β-glycerophosphate Search Results


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  • 99
    Millipore β glycerophosphate
    Measurements of cell proliferation by alamar blue assay on DPSC ( a ), Saos-2 ( b ) and HEPM ( c ) cells cultured in control medium (CM) supplemented with different components of the OIM medium such as: ascorbic acid (AA), <t>β-glycerophosphate</t> (β-GLY), dexamethasone (DEX), vitamin D3 (D3 VIT). Those components which decreased proliferation (β-glycerophosphate and dexamethasone) were combined with BMP-2 and were examined on DPSC ( d ), Saos-2 ( e ) and HEPM ( f ) cells. Error bars represent standard deviation calculated from three parallel measurements. Statistical analysis was performed using ANOVA followed by Bonferroni statistical test. P
    β Glycerophosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology β glycerophosphate
    ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and <t>β‐glycerophosphate</t> for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P
    β Glycerophosphate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FUJIFILM β glycerophosphate
    Effect of toddaculin on osteoblast mineralization in ascorbic acid and <t>β-glycerophosphate-treated</t> MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P
    β Glycerophosphate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 93/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher β glycerophosphate
    Effect of toddaculin on osteoblast mineralization in ascorbic acid and <t>β-glycerophosphate-treated</t> MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P
    β Glycerophosphate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co β glycerophosphate
    Effect of toddaculin on osteoblast mineralization in ascorbic acid and <t>β-glycerophosphate-treated</t> MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P
    β Glycerophosphate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad β glycerophosphate
    Effect of toddaculin on osteoblast mineralization in ascorbic acid and <t>β-glycerophosphate-treated</t> MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P
    β Glycerophosphate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore m β glycerophosphate
    hBMSC proliferation in medium with and without osteogenic supplements. (A, B) BMSC colony formation in primary bone marrow cell cultures in medium with various concentrations of dexamethasone (Dex), ascopbate (Asc), and <t>sodium-β-glycerophosphate</t> (β-GP). **, p
    M β Glycerophosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 759 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Nacalai β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Valiant β glycerophosphate
    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
    β Glycerophosphate, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applichem β glycerophosphate
    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
    β Glycerophosphate, supplied by Applichem, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology sodium β glycerophosphate
    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
    Sodium β Glycerophosphate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co sodium β glycerophosphate
    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
    Sodium β Glycerophosphate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beijing Solarbio Science β glycerophosphate
    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
    β Glycerophosphate, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Measurements of cell proliferation by alamar blue assay on DPSC ( a ), Saos-2 ( b ) and HEPM ( c ) cells cultured in control medium (CM) supplemented with different components of the OIM medium such as: ascorbic acid (AA), β-glycerophosphate (β-GLY), dexamethasone (DEX), vitamin D3 (D3 VIT). Those components which decreased proliferation (β-glycerophosphate and dexamethasone) were combined with BMP-2 and were examined on DPSC ( d ), Saos-2 ( e ) and HEPM ( f ) cells. Error bars represent standard deviation calculated from three parallel measurements. Statistical analysis was performed using ANOVA followed by Bonferroni statistical test. P

    Journal: Human Cell

    Article Title: Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin

    doi: 10.1007/s13577-018-0202-5

    Figure Lengend Snippet: Measurements of cell proliferation by alamar blue assay on DPSC ( a ), Saos-2 ( b ) and HEPM ( c ) cells cultured in control medium (CM) supplemented with different components of the OIM medium such as: ascorbic acid (AA), β-glycerophosphate (β-GLY), dexamethasone (DEX), vitamin D3 (D3 VIT). Those components which decreased proliferation (β-glycerophosphate and dexamethasone) were combined with BMP-2 and were examined on DPSC ( d ), Saos-2 ( e ) and HEPM ( f ) cells. Error bars represent standard deviation calculated from three parallel measurements. Statistical analysis was performed using ANOVA followed by Bonferroni statistical test. P

    Article Snippet: Osteoinductive medium (OIM) was prepared by supplementing CM with 10 mM β-glycerophosphate (Sigma Aldrich, G9891), 50 µg/ml ascorbic acid (Sigma Aldrich, 1043003), 0.1 µM dexamethasone (Sigma Aldrich, D4902) and 50 nM vitamin D3 (Sigma Aldrich, 740292).

    Techniques: Alamar Blue Assay, Cell Culture, Standard Deviation

    Memo deletion affects intracellular redox state and SDS stability of femoral ALP. ( A ) The oxidized to reduced NAD ratio (NAD+/NADH) was increased in marrow‐free femurs of Memo cKO mice relative to controls. ( B ) Oxidation‐sensitive bioconjugation with AMS revealed indifferently oxidized TNAP in marrow‐free femurs of Memo cKO and controls on native gel electrophoresis with or without reduction by 2% βME. After SDS‐PAGE separation, TNAP dimers were 31‐fold and monomers fourfold less abundant in bone of Memo cKO compared to controls ( C ); densitometric quantification in monomers ( D ) and dimers ( E ). In‐gel activity staining ( F ) using SDS‐PAGE showed that the presumed ALP dimers in C correspond to the bioactive femoral ALP enzyme. In‐gel ALP activity was reduced in marrow‐free femurs of Memo cKO ( F ; quantification in G ). SDS = sodium dodecyl sulfate; AMS = 4‐acetamido‐4′‐maleimidylstilbene‐2,2′‐disulfonic acid; TNAP = tissue‐nonspecific alkaline phosphatase; βME = beta‐mercaptoethanol.

    Journal: JBMR Plus

    Article Title: Redox‐Dependent Bone Alkaline Phosphatase Dysfunction Drives Part of the Complex Bone Phenotype in Mice Deficient for Memo1

    doi: 10.1002/jbm4.10034

    Figure Lengend Snippet: Memo deletion affects intracellular redox state and SDS stability of femoral ALP. ( A ) The oxidized to reduced NAD ratio (NAD+/NADH) was increased in marrow‐free femurs of Memo cKO mice relative to controls. ( B ) Oxidation‐sensitive bioconjugation with AMS revealed indifferently oxidized TNAP in marrow‐free femurs of Memo cKO and controls on native gel electrophoresis with or without reduction by 2% βME. After SDS‐PAGE separation, TNAP dimers were 31‐fold and monomers fourfold less abundant in bone of Memo cKO compared to controls ( C ); densitometric quantification in monomers ( D ) and dimers ( E ). In‐gel activity staining ( F ) using SDS‐PAGE showed that the presumed ALP dimers in C correspond to the bioactive femoral ALP enzyme. In‐gel ALP activity was reduced in marrow‐free femurs of Memo cKO ( F ; quantification in G ). SDS = sodium dodecyl sulfate; AMS = 4‐acetamido‐4′‐maleimidylstilbene‐2,2′‐disulfonic acid; TNAP = tissue‐nonspecific alkaline phosphatase; βME = beta‐mercaptoethanol.

    Article Snippet: After 7 days, osteogenic differentiation was initiated with alpha‐MEM + FCS 10% + penicillin/streptomycin 1% + beta‐glycerophosphate 10mM (Sigma) + L‐Ascorbic acid 50 μg/mL (Fluka).

    Techniques: ALP Assay, Mouse Assay, Affinity Magnetic Separation, Nucleic Acid Electrophoresis, SDS Page, Activity Assay, Staining

    Effects of methylsulfonylmethane (MSM) on viability in osteoblast-like cells and MSCs. MG-63 and UMR-106 cells exposed to control condition without MSM or growth facilitated condition with increasing concentration of MSM for 24 h. C3H10T1/2 cells and mesenchymal stem cells grown in the osteogenic media (10 mM sodium β-glycerophosphate and 50 µg/ml ascorbic acid) and exposed to control condition or growth facilitated condition for 21 days. After culture, cell viability was evaluated using MTT assay. Data shown are representative of three independent experiments.

    Journal: PLoS ONE

    Article Title: MSM Enhances GH Signaling via the Jak2/STAT5b Pathway in Osteoblast-Like Cells and Osteoblast Differentiation through the Activation of STAT5b in MSCs

    doi: 10.1371/journal.pone.0047477

    Figure Lengend Snippet: Effects of methylsulfonylmethane (MSM) on viability in osteoblast-like cells and MSCs. MG-63 and UMR-106 cells exposed to control condition without MSM or growth facilitated condition with increasing concentration of MSM for 24 h. C3H10T1/2 cells and mesenchymal stem cells grown in the osteogenic media (10 mM sodium β-glycerophosphate and 50 µg/ml ascorbic acid) and exposed to control condition or growth facilitated condition for 21 days. After culture, cell viability was evaluated using MTT assay. Data shown are representative of three independent experiments.

    Article Snippet: The anti-phosphotyrosine monoclonal antibody 4G10 and phospho-STAT5b were obtained from Upstate Biotechnology (Lake Placid, NY), The anti-actin antibody , Methyl sulfonemethane (MSM), Alizarin red S, ascorbic acid phosphate, β-glycerophosphate disodium salt hydrate, L-glutamine, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma Chemical Co. (St. Louis, MO).

    Techniques: Concentration Assay, MTT Assay

    Strontium indirectly inhibited osteoclastogenesis by enhancing LRP6/β-catenin/OPG signal in MC3T3-E1 cells. Relative expression levels of OPG ( a ), RBPJ ( b ) in MC3T3-E1 cells treated with different concentrations of strontium. c Relative expression levels of TRAP in RAW264.7 cells treated with strontium. d Relative expression levels of OPG in the procedure of MC3T3-E1 cell differentiation induced by β-Glycerophosphate disodium salt and L-Ascorbic acid. RNA extraction was conducted after the cells were induced for four or five days. The results are expressed as mean ± SD. * p

    Journal: Journal of Cell Communication and Signaling

    Article Title: Strontium inhibits osteoclastogenesis by enhancing LRP6 and β-catenin-mediated OPG targeted by miR-181d-5p

    doi: 10.1007/s12079-018-0478-y

    Figure Lengend Snippet: Strontium indirectly inhibited osteoclastogenesis by enhancing LRP6/β-catenin/OPG signal in MC3T3-E1 cells. Relative expression levels of OPG ( a ), RBPJ ( b ) in MC3T3-E1 cells treated with different concentrations of strontium. c Relative expression levels of TRAP in RAW264.7 cells treated with strontium. d Relative expression levels of OPG in the procedure of MC3T3-E1 cell differentiation induced by β-Glycerophosphate disodium salt and L-Ascorbic acid. RNA extraction was conducted after the cells were induced for four or five days. The results are expressed as mean ± SD. * p

    Article Snippet: The differentiation and osteogenesis of MC3T3-E1 cells were induced by the growth medium supplemented with 10 mM β-Glycerophosphate disodium salt (Sigma-Aldrich, St. Louis, USA) and 50 μg/ml L-Ascorbic acid (Sigma-Aldrich, St. Louis, USA).

    Techniques: Expressing, Cell Differentiation, RNA Extraction

    ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and β‐glycerophosphate for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P

    Journal: FEBS Open Bio

    Article Title: ADAMTS7 degrades Comp to fuel BMP2‐dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas

    doi: 10.1002/2211-5463.12939

    Figure Lengend Snippet: ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and β‐glycerophosphate for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P

    Article Snippet: Alizarin red staining MG63 cells were stimulated by 0.5 mm β‐glycerophosphate (#sc‐220452; Santa Cruz Biotechnology) and 50 mg·L−1 l‐ascorbic acid (#A92902; Sigma‐Aldrich, St Louis, MO, USA) for 21 days to induce osteogenic differentiation.

    Techniques: Staining

    Effect of toddaculin on osteoblast mineralization in ascorbic acid and β-glycerophosphate-treated MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P

    Journal: PLoS ONE

    Article Title: Toddaculin, Isolated from of Toddalia asiatica (L.) Lam., Inhibited Osteoclastogenesis in RAW 264 Cells and Enhanced Osteoblastogenesis in MC3T3-E1 Cells

    doi: 10.1371/journal.pone.0127158

    Figure Lengend Snippet: Effect of toddaculin on osteoblast mineralization in ascorbic acid and β-glycerophosphate-treated MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P

    Article Snippet: Ascorbic acid and β-glycerophosphate were purchased from Wako Pure Chemical (Osaka, Japan).

    Techniques: Staining

    hBMSC proliferation in medium with and without osteogenic supplements. (A, B) BMSC colony formation in primary bone marrow cell cultures in medium with various concentrations of dexamethasone (Dex), ascopbate (Asc), and sodium-β-glycerophosphate (β-GP). **, p

    Journal: Journal of tissue engineering and regenerative medicine

    Article Title: In vivo formation of bone and hematopoietic territories by transplanted human bone marrow stromal cells generated in medium with and without osteogenic supplements

    doi: 10.1002/term.515

    Figure Lengend Snippet: hBMSC proliferation in medium with and without osteogenic supplements. (A, B) BMSC colony formation in primary bone marrow cell cultures in medium with various concentrations of dexamethasone (Dex), ascopbate (Asc), and sodium-β-glycerophosphate (β-GP). **, p

    Article Snippet: The culture medium contained either no osteogenic supplements, or various concentrations of dexamethasone (Dex), ascorbic acid, or sodium-β-glycerophosphate (β-GP, all from Sigma, St. Louis, MO), or a combination of Dex at 1×10-8 M and L-ascorbic acid phosphate magnesium salt n-hydrate (AscP, Wako, Osaka, Japan) at 10-4 M. Cultivation was performed at 37 °C in a humidified atmosphere of 5% CO2 in air, with no medium replacements.

    Techniques:

    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+ cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+ supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+ supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP, β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Article Snippet: Experimental design For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture, Isolation, Spectroscopy

    Impact of Mg 2+  on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+  (gray line). Of note: as the BGP culture supplemented with Mg 2+  contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP,  β -glycerophosphate.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Impact of Mg 2+ on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+ (gray line). Of note: as the BGP culture supplemented with Mg 2+ contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP, β -glycerophosphate.

    Article Snippet: Experimental design For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture

    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using β-glycerophosphate plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p

    Journal: Redox Biology

    Article Title: KLF2 regulates dental pulp-derived stem cell differentiation through the induction of mitophagy and altering mitochondrial metabolism

    doi: 10.1016/j.redox.2020.101622

    Figure Lengend Snippet: OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using β-glycerophosphate plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p

    Article Snippet: Alizarin Red Solution (2003999) was obtained from Chemicon international. β Glycerophosphate (157241) was obtained from MP biomedical.

    Techniques: Expressing, Quantitative RT-PCR, Isolation

    Knockdown of KLF2 or ATG7 or BECN1 reduced autophagy-related molecules and OB differentiation-related markers. KLF2 or ATG7 or BECN1 was knocked down in DPSCs and cells were cultured in β-glycerophosphate plus l -ascorbic acid-containing medium. A. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of KLF2. B. MDC staining for detection of autophagy vesicles after knockdown of KLF2. C. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of ATG7. D. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of BECN1.

    Journal: Redox Biology

    Article Title: KLF2 regulates dental pulp-derived stem cell differentiation through the induction of mitophagy and altering mitochondrial metabolism

    doi: 10.1016/j.redox.2020.101622

    Figure Lengend Snippet: Knockdown of KLF2 or ATG7 or BECN1 reduced autophagy-related molecules and OB differentiation-related markers. KLF2 or ATG7 or BECN1 was knocked down in DPSCs and cells were cultured in β-glycerophosphate plus l -ascorbic acid-containing medium. A. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of KLF2. B. MDC staining for detection of autophagy vesicles after knockdown of KLF2. C. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of ATG7. D. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of BECN1.

    Article Snippet: Alizarin Red Solution (2003999) was obtained from Chemicon international. β Glycerophosphate (157241) was obtained from MP biomedical.

    Techniques: Cell Culture, Western Blot, Staining