Journal:

Article Title: Detection of Unculturable Bacteria in Periodontal Health and Disease by PCR

doi:

Figure Lengend Snippet: Specificities of selected reverse primers, used in conjunction with 27F, against the wide-range panel

Article Snippet: Specificities of the PUS9.170 reverse primers, used in conjunction with 27F, against the Eubacterium panel table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Target strain Specificity a PUS3.42 PUS9.180 PUS9.170 131R 590R 202R 483R 845R 1287R 189R 835R Actinobacillus actinomycetemcomitans Y4 * − − − − − − − Agrobacterium tumefaciens GMI G023 * − − − * − − − Actinomyces viscosus NCTC 10951 − − − − − − − − Clostridium perfringens W1678 * − − * * − − − Campylobacter rectus ATCC 33238 − * − − * − − − Eikenella corrodens P5-22 − − − − * − − − Eubacterium brachy ATCC 33089 * * − * * * − − Escherichia coli NCTC 10418 − − − * − − − − Eubacterium infirmum W1471 − − − − * * − − Eubacterium nodatum ATCC 33099 − − − − * − − − Fusobacterium nucleatum NCTC 10562 − − − * * − − − Lactobacillus casei W2005 − − − − * * * − Peptostreptococcus anaerobius NCTC 11460 − − − * * − − − Porphyromonas gingivalis ATCC 33277 * − − * − − − − Peptostreptococcus micros ATCC 33270 − − − − * * − − Prevotella nigrescens NCTC 9336 − − − − − − − − Staphylococcus aureus NCTC 6715 * − − − * * − − Streptococcus mutans NCTC 10832 − − − * * * − − Veillonella parvula NCTC 11463 − − − − * − − − Open in a separate window a +, PCR product of predicted size; −, no PCR product; *, PCR product of size other than that predicted.

Techniques:

Journal: Biomolecules

Article Title: Accelerated Metabolite Levels of Aerobic Glycolysis and the Pentose Phosphate Pathway Are Required for Efficient Replication of Infectious Spleen and Kidney Necrosis Virus in Chinese Perch Brain Cells

doi: 10.3390/biom9090440

Figure Lengend Snippet: The tricarboxylic acid (TCA) cycle was not necessary for ISKNV multiplication. ( A ) Overview of cellular metabolism with glucose. DCA inhibited the expression of PDK to promote the expression of PDH, which promoted pyruvate into the TCA cycle. ( B ) CPB cells were cultured in a complete medium treated with 0–10 μM of DCA, and the cell viability was detected by MTS assay at 72 hpi. ( C ) Real-time quantitation of PDK expression in CPB cells. ( D ) Western blot analysis of PDHx expression in CPB cells. ( E , F ) The glucose consumption and lactate production in ISKNV infected CPB cells. ( G , H ) CPB cells treated with DCA, or without DCA were infected with ISKNV. Viral yield in cells and in supernatant was determined by real-time PCR. * p < 0.05; ** p < 0.01.

Article Snippet: The rabbit polyclonal antibodies against glucose-6-phosphate dehydrogenase (G6PDH), hexokinase 1 (HK1), glucose-6-phosphate isomerase (GPI), enolase (ENO), pyruvate kinase (PK), lactate dehydrogenase (LDH), and pyruvate dehydrogenase complex (PDHx), as well as the mouse monoclonal antibody against β-actin, were purchased from Proteintech (USA).

Techniques: Expressing, Cell Culture, MTS Assay, Quantitation Assay, Western Blot, Infection, Real-time Polymerase Chain Reaction