1 1000 anti cd81 Search Results


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    • anti cd63  (Millipore)
    86
    Millipore anti cd63
    Anti Cd63, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd63/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd63 - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier
    anti cd81  (Abcam)
    86
    Abcam anti cd81
    Anti Cd81, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd81/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd81 - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier
    anti cd81  (ABclonal Biotechnology)
    86
    ABclonal Biotechnology anti cd81
    ACKR1 is secreted in extracellular vesicles after removal of blood. HPMECs were incubated with blood or media for 24 hours and subsequently washed and incubated with serum-free media for 6 hours. (A, B) Extracellular vesicles (EVs) in the supernatant at 6 hours after blood removal were isolated by size-exclusion chromatography; ACKR1 (using the NBP2-75197 antibody) and EV markers CD63 and <t>CD81</t> were detected by immunoblotting. (C) Cells were treated with the EV inhibitor GW4869 (2 μM) after blood removal for 6 hours. Blot shows whole cell lysate at 0 and 6 hours after blood removal with or without the inhibitor. (D) Quantification of ACKR1 levels in cell lysates (****p<0.0001; *p<0.05).
    Anti Cd81, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd81/product/ABclonal Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd81 - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    ACKR1 is secreted in extracellular vesicles after removal of blood. HPMECs were incubated with blood or media for 24 hours and subsequently washed and incubated with serum-free media for 6 hours. (A, B) Extracellular vesicles (EVs) in the supernatant at 6 hours after blood removal were isolated by size-exclusion chromatography; ACKR1 (using the NBP2-75197 antibody) and EV markers CD63 and CD81 were detected by immunoblotting. (C) Cells were treated with the EV inhibitor GW4869 (2 μM) after blood removal for 6 hours. Blot shows whole cell lysate at 0 and 6 hours after blood removal with or without the inhibitor. (D) Quantification of ACKR1 levels in cell lysates (****p<0.0001; *p<0.05).

    Journal: Frontiers in Immunology

    Article Title: Endothelial ACKR1 is induced by neutrophil contact and down-regulated by secretion in extracellular vesicles

    doi: 10.3389/fimmu.2023.1181016

    Figure Lengend Snippet: ACKR1 is secreted in extracellular vesicles after removal of blood. HPMECs were incubated with blood or media for 24 hours and subsequently washed and incubated with serum-free media for 6 hours. (A, B) Extracellular vesicles (EVs) in the supernatant at 6 hours after blood removal were isolated by size-exclusion chromatography; ACKR1 (using the NBP2-75197 antibody) and EV markers CD63 and CD81 were detected by immunoblotting. (C) Cells were treated with the EV inhibitor GW4869 (2 μM) after blood removal for 6 hours. Blot shows whole cell lysate at 0 and 6 hours after blood removal with or without the inhibitor. (D) Quantification of ACKR1 levels in cell lysates (****p<0.0001; *p<0.05).

    Article Snippet: Primary antibodies utilized were anti-CD63 (ABclonal A5271, 1:1000) and anti-CD81 (ABclonal A5270, 1:1000).

    Techniques: Incubation, Isolation, Size-exclusion Chromatography, Western Blot