γ-33 p Search Results


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  • 99
    New England Biolabs γ 33 p atp
    γ 33 P Atp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer γ 33 p datp
    γ 33 P Datp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare γ 33 p datp
    γ 33 P Datp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    DuPont de Nemours γ 33 p atp
    Preservation of Vβ8.1 spectratypes during the T cell apoptosis phase from days 8 to 12 after LCMV infection. The representative CDR3 profiles from the PB of four acutely LCMV-infected mice 6–12 d after infection are displayed. Since the naive spectratypes invariably have a normal Gaussian distribution, we plot at the top of each column day 0 spectratypes from two naive control mice. To avoid traumatizing the mice at the time of LCMV infection, day 0 samples were not taken from the same mice analyzed on the subsequent days. The PCR products were subjected to run-off reactions using [γ- 33 <t>P]ATP-labeled</t> (Jβ1.1 and Jβ1.2) or fluorescent (Jβ1.3, Jβ1.5, and Jβ1.6) Jβ primers.
    γ 33 P Atp, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare γ 33 p atp
    (Previous page) Effect of KLF5 phosphorylation and CBP interaction. ( A ) In vitro phosphorylation of KLF5 by PKC. Left panel: colloidal blue stain. The expressed GST, GST–KLF5 and GST–KLF5 (1–238) proteins are indicated by an asterisk. The molecular weight markers are indicated. Right panel: autoradiogram of the [γ- 33 <t>P]ATP-labeled</t> GST–KLF5 and GST–KLF5 (1–238) (indicated by arrows). The exposure time was 12 h. ( B ) KLF5 (239–372) was not phosphorylated by PKC in vitro . Identical experiment to that in (A), except that GST–KLF5 (239–372) was used. Left panel: colloidal blue stain. The GST and the fusion protein are marked by an asterisk. Right panel: autoradiogram. GST–KLF5 (239–372) was not phosphorylated by PKC even after long exposure (24 h) of the X-ray film (arrow). ( C ) KLF5 phosphorylation enhances CBP interaction. Top panel: autoradiogram of the GST pull-down assay. The arrow indicates the labeled CBP that was pulled-down by the GST–KLF5 or GST–KLF5 (1–238) fusion proteins. Bottom panel: quantitation of the [ 35 S]CBP intensity by imaging analysis. The integrated density value is presented as a percentage, with the input intensity as 5%.
    γ 33 P Atp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HARTMANN ANALYTIC γ 33 p atp
    Comparison of western blots analyzed by immunostaining with GWD antibodies and by phosphor imaging. A, Starch granules were isolated from irradiated turions (1 d R) and incubated with [ γ - 33 <t>P]ATP.</t> Starch-associated proteins were extracted thereafter and analyzed after 2D-GE and western transfer by immunodetection using antibodies raised against GWD. Almost identical results were obtained after incubation with [ βγ - 33 P]ATP instead of [ γ - 33 P]ATP (data not shown). B, The same membranes as in A but were analyzed by phosphor imaging to detect radioactive labeled proteins. Starch granules were isolated from irradiated turions (1 d R) and incubated with [ γ - 33 P]ATP. C, The experiments were carried out as described in A, but randomized [ βγ - 33 P]ATP was used for incubation followed by phosphor imaging as described in B. D, Starch granules were isolated from irradiated turions (1 d R) and treated with acid phosphatase before being investigated by 2D-GE/western analysis using antibodies raised against GWD.
    γ 33 P Atp, supplied by HARTMANN ANALYTIC, used in various techniques. Bioz Stars score: 92/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    NEN Life Science γ 33 p atp
    Comparison of western blots analyzed by immunostaining with GWD antibodies and by phosphor imaging. A, Starch granules were isolated from irradiated turions (1 d R) and incubated with [ γ - 33 <t>P]ATP.</t> Starch-associated proteins were extracted thereafter and analyzed after 2D-GE and western transfer by immunodetection using antibodies raised against GWD. Almost identical results were obtained after incubation with [ βγ - 33 P]ATP instead of [ γ - 33 P]ATP (data not shown). B, The same membranes as in A but were analyzed by phosphor imaging to detect radioactive labeled proteins. Starch granules were isolated from irradiated turions (1 d R) and incubated with [ γ - 33 P]ATP. C, The experiments were carried out as described in A, but randomized [ βγ - 33 P]ATP was used for incubation followed by phosphor imaging as described in B. D, Starch granules were isolated from irradiated turions (1 d R) and treated with acid phosphatase before being investigated by 2D-GE/western analysis using antibodies raised against GWD.
    γ 33 P Atp, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer γ 33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Atp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer γ‑33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ‑33 P Atp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Valiant γ 33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Atp, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare γ 33 p
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer radioactive γ 33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    Radioactive γ 33 P Atp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer kbq γ 33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    Kbq γ 33 P Atp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DuPont de Nemours γ 33 p atp nen dupont
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Atp Nen Dupont, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GeneWorks γ 33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Atp, supplied by GeneWorks, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare γ 33 p atp 3000 mmol 1
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Atp 3000 Mmol 1, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer γ 33 p adenosine 5 triphosphate
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Adenosine 5 Triphosphate, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    American Radiolabeled Chemicals Inc γ 33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Atp, supplied by American Radiolabeled Chemicals Inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ICN Biomedicals γ 33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Atp, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer γ 33 p utp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Utp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Reaction Biology Corporation γ 33 p atp based enzymatic assays
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Atp Based Enzymatic Assays, supplied by Reaction Biology Corporation, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher invitrogen γ 33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    Invitrogen γ 33 P Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher γ 33 p labeled reverse primers
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    γ 33 P Labeled Reverse Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer gamma 33 p atp
    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 <t>P]ATP)</t> in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol
    Gamma 33 P Atp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Preservation of Vβ8.1 spectratypes during the T cell apoptosis phase from days 8 to 12 after LCMV infection. The representative CDR3 profiles from the PB of four acutely LCMV-infected mice 6–12 d after infection are displayed. Since the naive spectratypes invariably have a normal Gaussian distribution, we plot at the top of each column day 0 spectratypes from two naive control mice. To avoid traumatizing the mice at the time of LCMV infection, day 0 samples were not taken from the same mice analyzed on the subsequent days. The PCR products were subjected to run-off reactions using [γ- 33 P]ATP-labeled (Jβ1.1 and Jβ1.2) or fluorescent (Jβ1.3, Jβ1.5, and Jβ1.6) Jβ primers.

    Journal: The Journal of Experimental Medicine

    Article Title: Stability and Diversity of T Cell Receptor Repertoire Usage during Lymphocytic Choriomeningitis Virus Infection of Mice

    doi:

    Figure Lengend Snippet: Preservation of Vβ8.1 spectratypes during the T cell apoptosis phase from days 8 to 12 after LCMV infection. The representative CDR3 profiles from the PB of four acutely LCMV-infected mice 6–12 d after infection are displayed. Since the naive spectratypes invariably have a normal Gaussian distribution, we plot at the top of each column day 0 spectratypes from two naive control mice. To avoid traumatizing the mice at the time of LCMV infection, day 0 samples were not taken from the same mice analyzed on the subsequent days. The PCR products were subjected to run-off reactions using [γ- 33 P]ATP-labeled (Jβ1.1 and Jβ1.2) or fluorescent (Jβ1.3, Jβ1.5, and Jβ1.6) Jβ primers.

    Article Snippet: For 33 P-labeling, Jβ primers were labeled by using T4-polynucleotide kinase ( Boehringer Mannheim , Mannheim, Germany) and [γ-33 P]ATP ( DuPont-NEN ).

    Techniques: Preserving, Infection, Mouse Assay, Polymerase Chain Reaction, Labeling

    Host diversity in the broad spectrum of Vβ8.1 + T cell response as demonstrated by complete CDR3 size analysis within the Vβ8.1 + cells from day 8 LCMV-infected mice. CDR3 size patterns were obtained from day 8 LCMV-infected PBL from four C57BL/6 mice. Total RNAs from the PB were extracted and subjected to spectratyping as described in Materials and Methods. The PCR products were subjected to run-off reactions using [γ- 33 P]ATP-labeled ( A ) or fluorescent ( B ) Jβ primers. Jβ1.3 and Jβ1.6 were labeled with the fluorescent dye Ned; Jβ1.4 and Jβ2.5 with 6-Fam; Jβ1.5, Jβ2.3, and Jβ2.7 with Hex. Because primers labeled with different fluorophores give different fluorescent intensity, it is not appropriate to compare the relative fluorescent intensity between different fluorophore-labeled run-off products. The magnitude of fluorescent intensities is 6-Fam > Hex > Ned.

    Journal: The Journal of Experimental Medicine

    Article Title: Stability and Diversity of T Cell Receptor Repertoire Usage during Lymphocytic Choriomeningitis Virus Infection of Mice

    doi:

    Figure Lengend Snippet: Host diversity in the broad spectrum of Vβ8.1 + T cell response as demonstrated by complete CDR3 size analysis within the Vβ8.1 + cells from day 8 LCMV-infected mice. CDR3 size patterns were obtained from day 8 LCMV-infected PBL from four C57BL/6 mice. Total RNAs from the PB were extracted and subjected to spectratyping as described in Materials and Methods. The PCR products were subjected to run-off reactions using [γ- 33 P]ATP-labeled ( A ) or fluorescent ( B ) Jβ primers. Jβ1.3 and Jβ1.6 were labeled with the fluorescent dye Ned; Jβ1.4 and Jβ2.5 with 6-Fam; Jβ1.5, Jβ2.3, and Jβ2.7 with Hex. Because primers labeled with different fluorophores give different fluorescent intensity, it is not appropriate to compare the relative fluorescent intensity between different fluorophore-labeled run-off products. The magnitude of fluorescent intensities is 6-Fam > Hex > Ned.

    Article Snippet: For 33 P-labeling, Jβ primers were labeled by using T4-polynucleotide kinase ( Boehringer Mannheim , Mannheim, Germany) and [γ-33 P]ATP ( DuPont-NEN ).

    Techniques: Infection, Mouse Assay, Polymerase Chain Reaction, Labeling

    (Previous page) Effect of KLF5 phosphorylation and CBP interaction. ( A ) In vitro phosphorylation of KLF5 by PKC. Left panel: colloidal blue stain. The expressed GST, GST–KLF5 and GST–KLF5 (1–238) proteins are indicated by an asterisk. The molecular weight markers are indicated. Right panel: autoradiogram of the [γ- 33 P]ATP-labeled GST–KLF5 and GST–KLF5 (1–238) (indicated by arrows). The exposure time was 12 h. ( B ) KLF5 (239–372) was not phosphorylated by PKC in vitro . Identical experiment to that in (A), except that GST–KLF5 (239–372) was used. Left panel: colloidal blue stain. The GST and the fusion protein are marked by an asterisk. Right panel: autoradiogram. GST–KLF5 (239–372) was not phosphorylated by PKC even after long exposure (24 h) of the X-ray film (arrow). ( C ) KLF5 phosphorylation enhances CBP interaction. Top panel: autoradiogram of the GST pull-down assay. The arrow indicates the labeled CBP that was pulled-down by the GST–KLF5 or GST–KLF5 (1–238) fusion proteins. Bottom panel: quantitation of the [ 35 S]CBP intensity by imaging analysis. The integrated density value is presented as a percentage, with the input intensity as 5%.

    Journal: Nucleic Acids Research

    Article Title: Phosphorylation of Kruppel-like factor 5 (KLF5/IKLF) at the CBP interaction region enhances its transactivation function

    doi:

    Figure Lengend Snippet: (Previous page) Effect of KLF5 phosphorylation and CBP interaction. ( A ) In vitro phosphorylation of KLF5 by PKC. Left panel: colloidal blue stain. The expressed GST, GST–KLF5 and GST–KLF5 (1–238) proteins are indicated by an asterisk. The molecular weight markers are indicated. Right panel: autoradiogram of the [γ- 33 P]ATP-labeled GST–KLF5 and GST–KLF5 (1–238) (indicated by arrows). The exposure time was 12 h. ( B ) KLF5 (239–372) was not phosphorylated by PKC in vitro . Identical experiment to that in (A), except that GST–KLF5 (239–372) was used. Left panel: colloidal blue stain. The GST and the fusion protein are marked by an asterisk. Right panel: autoradiogram. GST–KLF5 (239–372) was not phosphorylated by PKC even after long exposure (24 h) of the X-ray film (arrow). ( C ) KLF5 phosphorylation enhances CBP interaction. Top panel: autoradiogram of the GST pull-down assay. The arrow indicates the labeled CBP that was pulled-down by the GST–KLF5 or GST–KLF5 (1–238) fusion proteins. Bottom panel: quantitation of the [ 35 S]CBP intensity by imaging analysis. The integrated density value is presented as a percentage, with the input intensity as 5%.

    Article Snippet: [35 S]Methionine, S-linkage glutathione–Sepharose beads, [γ-33 P]ATP and [3 H]acetyl CoA were purchased from Amersham Pharmacia Biotech, and [14 C]chloramphenicol from NEN Life Science Products (Boston, MA).

    Techniques: Polyacrylamide Gel Electrophoresis, In Vitro, Staining, Molecular Weight, Labeling, Pull Down Assay, Quantitation Assay, Imaging

    Comparison of western blots analyzed by immunostaining with GWD antibodies and by phosphor imaging. A, Starch granules were isolated from irradiated turions (1 d R) and incubated with [ γ - 33 P]ATP. Starch-associated proteins were extracted thereafter and analyzed after 2D-GE and western transfer by immunodetection using antibodies raised against GWD. Almost identical results were obtained after incubation with [ βγ - 33 P]ATP instead of [ γ - 33 P]ATP (data not shown). B, The same membranes as in A but were analyzed by phosphor imaging to detect radioactive labeled proteins. Starch granules were isolated from irradiated turions (1 d R) and incubated with [ γ - 33 P]ATP. C, The experiments were carried out as described in A, but randomized [ βγ - 33 P]ATP was used for incubation followed by phosphor imaging as described in B. D, Starch granules were isolated from irradiated turions (1 d R) and treated with acid phosphatase before being investigated by 2D-GE/western analysis using antibodies raised against GWD.

    Journal: Plant Physiology

    Article Title: Light Induces Phosphorylation of Glucan Water Dikinase, Which Precedes Starch Degradation in Turions of the Duckweed Spirodela polyrhiza 1 1 2

    doi: 10.1104/pp.103.036236

    Figure Lengend Snippet: Comparison of western blots analyzed by immunostaining with GWD antibodies and by phosphor imaging. A, Starch granules were isolated from irradiated turions (1 d R) and incubated with [ γ - 33 P]ATP. Starch-associated proteins were extracted thereafter and analyzed after 2D-GE and western transfer by immunodetection using antibodies raised against GWD. Almost identical results were obtained after incubation with [ βγ - 33 P]ATP instead of [ γ - 33 P]ATP (data not shown). B, The same membranes as in A but were analyzed by phosphor imaging to detect radioactive labeled proteins. Starch granules were isolated from irradiated turions (1 d R) and incubated with [ γ - 33 P]ATP. C, The experiments were carried out as described in A, but randomized [ βγ - 33 P]ATP was used for incubation followed by phosphor imaging as described in B. D, Starch granules were isolated from irradiated turions (1 d R) and treated with acid phosphatase before being investigated by 2D-GE/western analysis using antibodies raised against GWD.

    Article Snippet: For the incubation of starch granules with radioactive ATP, two different labeled ATP preparations were used: [ γ -33 P]ATP (Hartmann Analytic, Braunschweig, Germany) and a mixture of [ β -33 P]ATP and [ γ -33 P]ATP (designated as γ -ATP and γβ -ATP) obtained by enzymatic randomization with myokinase and pyruvate kinase ( ).

    Techniques: Western Blot, Immunostaining, Imaging, Isolation, Irradiation, Incubation, Immunodetection, Labeling

    Labeling of starch granules with [ βγ - 33 P]ATP. Starch granules were isolated from irradiated (1 d R) or dark-adapted (D) turions and incubated with enzymatic randomized [ βγ - 33 P]ATP. Starch-associated proteins were removed, and the granules were thoroughly washed, suspended in water, and measured in a scintillation counter. As control, starch granules from irradiated turions were incubated with [ γ - 33 P]ATP, which resulted in 400 cpm.

    Journal: Plant Physiology

    Article Title: Light Induces Phosphorylation of Glucan Water Dikinase, Which Precedes Starch Degradation in Turions of the Duckweed Spirodela polyrhiza 1 1 2

    doi: 10.1104/pp.103.036236

    Figure Lengend Snippet: Labeling of starch granules with [ βγ - 33 P]ATP. Starch granules were isolated from irradiated (1 d R) or dark-adapted (D) turions and incubated with enzymatic randomized [ βγ - 33 P]ATP. Starch-associated proteins were removed, and the granules were thoroughly washed, suspended in water, and measured in a scintillation counter. As control, starch granules from irradiated turions were incubated with [ γ - 33 P]ATP, which resulted in 400 cpm.

    Article Snippet: For the incubation of starch granules with radioactive ATP, two different labeled ATP preparations were used: [ γ -33 P]ATP (Hartmann Analytic, Braunschweig, Germany) and a mixture of [ β -33 P]ATP and [ γ -33 P]ATP (designated as γ -ATP and γβ -ATP) obtained by enzymatic randomization with myokinase and pyruvate kinase ( ).

    Techniques: Labeling, Isolation, Irradiation, Incubation

    Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 P]ATP) in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol

    Journal: The Journal of Biological Chemistry

    Article Title: Characterization of the AtsR Hybrid Sensor Kinase Phosphorelay Pathway and Identification of Its Response Regulator in Burkholderia cenocepacia *

    doi: 10.1074/jbc.M113.489914

    Figure Lengend Snippet: Kinetics of phosphotransfer from AtsR to AtsT. A , 5 μmol of AtsR were preincubated with 5 μCi ([γ- 33 P]ATP) in a standard phosphorylation mixture (100 m m Tris-HCl, pH 8, 50 m m KCl, 5 m m MgCl 2 , 1 m m DTT) for 15 min, and then 5 μmol

    Article Snippet: For autophosphorylation and phosphotransfer reactions, 5 μmol of each protein was added to the phosphorylation buffer containing 50 m m Tris-HCl, pH 8.0, 50 m m KCl, 5 m m MgCl2 , 1 m m DTT, and 5 μCi of [γ-33 P]ATP (specific activity of 3000 Ci/mmol; 3.3 μ m stock solution) (PerkinElmer Life Sciences) in a final volume of 10 μl.

    Techniques: