β-glycerophosphate Search Results


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  • 99
    Thermo Fisher β glycerophosphate
    β Glycerophosphate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Thermo Fisher
    Average 99 stars, based on 799 article reviews
    Price from $9.99 to $1999.99
    β glycerophosphate - by Bioz Stars, 2021-01
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    99
    Millipore β glycerophosphate
    BMP2 overcomes inhibited effect of MSC-CM on β-GP-induced VSMC calcification. a Microscopic views (×100) of Alizarin Red staining. b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. Expression of Msx2 ( d ), Runx2 ( e ), and osteocalcin ( f ) mRNA assessed by rt-PCR. g Cell lysates analyzed by western blot analysis with anti-Runx2 and p-Smad1/5/8. β-GP <t>beta-glycerophosphate,</t> DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2
    β Glycerophosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    FUJIFILM β glycerophosphate
    BMP2 overcomes inhibited effect of MSC-CM on β-GP-induced VSMC calcification. a Microscopic views (×100) of Alizarin Red staining. b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. Expression of Msx2 ( d ), Runx2 ( e ), and osteocalcin ( f ) mRNA assessed by rt-PCR. g Cell lysates analyzed by western blot analysis with anti-Runx2 and p-Smad1/5/8. β-GP <t>beta-glycerophosphate,</t> DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2
    β Glycerophosphate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β glycerophosphate - by Bioz Stars, 2021-01
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    92
    Merck & Co β glycerophosphate
    BMP2 overcomes inhibited effect of MSC-CM on β-GP-induced VSMC calcification. a Microscopic views (×100) of Alizarin Red staining. b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. Expression of Msx2 ( d ), Runx2 ( e ), and osteocalcin ( f ) mRNA assessed by rt-PCR. g Cell lysates analyzed by western blot analysis with anti-Runx2 and p-Smad1/5/8. β-GP <t>beta-glycerophosphate,</t> DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2
    β Glycerophosphate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad β glycerophosphate
    BMP2 overcomes inhibited effect of MSC-CM on β-GP-induced VSMC calcification. a Microscopic views (×100) of Alizarin Red staining. b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. Expression of Msx2 ( d ), Runx2 ( e ), and osteocalcin ( f ) mRNA assessed by rt-PCR. g Cell lysates analyzed by western blot analysis with anti-Runx2 and p-Smad1/5/8. β-GP <t>beta-glycerophosphate,</t> DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2
    β Glycerophosphate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore β glycerophosphate disodium
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate Disodium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nacalai β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Valiant β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Applichem β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Applichem, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Tokyo Chemical Industry β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck & Co sodium β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    Sodium β Glycerophosphate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sodium β glycerophosphate - by Bioz Stars, 2021-01
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    92
    Beijing Solarbio Science β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    β Glycerophosphate is a protein phosphatase inhibitor that acts as a phosphate group donor in matrix mineralization studies It promotes bone matrix mineralization when delivered to osteoblasts by providing a
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    N/A
    HepG2 Cell Nuclear Extract W09 001 GJ6
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    Hep G2 Whole Cell Lysate W09 001 GJ5
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    N/A
    β Glycerophosphate Disodium Salt Pentahydrate is a simple phosphate donor and is consequently a potent inhibitor of phosphatase This compound promotes bone matrix mineralization when delivered to osteoblasts by providing
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    Image Search Results


    BMP2 overcomes inhibited effect of MSC-CM on β-GP-induced VSMC calcification. a Microscopic views (×100) of Alizarin Red staining. b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. Expression of Msx2 ( d ), Runx2 ( e ), and osteocalcin ( f ) mRNA assessed by rt-PCR. g Cell lysates analyzed by western blot analysis with anti-Runx2 and p-Smad1/5/8. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: BMP2 overcomes inhibited effect of MSC-CM on β-GP-induced VSMC calcification. a Microscopic views (×100) of Alizarin Red staining. b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. Expression of Msx2 ( d ), Runx2 ( e ), and osteocalcin ( f ) mRNA assessed by rt-PCR. g Cell lysates analyzed by western blot analysis with anti-Runx2 and p-Smad1/5/8. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Modification, Derivative Assay

    Noggin, BMP2 antagonist, suppresses VSMC calcification by inhibiting Smad1/5/8 signaling. VSMCs pretreated with Noggin (100 ng/ml) or vehicle for 2 h followed by incubation with 10 mM β-GP. a Alizarin Red staining (×100). b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. mRNA expression of ( d ) BMP2, ( e ) Msx2, ( f ) Runx2, and ( g ) osteocalcin analyzed by rt-PCR. h Protein expression of BMP2 and phospho-Smad1/5/8 performed by western blot analysis. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, DMEM Noggin+β-GP DMEM medium containing 10 mM β-GP and 100 ng/ml Noggin, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: Noggin, BMP2 antagonist, suppresses VSMC calcification by inhibiting Smad1/5/8 signaling. VSMCs pretreated with Noggin (100 ng/ml) or vehicle for 2 h followed by incubation with 10 mM β-GP. a Alizarin Red staining (×100). b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. mRNA expression of ( d ) BMP2, ( e ) Msx2, ( f ) Runx2, and ( g ) osteocalcin analyzed by rt-PCR. h Protein expression of BMP2 and phospho-Smad1/5/8 performed by western blot analysis. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, DMEM Noggin+β-GP DMEM medium containing 10 mM β-GP and 100 ng/ml Noggin, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Incubation, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Modification

    β-GP induced calcium deposition in VSMCs in a dose-dependent manner. VSMCs cultured in normal medium or in calcification medium with 10, 20 and 40 mmol/l of β-GP for 14 days. a Alizarin Red staining of microscopic views (×100). b Formic acid 10% used to elute Alizarin Red dye and quantification of Alizarin Red staining measured with a microplate reader and normalized to total protein content. c Calcium content shown as mean ± standard deviation of three independent experiments conducted in duplicate. β-GP beta-glycerophosphate, OD optical density

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: β-GP induced calcium deposition in VSMCs in a dose-dependent manner. VSMCs cultured in normal medium or in calcification medium with 10, 20 and 40 mmol/l of β-GP for 14 days. a Alizarin Red staining of microscopic views (×100). b Formic acid 10% used to elute Alizarin Red dye and quantification of Alizarin Red staining measured with a microplate reader and normalized to total protein content. c Calcium content shown as mean ± standard deviation of three independent experiments conducted in duplicate. β-GP beta-glycerophosphate, OD optical density

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Cell Culture, Staining, Standard Deviation

    MSC-CM inhibits β-GP-induced calcium deposition in VSMCs. Confluent cells incubated with normal medium or calcification medium with or without MSC-CM for 14 days. Calcification induced by addition of β-GP (10 mM). a Representative Alizarin Red staining of microscopic views (×100, upper) and plates (lower). b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, NC DMEM medium, OD optical density

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: MSC-CM inhibits β-GP-induced calcium deposition in VSMCs. Confluent cells incubated with normal medium or calcification medium with or without MSC-CM for 14 days. Calcification induced by addition of β-GP (10 mM). a Representative Alizarin Red staining of microscopic views (×100, upper) and plates (lower). b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, NC DMEM medium, OD optical density

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Incubation, Staining, Modification, Derivative Assay

    MSC-CM blocks BMP2–Smad1/5/8 signaling pathways. Confluent VSMCs incubated with different media in different groups for 7 days. a Effect of MSC-CM on BMP-2 mRNA expression. b Representative immunoblot of BMP2. c BMP2 protein quantified by densitometry. Levels of ( d ) BMPR1a, ( e ) BMPR1b, and ( f ) BMPR2 mRNA determined by rt-PCR. g Phosphorylation of Smad1/5/8 in whole cells. h Phosphorylation of Smad1/5/8 in nucleus and cytoplasm. β-GP beta-glycerophosphate, BMP-2 bone morphogenetic protein-2, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, NC DMEM medium

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: MSC-CM blocks BMP2–Smad1/5/8 signaling pathways. Confluent VSMCs incubated with different media in different groups for 7 days. a Effect of MSC-CM on BMP-2 mRNA expression. b Representative immunoblot of BMP2. c BMP2 protein quantified by densitometry. Levels of ( d ) BMPR1a, ( e ) BMPR1b, and ( f ) BMPR2 mRNA determined by rt-PCR. g Phosphorylation of Smad1/5/8 in whole cells. h Phosphorylation of Smad1/5/8 in nucleus and cytoplasm. β-GP beta-glycerophosphate, BMP-2 bone morphogenetic protein-2, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, NC DMEM medium

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Incubation, Expressing, Reverse Transcription Polymerase Chain Reaction, Modification, Derivative Assay

    MSC-CM abolished β-GP-induced ALP activity and expression of specific osteogenic markers in VSMCs. Confluent VSMCs incubated with different media in different groups. a ALP activity measured after 3 days by ALP kit, normalized to cellular protein contents. Levels of Msx2 ( b ), Runx2 ( c ) and Osteocalcin ( d ) mRNA determined after 7 days by rt-PCR. e Representative immunoblot of Runx2. f Runx2 protein quantified by densitometry. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, Runx2 Runt-related transcription factor 2

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: MSC-CM abolished β-GP-induced ALP activity and expression of specific osteogenic markers in VSMCs. Confluent VSMCs incubated with different media in different groups. a ALP activity measured after 3 days by ALP kit, normalized to cellular protein contents. Levels of Msx2 ( b ), Runx2 ( c ) and Osteocalcin ( d ) mRNA determined after 7 days by rt-PCR. e Representative immunoblot of Runx2. f Runx2 protein quantified by densitometry. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, Runx2 Runt-related transcription factor 2

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: ALP Assay, Activity Assay, Expressing, Incubation, Reverse Transcription Polymerase Chain Reaction, Modification, Derivative Assay

    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+ cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+ supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+ supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP, β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Article Snippet: Experimental design For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture, Isolation, Spectroscopy

    Impact of Mg 2+  on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+  (gray line). Of note: as the BGP culture supplemented with Mg 2+  contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP,  β -glycerophosphate.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Impact of Mg 2+ on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+ (gray line). Of note: as the BGP culture supplemented with Mg 2+ contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP, β -glycerophosphate.

    Article Snippet: Experimental design For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture