β-tubulin Search Results


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  • 99
    Millipore monoclonal anti beta tubulin antibody
    Monoclonal Anti Beta Tubulin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti beta tubulin antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti beta tubulin antibody - by Bioz Stars, 2021-03
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    99
    Millipore α tubulin
    Acute expression of F3-T3 fusion induces peroxisome biogenesis through phosphorylation of Y122 of PIN4 a , Representative confocal microphotographs (maximum intensity) of immunofluorescence staining for total PIN4 (PIN4, red, left panel) and pY122-PIN4 (pPIN4, red, middle panel) in vector and HA expressing F3-T3. Right panels show higher magnification of dotted boxes. Nuclei were counterstained with DAPI (blue). Experiment was repeated independently two times with similar results. b , Maximum intensity of confocal images of double immunofluorescence staining for FGFR3 (green, middle panel) and pY122-PIN4 (red, right panel) in HA-F3-T3. Arrows indicate protein co-localization. Experiment was repeated independently two times with similar results. c , Co-immunoprecipitation from H1299 cells using PIN4 antibody. Endogenous PIN4 immunocomplexes and input (WCL) were analyzed by western blot using the indicated antibodies. Input is 10% for PEX1, PEX6, SUN2 and NUP214; 5% for SEC16A and DHX30; 2% for PIN4. d , Western blot analysis of co-immunoprecipitation of exogenous FLAG-PEX1 in HA-F3-T3. WCL: 1% for PIN4 and 10% for PEX1 and PEX6. Experiment was repeated independently four times with similar results. e , qRT-PCR for PEX1 in HA-F3-T3 and HA-vector. Data are Mean±s.d. (n=3 technical replicates) of one representative experiment out of three independent experiments performed in triplicate. f , Western blot analysis of PEX1 expression in HA transduced with F3-T3, F3-T3-K508M or the empty vector. β-actin is shown as loading control. Experiment was repeated independently three times with similar results. g , Time course analysis of F3-T3 expression in HA by western blot. <t>α-tubulin</t> is shown as loading control. Experiment was repeated independently two times with similar results. h , Quantification of protein biosynthesis by OPP incorporation measured by high-content fluorescent microscopy in HA reconstituted with PIN4-WT or PIN4-Y122F after silencing of the endogenous PIN4 and acutely transduced with F3-T3 or vector. Representative bar plots (n=4 technical replicates) from one out of three independent experiments. P: *
    α Tubulin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α tubulin/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α tubulin - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    99
    Millipore anti α tubulin
    Acute expression of F3-T3 fusion induces peroxisome biogenesis through phosphorylation of Y122 of PIN4 a , Representative confocal microphotographs (maximum intensity) of immunofluorescence staining for total PIN4 (PIN4, red, left panel) and pY122-PIN4 (pPIN4, red, middle panel) in vector and HA expressing F3-T3. Right panels show higher magnification of dotted boxes. Nuclei were counterstained with DAPI (blue). Experiment was repeated independently two times with similar results. b , Maximum intensity of confocal images of double immunofluorescence staining for FGFR3 (green, middle panel) and pY122-PIN4 (red, right panel) in HA-F3-T3. Arrows indicate protein co-localization. Experiment was repeated independently two times with similar results. c , Co-immunoprecipitation from H1299 cells using PIN4 antibody. Endogenous PIN4 immunocomplexes and input (WCL) were analyzed by western blot using the indicated antibodies. Input is 10% for PEX1, PEX6, SUN2 and NUP214; 5% for SEC16A and DHX30; 2% for PIN4. d , Western blot analysis of co-immunoprecipitation of exogenous FLAG-PEX1 in HA-F3-T3. WCL: 1% for PIN4 and 10% for PEX1 and PEX6. Experiment was repeated independently four times with similar results. e , qRT-PCR for PEX1 in HA-F3-T3 and HA-vector. Data are Mean±s.d. (n=3 technical replicates) of one representative experiment out of three independent experiments performed in triplicate. f , Western blot analysis of PEX1 expression in HA transduced with F3-T3, F3-T3-K508M or the empty vector. β-actin is shown as loading control. Experiment was repeated independently three times with similar results. g , Time course analysis of F3-T3 expression in HA by western blot. <t>α-tubulin</t> is shown as loading control. Experiment was repeated independently two times with similar results. h , Quantification of protein biosynthesis by OPP incorporation measured by high-content fluorescent microscopy in HA reconstituted with PIN4-WT or PIN4-Y122F after silencing of the endogenous PIN4 and acutely transduced with F3-T3 or vector. Representative bar plots (n=4 technical replicates) from one out of three independent experiments. P: *
    Anti α Tubulin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti α tubulin/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti α tubulin - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

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    Tubulin is the major constituent of microtubules It binds two moles of GTP one at an exchangeable site on the beta chain and one at a non exchangeable site on
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    N/A
    Purified Rabbit Polyclonal Antibody Pab Rabbit Ig
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    N/A
    Rabbit Polyclonal to tubulin Beta Isotype Note IgG Host Note Rabbit Conjugation Note Unconjugated Reactivity Note Human Mouse Rat Application Note WB IHC P P ELISA
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    N/A
    The beta Tubulin Antibody from Novus Biologicals is a rabbit polyclonal antibody to beta Tubulin This antibody reacts with human mouse The beta Tubulin Antibody has been validated for the
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    Image Search Results


    Acute expression of F3-T3 fusion induces peroxisome biogenesis through phosphorylation of Y122 of PIN4 a , Representative confocal microphotographs (maximum intensity) of immunofluorescence staining for total PIN4 (PIN4, red, left panel) and pY122-PIN4 (pPIN4, red, middle panel) in vector and HA expressing F3-T3. Right panels show higher magnification of dotted boxes. Nuclei were counterstained with DAPI (blue). Experiment was repeated independently two times with similar results. b , Maximum intensity of confocal images of double immunofluorescence staining for FGFR3 (green, middle panel) and pY122-PIN4 (red, right panel) in HA-F3-T3. Arrows indicate protein co-localization. Experiment was repeated independently two times with similar results. c , Co-immunoprecipitation from H1299 cells using PIN4 antibody. Endogenous PIN4 immunocomplexes and input (WCL) were analyzed by western blot using the indicated antibodies. Input is 10% for PEX1, PEX6, SUN2 and NUP214; 5% for SEC16A and DHX30; 2% for PIN4. d , Western blot analysis of co-immunoprecipitation of exogenous FLAG-PEX1 in HA-F3-T3. WCL: 1% for PIN4 and 10% for PEX1 and PEX6. Experiment was repeated independently four times with similar results. e , qRT-PCR for PEX1 in HA-F3-T3 and HA-vector. Data are Mean±s.d. (n=3 technical replicates) of one representative experiment out of three independent experiments performed in triplicate. f , Western blot analysis of PEX1 expression in HA transduced with F3-T3, F3-T3-K508M or the empty vector. β-actin is shown as loading control. Experiment was repeated independently three times with similar results. g , Time course analysis of F3-T3 expression in HA by western blot. α-tubulin is shown as loading control. Experiment was repeated independently two times with similar results. h , Quantification of protein biosynthesis by OPP incorporation measured by high-content fluorescent microscopy in HA reconstituted with PIN4-WT or PIN4-Y122F after silencing of the endogenous PIN4 and acutely transduced with F3-T3 or vector. Representative bar plots (n=4 technical replicates) from one out of three independent experiments. P: *

    Journal: Nature

    Article Title: A metabolic function of FGFR3-TACC3 gene fusions in cancer

    doi: 10.1038/nature25171

    Figure Lengend Snippet: Acute expression of F3-T3 fusion induces peroxisome biogenesis through phosphorylation of Y122 of PIN4 a , Representative confocal microphotographs (maximum intensity) of immunofluorescence staining for total PIN4 (PIN4, red, left panel) and pY122-PIN4 (pPIN4, red, middle panel) in vector and HA expressing F3-T3. Right panels show higher magnification of dotted boxes. Nuclei were counterstained with DAPI (blue). Experiment was repeated independently two times with similar results. b , Maximum intensity of confocal images of double immunofluorescence staining for FGFR3 (green, middle panel) and pY122-PIN4 (red, right panel) in HA-F3-T3. Arrows indicate protein co-localization. Experiment was repeated independently two times with similar results. c , Co-immunoprecipitation from H1299 cells using PIN4 antibody. Endogenous PIN4 immunocomplexes and input (WCL) were analyzed by western blot using the indicated antibodies. Input is 10% for PEX1, PEX6, SUN2 and NUP214; 5% for SEC16A and DHX30; 2% for PIN4. d , Western blot analysis of co-immunoprecipitation of exogenous FLAG-PEX1 in HA-F3-T3. WCL: 1% for PIN4 and 10% for PEX1 and PEX6. Experiment was repeated independently four times with similar results. e , qRT-PCR for PEX1 in HA-F3-T3 and HA-vector. Data are Mean±s.d. (n=3 technical replicates) of one representative experiment out of three independent experiments performed in triplicate. f , Western blot analysis of PEX1 expression in HA transduced with F3-T3, F3-T3-K508M or the empty vector. β-actin is shown as loading control. Experiment was repeated independently three times with similar results. g , Time course analysis of F3-T3 expression in HA by western blot. α-tubulin is shown as loading control. Experiment was repeated independently two times with similar results. h , Quantification of protein biosynthesis by OPP incorporation measured by high-content fluorescent microscopy in HA reconstituted with PIN4-WT or PIN4-Y122F after silencing of the endogenous PIN4 and acutely transduced with F3-T3 or vector. Representative bar plots (n=4 technical replicates) from one out of three independent experiments. P: *

    Article Snippet: Antibodies and concentrations are: FGFR3 1:1000 (Santa Cruz, B-9, sc-13121), PIN4 1:1000 (Abcam, ab155283), PKM2 1:1000 (Cell Signaling, #3198), DLG3/SAP102 1:1000 (Cell Signaling, #3733), GOLGIN84 1:2000 (Santa Cruz, H-283, sc-134704); C1ORF50 1:1000 (Novus Biologicals, NBP1-81053), HGS 1:1000 (Abcam, ab72053), FAK 1:1000 (Cell Signaling, 3285), Paxillin 1:1000 (BD Transduction, 610051), PGC1α 1:500 (Santa Cruz, H300, sc-13067), PGC1α 1:1000 (Novus Biological, NBP104676), ESRRG 1:500 (Abcam, ab128930), ESRRG 1:500 (R7D, PP-H6812000) p-FRS2 1:1000 (Cell Signaling, 3861), FRS2 1:1000 (Santa Cruz, sc-8318), p-STAT3 1:1000 (Cell Signaling, #9131), STAT3 1:1000 (Santa Cruz, C-20 sc-482,), p-AKT 1:1000 (Cell Signaling, #4060), AKT 1:1000 (Cell Signaling, #9272), p-ERK1/2 1:1000 (Cell Signaling, #4370), ERK1/2 1:1000 (Cell Signaling, #9102), β-actin 1:2000 (Sigma, A5441), PEX1 1:500 (BD Biosciences #611719), PEX6 1:500 (Stress Marq, #SMC-470), NUP214 1:500 (Abcam #ab70497), SEC16A 1:500 (Abcam #ab70722), DHX30 1:500 (Novus Biologicals, NBP1-26203), SUN-2 1:500 (Abcam #ab124916), FLAG 1:1000 (Abcam ab1162), Retinoblastoma 1:1000 (BD Pharmingen 554136), α-tubulin 1:2000 (Sigma, T5168), total OXPHOS 1:1000 (Abcam, #ab110411), MTCO1 1:1000 (Abcam, #ab14705).

    Techniques: Expressing, Immunofluorescence, Staining, Plasmid Preparation, Double Immunofluorescence Staining, Immunoprecipitation, Western Blot, Quantitative RT-PCR, Transduction, Microscopy

    Functional analysis of tyrosine phosphorylation of F3-T3 kinase substrates a , Western blot analysis of phosphotyrosine immunoprecipitation of mGSC-F3-T3-sh TP53 and mGSC-HRAS-12V-sh TP53 using PIN4 antibody. F3-T3 and RAS-12V expression are shown. α-tubulin is shown as loading control b , Microphotographs of immunofluorescence using the pY122-PIN4 specific antibody (red, upper panels) in tumors from mGSC-F3-T3-sh TP53 and mGSC-HRAS-12V-sh TP53. Nuclei were counterstained with DAPI (blue, lower panels). Experiment was repeated independently two times with similar results. c , Representative microphotographs of pY122-PIN4 immunofluorescence in F3-T3-positive (upper panels) and F3-T3-negative (lower panels) GBM (green). Right panels show higher magnification of pY122-PIN4-DAPI co-staining depicting cytoplasmic localization of pY122-PIN4. DAPI staining of nuclei is shown as an indication of cellular density (middle panels). d , Analysis of OCR of HA-F3-T3 transduced with WT, or the unphosphorylable mutant (Y/A) of GOLGIN84, C1ORF50 and DLG3. HA-vector are included as control. Data are Mean±s.d. (n=5 technical replicates) of one representative experiment out of two independent experiments performed in triplicate with similar results. P

    Journal: Nature

    Article Title: A metabolic function of FGFR3-TACC3 gene fusions in cancer

    doi: 10.1038/nature25171

    Figure Lengend Snippet: Functional analysis of tyrosine phosphorylation of F3-T3 kinase substrates a , Western blot analysis of phosphotyrosine immunoprecipitation of mGSC-F3-T3-sh TP53 and mGSC-HRAS-12V-sh TP53 using PIN4 antibody. F3-T3 and RAS-12V expression are shown. α-tubulin is shown as loading control b , Microphotographs of immunofluorescence using the pY122-PIN4 specific antibody (red, upper panels) in tumors from mGSC-F3-T3-sh TP53 and mGSC-HRAS-12V-sh TP53. Nuclei were counterstained with DAPI (blue, lower panels). Experiment was repeated independently two times with similar results. c , Representative microphotographs of pY122-PIN4 immunofluorescence in F3-T3-positive (upper panels) and F3-T3-negative (lower panels) GBM (green). Right panels show higher magnification of pY122-PIN4-DAPI co-staining depicting cytoplasmic localization of pY122-PIN4. DAPI staining of nuclei is shown as an indication of cellular density (middle panels). d , Analysis of OCR of HA-F3-T3 transduced with WT, or the unphosphorylable mutant (Y/A) of GOLGIN84, C1ORF50 and DLG3. HA-vector are included as control. Data are Mean±s.d. (n=5 technical replicates) of one representative experiment out of two independent experiments performed in triplicate with similar results. P

    Article Snippet: Antibodies and concentrations are: FGFR3 1:1000 (Santa Cruz, B-9, sc-13121), PIN4 1:1000 (Abcam, ab155283), PKM2 1:1000 (Cell Signaling, #3198), DLG3/SAP102 1:1000 (Cell Signaling, #3733), GOLGIN84 1:2000 (Santa Cruz, H-283, sc-134704); C1ORF50 1:1000 (Novus Biologicals, NBP1-81053), HGS 1:1000 (Abcam, ab72053), FAK 1:1000 (Cell Signaling, 3285), Paxillin 1:1000 (BD Transduction, 610051), PGC1α 1:500 (Santa Cruz, H300, sc-13067), PGC1α 1:1000 (Novus Biological, NBP104676), ESRRG 1:500 (Abcam, ab128930), ESRRG 1:500 (R7D, PP-H6812000) p-FRS2 1:1000 (Cell Signaling, 3861), FRS2 1:1000 (Santa Cruz, sc-8318), p-STAT3 1:1000 (Cell Signaling, #9131), STAT3 1:1000 (Santa Cruz, C-20 sc-482,), p-AKT 1:1000 (Cell Signaling, #4060), AKT 1:1000 (Cell Signaling, #9272), p-ERK1/2 1:1000 (Cell Signaling, #4370), ERK1/2 1:1000 (Cell Signaling, #9102), β-actin 1:2000 (Sigma, A5441), PEX1 1:500 (BD Biosciences #611719), PEX6 1:500 (Stress Marq, #SMC-470), NUP214 1:500 (Abcam #ab70497), SEC16A 1:500 (Abcam #ab70722), DHX30 1:500 (Novus Biologicals, NBP1-26203), SUN-2 1:500 (Abcam #ab124916), FLAG 1:1000 (Abcam ab1162), Retinoblastoma 1:1000 (BD Pharmingen 554136), α-tubulin 1:2000 (Sigma, T5168), total OXPHOS 1:1000 (Abcam, #ab110411), MTCO1 1:1000 (Abcam, #ab14705).

    Techniques: Functional Assay, Western Blot, Immunoprecipitation, Expressing, Immunofluorescence, Staining, Transduction, Mutagenesis, Plasmid Preparation

    F3-T3 induces sensitivity to inhibitors of mitochondrial metabolism a , Immunoblot analysis using the FGFR3 antibody in HA-vector, HA-F3-T3 or HA-F3-T3-K508M. α-tubulin is shown as loading control. Experiment was repeated five times with similar results. b , OCR of GSC1123 harboring F3-T3 in the presence or absence of AZD4547. Data are Mean±s.d. (n=6 technical replicates) of one representative experiment out of two independent experiments. P:

    Journal: Nature

    Article Title: A metabolic function of FGFR3-TACC3 gene fusions in cancer

    doi: 10.1038/nature25171

    Figure Lengend Snippet: F3-T3 induces sensitivity to inhibitors of mitochondrial metabolism a , Immunoblot analysis using the FGFR3 antibody in HA-vector, HA-F3-T3 or HA-F3-T3-K508M. α-tubulin is shown as loading control. Experiment was repeated five times with similar results. b , OCR of GSC1123 harboring F3-T3 in the presence or absence of AZD4547. Data are Mean±s.d. (n=6 technical replicates) of one representative experiment out of two independent experiments. P:

    Article Snippet: Antibodies and concentrations are: FGFR3 1:1000 (Santa Cruz, B-9, sc-13121), PIN4 1:1000 (Abcam, ab155283), PKM2 1:1000 (Cell Signaling, #3198), DLG3/SAP102 1:1000 (Cell Signaling, #3733), GOLGIN84 1:2000 (Santa Cruz, H-283, sc-134704); C1ORF50 1:1000 (Novus Biologicals, NBP1-81053), HGS 1:1000 (Abcam, ab72053), FAK 1:1000 (Cell Signaling, 3285), Paxillin 1:1000 (BD Transduction, 610051), PGC1α 1:500 (Santa Cruz, H300, sc-13067), PGC1α 1:1000 (Novus Biological, NBP104676), ESRRG 1:500 (Abcam, ab128930), ESRRG 1:500 (R7D, PP-H6812000) p-FRS2 1:1000 (Cell Signaling, 3861), FRS2 1:1000 (Santa Cruz, sc-8318), p-STAT3 1:1000 (Cell Signaling, #9131), STAT3 1:1000 (Santa Cruz, C-20 sc-482,), p-AKT 1:1000 (Cell Signaling, #4060), AKT 1:1000 (Cell Signaling, #9272), p-ERK1/2 1:1000 (Cell Signaling, #4370), ERK1/2 1:1000 (Cell Signaling, #9102), β-actin 1:2000 (Sigma, A5441), PEX1 1:500 (BD Biosciences #611719), PEX6 1:500 (Stress Marq, #SMC-470), NUP214 1:500 (Abcam #ab70497), SEC16A 1:500 (Abcam #ab70722), DHX30 1:500 (Novus Biologicals, NBP1-26203), SUN-2 1:500 (Abcam #ab124916), FLAG 1:1000 (Abcam ab1162), Retinoblastoma 1:1000 (BD Pharmingen 554136), α-tubulin 1:2000 (Sigma, T5168), total OXPHOS 1:1000 (Abcam, #ab110411), MTCO1 1:1000 (Abcam, #ab14705).

    Techniques: Plasmid Preparation