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  • 93
    MedChemExpress shp 2 inhibitor shp 099
    Hypoxia activates SHP-1 and <t>SHP-2</t> in NK cells. (a–c) Western blotting analysis shows SHP-1 and SHP-2 expression in normoxic (20% O 2 ) and hypoxic (1% O 2 ) KHYG-1 (a), NK92 (b) cells, and primary NK cells (c), respectively. (d) Western blotting analysis shows the effects of SHP-1 inhibitor TPI-1 on the phosphorylation of ERK and STAT3. Hypoxic KYHG-1 cells were pretreated with 5 μ M TPI-1 for 2 h, and then the cells were collected for Western blotting analysis. Representative Western blot images are shown in upper panel; the densitometric analysis is shown in lower panel ( n = 3, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). (e) Flow cytometric analysis of the effects of TPI-1 on the NK cell cytotoxicity. Left panel: representative flow cytometry results of TPI-1 on the cytotoxicity of KHYG-1 cells. KHYG-1 cells were pretreated with 5 μ M TPI-1 for 2 h and then incubated with K562 cells at different E : T ratios for 4 h. Right panel: statistical analysis of the effects of TPI-1 on KHYG-1 cell cytotoxicity against K562 cells ( n = 3, ∗ P < 0.05).
    Shp 2 Inhibitor Shp 099, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shp 2 inhibitor shp 099/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shp 2 inhibitor shp 099 - by Bioz Stars, 2024-02
    93/100 stars
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    93
    Santa Cruz Biotechnology irak2
    Hypoxia activates SHP-1 and <t>SHP-2</t> in NK cells. (a–c) Western blotting analysis shows SHP-1 and SHP-2 expression in normoxic (20% O 2 ) and hypoxic (1% O 2 ) KHYG-1 (a), NK92 (b) cells, and primary NK cells (c), respectively. (d) Western blotting analysis shows the effects of SHP-1 inhibitor TPI-1 on the phosphorylation of ERK and STAT3. Hypoxic KYHG-1 cells were pretreated with 5 μ M TPI-1 for 2 h, and then the cells were collected for Western blotting analysis. Representative Western blot images are shown in upper panel; the densitometric analysis is shown in lower panel ( n = 3, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). (e) Flow cytometric analysis of the effects of TPI-1 on the NK cell cytotoxicity. Left panel: representative flow cytometry results of TPI-1 on the cytotoxicity of KHYG-1 cells. KHYG-1 cells were pretreated with 5 μ M TPI-1 for 2 h and then incubated with K562 cells at different E : T ratios for 4 h. Right panel: statistical analysis of the effects of TPI-1 on KHYG-1 cell cytotoxicity against K562 cells ( n = 3, ∗ P < 0.05).
    Irak2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/irak2/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    irak2 - by Bioz Stars, 2024-02
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    86
    DuPont de Nemours 100388 e i du pont de nemours co
    Hypoxia activates SHP-1 and <t>SHP-2</t> in NK cells. (a–c) Western blotting analysis shows SHP-1 and SHP-2 expression in normoxic (20% O 2 ) and hypoxic (1% O 2 ) KHYG-1 (a), NK92 (b) cells, and primary NK cells (c), respectively. (d) Western blotting analysis shows the effects of SHP-1 inhibitor TPI-1 on the phosphorylation of ERK and STAT3. Hypoxic KYHG-1 cells were pretreated with 5 μ M TPI-1 for 2 h, and then the cells were collected for Western blotting analysis. Representative Western blot images are shown in upper panel; the densitometric analysis is shown in lower panel ( n = 3, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). (e) Flow cytometric analysis of the effects of TPI-1 on the NK cell cytotoxicity. Left panel: representative flow cytometry results of TPI-1 on the cytotoxicity of KHYG-1 cells. KHYG-1 cells were pretreated with 5 μ M TPI-1 for 2 h and then incubated with K562 cells at different E : T ratios for 4 h. Right panel: statistical analysis of the effects of TPI-1 on KHYG-1 cell cytotoxicity against K562 cells ( n = 3, ∗ P < 0.05).
    100388 E I Du Pont De Nemours Co, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100388 e i du pont de nemours co/product/DuPont de Nemours
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    100388 e i du pont de nemours co - by Bioz Stars, 2024-02
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    86
    Bioresource Technology Inc reports 9 2020 100388
    Hypoxia activates SHP-1 and <t>SHP-2</t> in NK cells. (a–c) Western blotting analysis shows SHP-1 and SHP-2 expression in normoxic (20% O 2 ) and hypoxic (1% O 2 ) KHYG-1 (a), NK92 (b) cells, and primary NK cells (c), respectively. (d) Western blotting analysis shows the effects of SHP-1 inhibitor TPI-1 on the phosphorylation of ERK and STAT3. Hypoxic KYHG-1 cells were pretreated with 5 μ M TPI-1 for 2 h, and then the cells were collected for Western blotting analysis. Representative Western blot images are shown in upper panel; the densitometric analysis is shown in lower panel ( n = 3, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). (e) Flow cytometric analysis of the effects of TPI-1 on the NK cell cytotoxicity. Left panel: representative flow cytometry results of TPI-1 on the cytotoxicity of KHYG-1 cells. KHYG-1 cells were pretreated with 5 μ M TPI-1 for 2 h and then incubated with K562 cells at different E : T ratios for 4 h. Right panel: statistical analysis of the effects of TPI-1 on KHYG-1 cell cytotoxicity against K562 cells ( n = 3, ∗ P < 0.05).
    Reports 9 2020 100388, supplied by Bioresource Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reports 9 2020 100388/product/Bioresource Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reports 9 2020 100388 - by Bioz Stars, 2024-02
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    Standard format Plasmid sent in bacteria as agar stab
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    Image Search Results


    Hypoxia activates SHP-1 and SHP-2 in NK cells. (a–c) Western blotting analysis shows SHP-1 and SHP-2 expression in normoxic (20% O 2 ) and hypoxic (1% O 2 ) KHYG-1 (a), NK92 (b) cells, and primary NK cells (c), respectively. (d) Western blotting analysis shows the effects of SHP-1 inhibitor TPI-1 on the phosphorylation of ERK and STAT3. Hypoxic KYHG-1 cells were pretreated with 5 μ M TPI-1 for 2 h, and then the cells were collected for Western blotting analysis. Representative Western blot images are shown in upper panel; the densitometric analysis is shown in lower panel ( n = 3, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). (e) Flow cytometric analysis of the effects of TPI-1 on the NK cell cytotoxicity. Left panel: representative flow cytometry results of TPI-1 on the cytotoxicity of KHYG-1 cells. KHYG-1 cells were pretreated with 5 μ M TPI-1 for 2 h and then incubated with K562 cells at different E : T ratios for 4 h. Right panel: statistical analysis of the effects of TPI-1 on KHYG-1 cell cytotoxicity against K562 cells ( n = 3, ∗ P < 0.05).

    Journal: Journal of Immunology Research

    Article Title: Hypoxia Impairs NK Cell Cytotoxicity through SHP-1-Mediated Attenuation of STAT3 and ERK Signaling Pathways

    doi: 10.1155/2020/4598476

    Figure Lengend Snippet: Hypoxia activates SHP-1 and SHP-2 in NK cells. (a–c) Western blotting analysis shows SHP-1 and SHP-2 expression in normoxic (20% O 2 ) and hypoxic (1% O 2 ) KHYG-1 (a), NK92 (b) cells, and primary NK cells (c), respectively. (d) Western blotting analysis shows the effects of SHP-1 inhibitor TPI-1 on the phosphorylation of ERK and STAT3. Hypoxic KYHG-1 cells were pretreated with 5 μ M TPI-1 for 2 h, and then the cells were collected for Western blotting analysis. Representative Western blot images are shown in upper panel; the densitometric analysis is shown in lower panel ( n = 3, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). (e) Flow cytometric analysis of the effects of TPI-1 on the NK cell cytotoxicity. Left panel: representative flow cytometry results of TPI-1 on the cytotoxicity of KHYG-1 cells. KHYG-1 cells were pretreated with 5 μ M TPI-1 for 2 h and then incubated with K562 cells at different E : T ratios for 4 h. Right panel: statistical analysis of the effects of TPI-1 on KHYG-1 cell cytotoxicity against K562 cells ( n = 3, ∗ P < 0.05).

    Article Snippet: SHP-1 inhibitor TPI-1 (#HY-100463), SHP-2 inhibitor SHP-099 (#HY-100388), and ERK inhibitor U0126 (#HY-12031) were bought from MedChemExpress (Monmouth Junction, New Jersey, USA).

    Techniques: Western Blot, Expressing, Flow Cytometry, Incubation

    Inhibition of SHP-2 has no effect on NK cell cytotoxicity. (a) Western blotting shows the effects of SHP-2 inhibitor SHP099 on the phosphorylation of ERK and STAT3. Hypoxic KYHG-1 cells were pretreated with 5 μ M SHP099 for 2 h, and then the cells were collected for Western blotting analysis. Representative Western blot images are shown in left panel; the densitometric analysis is shown in right panel ( n = 3, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). (b) Flow cytometric analysis shows the effect of SHP099 on the cytotoxicity of KHYG-1 cells. KHYG-1 cells were pretreated with 5 μ M SHP099 for 2 h, then incubated with K562 cells at different E : T ratios for 4 h. (c) Statistical analysis of the effects of SHP099 on KHYG-1 cell cytotoxicity against K562 cells ( n = 3, ns: no significance).

    Journal: Journal of Immunology Research

    Article Title: Hypoxia Impairs NK Cell Cytotoxicity through SHP-1-Mediated Attenuation of STAT3 and ERK Signaling Pathways

    doi: 10.1155/2020/4598476

    Figure Lengend Snippet: Inhibition of SHP-2 has no effect on NK cell cytotoxicity. (a) Western blotting shows the effects of SHP-2 inhibitor SHP099 on the phosphorylation of ERK and STAT3. Hypoxic KYHG-1 cells were pretreated with 5 μ M SHP099 for 2 h, and then the cells were collected for Western blotting analysis. Representative Western blot images are shown in left panel; the densitometric analysis is shown in right panel ( n = 3, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). (b) Flow cytometric analysis shows the effect of SHP099 on the cytotoxicity of KHYG-1 cells. KHYG-1 cells were pretreated with 5 μ M SHP099 for 2 h, then incubated with K562 cells at different E : T ratios for 4 h. (c) Statistical analysis of the effects of SHP099 on KHYG-1 cell cytotoxicity against K562 cells ( n = 3, ns: no significance).

    Article Snippet: SHP-1 inhibitor TPI-1 (#HY-100463), SHP-2 inhibitor SHP-099 (#HY-100388), and ERK inhibitor U0126 (#HY-12031) were bought from MedChemExpress (Monmouth Junction, New Jersey, USA).

    Techniques: Inhibition, Western Blot, Incubation