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  • 99
    New England Biolabs xho i
    Structure of the <t>DNA</t> molecules used in this study. A . Structure of the parent c-DNA. B . l-DNA formed by restriction digest of c-DNA with the enzyme Xho I. The size of the linearized plasmid was 4272 bp, C . pcr-DNA (2209 bp) generated by site-specific primers that amplified the region of the c-DNA containing the HTLV promoter, the hOPG open reading frame, and the SV40 Poly-A site. D . Structure of the parent c-DNA used in transfection. E . l-DNA formed by digest of pEGFP-N2 with the enzyme Cla I, which cuts in the vector backbone. F . pcr-DNA (1713 bp) generated by primers that are complementary to the sequence upstream of the CMV promoter and downstream of the polyA site.
    Xho I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    New England Biolabs sapi xho i digested ptyb11
    Structure of the <t>DNA</t> molecules used in this study. A . Structure of the parent c-DNA. B . l-DNA formed by restriction digest of c-DNA with the enzyme Xho I. The size of the linearized plasmid was 4272 bp, C . pcr-DNA (2209 bp) generated by site-specific primers that amplified the region of the c-DNA containing the HTLV promoter, the hOPG open reading frame, and the SV40 Poly-A site. D . Structure of the parent c-DNA used in transfection. E . l-DNA formed by digest of pEGFP-N2 with the enzyme Cla I, which cuts in the vector backbone. F . pcr-DNA (1713 bp) generated by primers that are complementary to the sequence upstream of the CMV promoter and downstream of the polyA site.
    Sapi Xho I Digested Ptyb11, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    New England Biolabs xho i bmg bi
    Structure of the <t>DNA</t> molecules used in this study. A . Structure of the parent c-DNA. B . l-DNA formed by restriction digest of c-DNA with the enzyme Xho I. The size of the linearized plasmid was 4272 bp, C . pcr-DNA (2209 bp) generated by site-specific primers that amplified the region of the c-DNA containing the HTLV promoter, the hOPG open reading frame, and the SV40 Poly-A site. D . Structure of the parent c-DNA used in transfection. E . l-DNA formed by digest of pEGFP-N2 with the enzyme Cla I, which cuts in the vector backbone. F . pcr-DNA (1713 bp) generated by primers that are complementary to the sequence upstream of the CMV promoter and downstream of the polyA site.
    Xho I Bmg Bi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    New England Biolabs xho li
    Structure of the <t>DNA</t> molecules used in this study. A . Structure of the parent c-DNA. B . l-DNA formed by restriction digest of c-DNA with the enzyme Xho I. The size of the linearized plasmid was 4272 bp, C . pcr-DNA (2209 bp) generated by site-specific primers that amplified the region of the c-DNA containing the HTLV promoter, the hOPG open reading frame, and the SV40 Poly-A site. D . Structure of the parent c-DNA used in transfection. E . l-DNA formed by digest of pEGFP-N2 with the enzyme Cla I, which cuts in the vector backbone. F . pcr-DNA (1713 bp) generated by primers that are complementary to the sequence upstream of the CMV promoter and downstream of the polyA site.
    Xho Li, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    New England Biolabs xho i restriction endonucleases
    Structure of the <t>DNA</t> molecules used in this study. A . Structure of the parent c-DNA. B . l-DNA formed by restriction digest of c-DNA with the enzyme Xho I. The size of the linearized plasmid was 4272 bp, C . pcr-DNA (2209 bp) generated by site-specific primers that amplified the region of the c-DNA containing the HTLV promoter, the hOPG open reading frame, and the SV40 Poly-A site. D . Structure of the parent c-DNA used in transfection. E . l-DNA formed by digest of pEGFP-N2 with the enzyme Cla I, which cuts in the vector backbone. F . pcr-DNA (1713 bp) generated by primers that are complementary to the sequence upstream of the CMV promoter and downstream of the polyA site.
    Xho I Restriction Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    New England Biolabs xho i restriction enzyme
    Gel images of amplicons digested with restriction enzymes. (A) mtORF amplification and digestion with Sac I restriction enzyme differentiates mtORF type 1 ( P. meandrina and P. eydouxi ) from all other Pocillopora . (B) PocHistone amplification and digestion with <t>Xho</t> I restriction enzyme differentiates P. eydouxi from all other Pocillopora . (C) mtORF amplification and digestion with the Aci I restriction enzyme differentiates P. verrucosa from all other Pocillopora . (D) mtORF amplification and digestion with the AlwN I restriction enzyme differentiates P. ligulata from all other Pocillopora . (E) mtORF amplification and digestion with the Nla IV restriction enzyme differentiates P. acuta and P. damicornis from all other Pocillopora . (F) mtORF amplification and digestion with the Tsp 45I restriction enzyme differentiates P. damicornis from all other Pocillopora .
    Xho I Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    New England Biolabs nhe i xho i
    Gel images of amplicons digested with restriction enzymes. (A) mtORF amplification and digestion with Sac I restriction enzyme differentiates mtORF type 1 ( P. meandrina and P. eydouxi ) from all other Pocillopora . (B) PocHistone amplification and digestion with <t>Xho</t> I restriction enzyme differentiates P. eydouxi from all other Pocillopora . (C) mtORF amplification and digestion with the Aci I restriction enzyme differentiates P. verrucosa from all other Pocillopora . (D) mtORF amplification and digestion with the AlwN I restriction enzyme differentiates P. ligulata from all other Pocillopora . (E) mtORF amplification and digestion with the Nla IV restriction enzyme differentiates P. acuta and P. damicornis from all other Pocillopora . (F) mtORF amplification and digestion with the Tsp 45I restriction enzyme differentiates P. damicornis from all other Pocillopora .
    Nhe I Xho I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs new england biolabs restriction enzymes
    Gel images of amplicons digested with restriction enzymes. (A) mtORF amplification and digestion with Sac I restriction enzyme differentiates mtORF type 1 ( P. meandrina and P. eydouxi ) from all other Pocillopora . (B) PocHistone amplification and digestion with <t>Xho</t> I restriction enzyme differentiates P. eydouxi from all other Pocillopora . (C) mtORF amplification and digestion with the Aci I restriction enzyme differentiates P. verrucosa from all other Pocillopora . (D) mtORF amplification and digestion with the AlwN I restriction enzyme differentiates P. ligulata from all other Pocillopora . (E) mtORF amplification and digestion with the Nla IV restriction enzyme differentiates P. acuta and P. damicornis from all other Pocillopora . (F) mtORF amplification and digestion with the Tsp 45I restriction enzyme differentiates P. damicornis from all other Pocillopora .
    New England Biolabs Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    New England Biolabs xho i linker sequence
    Gel images of amplicons digested with restriction enzymes. (A) mtORF amplification and digestion with Sac I restriction enzyme differentiates mtORF type 1 ( P. meandrina and P. eydouxi ) from all other Pocillopora . (B) PocHistone amplification and digestion with <t>Xho</t> I restriction enzyme differentiates P. eydouxi from all other Pocillopora . (C) mtORF amplification and digestion with the Aci I restriction enzyme differentiates P. verrucosa from all other Pocillopora . (D) mtORF amplification and digestion with the AlwN I restriction enzyme differentiates P. ligulata from all other Pocillopora . (E) mtORF amplification and digestion with the Nla IV restriction enzyme differentiates P. acuta and P. damicornis from all other Pocillopora . (F) mtORF amplification and digestion with the Tsp 45I restriction enzyme differentiates P. damicornis from all other Pocillopora .
    Xho I Linker Sequence, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Structure of the DNA molecules used in this study. A . Structure of the parent c-DNA. B . l-DNA formed by restriction digest of c-DNA with the enzyme Xho I. The size of the linearized plasmid was 4272 bp, C . pcr-DNA (2209 bp) generated by site-specific primers that amplified the region of the c-DNA containing the HTLV promoter, the hOPG open reading frame, and the SV40 Poly-A site. D . Structure of the parent c-DNA used in transfection. E . l-DNA formed by digest of pEGFP-N2 with the enzyme Cla I, which cuts in the vector backbone. F . pcr-DNA (1713 bp) generated by primers that are complementary to the sequence upstream of the CMV promoter and downstream of the polyA site.

    Journal: BMC Biotechnology

    Article Title: Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers

    doi: 10.1186/1472-6750-8-23

    Figure Lengend Snippet: Structure of the DNA molecules used in this study. A . Structure of the parent c-DNA. B . l-DNA formed by restriction digest of c-DNA with the enzyme Xho I. The size of the linearized plasmid was 4272 bp, C . pcr-DNA (2209 bp) generated by site-specific primers that amplified the region of the c-DNA containing the HTLV promoter, the hOPG open reading frame, and the SV40 Poly-A site. D . Structure of the parent c-DNA used in transfection. E . l-DNA formed by digest of pEGFP-N2 with the enzyme Cla I, which cuts in the vector backbone. F . pcr-DNA (1713 bp) generated by primers that are complementary to the sequence upstream of the CMV promoter and downstream of the polyA site.

    Article Snippet: Linear DNA (l-DNA) Purified c-DNA was linearized using the restriction enzyme, Xho I (New England Biolabs; Pickering, ON) for pORF9-hTNFRS11b or Cla I (Invitrogen; Burlington, ON) for pEGFP-N2, Restriction digestion were set up with 5 μg of DNA per 50 μL of reaction volume containing 3 units of enzyme and incubated at 37°C for 16 hours.

    Techniques: Plasmid Preparation, Polymerase Chain Reaction, Generated, Amplification, Transfection, Sequencing

    RDA-derived RE fragments of MDV (strain CVI). The RE fragments were prepared by subtraction of Bam HI-, Bgl II-, Eco RI-, Hin dIII-, or Xho I-digested CEF DNA from those of MDV-infected CEF (see Materials and Methods). (A) Agarose gel electrophoresis of RDA-derived fragments amplified from Bgl II-, Eco RI-, Hin dIII-, or Xho I-digested MDV DNAs. CEF indicates the negative control of RDA. (B) Southern blot analysis of the gel shown in panel A. A sequence corresponding to that of the MDV genome was detected with 32 P-labeled MDV genomic clones. (C) Southern blot analysis of RE fragments of MDV. The probe was the same as above. Numbers between panels indicate sizes of markers in kilobase pairs.

    Journal: Journal of Clinical Microbiology

    Article Title: Application of Representational Difference Analysis to Genomic Fragments of Marek's Disease Virus

    doi:

    Figure Lengend Snippet: RDA-derived RE fragments of MDV (strain CVI). The RE fragments were prepared by subtraction of Bam HI-, Bgl II-, Eco RI-, Hin dIII-, or Xho I-digested CEF DNA from those of MDV-infected CEF (see Materials and Methods). (A) Agarose gel electrophoresis of RDA-derived fragments amplified from Bgl II-, Eco RI-, Hin dIII-, or Xho I-digested MDV DNAs. CEF indicates the negative control of RDA. (B) Southern blot analysis of the gel shown in panel A. A sequence corresponding to that of the MDV genome was detected with 32 P-labeled MDV genomic clones. (C) Southern blot analysis of RE fragments of MDV. The probe was the same as above. Numbers between panels indicate sizes of markers in kilobase pairs.

    Article Snippet: One microgram of cellular DNAs was digested with units of RE— Bam HI, Bgl II, Eco RI, Hin dIII, Sal I, or Xho I—under the conditions recommended by the manufacturer of the RE (New England BioLabs, Inc., Beverly, Mass.).

    Techniques: Derivative Assay, Infection, Agarose Gel Electrophoresis, Amplification, Negative Control, Southern Blot, Sequencing, Labeling

    Fig. 1. Overexpression of SeqA protein extends the period of hemimethylation at oriC . ( A ) Schematic representation of the temperature shifts at the indicated times during the experiment. ( B ) Exponentially growing cultures (30°C) of MG1655 dnaC2 cells harbouring pFH2102 (control) or pMAK7 (excess SeqA) were synchronized with respect to initiation for 1 h at 38°C before shifting the temperature to 30°C. IPTG (0.5 mM) was added to the cultures three mass doublings prior to synchronized initiation. Chromosomal DNA was extracted at the indicated times after returning the cultures to 30°C. The DNA was digested with Xho I and Hph I, electrophoresed and hybridized to an oriC probe. The positions of cut and uncut fragments are indicated by arrows. The rightmost lane contains DNA isolated from a dam – strain which reveals the position of the cut fragment. ( C ) Quantification of the amount of hemimethylated oriC in (B). Squares represent dnaC 2/pFH2102 and triangles dnaC 2/pMAK7. The percentage of hemimethylated oriC is twice the percentage of the cut fragment in (B) since 50% of the hemimethylated fragments are digested. Time indicates minutes from shift to 30°C after 1 h synchronization at 38°C. ( D ) Western analyses of samples taken from the cultures at 0 and 20 min after returning the cultures to 30°C. A 1 µg aliquot of total protein of each sample was loaded.

    Journal: The EMBO Journal

    Article Title: Excess SeqA prolongs sequestration of oriC and delays nucleoid segregation and cell division

    doi: 10.1093/emboj/cdg020

    Figure Lengend Snippet: Fig. 1. Overexpression of SeqA protein extends the period of hemimethylation at oriC . ( A ) Schematic representation of the temperature shifts at the indicated times during the experiment. ( B ) Exponentially growing cultures (30°C) of MG1655 dnaC2 cells harbouring pFH2102 (control) or pMAK7 (excess SeqA) were synchronized with respect to initiation for 1 h at 38°C before shifting the temperature to 30°C. IPTG (0.5 mM) was added to the cultures three mass doublings prior to synchronized initiation. Chromosomal DNA was extracted at the indicated times after returning the cultures to 30°C. The DNA was digested with Xho I and Hph I, electrophoresed and hybridized to an oriC probe. The positions of cut and uncut fragments are indicated by arrows. The rightmost lane contains DNA isolated from a dam – strain which reveals the position of the cut fragment. ( C ) Quantification of the amount of hemimethylated oriC in (B). Squares represent dnaC 2/pFH2102 and triangles dnaC 2/pMAK7. The percentage of hemimethylated oriC is twice the percentage of the cut fragment in (B) since 50% of the hemimethylated fragments are digested. Time indicates minutes from shift to 30°C after 1 h synchronization at 38°C. ( D ) Western analyses of samples taken from the cultures at 0 and 20 min after returning the cultures to 30°C. A 1 µg aliquot of total protein of each sample was loaded.

    Article Snippet: Restriction enzyme digestions to identify hemimethylated DNA were performed by incubating 1 µg of chromosomal DNA and 3 U of Hph I and Xho I restriction enzymes (New England Biolabs) for 1.5 h at 37°C ( ).

    Techniques: Over Expression, Isolation, Western Blot

    Gel images of amplicons digested with restriction enzymes. (A) mtORF amplification and digestion with Sac I restriction enzyme differentiates mtORF type 1 ( P. meandrina and P. eydouxi ) from all other Pocillopora . (B) PocHistone amplification and digestion with Xho I restriction enzyme differentiates P. eydouxi from all other Pocillopora . (C) mtORF amplification and digestion with the Aci I restriction enzyme differentiates P. verrucosa from all other Pocillopora . (D) mtORF amplification and digestion with the AlwN I restriction enzyme differentiates P. ligulata from all other Pocillopora . (E) mtORF amplification and digestion with the Nla IV restriction enzyme differentiates P. acuta and P. damicornis from all other Pocillopora . (F) mtORF amplification and digestion with the Tsp 45I restriction enzyme differentiates P. damicornis from all other Pocillopora .

    Journal: PeerJ

    Article Title: A simple molecular technique for distinguishing species reveals frequent misidentification of Hawaiian corals in the genus Pocillopora

    doi: 10.7717/peerj.4355

    Figure Lengend Snippet: Gel images of amplicons digested with restriction enzymes. (A) mtORF amplification and digestion with Sac I restriction enzyme differentiates mtORF type 1 ( P. meandrina and P. eydouxi ) from all other Pocillopora . (B) PocHistone amplification and digestion with Xho I restriction enzyme differentiates P. eydouxi from all other Pocillopora . (C) mtORF amplification and digestion with the Aci I restriction enzyme differentiates P. verrucosa from all other Pocillopora . (D) mtORF amplification and digestion with the AlwN I restriction enzyme differentiates P. ligulata from all other Pocillopora . (E) mtORF amplification and digestion with the Nla IV restriction enzyme differentiates P. acuta and P. damicornis from all other Pocillopora . (F) mtORF amplification and digestion with the Tsp 45I restriction enzyme differentiates P. damicornis from all other Pocillopora .

    Article Snippet: PCR products were digested with 0.5 μL of Xho I restriction enzyme (New England BioLabs, Ipswich, MA, USA) and 0.5 μL of 1X CutSmart® buffer for 1 hr at 37 °C, followed by heat inactivation at 65 °C for 20 min ( ).

    Techniques: Amplification