Journal: Journal of Cellular and Molecular Medicine

Article Title: Syncytiotrophoblast‐derived extracellular vesicles carry apolipoprotein‐E and affect lipid synthesis of liver cells in vitro

doi: 10.1111/jcmm.17056

Figure Lengend Snippet: Expression of ApoE in the human placenta and STBEVs derived from normal pregnancy. (A) Representative immunofluorescence analysis demonstrating (i) apoE expression (red) on the syncytiotrophoblast layer of placental chorionic villi. Cell nuclei staining has been performed with DAPI (blue); (ii) negative control with anti‐ IgG rabbit antibody (n = 6). (B) Representative immunoblot demonstrating apoE (36 KDa) and PLAP (60 KDa) expression on medium/large and small STBEVs derived from normal placentae (P1‐4) (n = 4). *Empty wells with sample overflowed from neighbouring wells. (C) Representative immunoblot showing lack of expression of apo‐A1 (28KDa) on the STBEVs derived from normal placentae (P1‐5) (n = 5). Plasma derived from NP patient at term was used as positive control for lipoproteins ApoE and Apo‐A1. P: placental lysate; STBEVs: syncytiotrophoblast‐derived extracellular vesicles; medium/large STBEVs: STBEV isolated at 10,000xg centrifugation; small STBEVs, STBEV isolated at 150,000xg centrifugation; apoE, apolipoprotein‐E; apo‐A1, apolipoprotein A1

Article Snippet: Permeabilized HepG2 cells were resuspended in PBS and blocked with 0.2 mm filtered Fc receptor blocker (10 µL; Miltenyi) for 10 min before incubation for 15 minutes of either filtered PBS (unlabelled control), NDOG2‐PE‐conjugated antibody or its IgG1‐PE‐conjugated isotype control.

Techniques: Expressing, Derivative Assay, Immunofluorescence, Staining, Negative Control, Western Blot, Positive Control, Isolation, Centrifugation