γ-hbcd concentrations Search Results


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  • 99
    Millipore 2 hbcd
    Representative photomicrographs illustrating the number of Fos-positive neurons in the rat brain after single exposure to antipsychotic agents. <t>HBC,</t> <t>2-hydroxypropyl-β-cyclodextrin;</t> ARI, aripiprazole-treated; mPFC, medial prefrontal cortex; NAC, nucleus accumbens; DG, dentate gyrus; Ce, central amygdala; BL, basolateral amygdala; Tc, temporal cortex. N=8 rats/group. Scale bar: 100 um.
    2 Hbcd, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad hepatitis b surface antigen
    Representative photomicrographs illustrating the number of Fos-positive neurons in the rat brain after single exposure to antipsychotic agents. <t>HBC,</t> <t>2-hydroxypropyl-β-cyclodextrin;</t> ARI, aripiprazole-treated; mPFC, medial prefrontal cortex; NAC, nucleus accumbens; DG, dentate gyrus; Ce, central amygdala; BL, basolateral amygdala; Tc, temporal cortex. N=8 rats/group. Scale bar: 100 um.
    Hepatitis B Surface Antigen, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    DiaSorin igm anti hbc
    Representative photomicrographs illustrating the number of Fos-positive neurons in the rat brain after single exposure to antipsychotic agents. <t>HBC,</t> <t>2-hydroxypropyl-β-cyclodextrin;</t> ARI, aripiprazole-treated; mPFC, medial prefrontal cortex; NAC, nucleus accumbens; DG, dentate gyrus; Ce, central amygdala; BL, basolateral amygdala; Tc, temporal cortex. N=8 rats/group. Scale bar: 100 um.
    Igm Anti Hbc, supplied by DiaSorin, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Abbott Laboratories hepatitis b core antibodies anti hbc
    Representative photomicrographs illustrating the number of Fos-positive neurons in the rat brain after single exposure to antipsychotic agents. <t>HBC,</t> <t>2-hydroxypropyl-β-cyclodextrin;</t> ARI, aripiprazole-treated; mPFC, medial prefrontal cortex; NAC, nucleus accumbens; DG, dentate gyrus; Ce, central amygdala; BL, basolateral amygdala; Tc, temporal cortex. N=8 rats/group. Scale bar: 100 um.
    Hepatitis B Core Antibodies Anti Hbc, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies triple quadrupole mass spectrometer
    Representative photomicrographs illustrating the number of Fos-positive neurons in the rat brain after single exposure to antipsychotic agents. <t>HBC,</t> <t>2-hydroxypropyl-β-cyclodextrin;</t> ARI, aripiprazole-treated; mPFC, medial prefrontal cortex; NAC, nucleus accumbens; DG, dentate gyrus; Ce, central amygdala; BL, basolateral amygdala; Tc, temporal cortex. N=8 rats/group. Scale bar: 100 um.
    Triple Quadrupole Mass Spectrometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Millipore hydroxypropyl β cyclodextrin hpβcd
    Antioxidant activity of DOPE in the β-carotene emulsion model system. ( a ): β-carotene absorbance inhibition ratio during 120 min incubation; ( b ): antioxidant activity expressed as IC 50. nDOPE-native dry olive pomace extract; hpDOPE-extract prepared with <t>hydroxypropyl-β-cyclodextrin;</t> ramDOPE-extract prepared with randomly methylated β-cyclodextrin; BHA: butylhydroxyanisol; IC 50 : half maximal inhibitory concentration. Columns (in Figure 3 b) marked with different letters belong to different statistical groups ( p
    Hydroxypropyl β Cyclodextrin Hpβcd, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative photomicrographs illustrating the number of Fos-positive neurons in the rat brain after single exposure to antipsychotic agents. HBC, 2-hydroxypropyl-β-cyclodextrin; ARI, aripiprazole-treated; mPFC, medial prefrontal cortex; NAC, nucleus accumbens; DG, dentate gyrus; Ce, central amygdala; BL, basolateral amygdala; Tc, temporal cortex. N=8 rats/group. Scale bar: 100 um.

    Journal: Clinical Psychopharmacology and Neuroscience

    Article Title: Effects of Aripiprazole and Haloperidol on Fos-like Immunoreactivity in the Prefrontal Cortex and Amygdala

    doi: 10.9758/cpn.2011.9.1.36

    Figure Lengend Snippet: Representative photomicrographs illustrating the number of Fos-positive neurons in the rat brain after single exposure to antipsychotic agents. HBC, 2-hydroxypropyl-β-cyclodextrin; ARI, aripiprazole-treated; mPFC, medial prefrontal cortex; NAC, nucleus accumbens; DG, dentate gyrus; Ce, central amygdala; BL, basolateral amygdala; Tc, temporal cortex. N=8 rats/group. Scale bar: 100 um.

    Article Snippet: Haloperidol and aripiprazole were dissolved in 0.1 M tartaric acid and 45% 2-hydroxypropyl-β-cyclodextrin (HBC) (Sigma, USA), respectively.

    Techniques:

    Acute cholesterol depletion of ob/ob and wild-type hepatocytes. Hepatocytes were pretreated with 20 mM 2-hydroxypropyl-β-cyclodextrin for 30 minutes, washed, then incubated with 5 μg/mL of 125 I and 3 H cholesteryl ether–labeled apoE-free HDL at 37°C for 1 hour. ( a ) HDL protein association ( 125 I). ( b ) Apparent selective uptake was measured by subtracting the amount of HDL cholesteryl ether ( 3 H) from the amount of HDL protein ( 125 I) that remained cell associated. This experiment was performed a total of 2 times, in triplicate, with similar results. Results were reported as mean nanogram HDL or HDL cholesteryl ether per milligram cell protein per hour ± SD, after subtraction of background counts measured in the presence of 100-fold excess unlabeled HDL.

    Journal: Journal of Clinical Investigation

    Article Title: Defective HDL particle uptake in ob/ob hepatocytes causes decreased recycling, degradation, and selective lipid uptake

    doi:

    Figure Lengend Snippet: Acute cholesterol depletion of ob/ob and wild-type hepatocytes. Hepatocytes were pretreated with 20 mM 2-hydroxypropyl-β-cyclodextrin for 30 minutes, washed, then incubated with 5 μg/mL of 125 I and 3 H cholesteryl ether–labeled apoE-free HDL at 37°C for 1 hour. ( a ) HDL protein association ( 125 I). ( b ) Apparent selective uptake was measured by subtracting the amount of HDL cholesteryl ether ( 3 H) from the amount of HDL protein ( 125 I) that remained cell associated. This experiment was performed a total of 2 times, in triplicate, with similar results. Results were reported as mean nanogram HDL or HDL cholesteryl ether per milligram cell protein per hour ± SD, after subtraction of background counts measured in the presence of 100-fold excess unlabeled HDL.

    Article Snippet: Isolated hepatocytes were incubated with 20 mM 2-hydroxypropyl-β-cyclodextrin (Sigma) for 30 minutes, washed to remove the cyclodextrin, and subsequently incubated with double-labeled HDL (125 I and 3 H cholesteryl ether) for 1 hour at 37°C.

    Techniques: Incubation, Labeling

    Liver histology and biochemistry in NPC cats administered SC or IC HPβCD

    Journal: Science translational medicine

    Article Title: Intracisternal Cyclodextrin Prevents Cerebellar Dysfunction and Purkinje Cell Death in Feline Niemann-Pick type C1 disease

    doi: 10.1126/scitranslmed.3010101

    Figure Lengend Snippet: Liver histology and biochemistry in NPC cats administered SC or IC HPβCD

    Article Snippet: The powdered form of HPβCD (HPβCD-H107; Sigma Aldrich, St Louis MO) was used for subcutaneous injections as high doses were necessary and this was the least expensive formulation available.

    Techniques:

    Pulmonary histology from 6-month-old untreated NPC cats and NPC cats administered 8000 mg/kg SC HPβCD

    Journal: Science translational medicine

    Article Title: Intracisternal Cyclodextrin Prevents Cerebellar Dysfunction and Purkinje Cell Death in Feline Niemann-Pick type C1 disease

    doi: 10.1126/scitranslmed.3010101

    Figure Lengend Snippet: Pulmonary histology from 6-month-old untreated NPC cats and NPC cats administered 8000 mg/kg SC HPβCD

    Article Snippet: The powdered form of HPβCD (HPβCD-H107; Sigma Aldrich, St Louis MO) was used for subcutaneous injections as high doses were necessary and this was the least expensive formulation available.

    Techniques:

    Calbindin and GM2 immunohistochemical staining and filipin staining of cerebellum from normal control, untreated NPC, and HPβCD-treated NPC cats

    Journal: Science translational medicine

    Article Title: Intracisternal Cyclodextrin Prevents Cerebellar Dysfunction and Purkinje Cell Death in Feline Niemann-Pick type C1 disease

    doi: 10.1126/scitranslmed.3010101

    Figure Lengend Snippet: Calbindin and GM2 immunohistochemical staining and filipin staining of cerebellum from normal control, untreated NPC, and HPβCD-treated NPC cats

    Article Snippet: The powdered form of HPβCD (HPβCD-H107; Sigma Aldrich, St Louis MO) was used for subcutaneous injections as high doses were necessary and this was the least expensive formulation available.

    Techniques: Immunohistochemistry, Staining

    Age of onset and severity of ataxia in untreated NPC cats and NPC cats administered IC HPβCD

    Journal: Science translational medicine

    Article Title: Intracisternal Cyclodextrin Prevents Cerebellar Dysfunction and Purkinje Cell Death in Feline Niemann-Pick type C1 disease

    doi: 10.1126/scitranslmed.3010101

    Figure Lengend Snippet: Age of onset and severity of ataxia in untreated NPC cats and NPC cats administered IC HPβCD

    Article Snippet: The powdered form of HPβCD (HPβCD-H107; Sigma Aldrich, St Louis MO) was used for subcutaneous injections as high doses were necessary and this was the least expensive formulation available.

    Techniques:

    Cerebral gray matter lipids in normal control, untreated NPC, and NPC cats administered IC HPβCD

    Journal: Science translational medicine

    Article Title: Intracisternal Cyclodextrin Prevents Cerebellar Dysfunction and Purkinje Cell Death in Feline Niemann-Pick type C1 disease

    doi: 10.1126/scitranslmed.3010101

    Figure Lengend Snippet: Cerebral gray matter lipids in normal control, untreated NPC, and NPC cats administered IC HPβCD

    Article Snippet: The powdered form of HPβCD (HPβCD-H107; Sigma Aldrich, St Louis MO) was used for subcutaneous injections as high doses were necessary and this was the least expensive formulation available.

    Techniques:

    Intracisternal HPβCD resulted in neurologically normal cats at 24 weeks of age

    Journal: Science translational medicine

    Article Title: Intracisternal Cyclodextrin Prevents Cerebellar Dysfunction and Purkinje Cell Death in Feline Niemann-Pick type C1 disease

    doi: 10.1126/scitranslmed.3010101

    Figure Lengend Snippet: Intracisternal HPβCD resulted in neurologically normal cats at 24 weeks of age

    Article Snippet: The powdered form of HPβCD (HPβCD-H107; Sigma Aldrich, St Louis MO) was used for subcutaneous injections as high doses were necessary and this was the least expensive formulation available.

    Techniques:

    Carbonitrile-chalcone reduces inflammation in a mouse model of allergic eosinophilic airway inflammation. A , in vitro inhibition of CXCL12 binding to CXCR4 receptor by chalcone 4 ( Chalc 4 ) and CN-chalcone 4 ( CN-Chalc 4 ). Inhibition of CXCL12 binding to CXCR4 as a function of increasing concentration of chalcone 4 ( gray squares ) and CN-chalcone 4 ( black circles ) is monitored using FRET intensity variation. The fluorescence of cells expressing EGFP-labeled CXCR4 is followed at 510 nm as a function of time. Upon the addition of Texas Red-labeled CXCL12 ( CXCL12-TR , 100 n m ), fluorescence intensity at 510 nm declines as a result of interaction between CXCL12-TR and EGFP-CXCR4, which causes FRET. The ordinate axis reports the intensity of FRET as a percentage of the control value (100 n m CXCL12-TR alone). K i values were derived from the IC 50 values determined from competition curves using the Cheng and Prusoff relationship ( 65 ). K i values are 53 ± 31 n m (chalcone 4) and 45 ± 57 n m (CN-chalcone 4). Each data point represents the mean ± S.D. ( error bars ) of three experiments. B , in vivo dose-response effect of a topical treatment with chalcone 4 and CN-chalcone 4 in an 8-day mouse model of hypereosinophilia. BALB/c mice were sensitized and challenged with OVA or saline. Chalcone 4 ( gray line ) or CN-chalcone 4 ( black line ) solubilized in 10% HP-βCD were administered intranasally 2 h before each challenge. The percentage of inhibition of eosinophil is shown. Data points ( squares ) are means of n = 6 determinations. C and D , effect of topical (intranasal; i.n. ) treatment with chalcone 4, CN-chalcone 4 ( C ), and chalcone 1 ( Chalc 1 ) ( D ) in the 8-day mouse model of hypereosinophilia. BALB/c mice were sensitized and challenged with OVA or saline. Drugs (300 nmol/kg) were administered intranasally 2 h before each challenge in HP-βCD 10% (vehicle). Absolute numbers of macrophages, eosinophils, neutrophils, and lymphocytes in BALF are shown. Bars represent means, and error bars show S.E. values ( n = 6/group). *, p ≤ 0.05 in comparison with the saline-treated OVA group.

    Journal: The Journal of Biological Chemistry

    Article Title: An Antedrug of the CXCL12 Neutraligand Blocks Experimental Allergic Asthma without Systemic Effect in Mice *

    doi: 10.1074/jbc.M112.449348

    Figure Lengend Snippet: Carbonitrile-chalcone reduces inflammation in a mouse model of allergic eosinophilic airway inflammation. A , in vitro inhibition of CXCL12 binding to CXCR4 receptor by chalcone 4 ( Chalc 4 ) and CN-chalcone 4 ( CN-Chalc 4 ). Inhibition of CXCL12 binding to CXCR4 as a function of increasing concentration of chalcone 4 ( gray squares ) and CN-chalcone 4 ( black circles ) is monitored using FRET intensity variation. The fluorescence of cells expressing EGFP-labeled CXCR4 is followed at 510 nm as a function of time. Upon the addition of Texas Red-labeled CXCL12 ( CXCL12-TR , 100 n m ), fluorescence intensity at 510 nm declines as a result of interaction between CXCL12-TR and EGFP-CXCR4, which causes FRET. The ordinate axis reports the intensity of FRET as a percentage of the control value (100 n m CXCL12-TR alone). K i values were derived from the IC 50 values determined from competition curves using the Cheng and Prusoff relationship ( 65 ). K i values are 53 ± 31 n m (chalcone 4) and 45 ± 57 n m (CN-chalcone 4). Each data point represents the mean ± S.D. ( error bars ) of three experiments. B , in vivo dose-response effect of a topical treatment with chalcone 4 and CN-chalcone 4 in an 8-day mouse model of hypereosinophilia. BALB/c mice were sensitized and challenged with OVA or saline. Chalcone 4 ( gray line ) or CN-chalcone 4 ( black line ) solubilized in 10% HP-βCD were administered intranasally 2 h before each challenge. The percentage of inhibition of eosinophil is shown. Data points ( squares ) are means of n = 6 determinations. C and D , effect of topical (intranasal; i.n. ) treatment with chalcone 4, CN-chalcone 4 ( C ), and chalcone 1 ( Chalc 1 ) ( D ) in the 8-day mouse model of hypereosinophilia. BALB/c mice were sensitized and challenged with OVA or saline. Drugs (300 nmol/kg) were administered intranasally 2 h before each challenge in HP-βCD 10% (vehicle). Absolute numbers of macrophages, eosinophils, neutrophils, and lymphocytes in BALF are shown. Bars represent means, and error bars show S.E. values ( n = 6/group). *, p ≤ 0.05 in comparison with the saline-treated OVA group.

    Article Snippet: In a first set of experiments, mice received either chalcone 4 or carbonitrile-chalcone 4 solubilized in PBS with 10% HP-βCD (C0926, Sigma) by intranasal injection 2 h before each nasal OVA or saline challenge.

    Techniques: In Vitro, Inhibition, Binding Assay, Concentration Assay, Fluorescence, Expressing, Labeling, Derivative Assay, In Vivo, Mouse Assay

    CN-chalcone 4 degradation products are inactive. A , CN-chalcone 4 ( CN-Chalc 4 ) degradation products (up to 10 μ m ) do not inhibit CXCL12-TR binding to EGFP-CXCR4 detected by FRET as in Fig. 1 A . Each data point represents the mean ± S.D. of three independent experiments performed in triplicates. B , CN-chalcone 4, but not its degradation products, dose-dependently inhibits CXCL12 action on forskolin-evoked cAMP responses in HEK EGFP-CXCR4 cells. The first two bars report the maximal production of cAMP (%) triggered by 1 μ m forskolin and its inhibition by 3 n m CXCL12. The following bars show that chalcone 4 ( Chalc 4 ) and CN-chalcone 4 (1, 3, and 10 μ m ), but not vanillin or pCBA (10 μ m ), inhibit the CXCL12 effect on cAMP production. Each bar represents the mean ± S.D. ( error bars ) of three independent experiments performed in triplicates. C , topical treatment with chalcone 4, CN-chalcone 4, vanillin, and pCBA in the 8-day mouse model of hypereosinophilia. BALB/c mice were immunized and challenged with OVA or saline. Treatments (300 nmol/kg) or HP-βCD 10% (vehicle), were administered intranasally 2 h before each challenge. Absolute numbers of macrophages, eosinophils, neutrophils, and lymphocytes in BAL are shown. Bars show means, and error bars show S.E. values ( n = 6/group). *, p ≤ 0.05 in comparison with the saline-treated OVA group.

    Journal: The Journal of Biological Chemistry

    Article Title: An Antedrug of the CXCL12 Neutraligand Blocks Experimental Allergic Asthma without Systemic Effect in Mice *

    doi: 10.1074/jbc.M112.449348

    Figure Lengend Snippet: CN-chalcone 4 degradation products are inactive. A , CN-chalcone 4 ( CN-Chalc 4 ) degradation products (up to 10 μ m ) do not inhibit CXCL12-TR binding to EGFP-CXCR4 detected by FRET as in Fig. 1 A . Each data point represents the mean ± S.D. of three independent experiments performed in triplicates. B , CN-chalcone 4, but not its degradation products, dose-dependently inhibits CXCL12 action on forskolin-evoked cAMP responses in HEK EGFP-CXCR4 cells. The first two bars report the maximal production of cAMP (%) triggered by 1 μ m forskolin and its inhibition by 3 n m CXCL12. The following bars show that chalcone 4 ( Chalc 4 ) and CN-chalcone 4 (1, 3, and 10 μ m ), but not vanillin or pCBA (10 μ m ), inhibit the CXCL12 effect on cAMP production. Each bar represents the mean ± S.D. ( error bars ) of three independent experiments performed in triplicates. C , topical treatment with chalcone 4, CN-chalcone 4, vanillin, and pCBA in the 8-day mouse model of hypereosinophilia. BALB/c mice were immunized and challenged with OVA or saline. Treatments (300 nmol/kg) or HP-βCD 10% (vehicle), were administered intranasally 2 h before each challenge. Absolute numbers of macrophages, eosinophils, neutrophils, and lymphocytes in BAL are shown. Bars show means, and error bars show S.E. values ( n = 6/group). *, p ≤ 0.05 in comparison with the saline-treated OVA group.

    Article Snippet: In a first set of experiments, mice received either chalcone 4 or carbonitrile-chalcone 4 solubilized in PBS with 10% HP-βCD (C0926, Sigma) by intranasal injection 2 h before each nasal OVA or saline challenge.

    Techniques: Binding Assay, Inhibition, Mouse Assay

    Cytotoxicity of chalcone derivatives on HepG2 cells using a resazurin reduction assay. Cell viability is expressed as the percentage of untreated control cells ( A ) and as 10% HP-βCD-treated cells ( B ). Cells were exposed to 10 μ m compounds for 24 h. Each exposure was preceded by equilibrium setting for 16 h in growth medium at 37 °C, 5% CO 2 . The dye was added to the cells together with the test substances at a final concentration of 10%. Simvastatin (100 μ m ) was used as a positive control. All samples contained 0.1% DMSO. Data are expressed as means ± S.D. ( error bars ) ( n = 3). Chalc 4 , chalcone 4; CN-Chalc 4 , CN-chalcone 4.

    Journal: The Journal of Biological Chemistry

    Article Title: An Antedrug of the CXCL12 Neutraligand Blocks Experimental Allergic Asthma without Systemic Effect in Mice *

    doi: 10.1074/jbc.M112.449348

    Figure Lengend Snippet: Cytotoxicity of chalcone derivatives on HepG2 cells using a resazurin reduction assay. Cell viability is expressed as the percentage of untreated control cells ( A ) and as 10% HP-βCD-treated cells ( B ). Cells were exposed to 10 μ m compounds for 24 h. Each exposure was preceded by equilibrium setting for 16 h in growth medium at 37 °C, 5% CO 2 . The dye was added to the cells together with the test substances at a final concentration of 10%. Simvastatin (100 μ m ) was used as a positive control. All samples contained 0.1% DMSO. Data are expressed as means ± S.D. ( error bars ) ( n = 3). Chalc 4 , chalcone 4; CN-Chalc 4 , CN-chalcone 4.

    Article Snippet: In a first set of experiments, mice received either chalcone 4 or carbonitrile-chalcone 4 solubilized in PBS with 10% HP-βCD (C0926, Sigma) by intranasal injection 2 h before each nasal OVA or saline challenge.

    Techniques: Concentration Assay, Positive Control

    CN-chalcone 4 is an antedrug. Systemic effect of chalcone 4 and CN-chalcone 4 in two vehicles. Systemic (intraperitoneal ( i.p. )) treatment with chalcone 4 ( Chalc 4 ) and CN-chalcone 4 ( CN-Chalc 4 ) in the 8-day mouse model of hypereosinophilia is shown. BALB/c mice were sensitized and challenged with OVA or saline. Drugs (two administrations of 20 μmol/kg/day for 3 days in 10% HP-βCD (vehicle) ( A ) or 350 μmol/kg once a day for 3 days in 1% carboxymethylcellulose ( CMC ) as a vehicle ( B )) were administered intraperitoneally 2 h before each OVA challenge. Absolute numbers of macrophages, eosinophils ( top ), neutrophils, and lymphocytes ( middle ) in BALF are shown. Bars show means, and error bars show S.E. values ( n = 6/group). *, p ≤ 0.05 in comparison with the saline-treated OVA group. Dose intensity relationship of eosinophil recruitment in the intraperitoneal route is shown for chalcone 4 and CN-chalcone 4 up to the maximal dose ( bottom ).

    Journal: The Journal of Biological Chemistry

    Article Title: An Antedrug of the CXCL12 Neutraligand Blocks Experimental Allergic Asthma without Systemic Effect in Mice *

    doi: 10.1074/jbc.M112.449348

    Figure Lengend Snippet: CN-chalcone 4 is an antedrug. Systemic effect of chalcone 4 and CN-chalcone 4 in two vehicles. Systemic (intraperitoneal ( i.p. )) treatment with chalcone 4 ( Chalc 4 ) and CN-chalcone 4 ( CN-Chalc 4 ) in the 8-day mouse model of hypereosinophilia is shown. BALB/c mice were sensitized and challenged with OVA or saline. Drugs (two administrations of 20 μmol/kg/day for 3 days in 10% HP-βCD (vehicle) ( A ) or 350 μmol/kg once a day for 3 days in 1% carboxymethylcellulose ( CMC ) as a vehicle ( B )) were administered intraperitoneally 2 h before each OVA challenge. Absolute numbers of macrophages, eosinophils ( top ), neutrophils, and lymphocytes ( middle ) in BALF are shown. Bars show means, and error bars show S.E. values ( n = 6/group). *, p ≤ 0.05 in comparison with the saline-treated OVA group. Dose intensity relationship of eosinophil recruitment in the intraperitoneal route is shown for chalcone 4 and CN-chalcone 4 up to the maximal dose ( bottom ).

    Article Snippet: In a first set of experiments, mice received either chalcone 4 or carbonitrile-chalcone 4 solubilized in PBS with 10% HP-βCD (C0926, Sigma) by intranasal injection 2 h before each nasal OVA or saline challenge.

    Techniques: Mouse Assay

    Effects of HP-β-CD compared to SBE-β-CD in cultured cells from Npc1 −/− mice. Thioglycollate-elicited peritoneal (from 49-day-old mice) and hippocampal neurons (from neonatal mouse pups) were harvested from Npc1 +/+ and Npc1 −/− mice. These cells were treated in FBS-containing culture media with vehicle (saline), 0.3 mM HP-β-CD, or 0.3 mM SBE-β-CD. After 16 h, the cells were harvested for RNA, and the relative mRNA levels of SREBP2 target genes Hmg CoA Syn (A) and Ldlr (C, D) and the LXR target gene Abcg1 (B) were measured by qPCR. Each bar represents the mean ± SEM of triplicate wells. Statistically significant differences by CD treatment for cells of a given genotype compared with the vehicle-treated group are represented by asterisks (* P

    Journal: Journal of Lipid Research

    Article Title: Cyclodextrin mediates rapid changes in lipid balance in Npc1−/− mice without carrying cholesterol through the bloodstream [S]

    doi: 10.1194/jlr.M028241

    Figure Lengend Snippet: Effects of HP-β-CD compared to SBE-β-CD in cultured cells from Npc1 −/− mice. Thioglycollate-elicited peritoneal (from 49-day-old mice) and hippocampal neurons (from neonatal mouse pups) were harvested from Npc1 +/+ and Npc1 −/− mice. These cells were treated in FBS-containing culture media with vehicle (saline), 0.3 mM HP-β-CD, or 0.3 mM SBE-β-CD. After 16 h, the cells were harvested for RNA, and the relative mRNA levels of SREBP2 target genes Hmg CoA Syn (A) and Ldlr (C, D) and the LXR target gene Abcg1 (B) were measured by qPCR. Each bar represents the mean ± SEM of triplicate wells. Statistically significant differences by CD treatment for cells of a given genotype compared with the vehicle-treated group are represented by asterisks (* P

    Article Snippet: For the CD dose-response experiments, cells were treated for 4 h with varying amounts of a 250 mM solution of HP-β-CD (H107; Sigma) made in PBS to yield final concentrations of 0.3, 0.75, and 1.5 mM.

    Techniques: Cell Culture, Mouse Assay, Real-time Polymerase Chain Reaction

    Cholesterol distribution in plasma and urine of Npc1 −/− mice after HP-β-CD injection. Plasma samples were collected from 49-day-old Npc1 +/+ and Npc1 −/− mice 0, 1, 2, 3, 6, and 12 h after an injection with HP-β-CD (4,000 mpk, sc) or equivalent volume of vehicle (saline). Plasmas were pooled (n = 2 pooled samples/group), and lipoprotein profiles were generated by FPLC. Lipoprotein profiles were plotted to allow comparison between (A) saline-treated Npc1 +/+ and Npc1 −/− mice; (B) the very early time points, 0–3 h, after HP-β-CD injection of Npc1 −/− mice; and (C) later time points, 6–12 h, after HP-β-CD injection of Npc1 −/− mice. Urine samples were collected from 49-day-old Npc1 +/+ and Npc1 −/− mice over a 48 h period after a HP-β-CD (4,000 mpk, sc) or vehicle (saline) injection. The total urinary cholesterol (D) values depict the mean ± SEM for 6 mice/group. A significant difference ( P

    Journal: Journal of Lipid Research

    Article Title: Cyclodextrin mediates rapid changes in lipid balance in Npc1−/− mice without carrying cholesterol through the bloodstream [S]

    doi: 10.1194/jlr.M028241

    Figure Lengend Snippet: Cholesterol distribution in plasma and urine of Npc1 −/− mice after HP-β-CD injection. Plasma samples were collected from 49-day-old Npc1 +/+ and Npc1 −/− mice 0, 1, 2, 3, 6, and 12 h after an injection with HP-β-CD (4,000 mpk, sc) or equivalent volume of vehicle (saline). Plasmas were pooled (n = 2 pooled samples/group), and lipoprotein profiles were generated by FPLC. Lipoprotein profiles were plotted to allow comparison between (A) saline-treated Npc1 +/+ and Npc1 −/− mice; (B) the very early time points, 0–3 h, after HP-β-CD injection of Npc1 −/− mice; and (C) later time points, 6–12 h, after HP-β-CD injection of Npc1 −/− mice. Urine samples were collected from 49-day-old Npc1 +/+ and Npc1 −/− mice over a 48 h period after a HP-β-CD (4,000 mpk, sc) or vehicle (saline) injection. The total urinary cholesterol (D) values depict the mean ± SEM for 6 mice/group. A significant difference ( P

    Article Snippet: For the CD dose-response experiments, cells were treated for 4 h with varying amounts of a 250 mM solution of HP-β-CD (H107; Sigma) made in PBS to yield final concentrations of 0.3, 0.75, and 1.5 mM.

    Techniques: Mouse Assay, Injection, Generated, Fast Protein Liquid Chromatography

    Acute effects of HP-β-CD in cultured cells from Npc1 −/− mice. Thioglycollate-elicited peritoneal macrophages (from 49-day-old mice) and hippocampal neurons (from neonatal mouse pups) were obtained from Npc1 +/+ and Npc1 −/− mice. These cells were treated in FBS-containing culture media with increasing amounts of HP-β-CD (0.3 mM to 1.5 mM) or an equivalent volume of vehicle (saline). After 4 h, the cells were harvested for RNA isolation, and the relative mRNA levels of LXR target genes, Idol (A) and Abcg1 (B), and SREBP2 target genes Hmg CoA Syn (C) and Ldlr (D) were determined by qPCR. Each bar represents the mean ± SEM of triplicate wells. A statistically significant difference ( P

    Journal: Journal of Lipid Research

    Article Title: Cyclodextrin mediates rapid changes in lipid balance in Npc1−/− mice without carrying cholesterol through the bloodstream [S]

    doi: 10.1194/jlr.M028241

    Figure Lengend Snippet: Acute effects of HP-β-CD in cultured cells from Npc1 −/− mice. Thioglycollate-elicited peritoneal macrophages (from 49-day-old mice) and hippocampal neurons (from neonatal mouse pups) were obtained from Npc1 +/+ and Npc1 −/− mice. These cells were treated in FBS-containing culture media with increasing amounts of HP-β-CD (0.3 mM to 1.5 mM) or an equivalent volume of vehicle (saline). After 4 h, the cells were harvested for RNA isolation, and the relative mRNA levels of LXR target genes, Idol (A) and Abcg1 (B), and SREBP2 target genes Hmg CoA Syn (C) and Ldlr (D) were determined by qPCR. Each bar represents the mean ± SEM of triplicate wells. A statistically significant difference ( P

    Article Snippet: For the CD dose-response experiments, cells were treated for 4 h with varying amounts of a 250 mM solution of HP-β-CD (H107; Sigma) made in PBS to yield final concentrations of 0.3, 0.75, and 1.5 mM.

    Techniques: Cell Culture, Mouse Assay, Isolation, Real-time Polymerase Chain Reaction

    Time course of HP-β-CD's effects on hepatic esterified and unesterified cholesterol concentrations and sterol-regulated mRNA levels in Npc1 −/− mice. Forty-nine-day-old Npc1 −/− mice were injected at time 0 with a 4,000 mpk bolus of HP-β-CD, and tissues were harvested after 0, 1, 2, 3, 6, or 12 h. Injection times were staggered so that all tissues were harvested at the same point during the light cycle (ZT 9). Concentrations of hepatic unesterified cholesterol (A) and cholesteryl ester (B) were determined at all-time points. Relative mRNA levels in liver for SREBP2 target genes Hmg CoA Syn (C) and Hmg CoA Red (E) and for LXR target genes Cyp7a1 (D) and Abca1 (F) were measured by qPCR using cyclophilin as the invariant housekeeping gene. Values represent the mean ± SEM for 4–6 mice per group. Statistically significant differences ( P

    Journal: Journal of Lipid Research

    Article Title: Cyclodextrin mediates rapid changes in lipid balance in Npc1−/− mice without carrying cholesterol through the bloodstream [S]

    doi: 10.1194/jlr.M028241

    Figure Lengend Snippet: Time course of HP-β-CD's effects on hepatic esterified and unesterified cholesterol concentrations and sterol-regulated mRNA levels in Npc1 −/− mice. Forty-nine-day-old Npc1 −/− mice were injected at time 0 with a 4,000 mpk bolus of HP-β-CD, and tissues were harvested after 0, 1, 2, 3, 6, or 12 h. Injection times were staggered so that all tissues were harvested at the same point during the light cycle (ZT 9). Concentrations of hepatic unesterified cholesterol (A) and cholesteryl ester (B) were determined at all-time points. Relative mRNA levels in liver for SREBP2 target genes Hmg CoA Syn (C) and Hmg CoA Red (E) and for LXR target genes Cyp7a1 (D) and Abca1 (F) were measured by qPCR using cyclophilin as the invariant housekeeping gene. Values represent the mean ± SEM for 4–6 mice per group. Statistically significant differences ( P

    Article Snippet: For the CD dose-response experiments, cells were treated for 4 h with varying amounts of a 250 mM solution of HP-β-CD (H107; Sigma) made in PBS to yield final concentrations of 0.3, 0.75, and 1.5 mM.

    Techniques: Mouse Assay, Injection, Real-time Polymerase Chain Reaction

    Time course of HP-β-CD's effects on tissue cholesterol content and sterol synthesis rates in Npc1 −/− mice . Forty-nine-day-old Npc1 −/− mice were injected at time 0 with a 4,000 mpk bolus of HP-β-CD, and tissues were harvested after 0, 3, 6, 9, or 12 h. Npc1 +/+ controls were studied 24 h after an injection of HP-β-CD. Organ weights were measured and expressed relative to the total body weight for liver (A) and spleen (B). Liver and spleen tissues were saponified to measure the total concentration of cholesterol (C, D) by GC and the rates of cholesterol synthesis (E, F) by the [ 3 H]water method. Each bar represents the mean ± SEM for 4–6 mice. Statistically significant differences ( P

    Journal: Journal of Lipid Research

    Article Title: Cyclodextrin mediates rapid changes in lipid balance in Npc1−/− mice without carrying cholesterol through the bloodstream [S]

    doi: 10.1194/jlr.M028241

    Figure Lengend Snippet: Time course of HP-β-CD's effects on tissue cholesterol content and sterol synthesis rates in Npc1 −/− mice . Forty-nine-day-old Npc1 −/− mice were injected at time 0 with a 4,000 mpk bolus of HP-β-CD, and tissues were harvested after 0, 3, 6, 9, or 12 h. Npc1 +/+ controls were studied 24 h after an injection of HP-β-CD. Organ weights were measured and expressed relative to the total body weight for liver (A) and spleen (B). Liver and spleen tissues were saponified to measure the total concentration of cholesterol (C, D) by GC and the rates of cholesterol synthesis (E, F) by the [ 3 H]water method. Each bar represents the mean ± SEM for 4–6 mice. Statistically significant differences ( P

    Article Snippet: For the CD dose-response experiments, cells were treated for 4 h with varying amounts of a 250 mM solution of HP-β-CD (H107; Sigma) made in PBS to yield final concentrations of 0.3, 0.75, and 1.5 mM.

    Techniques: Mouse Assay, Injection, Concentration Assay

    Tenovin-6 inhibits the initiation and progression of PEL, and extends the survival of animals in a murine PEL model. (A) Live imaging of PEL in mice treated with Tenovin-6 or vehicle control. Five weeks old NOD/SCID mice were injected with 10 7 BCBL-1 cells expressing the firefly luciferase protein. Beginning at day 2 post-inoculation, the mice were treated with Tenovin-6 (50 mg/kg) or vehicle control cyclodextrin (Cyclo) by daily intraperitoneal injection. At week 3, 4 and 6 post-inoculations, mice were examined for PEL development by live imaging using an IVIS Imaging System following intraperitoneal injection of D-luciferin (50 mg/kg). Data were analysed and presented as average radiance (photons/sec/cm 2 /sr). (B) Kaplan-Meier survival analysis of mice treated with Tenovin-6 (50 mg/kg) and vehicle control cyclodextrin as described in (A). (C) Inhibition of ascites formation by Tenovin-6 treatment in PEL. Ascites volumes from mice described in (A) were analysed. (D) Live imaging of PEL progression in mice treated with Tenovin-6 or vehicle control. The mice were treated with Tenovin-6 (100 mg/kg) or vehicle control cyclodextrin (Cyclo) by daily intraperitoneal injection after PEL had developed. At day 0, 8 and 16 post-treatments, mice were examined for PEL progression by live imaging as described in (A). (E) Inhibition of luciferase signal in mice by Tenovin-6 treatment as measured in (D). (F) Inhibition of weight gain of mice by Tenovin-6 during PEL progression. Two-tailed t-test was performed, statistical symbols “*”, “**” and “***” represent p-values

    Journal: The Journal of pathology

    Article Title: SIRT1 and AMPK pathways are essential for the proliferation and survival of primary effusion lymphoma cells

    doi: 10.1002/path.4905

    Figure Lengend Snippet: Tenovin-6 inhibits the initiation and progression of PEL, and extends the survival of animals in a murine PEL model. (A) Live imaging of PEL in mice treated with Tenovin-6 or vehicle control. Five weeks old NOD/SCID mice were injected with 10 7 BCBL-1 cells expressing the firefly luciferase protein. Beginning at day 2 post-inoculation, the mice were treated with Tenovin-6 (50 mg/kg) or vehicle control cyclodextrin (Cyclo) by daily intraperitoneal injection. At week 3, 4 and 6 post-inoculations, mice were examined for PEL development by live imaging using an IVIS Imaging System following intraperitoneal injection of D-luciferin (50 mg/kg). Data were analysed and presented as average radiance (photons/sec/cm 2 /sr). (B) Kaplan-Meier survival analysis of mice treated with Tenovin-6 (50 mg/kg) and vehicle control cyclodextrin as described in (A). (C) Inhibition of ascites formation by Tenovin-6 treatment in PEL. Ascites volumes from mice described in (A) were analysed. (D) Live imaging of PEL progression in mice treated with Tenovin-6 or vehicle control. The mice were treated with Tenovin-6 (100 mg/kg) or vehicle control cyclodextrin (Cyclo) by daily intraperitoneal injection after PEL had developed. At day 0, 8 and 16 post-treatments, mice were examined for PEL progression by live imaging as described in (A). (E) Inhibition of luciferase signal in mice by Tenovin-6 treatment as measured in (D). (F) Inhibition of weight gain of mice by Tenovin-6 during PEL progression. Two-tailed t-test was performed, statistical symbols “*”, “**” and “***” represent p-values

    Article Snippet: Cyclodextrin (#C0926) was from Sigma (St. Louis, MO).

    Techniques: Imaging, Mouse Assay, Injection, Expressing, Luciferase, Size-exclusion Chromatography, Inhibition, Two Tailed Test

    Antioxidant activity of DOPE in the β-carotene emulsion model system. ( a ): β-carotene absorbance inhibition ratio during 120 min incubation; ( b ): antioxidant activity expressed as IC 50. nDOPE-native dry olive pomace extract; hpDOPE-extract prepared with hydroxypropyl-β-cyclodextrin; ramDOPE-extract prepared with randomly methylated β-cyclodextrin; BHA: butylhydroxyanisol; IC 50 : half maximal inhibitory concentration. Columns (in Figure 3 b) marked with different letters belong to different statistical groups ( p

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Valorization of Olive Pomace-Based Nutraceuticals as Antioxidants in Chemical, Food, and Biological Models

    doi: 10.3390/molecules23082070

    Figure Lengend Snippet: Antioxidant activity of DOPE in the β-carotene emulsion model system. ( a ): β-carotene absorbance inhibition ratio during 120 min incubation; ( b ): antioxidant activity expressed as IC 50. nDOPE-native dry olive pomace extract; hpDOPE-extract prepared with hydroxypropyl-β-cyclodextrin; ramDOPE-extract prepared with randomly methylated β-cyclodextrin; BHA: butylhydroxyanisol; IC 50 : half maximal inhibitory concentration. Columns (in Figure 3 b) marked with different letters belong to different statistical groups ( p

    Article Snippet: Folin–Ciocalteu reagent, sodium carbonate, gallic acid, butyl-hydroxy anisole (BHA), phosphate buffer saline (PBS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), β-cyclodextrin (βCD) randomly-methylated β cyclodextrin (RAMEB), hydroxypropyl-β-cyclodextrin (HPβCD), γ cyclodextrin (γCD), 3′,6′-dihydroxyspiro[isobenzofuran-1[3H ],9′[9H ]-xanthen]-3-one (fluorescein), 2,2′-azobis-2-methyl-propanimidamide, dihydrochloride (AAPH), pBR322 Plasmid DNA from Escherichia coli RRI, malondialdehyde (MDA) tetrabutylammonium salt, 2-thiobarbituric acid, trichloroacetic acid, and human LDL (in PBS, pH 7.4, containing 0.01% of EDTA) were purchased from Sigma-Aldrich.

    Techniques: Antioxidant Activity Assay, Inhibition, Incubation, Methylation, Concentration Assay

    Oxidative stability of minced meat during storage at 4 °C and accelerate stability testing. ( a ): Oxidative stability during storage at 4 °C; ( b ): Inhibition of meat lipid peroxidation during accelerated testing in relation to concentration of antioxidant; ( c ): Antioxidant activity against meat lipid peroxidation during accelerated stability testing expressed as IC 50. nDOPE-native dry olive pomace extract; hpDOPE-extract prepared with hydroxypropyl-β-cyclodextrin; ramDOPE-extract prepared with randomly methylated β-cyclodextrin; BHA: butylhydroxyanisol; IC 50 : Half maximal inhibitory concentration. Different letters above data bars indicate significant difference ( p ≤ 0.05).

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Valorization of Olive Pomace-Based Nutraceuticals as Antioxidants in Chemical, Food, and Biological Models

    doi: 10.3390/molecules23082070

    Figure Lengend Snippet: Oxidative stability of minced meat during storage at 4 °C and accelerate stability testing. ( a ): Oxidative stability during storage at 4 °C; ( b ): Inhibition of meat lipid peroxidation during accelerated testing in relation to concentration of antioxidant; ( c ): Antioxidant activity against meat lipid peroxidation during accelerated stability testing expressed as IC 50. nDOPE-native dry olive pomace extract; hpDOPE-extract prepared with hydroxypropyl-β-cyclodextrin; ramDOPE-extract prepared with randomly methylated β-cyclodextrin; BHA: butylhydroxyanisol; IC 50 : Half maximal inhibitory concentration. Different letters above data bars indicate significant difference ( p ≤ 0.05).

    Article Snippet: Folin–Ciocalteu reagent, sodium carbonate, gallic acid, butyl-hydroxy anisole (BHA), phosphate buffer saline (PBS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), β-cyclodextrin (βCD) randomly-methylated β cyclodextrin (RAMEB), hydroxypropyl-β-cyclodextrin (HPβCD), γ cyclodextrin (γCD), 3′,6′-dihydroxyspiro[isobenzofuran-1[3H ],9′[9H ]-xanthen]-3-one (fluorescein), 2,2′-azobis-2-methyl-propanimidamide, dihydrochloride (AAPH), pBR322 Plasmid DNA from Escherichia coli RRI, malondialdehyde (MDA) tetrabutylammonium salt, 2-thiobarbituric acid, trichloroacetic acid, and human LDL (in PBS, pH 7.4, containing 0.01% of EDTA) were purchased from Sigma-Aldrich.

    Techniques: Inhibition, Concentration Assay, Antioxidant Activity Assay, Methylation

    Antioxidant activity of DOPE in oil. ( a ): Rancimat induction period; ( b ): Peroxide value of safflower oil during storage with 0.1% DOPE; ( c ): Peroxide value of safflower oil during storage with 0.3% DOPE. nDOPE-native dry olive pomace extract; hpDOPE-extract prepared with hydroxypropyl-β-cyclodextrin; ramDOPE-extract prepared with randomly methylated β-cyclodextrin; BHA: Butylhydroxyanisol; PG: Propyl-gallate.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Valorization of Olive Pomace-Based Nutraceuticals as Antioxidants in Chemical, Food, and Biological Models

    doi: 10.3390/molecules23082070

    Figure Lengend Snippet: Antioxidant activity of DOPE in oil. ( a ): Rancimat induction period; ( b ): Peroxide value of safflower oil during storage with 0.1% DOPE; ( c ): Peroxide value of safflower oil during storage with 0.3% DOPE. nDOPE-native dry olive pomace extract; hpDOPE-extract prepared with hydroxypropyl-β-cyclodextrin; ramDOPE-extract prepared with randomly methylated β-cyclodextrin; BHA: Butylhydroxyanisol; PG: Propyl-gallate.

    Article Snippet: Folin–Ciocalteu reagent, sodium carbonate, gallic acid, butyl-hydroxy anisole (BHA), phosphate buffer saline (PBS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), β-cyclodextrin (βCD) randomly-methylated β cyclodextrin (RAMEB), hydroxypropyl-β-cyclodextrin (HPβCD), γ cyclodextrin (γCD), 3′,6′-dihydroxyspiro[isobenzofuran-1[3H ],9′[9H ]-xanthen]-3-one (fluorescein), 2,2′-azobis-2-methyl-propanimidamide, dihydrochloride (AAPH), pBR322 Plasmid DNA from Escherichia coli RRI, malondialdehyde (MDA) tetrabutylammonium salt, 2-thiobarbituric acid, trichloroacetic acid, and human LDL (in PBS, pH 7.4, containing 0.01% of EDTA) were purchased from Sigma-Aldrich.

    Techniques: Antioxidant Activity Assay, Methylation

    Inhibition of plasmid DNA strand scission and antioxidant activity of DOPE in liposome membrane model. ( a ): supercoiled (native) form of plasmid pBR322 DNA in electrophoresis gel after incubation with AAPH with or without the presence of antioxidants; ( b ): antioxidant activity against antioxidant activity against plasmid DNA strand scission expressed as IC50; ( c ): antioxidant activity of DOPE in liposome membrane model system. nDOPE-native dry olive pomace extract; hpDOPE-extract prepared with hydroxypropyl-β-cyclodextrin; ramDOPE-extract prepared with randomly methylated β-cyclodextrin; BHA: butylhydroxyanisol; IC 50 : half maximal inhibitory concentration; TBARS: thiobarbituric acid reactive substances. Different letters above data bars indicate significant difference ( p ≤ 0.05).

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Valorization of Olive Pomace-Based Nutraceuticals as Antioxidants in Chemical, Food, and Biological Models

    doi: 10.3390/molecules23082070

    Figure Lengend Snippet: Inhibition of plasmid DNA strand scission and antioxidant activity of DOPE in liposome membrane model. ( a ): supercoiled (native) form of plasmid pBR322 DNA in electrophoresis gel after incubation with AAPH with or without the presence of antioxidants; ( b ): antioxidant activity against antioxidant activity against plasmid DNA strand scission expressed as IC50; ( c ): antioxidant activity of DOPE in liposome membrane model system. nDOPE-native dry olive pomace extract; hpDOPE-extract prepared with hydroxypropyl-β-cyclodextrin; ramDOPE-extract prepared with randomly methylated β-cyclodextrin; BHA: butylhydroxyanisol; IC 50 : half maximal inhibitory concentration; TBARS: thiobarbituric acid reactive substances. Different letters above data bars indicate significant difference ( p ≤ 0.05).

    Article Snippet: Folin–Ciocalteu reagent, sodium carbonate, gallic acid, butyl-hydroxy anisole (BHA), phosphate buffer saline (PBS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), β-cyclodextrin (βCD) randomly-methylated β cyclodextrin (RAMEB), hydroxypropyl-β-cyclodextrin (HPβCD), γ cyclodextrin (γCD), 3′,6′-dihydroxyspiro[isobenzofuran-1[3H ],9′[9H ]-xanthen]-3-one (fluorescein), 2,2′-azobis-2-methyl-propanimidamide, dihydrochloride (AAPH), pBR322 Plasmid DNA from Escherichia coli RRI, malondialdehyde (MDA) tetrabutylammonium salt, 2-thiobarbituric acid, trichloroacetic acid, and human LDL (in PBS, pH 7.4, containing 0.01% of EDTA) were purchased from Sigma-Aldrich.

    Techniques: Inhibition, Plasmid Preparation, Antioxidant Activity Assay, Electrophoresis, Incubation, Methylation, Concentration Assay