Journal: JCI insight

Article Title: Cannabinoid enhancement of lncRNA MMP25-AS1/MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection.

doi: 10.1172/jci.insight.167903

Figure Lengend Snippet: Figure 3. THC decreases MMP25 while elevating TIMP2 protein expression in CE of SIV-infected RMs. Whole colon tissue sections were immunostained for MMP25 (A) (green) and TIMP2 (E) (red) and DAPI for nuclear staining (blue). Note the increased MMP25 (A) and decreased TIMP2 (E) protein expression, specifically in CE of VEH/SIV (n = 5) compared with THC/SIV (n = 5) and control (n = 5) RMs. Representative immunofluorescence images were captured using a Zeiss confocal microscope at 20× original magnification. Scale bars: 50 μm. The demarcation of CE regions excluding the lamina propria compart- ment using the freehand tool in HALO (B). Differences in MMP25 (C) and TIMP2 (F) signal intensity between groups were analyzed using 1-way ANOVA employing the Prism v9 (GraphPad Prism). A P value of <0.05 was considered significant. Data represent mean ± SEM. STRING v 11.0 software was used to predict the MMP25 protein-protein interactions with other proteins (D). Each node represents all the proteins produced by a single protein-coding gene locus. Edges represent protein-protein associations.

Article Snippet: Immunofluorescence studies for the detection of MMP25 (1:50) (Abcam; ab56309), TIMP2 (1:100) (Novus Biologicals; NBP2-01573), CD47 (1:200) (Mybiosource.com; MBS8106360), MPO (1:6,000) (Abcam; A0398), and CD11b (1:100) (Abcam; ab34216) were performed as described previously (16).

Techniques: Expressing, Infection, Staining, Control, Immunofluorescence, Microscopy, Software, Protein-Protein interactions, Produced

Journal: JCI insight

Article Title: Cannabinoid enhancement of lncRNA MMP25-AS1/MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection.

doi: 10.1172/jci.insight.167903

Figure Lengend Snippet: Figure 8. Impact of THC enhancement of MMP25-AS1/MMP25 interaction on neutrophil transendothelial/ transepithelial migration in HIV/SIV infection. At the transcriptional level, MMP25-AS1 directly interacts with MMP25, a matrix metalloproteinase known to enhance the activation of neutrophils and promote inflammation and barrier disruption, allowing microbial translocation. Increased TIMP2 expression results in a further decrease of MMP25 expression at the protein level. The reduction of MMP25 expression also results in a concomitant decrease in CXCL5 and CCL15 expression, 2 potent neutrophil chemoattractants activated by MMP25-mediated cleavage, thus highlighting THC’s potential to block a feed-forward mechanism for neutrophil recruitment. Importantly, THC administration to SIV-infected RMs on long-term cART successfully reduced MMP25 expression in JE of chronically SIV-infected RMs, while concurrently preserving the expression of its natural antisense lncRNA regulator MMP25- AS1. From a therapeutic standpoint, long-term low-dose THC administered as an adjunct to cART represents a potentially safe and effective intervention to dampen chronic immune activation and inflammation, a major driver of HIV-associated comorbidities in virally suppressed PLWH.

Article Snippet: Immunofluorescence studies for the detection of MMP25 (1:50) (Abcam; ab56309), TIMP2 (1:100) (Novus Biologicals; NBP2-01573), CD47 (1:200) (Mybiosource.com; MBS8106360), MPO (1:6,000) (Abcam; A0398), and CD11b (1:100) (Abcam; ab34216) were performed as described previously (16).

Techniques: Migration, Infection, Activation Assay, Disruption, Translocation Assay, Expressing, Blocking Assay, Preserving

Journal: PLoS ONE

Article Title: miR-4443 Participates in the Malignancy of Breast Cancer

doi: 10.1371/journal.pone.0160780

Figure Lengend Snippet: (A) , Interactions between TIMP2 and miR-4443 by TargetScan software. (B) , Quantification of TIMP2 mRNA mediated by miR-4443 in MDA-MB-231 cells. ΔCt values for mRNA are shown referenced to the expression of the endogenous control, β-actin. MDA-MB-231/Epi+inhibitors: MDA-MB-231/Epi transfected with miR-4443 Inhibitors; MDA-MB-231/S+mimics: MDA-MB-231/S transfected with miR-4443 mimics; NC: negative control of miR-4443 mimics or inhibitors. (C) , Relative protein expression of TIMP2, β-actin is the endogenous control. (D) , Quantification of TIMP2 mRNA in a)pre-neoadjuvant chemotherapy FFPE biopsies and b)surgically-resected FFPE specimens of 27 patients(p = 0.061). (E) , Survival curves of TIMP2(p<0.05).

Article Snippet: The membranes were blocked in 5% skim milk for 2 h and then probed with primary antibodies against TIMP2 (1:250; Biotechne, America) or β-actin (1:8000, Arigo, China) at 4°C overnight, followed by the secondary antibody (1:4000; Kangwei Ltd, China) for 1 h. Finally, after washing, enhanced chemiluminescence (ECL) plus kit (Millipore, America) was applied for visualization and β-actin was utilized as an internal control.

Techniques: Software, Expressing, Transfection, Negative Control

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Comprehensive stability analysis of 13 β-lactams and β-lactamase inhibitors in in vitro media, and novel supplement dosing strategy to mitigate thermal drug degradation

doi: 10.1128/aac.01399-23

Figure Lengend Snippet: Population PK estimated degradation half-lives (i.e., posthoc estimates) of β-lactams and β-lactamase inhibitors at different pH values in CA-MHB at 36°C a , b , c

Article Snippet: Ceftazidime pentahydrate, cefepime dihydrochloride monohydrate, cefsulodin sodium salt hydrate, piperacillin sodium salt, ticarcillin disodium salt, aztreonam, and tazobactam sodium salt were purchased from Chem-Impex International (Wood Dale, IL) and sulbactam from TCI America (Portland, OR).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Comprehensive stability analysis of 13 β-lactams and β-lactamase inhibitors in in vitro media, and novel supplement dosing strategy to mitigate thermal drug degradation

doi: 10.1128/aac.01399-23

Figure Lengend Snippet: Optimal dosing schemes with one supplement dose at 9 h

Article Snippet: Ceftazidime pentahydrate, cefepime dihydrochloride monohydrate, cefsulodin sodium salt hydrate, piperacillin sodium salt, ticarcillin disodium salt, aztreonam, and tazobactam sodium salt were purchased from Chem-Impex International (Wood Dale, IL) and sulbactam from TCI America (Portland, OR).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Comprehensive stability analysis of 13 β-lactams and β-lactamase inhibitors in in vitro media, and novel supplement dosing strategy to mitigate thermal drug degradation

doi: 10.1128/aac.01399-23

Figure Lengend Snippet: Optimal dosing schemes with two supplement doses e , f

Article Snippet: Ceftazidime pentahydrate, cefepime dihydrochloride monohydrate, cefsulodin sodium salt hydrate, piperacillin sodium salt, ticarcillin disodium salt, aztreonam, and tazobactam sodium salt were purchased from Chem-Impex International (Wood Dale, IL) and sulbactam from TCI America (Portland, OR).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Comprehensive stability analysis of 13 β-lactams and β-lactamase inhibitors in in vitro media, and novel supplement dosing strategy to mitigate thermal drug degradation

doi: 10.1128/aac.01399-23

Figure Lengend Snippet: Thermal degradation half-lives of ten β-lactam antibiotics and three β-lactamase inhibitors in different matrices relevant for in vitro infection model experiments and susceptibility testing at different temperatures and pH c , d

Article Snippet: Ceftazidime pentahydrate, cefepime dihydrochloride monohydrate, cefsulodin sodium salt hydrate, piperacillin sodium salt, ticarcillin disodium salt, aztreonam, and tazobactam sodium salt were purchased from Chem-Impex International (Wood Dale, IL) and sulbactam from TCI America (Portland, OR).

Techniques: In Vitro, Infection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Comprehensive stability analysis of 13 β-lactams and β-lactamase inhibitors in in vitro media, and novel supplement dosing strategy to mitigate thermal drug degradation

doi: 10.1128/aac.01399-23

Figure Lengend Snippet: Fraction of drug remaining in agar incubated at 37 and 4°C for different durations a

Article Snippet: Ceftazidime pentahydrate, cefepime dihydrochloride monohydrate, cefsulodin sodium salt hydrate, piperacillin sodium salt, ticarcillin disodium salt, aztreonam, and tazobactam sodium salt were purchased from Chem-Impex International (Wood Dale, IL) and sulbactam from TCI America (Portland, OR).

Techniques: Incubation