0856009 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Valiant rabbit anti β galactosidase
    The pro-apoptotic activation of p53 is enhanced by nonselective HDACis. a, b Neurons were treated with etoposide in the presence or absence of the pan-HDACis for 6 h as indicated (vehicle, 0.2 % DMSO, V; 0.1 μM TSA, TS; 10 μM SBHA, SB; 10 μM SAHA, SA). Levels of the Ser15-phosphorylated (P-p53) p53 and the total p53 (T-p53) were determined by immunoblotting; the membranes were reprobed for β-actin to ensure equal protein loading in each lane . a Blots from representative experiments; numbers under the blots indicate optical density ratios of the bands for the P-p53 and the β-actin or the T-p53 and the β-actin (fold control). b Quantification of phospho-p53 levels as determined in three independent experiments. Pan-HDACis increased p53 activation in response to etopo-side. In addition, TSA or SBHA weakly increased p53 phosphorylation/accumulation without etoposide. c Apoptosis in response to the overexpressed p53 is enhanced by the pan-HDACi TSA. Neurons were co-transfected with expression plasmids for <t>β-gal</t> and either a temperature-sensitive mutant of p53 (TS-p53) or an empty expression vector (pCMV, vector; 0.2 + 0.1 μg of plasmid DNAs/5 × 10 5 neurons, respectively). For next 24 h, neurons were kept at 37 °C. At this temperature, TS-p53 remained in an inactive conformation. Then, neurons were placed at 32 °C restoring wild-type p53 conformation of TS-p53. In addition, cells were treated with TSA as indicated. At 24 h after the temperature shift, TS-p53 induced apoptosis in TSA-treated neurons. Representative micrographs of transfected (i.e., β-gal-positive) neurons are shown; a surviving neuron with uniformly stained nucleus is indicated by an arrow ; an arrowhead identifies an apoptotic neuron with fragmentation of nuclear chromatin. The graph presents quantitation of the apoptosis response from three independent experiments (2 sister cultures/ experiment/condition). In b, c , error bars represent SD; NS p > 0.05; * p
    Rabbit Anti β Galactosidase, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti β galactosidase/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti β galactosidase - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

    93
    Valiant anti β galactosidase
    β‐Catenin and pGSK‐3β proteins levels normalized to <t>β‐Actin</t> (40 KD) after 8 hrs of incubation in NTM cells following exposure to different concentrations of ODM and RPE EVs. Total protein was extracted from NTM cells and analysed by Western blot. ( A ) Representative Western blot film. ( B ) A significant increase in β‐Catenin (90 KD) expression in NTM cells following (X10) treatment of NPCE EVs. A significant increase in pGSK‐3β (45 KD) expression in NTM cells following (X10) and (X2) treatments of NPCE EVs. The bar graph represents the means ± S.D from three independent experiments performed in triplicates. One‐way ANOVA was used to determine statistical difference, as indicated by asterisks (* P
    Anti β Galactosidase, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti β galactosidase/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti β galactosidase - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    The pro-apoptotic activation of p53 is enhanced by nonselective HDACis. a, b Neurons were treated with etoposide in the presence or absence of the pan-HDACis for 6 h as indicated (vehicle, 0.2 % DMSO, V; 0.1 μM TSA, TS; 10 μM SBHA, SB; 10 μM SAHA, SA). Levels of the Ser15-phosphorylated (P-p53) p53 and the total p53 (T-p53) were determined by immunoblotting; the membranes were reprobed for β-actin to ensure equal protein loading in each lane . a Blots from representative experiments; numbers under the blots indicate optical density ratios of the bands for the P-p53 and the β-actin or the T-p53 and the β-actin (fold control). b Quantification of phospho-p53 levels as determined in three independent experiments. Pan-HDACis increased p53 activation in response to etopo-side. In addition, TSA or SBHA weakly increased p53 phosphorylation/accumulation without etoposide. c Apoptosis in response to the overexpressed p53 is enhanced by the pan-HDACi TSA. Neurons were co-transfected with expression plasmids for β-gal and either a temperature-sensitive mutant of p53 (TS-p53) or an empty expression vector (pCMV, vector; 0.2 + 0.1 μg of plasmid DNAs/5 × 10 5 neurons, respectively). For next 24 h, neurons were kept at 37 °C. At this temperature, TS-p53 remained in an inactive conformation. Then, neurons were placed at 32 °C restoring wild-type p53 conformation of TS-p53. In addition, cells were treated with TSA as indicated. At 24 h after the temperature shift, TS-p53 induced apoptosis in TSA-treated neurons. Representative micrographs of transfected (i.e., β-gal-positive) neurons are shown; a surviving neuron with uniformly stained nucleus is indicated by an arrow ; an arrowhead identifies an apoptotic neuron with fragmentation of nuclear chromatin. The graph presents quantitation of the apoptosis response from three independent experiments (2 sister cultures/ experiment/condition). In b, c , error bars represent SD; NS p > 0.05; * p

    Journal: Neuromolecular medicine

    Article Title: Inhibitors of Histone Deacetylases Enhance Neurotoxicity of DNA Damage

    doi: 10.1007/s12017-014-8322-x

    Figure Lengend Snippet: The pro-apoptotic activation of p53 is enhanced by nonselective HDACis. a, b Neurons were treated with etoposide in the presence or absence of the pan-HDACis for 6 h as indicated (vehicle, 0.2 % DMSO, V; 0.1 μM TSA, TS; 10 μM SBHA, SB; 10 μM SAHA, SA). Levels of the Ser15-phosphorylated (P-p53) p53 and the total p53 (T-p53) were determined by immunoblotting; the membranes were reprobed for β-actin to ensure equal protein loading in each lane . a Blots from representative experiments; numbers under the blots indicate optical density ratios of the bands for the P-p53 and the β-actin or the T-p53 and the β-actin (fold control). b Quantification of phospho-p53 levels as determined in three independent experiments. Pan-HDACis increased p53 activation in response to etopo-side. In addition, TSA or SBHA weakly increased p53 phosphorylation/accumulation without etoposide. c Apoptosis in response to the overexpressed p53 is enhanced by the pan-HDACi TSA. Neurons were co-transfected with expression plasmids for β-gal and either a temperature-sensitive mutant of p53 (TS-p53) or an empty expression vector (pCMV, vector; 0.2 + 0.1 μg of plasmid DNAs/5 × 10 5 neurons, respectively). For next 24 h, neurons were kept at 37 °C. At this temperature, TS-p53 remained in an inactive conformation. Then, neurons were placed at 32 °C restoring wild-type p53 conformation of TS-p53. In addition, cells were treated with TSA as indicated. At 24 h after the temperature shift, TS-p53 induced apoptosis in TSA-treated neurons. Representative micrographs of transfected (i.e., β-gal-positive) neurons are shown; a surviving neuron with uniformly stained nucleus is indicated by an arrow ; an arrowhead identifies an apoptotic neuron with fragmentation of nuclear chromatin. The graph presents quantitation of the apoptosis response from three independent experiments (2 sister cultures/ experiment/condition). In b, c , error bars represent SD; NS p > 0.05; * p

    Article Snippet: Immunofluorescence for β-gal was performed using a rabbit anti-β-gal antibody (MP Biomedicals) as described previously ( ).

    Techniques: Activation Assay, Transfection, Expressing, Mutagenesis, Plasmid Preparation, Staining, Quantitation Assay

    β‐Catenin and pGSK‐3β proteins levels normalized to β‐Actin (40 KD) after 8 hrs of incubation in NTM cells following exposure to different concentrations of ODM and RPE EVs. Total protein was extracted from NTM cells and analysed by Western blot. ( A ) Representative Western blot film. ( B ) A significant increase in β‐Catenin (90 KD) expression in NTM cells following (X10) treatment of NPCE EVs. A significant increase in pGSK‐3β (45 KD) expression in NTM cells following (X10) and (X2) treatments of NPCE EVs. The bar graph represents the means ± S.D from three independent experiments performed in triplicates. One‐way ANOVA was used to determine statistical difference, as indicated by asterisks (* P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Extracellular vesicles have variable dose‐dependent effects on cultured draining cells in the eye

    doi: 10.1111/jcmm.13505

    Figure Lengend Snippet: β‐Catenin and pGSK‐3β proteins levels normalized to β‐Actin (40 KD) after 8 hrs of incubation in NTM cells following exposure to different concentrations of ODM and RPE EVs. Total protein was extracted from NTM cells and analysed by Western blot. ( A ) Representative Western blot film. ( B ) A significant increase in β‐Catenin (90 KD) expression in NTM cells following (X10) treatment of NPCE EVs. A significant increase in pGSK‐3β (45 KD) expression in NTM cells following (X10) and (X2) treatments of NPCE EVs. The bar graph represents the means ± S.D from three independent experiments performed in triplicates. One‐way ANOVA was used to determine statistical difference, as indicated by asterisks (* P

    Article Snippet: Proteins were transferred to nitrocellulose membrane and probed with primary antibodies against β‐Catenin (Cells Signalling Technology, Danvers, MA, USA D10A8 XP R rabbit mAb, c8480s, 1:3000 dilution), pGSK‐3β (Cells Signalling Technology Ser9 5B3, D85E12, XP R rabbit mAb, 1:3000 dilution). β‐Actin was determined using anti‐β‐Actin (MP Biomedicals, Santa Ana, CA, USA, 691001, 1:15,000 dilution).

    Techniques: Incubation, Western Blot, Expressing