100 bp paired end mode Illumina Inc Search Results


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  • 94
    Illumina Inc 100 bp paired end mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    100 Bp Paired End Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 bp paired end mode/product/Illumina Inc
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    100 bp paired end mode - by Bioz Stars, 2020-05
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    96
    Illumina Inc paired end mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Paired End Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paired end mode/product/Illumina Inc
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    88
    Illumina Inc end 100 bp mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    End 100 Bp Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end 100 bp mode/product/Illumina Inc
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    88
    Illumina Inc multiplex illumina hiseq high output mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Multiplex Illumina Hiseq High Output Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex illumina hiseq high output mode/product/Illumina Inc
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    93
    Illumina Inc rapid mode illumina hiseq 2500 system
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Rapid Mode Illumina Hiseq 2500 System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid mode illumina hiseq 2500 system/product/Illumina Inc
    Average 93 stars, based on 21 article reviews
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    90
    Illumina Inc rapid mode illumina hiseq2500 sequencing
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Rapid Mode Illumina Hiseq2500 Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid mode illumina hiseq2500 sequencing/product/Illumina Inc
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
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    99
    Illumina Inc hiseq 2500 instrument
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Hiseq 2500 Instrument, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 5355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 instrument/product/Illumina Inc
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    93
    Illumina Inc pe100
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Pe100, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe100/product/Illumina Inc
    Average 93 stars, based on 145 article reviews
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    93
    Illumina Inc truseq rapid pe cluster kit hs
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Truseq Rapid Pe Cluster Kit Hs, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Illumina Inc hiseq 2000 analyzers
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Hiseq 2000 Analyzers, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc hiseq 2000 sequencing system
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Hiseq 2000 Sequencing System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 2255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 sequencing system/product/Illumina Inc
    Average 99 stars, based on 2255 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 sequencing system - by Bioz Stars, 2020-05
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    Image Search Results


    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, 100 μm wide field). Error bars, SEM; *p

    Journal: eLife

    Article Title: Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development

    doi: 10.7554/eLife.18683

    Figure Lengend Snippet: Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, 100 μm wide field). Error bars, SEM; *p

    Article Snippet: Up to 192 cells were pooled and sequenced in 100-bp paired-end mode on one lane of an Illumina HiSeq 2500 platform (rapid mode).

    Techniques: Staining

    Cell cycle assignment for differential gene expression analysis. ( A ) Hierarchical clustering was used to identify human organoid APs that most strongly expressed genes enriched in G2M phase of the cell cycle (red). The genes were identified from PCA on fetal cortex progenitor cells (top 100 correlating genes) ( Camp et al., 2015 ). The cluster with weakest expression of these G2M associated genes was assigned as G1 phase (blue). Intermediate cells (grey) were discarded from differential gene expression analysis. ( B ) A previously published method was used to computationally assign cell-cycle stage based on a machine-learning approach ( Scialdone et al., 2015 ). This method was generally consistent with our assignment based on the hierarchical clustering presented in panel A . ( C – F ) The same approach was used to identify the chimpanzee organoid APs, endothelial cells (ECs), and iPSCs that most highly express G2M markers. Note that all iPSCs analyzed highly expressed most of the G2M marker genes. DOI: http://dx.doi.org/10.7554/eLife.18683.026

    Journal: eLife

    Article Title: Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development

    doi: 10.7554/eLife.18683

    Figure Lengend Snippet: Cell cycle assignment for differential gene expression analysis. ( A ) Hierarchical clustering was used to identify human organoid APs that most strongly expressed genes enriched in G2M phase of the cell cycle (red). The genes were identified from PCA on fetal cortex progenitor cells (top 100 correlating genes) ( Camp et al., 2015 ). The cluster with weakest expression of these G2M associated genes was assigned as G1 phase (blue). Intermediate cells (grey) were discarded from differential gene expression analysis. ( B ) A previously published method was used to computationally assign cell-cycle stage based on a machine-learning approach ( Scialdone et al., 2015 ). This method was generally consistent with our assignment based on the hierarchical clustering presented in panel A . ( C – F ) The same approach was used to identify the chimpanzee organoid APs, endothelial cells (ECs), and iPSCs that most highly express G2M markers. Note that all iPSCs analyzed highly expressed most of the G2M marker genes. DOI: http://dx.doi.org/10.7554/eLife.18683.026

    Article Snippet: Up to 192 cells were pooled and sequenced in 100-bp paired-end mode on one lane of an Illumina HiSeq 2500 platform (rapid mode).

    Techniques: Expressing, Marker