φx174 rfii dna Search Results


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  • 95
    New England Biolabs psii
    Psii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher polyacrylamide tbe gels
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
    Polyacrylamide Tbe Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega mung bean nuclease
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
    Mung Bean Nuclease, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs cutsmart buffer
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
    Cutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2628 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega dnase i
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
    Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 14104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    New England Biolabs sulfolobus dna polymerase iv
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
    Sulfolobus Dna Polymerase Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs diluent e
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
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    99
    New England Biolabs low molecular weight lmw dna ladders
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
    Low Molecular Weight Lmw Dna Ladders, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs nebuffer 3 1
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
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    New England Biolabs nebuffer 4
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
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    Qiagen qiaprep spin miniprep kit
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
    Qiaprep Spin Miniprep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 42567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiaquick gel extraction kit
    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on <t>Novex</t> 20% polyacrylamide <t>TBE</t> gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.
    Qiaquick Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 113321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on Novex 20% polyacrylamide TBE gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.

    Journal: PLoS ONE

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro

    doi: 10.1371/journal.pone.0203073

    Figure Lengend Snippet: ET SSB reduces off-target activity with Tt Ago. Sets of two guides targeting different locations on ΦX174 Virion DNA over increasing concentration of ET SSB. Reactions were carried out at 74 °C for 5 hr and products were analyzed on Novex 20% polyacrylamide TBE gels stained with 3X GelRed in water. Off target cutting is reduced with higher concentrations of ET SSB. ( A ) 528 nt product using ΦX174 guides 3 and 5; ( B ) 728 nt product using ΦX174 guides 3 and 6; ( C ) 359 nt product using ΦX174 guides 1 and 4; ( D ) 779 nt product using ΦX174 guides 2 and 6.

    Article Snippet: Novex™ 20% polyacrylamide TBE Gels were obtained from Thermo Fisher Scientific, Inc. (Waltham, Massachusetts, USA).

    Techniques: Activity Assay, Concentration Assay, Staining