmid 11356) (10 ng)/androgen receptor (AR; 10 ng), glucocorticoid receptor (GR; 50 ng), mouse mammary tumor virus luciferase (MMTV-luc; 50 ng) (gifts from Dr. Steven Balk, Harvard University), p300 (50 ng) (Addgene plasmid 10718; Addgene, Cambridge, MA), BRCA1, and p53 (indicated concentrations).

Techniques: Activity Assay, Transfection, Expressing, Plasmid Preparation, Western Blot

LKB1 and p300 synergize to enhance ERα activity. (A) Left, G361 cells were transfected with increasing concentrations of p300 expression plasmids as indicated; ERα, ERE-luc, and pRL-tk, or vector (V); left untreated or treated with E2 for 24 h followed by reporter gene assays. (A) Right, G361 cells were transfected with increasing concentrations of p300 expression plasmids, a constant concentration of LKB1 as indicated; ERα, ERE-luc, and pRL-tk or V; left untreated or treated with E2 for 24 h followed by reporter gene assays. Results are two experiments in triplicate. Data are mean ± SD (B) G361 cells were transfected with pRL-tk, ERα, ERE-luc, and increasing concentrations of p53, or LKB1 expression plasmids, left untreated or treated with E2 for 24 h followed by reporter gene assays. Data representative of three experiments in triplicate. Data are mean ± SD. (C) Transfection conditions similar to B; however, in the presence of increasing concentrations of BRCA1. Data are representative of three experiments in triplicate mean ± SD.

Journal:

Article Title: LKB1 Catalytic Activity Contributes to Estrogen Receptor ? Signaling

doi: 10.1091/mbc.E08-11-1138

Figure Lengend Snippet: LKB1 and p300 synergize to enhance ERα activity. (A) Left, G361 cells were transfected with increasing concentrations of p300 expression plasmids as indicated; ERα, ERE-luc, and pRL-tk, or vector (V); left untreated or treated with E2 for 24 h followed by reporter gene assays. (A) Right, G361 cells were transfected with increasing concentrations of p300 expression plasmids, a constant concentration of LKB1 as indicated; ERα, ERE-luc, and pRL-tk or V; left untreated or treated with E2 for 24 h followed by reporter gene assays. Results are two experiments in triplicate. Data are mean ± SD (B) G361 cells were transfected with pRL-tk, ERα, ERE-luc, and increasing concentrations of p53, or LKB1 expression plasmids, left untreated or treated with E2 for 24 h followed by reporter gene assays. Data representative of three experiments in triplicate. Data are mean ± SD. (C) Transfection conditions similar to B; however, in the presence of increasing concentrations of BRCA1. Data are representative of three experiments in triplicate mean ± SD.

Article Snippet: Briefly, MCF7 or G361 cells were transfected with the following plasmids: LKB1 (50 ng), D194A/R304W (50 ng) LKB1 NR mutants (50 ng), pRL-tk (0.5 ng), ERE-luc (gift from Dr. Myles Brown, Harvard University, Boston, MA)/cyclin D1-luc (50 ng), ERα/ERβ (Addgene plasmid 11356) (10 ng)/androgen receptor (AR; 10 ng), glucocorticoid receptor (GR; 50 ng), mouse mammary tumor virus luciferase (MMTV-luc; 50 ng) (gifts from Dr. Steven Balk, Harvard University), p300 (50 ng) (Addgene plasmid 10718; Addgene, Cambridge, MA), BRCA1, and p53 (indicated concentrations).

Techniques: Activity Assay, Transfection, Expressing, Plasmid Preparation, Concentration Assay

LKB1 does not alter ERβ, GR, or AR activity. (A) G361 cells were transfected with LKB1, ERβ, ERE-luc, and pRL-tk expression plasmids or vector (V). Cells were left untreated or treated with E2 (10 nM) for 24 h before harvesting for reporter assay. (B) Cells were transfected with LKB1, AR, MMTV-luc, and pRL-tk expression plasmids or V, followed by testosterone (T; 100 nM) treatment. (C) Cells were transfected with LKB1, GR, MMTV-luc, and pRL-tk expression plasmids or V followed by treatment with dexamethasone (Dex; 100 nM). Results are three experiments in triplicate mean ± SEM.

Journal:

Article Title: LKB1 Catalytic Activity Contributes to Estrogen Receptor ? Signaling

doi: 10.1091/mbc.E08-11-1138

Figure Lengend Snippet: LKB1 does not alter ERβ, GR, or AR activity. (A) G361 cells were transfected with LKB1, ERβ, ERE-luc, and pRL-tk expression plasmids or vector (V). Cells were left untreated or treated with E2 (10 nM) for 24 h before harvesting for reporter assay. (B) Cells were transfected with LKB1, AR, MMTV-luc, and pRL-tk expression plasmids or V, followed by testosterone (T; 100 nM) treatment. (C) Cells were transfected with LKB1, GR, MMTV-luc, and pRL-tk expression plasmids or V followed by treatment with dexamethasone (Dex; 100 nM). Results are three experiments in triplicate mean ± SEM.

Article Snippet: Briefly, MCF7 or G361 cells were transfected with the following plasmids: LKB1 (50 ng), D194A/R304W (50 ng) LKB1 NR mutants (50 ng), pRL-tk (0.5 ng), ERE-luc (gift from Dr. Myles Brown, Harvard University, Boston, MA)/cyclin D1-luc (50 ng), ERα/ERβ (Addgene plasmid 11356) (10 ng)/androgen receptor (AR; 10 ng), glucocorticoid receptor (GR; 50 ng), mouse mammary tumor virus luciferase (MMTV-luc; 50 ng) (gifts from Dr. Steven Balk, Harvard University), p300 (50 ng) (Addgene plasmid 10718; Addgene, Cambridge, MA), BRCA1, and p53 (indicated concentrations).

Techniques: Activity Assay, Transfection, Expressing, Plasmid Preparation, Reporter Assay

LKB1 catalytic activity is necessary for ERα transactivation. (A and B) G361 cells were transfected with LKB1, D194A, R304W, ERα, ERE-luc, and pRL-tk expression plasmids or vector (V), left untreated or treated with E2 or 4-OHT for 24 h before harvesting for reporter assay. Results are representative of two experiments in triplicate. Data are mean ± SD.

Journal:

Article Title: LKB1 Catalytic Activity Contributes to Estrogen Receptor ? Signaling

doi: 10.1091/mbc.E08-11-1138

Figure Lengend Snippet: LKB1 catalytic activity is necessary for ERα transactivation. (A and B) G361 cells were transfected with LKB1, D194A, R304W, ERα, ERE-luc, and pRL-tk expression plasmids or vector (V), left untreated or treated with E2 or 4-OHT for 24 h before harvesting for reporter assay. Results are representative of two experiments in triplicate. Data are mean ± SD.

Article Snippet: Briefly, MCF7 or G361 cells were transfected with the following plasmids: LKB1 (50 ng), D194A/R304W (50 ng) LKB1 NR mutants (50 ng), pRL-tk (0.5 ng), ERE-luc (gift from Dr. Myles Brown, Harvard University, Boston, MA)/cyclin D1-luc (50 ng), ERα/ERβ (Addgene plasmid 11356) (10 ng)/androgen receptor (AR; 10 ng), glucocorticoid receptor (GR; 50 ng), mouse mammary tumor virus luciferase (MMTV-luc; 50 ng) (gifts from Dr. Steven Balk, Harvard University), p300 (50 ng) (Addgene plasmid 10718; Addgene, Cambridge, MA), BRCA1, and p53 (indicated concentrations).

Techniques: Activity Assay, Transfection, Expressing, Plasmid Preparation, Reporter Assay