Journal: Nature cell biology
Article Title: FBP1 loss disrupts liver metabolism and promotes tumourigenesis through a hepatic stellate cell senescence secretome
Figure Lengend Snippet: Decreased FBP1 expression in human and murine liver tumours (a) Metabolic gene set analysis of TCGA HCC RNA-sequencing data. A total of 374 HCC tumours and 50 adjacent normal tissues were included, and 2752 genes encoding all known human metabolic enzymes and transporters were classified according to the Kyoto Encyclopedia of Genes and Genomes (KEGG). Generated metabolic gene sets were ranked based on their median fold expression changes in HCC tumours vs normal tissue, and plotted as median ± median absolute deviation. (b) Representative FBP1 IHC staining on human liver tissue array with adjacent normal, grade 2 and 3 HCC tissues. Scale bar: 100 μm. ( c ) Statistical analysis of FBP1 IHC staining in ( b ). n=20 for normal, n=30 for grade 2, n=30 for grade 3 samples. In each box plot, the top-most line is the maximum, the top of the box is the third quartile, the centre line is the median, the bottom of the box is the first quartile and the bottom-most line is the minimum. ( d ) Representative H E staining in liver sections from 24-week control (Ctrl) and DEN-treated (DEN) C57BL/6 mice (n = 3 independent experiments with similar results). T, tumour. Scale bar: 100 μm. (e) Serum alanine transaminase (ALT) activity from 24-week Ctrl and DEN mice. n=4 for Ctrl, n=5 for DEN. Graph in e show mean ± SEM, and P value was calculated using a two-tailed t-test. Numerical source data are provided in Source Data Extended Data Fig. 1.
Article Snippet: Primary mouse HSCs were isolated from 10-week C57BL/6 mice following a previous protocol .
Techniques: Expressing, RNA Sequencing Assay, Generated, Immunohistochemistry, Staining, Mouse Assay, Activity Assay, Two Tailed Test