β-mercaptoethanol Millipore Search Results


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  • 95
    Millipore β mercaptoethanol spermine
    β Mercaptoethanol Spermine, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore β mercaptoethanol β me
    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; <t>β-ME,</t> <t>β-mercaptoethanol;</t> dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC
    β Mercaptoethanol β Me, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Merck KGaA β mercaptoethanol
    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; <t>β-ME,</t> <t>β-mercaptoethanol;</t> dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC
    β Mercaptoethanol, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 97/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore β mercaptoethanol pbst
    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; <t>β-ME,</t> <t>β-mercaptoethanol;</t> dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC
    β Mercaptoethanol Pbst, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore β mercaptoethanol βme
    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; <t>β-ME,</t> <t>β-mercaptoethanol;</t> dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC
    β Mercaptoethanol βme, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol bme
    Chemical structures and electrospray product ion tandem mass spectra of the natural chemoprevention agents used in this study and their corresponding adducts with <t>β-mercaptoethanol</t> <t>(BME).</t> A) isoliquiritigenin; B) isoliquiritigenin-BME adduct;
    β Mercaptoethanol Bme, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Millipore 2xsds β mercaptoethanol
    Chemical structures and electrospray product ion tandem mass spectra of the natural chemoprevention agents used in this study and their corresponding adducts with <t>β-mercaptoethanol</t> <t>(BME).</t> A) isoliquiritigenin; B) isoliquiritigenin-BME adduct;
    2xsds β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore α β me atp
    Typical recording of arterial blood pressure (ABP) response to arterial injection of <t>α,β-me-ATP;</t> 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response
    α β Me Atp, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore β mercaptoethanol 2 hydroxyethylmercaptan
    Typical recording of arterial blood pressure (ABP) response to arterial injection of <t>α,β-me-ATP;</t> 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response
    β Mercaptoethanol 2 Hydroxyethylmercaptan, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore β mercaptoethanol molecular grade
    Typical recording of arterial blood pressure (ABP) response to arterial injection of <t>α,β-me-ATP;</t> 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response
    β Mercaptoethanol Molecular Grade, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore photoprotective agents β mercaptoethanol
    Typical recording of arterial blood pressure (ABP) response to arterial injection of <t>α,β-me-ATP;</t> 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response
    Photoprotective Agents β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol laemmli buffer
    Typical recording of arterial blood pressure (ABP) response to arterial injection of <t>α,β-me-ATP;</t> 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response
    β Mercaptoethanol Laemmli Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore buffer rlt β mercaptoethanol
    Typical recording of arterial blood pressure (ABP) response to arterial injection of <t>α,β-me-ATP;</t> 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response
    Buffer Rlt β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore oxygen scavenger β mercaptoethanol
    Typical recording of arterial blood pressure (ABP) response to arterial injection of <t>α,β-me-ATP;</t> 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response
    Oxygen Scavenger β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore agent 2 β mercaptoethanol 2me
    Effect of HgCl 2 , an inhibitor of AQPs function on swelling of spore of Rhizopus delemar . Treatment of R . delemar spores with 40 μM HgCl 2 for 5 min inhibited spore swelling and germination. Additional treatment (5 min) with 5 μM of the reducing agent <t>2-β-mercaptoethanol</t> (2ME) fully reversed the inhibition effect. Pictures were taken 15 min and 3 h, in the upper and lower rows, respectively, after incubation in water or sweet potato active fraction (SPAF). Bar = 100 μm.
    Agent 2 β Mercaptoethanol 2me, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol buffer solution
    Effect of HgCl 2 , an inhibitor of AQPs function on swelling of spore of Rhizopus delemar . Treatment of R . delemar spores with 40 μM HgCl 2 for 5 min inhibited spore swelling and germination. Additional treatment (5 min) with 5 μM of the reducing agent <t>2-β-mercaptoethanol</t> (2ME) fully reversed the inhibition effect. Pictures were taken 15 min and 3 h, in the upper and lower rows, respectively, after incubation in water or sweet potato active fraction (SPAF). Bar = 100 μm.
    β Mercaptoethanol Buffer Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol stock solution
    Effect of HgCl 2 , an inhibitor of AQPs function on swelling of spore of Rhizopus delemar . Treatment of R . delemar spores with 40 μM HgCl 2 for 5 min inhibited spore swelling and germination. Additional treatment (5 min) with 5 μM of the reducing agent <t>2-β-mercaptoethanol</t> (2ME) fully reversed the inhibition effect. Pictures were taken 15 min and 3 h, in the upper and lower rows, respectively, after incubation in water or sweet potato active fraction (SPAF). Bar = 100 μm.
    β Mercaptoethanol Stock Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 5x10 5 m β mercaptoethanol
    Effect of HgCl 2 , an inhibitor of AQPs function on swelling of spore of Rhizopus delemar . Treatment of R . delemar spores with 40 μM HgCl 2 for 5 min inhibited spore swelling and germination. Additional treatment (5 min) with 5 μM of the reducing agent <t>2-β-mercaptoethanol</t> (2ME) fully reversed the inhibition effect. Pictures were taken 15 min and 3 h, in the upper and lower rows, respectively, after incubation in water or sweet potato active fraction (SPAF). Bar = 100 μm.
    5x10 5 M β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore l glutamine β mercaptoethanol
    Effect of HgCl 2 , an inhibitor of AQPs function on swelling of spore of Rhizopus delemar . Treatment of R . delemar spores with 40 μM HgCl 2 for 5 min inhibited spore swelling and germination. Additional treatment (5 min) with 5 μM of the reducing agent <t>2-β-mercaptoethanol</t> (2ME) fully reversed the inhibition effect. Pictures were taken 15 min and 3 h, in the upper and lower rows, respectively, after incubation in water or sweet potato active fraction (SPAF). Bar = 100 μm.
    L Glutamine β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore β me atp
    Current responses evoked by <t>α,β-me</t> <t>ATP</t> in DiI-labeled DRG neurons. Traces (Panel A) and averaged data (Panel B) show that amplitude of currents (fast) activated by 30 µM of α,β-me ATP was inhibited with PPADS (0.1 mM), a P2X receptor antagonist. The inhibition with PPADS decreased over time after its application onto DRG neurons. * P
    β Me Atp, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol 100x
    Current responses evoked by <t>α,β-me</t> <t>ATP</t> in DiI-labeled DRG neurons. Traces (Panel A) and averaged data (Panel B) show that amplitude of currents (fast) activated by 30 µM of α,β-me ATP was inhibited with PPADS (0.1 mM), a P2X receptor antagonist. The inhibition with PPADS decreased over time after its application onto DRG neurons. * P
    β Mercaptoethanol 100x, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore specific β mercaptoethanol
    Current responses evoked by <t>α,β-me</t> <t>ATP</t> in DiI-labeled DRG neurons. Traces (Panel A) and averaged data (Panel B) show that amplitude of currents (fast) activated by 30 µM of α,β-me ATP was inhibited with PPADS (0.1 mM), a P2X receptor antagonist. The inhibition with PPADS decreased over time after its application onto DRG neurons. * P
    Specific β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore reagent β mercaptoethanol
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
    Reagent β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore reagents β mercaptoethanol camptothecin
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
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    Millipore β mercaptoethanol sigma chemical
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
    β Mercaptoethanol Sigma Chemical, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol ßme
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
    β Mercaptoethanol ßme, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore supplemental β mercaptoethanol bme
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
    Supplemental β Mercaptoethanol Bme, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol reducing agent
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
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    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
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    Millipore heat inactivated fbs β mercaptoethanol
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
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    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
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    Millipore β mercaptoethanol sigma penicillin
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
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    Image Search Results


    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; β-ME, β-mercaptoethanol; dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC

    Journal: Stem Cell Research & Therapy

    Article Title: Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function

    doi: 10.1186/s13287-018-0884-3

    Figure Lengend Snippet: Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; β-ME, β-mercaptoethanol; dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC

    Article Snippet: The experimental grouping and specific induction methods were as follows (Fig. ): (i) uASCs: undifferentiated ASCs (uASCs) at passage 2 were used; (ii–v) ASCs at passage 2 were pre-induced for 24 h with pre-induction medium I consisting of serum-free Dulbecco’s modified Eagle’s medium (DMEM) containing 1 mM β-mercaptoethanol (β-ME) (M3148; Sigma).

    Techniques: Derivative Assay, Staining, Flow Cytometry, Cytometry, Modification

    β-Mercaptoethanol inhibits ferroptosis induced by depletion of CD98hc/xCT transporter and restores CD98hc-null cell survival. A , WT and CD98hc-null ( KO ) fibroblast proliferation was compared in the absence (−) ( left panel ) and presence

    Journal: The Journal of Biological Chemistry

    Article Title: Amino Acid Transport Associated to Cluster of Differentiation 98 Heavy Chain (CD98hc) Is at the Cross-road of Oxidative Stress and Amino Acid Availability *

    doi: 10.1074/jbc.M115.704254

    Figure Lengend Snippet: β-Mercaptoethanol inhibits ferroptosis induced by depletion of CD98hc/xCT transporter and restores CD98hc-null cell survival. A , WT and CD98hc-null ( KO ) fibroblast proliferation was compared in the absence (−) ( left panel ) and presence

    Article Snippet: Wild-type and CD98hc-null mouse ES cells as well as corresponding ES-derived fibroblasts were cultured in complete DMEM high glucose (Gibco) medium supplemented with 10% v/v FBS (HyClone), 20 m m Hepes, pH 7.3, 100 μ m non-essential amino acids (Gibco), 2 m m l -glutamine (Gibco), and if not stated otherwise, 100 μ m β-ME (Sigma) at 37 °C and 5% v/v CO2 in an humidified incubator.

    Techniques:

    FTIR spectra in the frequencies of amide I vibrations ( a and b ) and FT-Raman spectra ( c – e ) measured at 20°C for 5% ( 1 ), 10% ( 2 ), and 15% ( 3 ) (w/w) Lanreotide acetate in water. The spectra 1′ and 2′ were obtained for Lanreotide concentration similar to the spectra 1 and 2, respectively, but in presence of β -mercaptoethanol (14 M). The spectrum ( 4 ) on d has been recorded for pure β -mercaptoethanol. The spectra 5 and 6 on e have been recorded for pure naphthalene and naphthalene in tetrahydrofurane, respectively.

    Journal: Biophysical Journal

    Article Title: Self-Association Process of a Peptide in Solution: From β-Sheet Filaments to Large Embedded Nanotubes

    doi:

    Figure Lengend Snippet: FTIR spectra in the frequencies of amide I vibrations ( a and b ) and FT-Raman spectra ( c – e ) measured at 20°C for 5% ( 1 ), 10% ( 2 ), and 15% ( 3 ) (w/w) Lanreotide acetate in water. The spectra 1′ and 2′ were obtained for Lanreotide concentration similar to the spectra 1 and 2, respectively, but in presence of β -mercaptoethanol (14 M). The spectrum ( 4 ) on d has been recorded for pure β -mercaptoethanol. The spectra 5 and 6 on e have been recorded for pure naphthalene and naphthalene in tetrahydrofurane, respectively.

    Article Snippet: Glycerol 99.9%, solid naphthalene 99%, and β -mercaptoethanol 14 M were purchased from Sigma (Paris, France).

    Techniques: Concentration Assay

    β-mercaptoethanol (2-ME) restored Ca 2 + response in KC in exposure to H 2 O 2 . ( A ) Ca 2+ signal in each group (pretreatment with vehicle, 500 μM H 2 O 2 , and 500 μM H 2 O 2 + 2-ME) was induced by addition of ATP (black bars). ( B , C ) Quantification of the black areas representing ATP-induced Ca 2+ signals ( B ) and the peak of the intracellular Ca 2+ responses ( C ) shown in ( A ) (N = 3). (D) Ca 2+ signal in each group (pretreatment with vehicle, 500 μM H 2 O 2 , 500 μM H 2 O 2 + 2-ME) was stimulated by photolysis-induced uncaging of caged-IP 3 . ( E ) Quantification of the black areas in ( D ) represent the amount of Ca 2+ released by the uncaged IP 3 (*P

    Journal: Scientific Reports

    Article Title: Hydrogen gas protects IP3Rs by reducing disulfide bridges in human keratinocytes under oxidative stress

    doi: 10.1038/s41598-017-03513-2

    Figure Lengend Snippet: β-mercaptoethanol (2-ME) restored Ca 2 + response in KC in exposure to H 2 O 2 . ( A ) Ca 2+ signal in each group (pretreatment with vehicle, 500 μM H 2 O 2 , and 500 μM H 2 O 2 + 2-ME) was induced by addition of ATP (black bars). ( B , C ) Quantification of the black areas representing ATP-induced Ca 2+ signals ( B ) and the peak of the intracellular Ca 2+ responses ( C ) shown in ( A ) (N = 3). (D) Ca 2+ signal in each group (pretreatment with vehicle, 500 μM H 2 O 2 , 500 μM H 2 O 2 + 2-ME) was stimulated by photolysis-induced uncaging of caged-IP 3 . ( E ) Quantification of the black areas in ( D ) represent the amount of Ca 2+ released by the uncaged IP 3 (*P

    Article Snippet: This possibility was examined using the reduction agent β-mercaptoethanol (2-ME; Sigma-Aldrich).

    Techniques:

    Chemical structures and electrospray product ion tandem mass spectra of the natural chemoprevention agents used in this study and their corresponding adducts with β-mercaptoethanol (BME). A) isoliquiritigenin; B) isoliquiritigenin-BME adduct;

    Journal: Analytical Biochemistry

    Article Title: Screening for Natural Chemoprevention Agents that Modify Human Keap1

    doi: 10.1016/j.ab.2011.10.028

    Figure Lengend Snippet: Chemical structures and electrospray product ion tandem mass spectra of the natural chemoprevention agents used in this study and their corresponding adducts with β-mercaptoethanol (BME). A) isoliquiritigenin; B) isoliquiritigenin-BME adduct;

    Article Snippet: Isoliquiritigenin, naringenin, Tris-HCl, sodium chloride, glycerol, formic acid, dimethyl sulfoxide, and β-mercaptoethanol (BME) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques:

    Typical recording of arterial blood pressure (ABP) response to arterial injection of α,β-me-ATP; 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Typical recording of arterial blood pressure (ABP) response to arterial injection of α,β-me-ATP; 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Injection

    Sympathetic and cardiovascular responses to arterial injection of α,β-me-ATP.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Sympathetic and cardiovascular responses to arterial injection of α,β-me-ATP.

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Injection

    Typical recording of renal sympathetic nerve activity (RSNA) and arterial blood pressure (AP) responses to stimulation of P2X. Arrowheads indicate a start of injections. Three dosages of α,β-methylene ATP (α,β-me-ATP) were

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Typical recording of renal sympathetic nerve activity (RSNA) and arterial blood pressure (AP) responses to stimulation of P2X. Arrowheads indicate a start of injections. Three dosages of α,β-methylene ATP (α,β-me-ATP) were

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Activity Assay

    Changes in RSNA and mean arterial pressure (MAP) in response to stimulation of afferent nerve P2X receptors. Three dosages of α,β-methylene ATP were injected into arterial blood supply of the hindlimb muscles of control rats ( n = 8) and

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Changes in RSNA and mean arterial pressure (MAP) in response to stimulation of afferent nerve P2X receptors. Three dosages of α,β-methylene ATP were injected into arterial blood supply of the hindlimb muscles of control rats ( n = 8) and

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Injection

    Top : effects of NGF infusion on blood pressure and heart rate responses to stimulation of P2X. α,β-me-ATP at 0.125 mM was injected into arterial blood supply of the muscles of control leg and experimental legs in 6 health rats. Control

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Top : effects of NGF infusion on blood pressure and heart rate responses to stimulation of P2X. α,β-me-ATP at 0.125 mM was injected into arterial blood supply of the muscles of control leg and experimental legs in 6 health rats. Control

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Injection

    Effect of HgCl 2 , an inhibitor of AQPs function on swelling of spore of Rhizopus delemar . Treatment of R . delemar spores with 40 μM HgCl 2 for 5 min inhibited spore swelling and germination. Additional treatment (5 min) with 5 μM of the reducing agent 2-β-mercaptoethanol (2ME) fully reversed the inhibition effect. Pictures were taken 15 min and 3 h, in the upper and lower rows, respectively, after incubation in water or sweet potato active fraction (SPAF). Bar = 100 μm.

    Journal: PLoS ONE

    Article Title: The Role of Aquaporins in pH-Dependent Germination of Rhizopus delemar Spores

    doi: 10.1371/journal.pone.0150543

    Figure Lengend Snippet: Effect of HgCl 2 , an inhibitor of AQPs function on swelling of spore of Rhizopus delemar . Treatment of R . delemar spores with 40 μM HgCl 2 for 5 min inhibited spore swelling and germination. Additional treatment (5 min) with 5 μM of the reducing agent 2-β-mercaptoethanol (2ME) fully reversed the inhibition effect. Pictures were taken 15 min and 3 h, in the upper and lower rows, respectively, after incubation in water or sweet potato active fraction (SPAF). Bar = 100 μm.

    Article Snippet: Spores were incubated with 5 μM of the reducing agent 2-β-mercaptoethanol (2ME) (Sigma-Aldrich) for 5 min to reverse the inhibition effect.

    Techniques: Inhibition, Incubation

    Current responses evoked by α,β-me ATP in DiI-labeled DRG neurons. Traces (Panel A) and averaged data (Panel B) show that amplitude of currents (fast) activated by 30 µM of α,β-me ATP was inhibited with PPADS (0.1 mM), a P2X receptor antagonist. The inhibition with PPADS decreased over time after its application onto DRG neurons. * P

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    Article Title: Bradykinin Contributes to Sympathetic and Pressor Responses Evoked by Activation of Skeletal Muscle Afferents P2X in Heart Failure

    doi: 10.1159/000447906

    Figure Lengend Snippet: Current responses evoked by α,β-me ATP in DiI-labeled DRG neurons. Traces (Panel A) and averaged data (Panel B) show that amplitude of currents (fast) activated by 30 µM of α,β-me ATP was inhibited with PPADS (0.1 mM), a P2X receptor antagonist. The inhibition with PPADS decreased over time after its application onto DRG neurons. * P

    Article Snippet: To examine effects of BK on α,β-me ATP, BK (0.5, 1.0 and 1.5 µg/kg; Sigma-Aldrich) was infused into the femoral artery 20 min before α,β-me ATP, respectively.

    Techniques: Labeling, Inhibition

    Effects of BK on responses of the RSNA, MAP and HR induced by arterial injection of 0.125 mM of α,β-me ATP. Panel A, B and C represent the responses of RSNA, MAP and HR, respectively. * P

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    Article Title: Bradykinin Contributes to Sympathetic and Pressor Responses Evoked by Activation of Skeletal Muscle Afferents P2X in Heart Failure

    doi: 10.1159/000447906

    Figure Lengend Snippet: Effects of BK on responses of the RSNA, MAP and HR induced by arterial injection of 0.125 mM of α,β-me ATP. Panel A, B and C represent the responses of RSNA, MAP and HR, respectively. * P

    Article Snippet: To examine effects of BK on α,β-me ATP, BK (0.5, 1.0 and 1.5 µg/kg; Sigma-Aldrich) was infused into the femoral artery 20 min before α,β-me ATP, respectively.

    Techniques: Injection

    Effects of BK on α,β-me ATP-induced currents in DiI-labeled DRG neurons. Typical traces (Panel A) and averaged data (Panel B C): α,β-me ATP evoked greater peak current amplitude (fast and slow) in HF rats. † P

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    Article Title: Bradykinin Contributes to Sympathetic and Pressor Responses Evoked by Activation of Skeletal Muscle Afferents P2X in Heart Failure

    doi: 10.1159/000447906

    Figure Lengend Snippet: Effects of BK on α,β-me ATP-induced currents in DiI-labeled DRG neurons. Typical traces (Panel A) and averaged data (Panel B C): α,β-me ATP evoked greater peak current amplitude (fast and slow) in HF rats. † P

    Article Snippet: To examine effects of BK on α,β-me ATP, BK (0.5, 1.0 and 1.5 µg/kg; Sigma-Aldrich) was infused into the femoral artery 20 min before α,β-me ATP, respectively.

    Techniques: Labeling

    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with β-mercaptoethanol; 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).

    Journal: PLoS ONE

    Article Title: New Cysteine-Rich Ice-Binding Protein Secreted from Antarctic Microalga, Chloromonas sp.

    doi: 10.1371/journal.pone.0154056

    Figure Lengend Snippet: In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with β-mercaptoethanol; 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).

    Article Snippet: Biochemical characteristics of native ChloroIBP The change of in-gel mobility of ChloroIBP by the reducing reagent β-mercaptoethanol (Sigma) was analyzed by immunoblotting with a ChloroIBP-specific polyclonal antibody.

    Techniques: Recombinant, SDS Page, Silver Staining, Staining