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  • 94
    Valiant tamoxifen
    PERK/eIF2α signaling induces autophagy in Xbp1 -deficient intestinal epithelial cells a, b, Immunoblot for LC3 conversion in isolated primary IECs ( a ) ( n =5/4) and for autophagy proteins in primary IEC scrapings ( b ) ( n =3). c, Transmission electron microscopy (TEM) of crypts. Note autophagic vacuoles in various stages of evolution in Xbp1 ΔIEC hypomorphic Paneth cells. d, e, Crypt showing GFP-LC3 punctae ( d ), quantified in ( e ) ( n =10; unpaired Student’s t-test). Bar, 5 μm. f, g, Immunoblot of silenced MODE-K cells ( f ) and primary IEC scrapings ( g ) for the PERK/eIF2α branch ( n =3). h, i, Promoter sequence qPCR for Map1lc3b ( LC3b ) ( h ) and Atg7 ( i ) after anti-ATF4 ChIP (unpaired Student’s t-test). j, GFP-LC3 punctae per crypt after treatment with <t>tamoxifen</t> for 3 days and vehicle or salubrinal ( n= 10; one-way ANOVA with post-hoc Bonferroni). ( k ) Enteritis histology score after salubrinal and tamoxifen co-treatment ( n =12/14/13; median shown; Kruskal-Wallis with post-hoc Holm’s-corrected Mann-Whitney U). Results represent three ( a, f, g ) or two ( c, e, h, i ) independent experiments. * P
    Tamoxifen, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Valiant nile red
    PERK/eIF2α signaling induces autophagy in Xbp1 -deficient intestinal epithelial cells a, b, Immunoblot for LC3 conversion in isolated primary IECs ( a ) ( n =5/4) and for autophagy proteins in primary IEC scrapings ( b ) ( n =3). c, Transmission electron microscopy (TEM) of crypts. Note autophagic vacuoles in various stages of evolution in Xbp1 ΔIEC hypomorphic Paneth cells. d, e, Crypt showing GFP-LC3 punctae ( d ), quantified in ( e ) ( n =10; unpaired Student’s t-test). Bar, 5 μm. f, g, Immunoblot of silenced MODE-K cells ( f ) and primary IEC scrapings ( g ) for the PERK/eIF2α branch ( n =3). h, i, Promoter sequence qPCR for Map1lc3b ( LC3b ) ( h ) and Atg7 ( i ) after anti-ATF4 ChIP (unpaired Student’s t-test). j, GFP-LC3 punctae per crypt after treatment with <t>tamoxifen</t> for 3 days and vehicle or salubrinal ( n= 10; one-way ANOVA with post-hoc Bonferroni). ( k ) Enteritis histology score after salubrinal and tamoxifen co-treatment ( n =12/14/13; median shown; Kruskal-Wallis with post-hoc Holm’s-corrected Mann-Whitney U). Results represent three ( a, f, g ) or two ( c, e, h, i ) independent experiments. * P
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    94
    Valiant cyclophosphamide
    PERK/eIF2α signaling induces autophagy in Xbp1 -deficient intestinal epithelial cells a, b, Immunoblot for LC3 conversion in isolated primary IECs ( a ) ( n =5/4) and for autophagy proteins in primary IEC scrapings ( b ) ( n =3). c, Transmission electron microscopy (TEM) of crypts. Note autophagic vacuoles in various stages of evolution in Xbp1 ΔIEC hypomorphic Paneth cells. d, e, Crypt showing GFP-LC3 punctae ( d ), quantified in ( e ) ( n =10; unpaired Student’s t-test). Bar, 5 μm. f, g, Immunoblot of silenced MODE-K cells ( f ) and primary IEC scrapings ( g ) for the PERK/eIF2α branch ( n =3). h, i, Promoter sequence qPCR for Map1lc3b ( LC3b ) ( h ) and Atg7 ( i ) after anti-ATF4 ChIP (unpaired Student’s t-test). j, GFP-LC3 punctae per crypt after treatment with <t>tamoxifen</t> for 3 days and vehicle or salubrinal ( n= 10; one-way ANOVA with post-hoc Bonferroni). ( k ) Enteritis histology score after salubrinal and tamoxifen co-treatment ( n =12/14/13; median shown; Kruskal-Wallis with post-hoc Holm’s-corrected Mann-Whitney U). Results represent three ( a, f, g ) or two ( c, e, h, i ) independent experiments. * P
    Cyclophosphamide, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PERK/eIF2α signaling induces autophagy in Xbp1 -deficient intestinal epithelial cells a, b, Immunoblot for LC3 conversion in isolated primary IECs ( a ) ( n =5/4) and for autophagy proteins in primary IEC scrapings ( b ) ( n =3). c, Transmission electron microscopy (TEM) of crypts. Note autophagic vacuoles in various stages of evolution in Xbp1 ΔIEC hypomorphic Paneth cells. d, e, Crypt showing GFP-LC3 punctae ( d ), quantified in ( e ) ( n =10; unpaired Student’s t-test). Bar, 5 μm. f, g, Immunoblot of silenced MODE-K cells ( f ) and primary IEC scrapings ( g ) for the PERK/eIF2α branch ( n =3). h, i, Promoter sequence qPCR for Map1lc3b ( LC3b ) ( h ) and Atg7 ( i ) after anti-ATF4 ChIP (unpaired Student’s t-test). j, GFP-LC3 punctae per crypt after treatment with tamoxifen for 3 days and vehicle or salubrinal ( n= 10; one-way ANOVA with post-hoc Bonferroni). ( k ) Enteritis histology score after salubrinal and tamoxifen co-treatment ( n =12/14/13; median shown; Kruskal-Wallis with post-hoc Holm’s-corrected Mann-Whitney U). Results represent three ( a, f, g ) or two ( c, e, h, i ) independent experiments. * P

    Journal: Nature

    Article Title: Paneth cells as a site of origin for intestinal inflammation

    doi: 10.1038/nature12599

    Figure Lengend Snippet: PERK/eIF2α signaling induces autophagy in Xbp1 -deficient intestinal epithelial cells a, b, Immunoblot for LC3 conversion in isolated primary IECs ( a ) ( n =5/4) and for autophagy proteins in primary IEC scrapings ( b ) ( n =3). c, Transmission electron microscopy (TEM) of crypts. Note autophagic vacuoles in various stages of evolution in Xbp1 ΔIEC hypomorphic Paneth cells. d, e, Crypt showing GFP-LC3 punctae ( d ), quantified in ( e ) ( n =10; unpaired Student’s t-test). Bar, 5 μm. f, g, Immunoblot of silenced MODE-K cells ( f ) and primary IEC scrapings ( g ) for the PERK/eIF2α branch ( n =3). h, i, Promoter sequence qPCR for Map1lc3b ( LC3b ) ( h ) and Atg7 ( i ) after anti-ATF4 ChIP (unpaired Student’s t-test). j, GFP-LC3 punctae per crypt after treatment with tamoxifen for 3 days and vehicle or salubrinal ( n= 10; one-way ANOVA with post-hoc Bonferroni). ( k ) Enteritis histology score after salubrinal and tamoxifen co-treatment ( n =12/14/13; median shown; Kruskal-Wallis with post-hoc Holm’s-corrected Mann-Whitney U). Results represent three ( a, f, g ) or two ( c, e, h, i ) independent experiments. * P

    Article Snippet: V-Cre-ERT2+ recombinase was activated by 3 or 5 daily intraperitoneal administrations of 1 mg tamoxifen (MP Biomedicals) as indicated in figure legends.

    Techniques: Isolation, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Sequencing, Real-time Polymerase Chain Reaction, Chromatin Immunoprecipitation, MANN-WHITNEY