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  • 96
    DSMZ type e coli mg1655
    Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli <t>MG1655</t> and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
    Type E Coli Mg1655, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    DSMZ formosa agariphila kmm 3901
    Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli <t>MG1655</t> and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
    Formosa Agariphila Kmm 3901, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    DSMZ brachyspira hyodysenteriae dsm105803
    Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli <t>MG1655</t> and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
    Brachyspira Hyodysenteriae Dsm105803, supplied by DSMZ, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    DSMZ dsm15637
    Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli <t>MG1655</t> and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
    Dsm15637, supplied by DSMZ, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    DSMZ dsm108250
    Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli <t>MG1655</t> and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
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    Image Search Results


    Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli MG1655 and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

    Journal: ACS Synthetic Biology

    Article Title: A Transcription Factor-Based Biosensor for Detection of Itaconic Acid

    doi: 10.1021/acssynbio.8b00057

    Figure Lengend Snippet: Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli MG1655 and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

    Article Snippet: RFP fluorescence assays for biosensor characterization were performed in wild type E. coli MG1655 (DSMZ 18039) and C. necator H16 (ATCC 17699).

    Techniques: Expressing, Fluorescence, Plasmid Preparation, Negative Control

    Kinetics and dynamics of the Yp ItcR/P ccl inducible system. (A) Absolute normalized fluorescence of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system (pEH086) in response to different concentrations of itaconate added at time zero. The standard deviation of three biological replicates is shown as a lighter color ribbon displayed lengthwise of the induction kinetics curve. For the lower concentrations, the standard deviation is too small to be visible. (B) Dose response curve of the Yp ItcR/P ccl inducible system in E. coli MG1655, illustrating the correlation between inducer concentration and fluorescence output 4 and 8 h post-induction (hpi) with itaconate. Error bars represent standard deviations of three biological replicates.

    Journal: ACS Synthetic Biology

    Article Title: A Transcription Factor-Based Biosensor for Detection of Itaconic Acid

    doi: 10.1021/acssynbio.8b00057

    Figure Lengend Snippet: Kinetics and dynamics of the Yp ItcR/P ccl inducible system. (A) Absolute normalized fluorescence of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system (pEH086) in response to different concentrations of itaconate added at time zero. The standard deviation of three biological replicates is shown as a lighter color ribbon displayed lengthwise of the induction kinetics curve. For the lower concentrations, the standard deviation is too small to be visible. (B) Dose response curve of the Yp ItcR/P ccl inducible system in E. coli MG1655, illustrating the correlation between inducer concentration and fluorescence output 4 and 8 h post-induction (hpi) with itaconate. Error bars represent standard deviations of three biological replicates.

    Article Snippet: RFP fluorescence assays for biosensor characterization were performed in wild type E. coli MG1655 (DSMZ 18039) and C. necator H16 (ATCC 17699).

    Techniques: Fluorescence, Standard Deviation, Concentration Assay

    Inducer-dependent orthogonality of the Yp ItcR/P ccl inducible system. (A) Compounds that were investigated for cross-induction with the Yp ItcR/P ccl inducible system: itaconic acid (1), succinic acid (2), d -malic acid (3), l -malic acid (4), fumaric acid (5), oxaloacetic acid (6), l -aspartic acid (7), methylsuccinic acid (8), mesaconic acid (9), citraconic acid (10), α-ketoglutaric acid (11), l -glutamic acid (12), acetic acid (13), propionic acid (14), butyric acid (15), 3-butenoic acid (16), valeric acid (17), acrylic acid (18), methacrylic acid (19), tiglic acid (20), citric acid (21), cis -aconitic acid (22), trans -aconitic acid (23), tricarballylic acid (24), isocitric acid (25). (B) Normalized fluorescence (in %) of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system 12 hours after addition of different compounds at a final concentration of 5 mM, relative to the fluorescence output obtained by adding 5 mM itaconate. (−) uninduced sample. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

    Journal: ACS Synthetic Biology

    Article Title: A Transcription Factor-Based Biosensor for Detection of Itaconic Acid

    doi: 10.1021/acssynbio.8b00057

    Figure Lengend Snippet: Inducer-dependent orthogonality of the Yp ItcR/P ccl inducible system. (A) Compounds that were investigated for cross-induction with the Yp ItcR/P ccl inducible system: itaconic acid (1), succinic acid (2), d -malic acid (3), l -malic acid (4), fumaric acid (5), oxaloacetic acid (6), l -aspartic acid (7), methylsuccinic acid (8), mesaconic acid (9), citraconic acid (10), α-ketoglutaric acid (11), l -glutamic acid (12), acetic acid (13), propionic acid (14), butyric acid (15), 3-butenoic acid (16), valeric acid (17), acrylic acid (18), methacrylic acid (19), tiglic acid (20), citric acid (21), cis -aconitic acid (22), trans -aconitic acid (23), tricarballylic acid (24), isocitric acid (25). (B) Normalized fluorescence (in %) of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system 12 hours after addition of different compounds at a final concentration of 5 mM, relative to the fluorescence output obtained by adding 5 mM itaconate. (−) uninduced sample. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

    Article Snippet: RFP fluorescence assays for biosensor characterization were performed in wild type E. coli MG1655 (DSMZ 18039) and C. necator H16 (ATCC 17699).

    Techniques: Fluorescence, Concentration Assay