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    PerkinElmer γ 32 p atp
    Tau phosphorylation by GSK-3B . A) SDS-PAGE analysis of tau protein incubated for 20 h in the absence (lanes 1, 4, 7, 10, and 13) or the presence of 0.006 U GSK-3β per pmol tau (lanes 2, 5, 8, 11, 14) or 0.018 U GSK-3β per pmol tau (lanes 3, 6, 9, 12, and 15). A definite band shift in the migration of phosphorylated tau can be detected with increasing time and kinase concentration. B) The amount of γ- 32 P incorporation over time using 0.018 U GSK-3β per pmol tau (open circles, right y-axis) is compared to the SDS-PAGE analysis in Panel A (open squares, left y-axis). Lines are drawn through the points to ease comparison. Data represents a single trial.
    γ 32 P Atp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 14779 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tau phosphorylation by GSK-3B . A) SDS-PAGE analysis of tau protein incubated for 20 h in the absence (lanes 1, 4, 7, 10, and 13) or the presence of 0.006 U GSK-3β per pmol tau (lanes 2, 5, 8, 11, 14) or 0.018 U GSK-3β per pmol tau (lanes 3, 6, 9, 12, and 15). A definite band shift in the migration of phosphorylated tau can be detected with increasing time and kinase concentration. B) The amount of γ- 32 P incorporation over time using 0.018 U GSK-3β per pmol tau (open circles, right y-axis) is compared to the SDS-PAGE analysis in Panel A (open squares, left y-axis). Lines are drawn through the points to ease comparison. Data represents a single trial.

    Journal: Molecular Neurodegeneration

    Article Title: Tau phosphorylation by GSK-3? promotes tangle-like filament morphology

    doi: 10.1186/1750-1326-2-12

    Figure Lengend Snippet: Tau phosphorylation by GSK-3B . A) SDS-PAGE analysis of tau protein incubated for 20 h in the absence (lanes 1, 4, 7, 10, and 13) or the presence of 0.006 U GSK-3β per pmol tau (lanes 2, 5, 8, 11, 14) or 0.018 U GSK-3β per pmol tau (lanes 3, 6, 9, 12, and 15). A definite band shift in the migration of phosphorylated tau can be detected with increasing time and kinase concentration. B) The amount of γ- 32 P incorporation over time using 0.018 U GSK-3β per pmol tau (open circles, right y-axis) is compared to the SDS-PAGE analysis in Panel A (open squares, left y-axis). Lines are drawn through the points to ease comparison. Data represents a single trial.

    Article Snippet: Determination of phosphate incorporation Tau protein at a final concentration of 16 μM was incubated with 0.018 U GSK-3β per pmol tau in buffer containing 40 mM HEPES, pH 7.64, 5 mM EGTA, 3 mM MgCl2 , and 2 mM ATP containing 10 μCi [γ-32 P] labeled ATP (Specific activity: 3000 Ci/mmol) (Perkin-Elmer, Boston, MA) for 20 h at 30°C.

    Techniques: SDS Page, Incubation, Electrophoretic Mobility Shift Assay, Migration, Concentration Assay