Journal: Molecular Medicine Reports
Article Title: Distinct cellular phenotype linked to defective DNA interstrand crosslink repair and homologous recombination
Figure Lengend Snippet: Clonogenic survival of CL-V8B and the parental cell line V79B following exposure to (A) MMC, (B) CDDP, (C) UVC radiation, (D) hydrogen peroxide, (E) CPT, (F) HU, (G) BLM, (H) 6-tG, (I) poly (adenosine diphosphate-ribose) polymerase inhibitor KU0058948. Data are presented as the mean values from at least three independent experiments. Error bars represent standard error. MMC, mitomycin C; CDDP, cisplatin; CPT, camptothecin; HU, hydroxyurea; BLM, bleomycin; 6-tG, 6-tioguanine.
Article Snippet: At the indicated time points (8, 24, 48 and 56 h) after the MMC or BLM treatment, the cells were washed and fixed in 2% formaldehyde (MP Biomedicals LLC, Solon, OH, USA) for 15 min. Then, the cells were permeabilized with 0.1% Triton X-100 (MP Biomedicals LLC) and blocked for 30 min with PBS (PBS+) containing 0.15% glycine and 0.5% bovine serum albumin (Sigma-Aldrich; Merck Millipore).
Techniques: Cycling Probe Technology